Click here to switch to the map view.

The map label for this gene is ilvI [H]

Identifier: 182679372

GI number: 182679372

Start: 2761107

End: 2762867

Strand: Reverse

Name: ilvI [H]

Synonym: Bind_2420

Alternate gene names: 182679372

Gene position: 2762867-2761107 (Counterclockwise)

Preceding gene: 182679373

Following gene: 182679371

Centisome position: 66.25

GC content: 57.07

Gene sequence:

>1761_bases
ATGTCCGAGAAAATGACCGGCGCACAAATGGTTGTCACGGCTCTGAAAGATCAGGGCGTGGACACGATTTTCGGTTATCC
GGGCGGCGCCGTCCTACCGATCTATGATGCTTTGTTCCATCAGAACAGCGTCAAACATGTCCTCGTTCGTCACGAGCAGG
GCGCTGTCCATGCGGCGGAAGGTTATGCGCGCTCCAGCGGCAAAATCGGCGTGGTTCTGGTGACGTCGGGACCCGGCGCG
ACGAATGCCGTCACCGGCCTGACTGACGCTCTTCTCGATTCCATTCCGCTTGTTTGCATTACGGGGCAGGTGCCGACGCA
TCTCATCGGCTCCGACGCCTTTCAGGAATGCGATACGGTGGGTATTACCCGCCATTGCACGAAACATAATTATCTCGTTC
GCCAGGTCGAGGATCTGCCTCGCATCCTGCATGAGGCTTTTTATGTCGCGCAGCATGGCCGGCCGGGGCCGGTGGTGATT
GATATCCCCAAGGATGTGCAATTCGCGCTGGGACTCTATGCGGGTCCTGAAAATATTGAGCACAAGACCTATAAGCCGGC
GTTCAAGGGTGATCTCGGCCGCATCGACGAGGCGCTGAGCTGGATCGCGGCGGCCAAGCGCCCGATCTTCTATACCGGTG
GTGGCATCATCAATTCAGGACCCGAGGCCTCCGCCTTGCTGCGGGAACTTGTTGATTTGACCGGCGCGCCGATCACCTCC
ACCTTGATGGGGCTCGGCGCCTATCCCGCTTCCTCTCCGCGTTGGCTCGGCATGCTGGGCATGCATGGAACCTACGAAGC
GAATAATGCCATGCATGATTGCGATCTGATGATCGCCATCGGTGCGCGTTTCGACGATCGTATTACCGGCCGGATCGATG
CTTTTTCTCCCGGCTCGCGCAAAATCCATATCGATATTGATCCATCCTCTATCAACAAGACCGTGAAGGTCGATCTTGGT
ATTGTCGGCGATTGTACCGCCGTCTTGCGGGATATGGTCGCGCGCTGGAAGGAACGGCATCTGGCCTGCGACGCGGCGGT
GCTCCAAAAATGGTGGCAGGAGATCGATCATTGGCGGGCCCGCAAATCGCTTGCCTATCGCGCGTCGAACAAGGTGATCA
AACCGCAATATGCCATTCAACGTCTGTTCGAACTGACGCGCGGCCGCGACACCTATATTTCGACCGAGGTCGGTCAGCAT
CAGATGTGGGCGGCACAGCATTATCATTTCGACGAACCCAATCGCTGGATGACGTCCGGCGGTCTCGGCACCATGGGTTA
CGGCTTGCCGGCGGCCATTGGCGCGCAGATGGCGCATCCGGGCTCGCTCGTCGTCGATATTGCTGGCGAGGCGTCGATCC
TGATGAACATTCAGGAACTTTCGACCGCTGTTCAGTTCCGGTTGCCGGTCAAGATTTTCATCCTCAACAATCAATATATG
GGCATGGTGCGCCAATGGCAGGAACTGCTGCATGGCGGCCGTTATTCCGAGAGTTATTCGGAAGCTCTGCCCGATTTCGT
GAAACTGGCCGAGGCCTATGGCTGTCATGGTATCCGTTGCGCCGATCCGAACACACTCGACGCCGCGATTCTCGAAATGA
TAGAAACCCCGAAGCCGGTTTTGTTCGATTGCATTGTCGATCGGGAGGAAAATTGCCTGCCGATGATTCCCTCGGGCAAG
GCGCATAATGAAATGATCCTGCCCGATATGGAAACGGAACTCGGGACGGCGATCGATGCCGCCGGCAAAATATTGGTGTG
A

Upstream 100 bases:

>100_bases
CTCGAGGCCCTCCGGGGCCTTTTTTATTGCTTTCCATCCCATTCTCTAAAAATTCATGACCCATGAAGGCATTCGGCTCA
AAAATGCCGCGGAGAATAAA

Downstream 100 bases:

>100_bases
TGATGAATGCTCCTGCCGCTCCCCTCTCGCCTTATTCCTCGGCACCCGCTGCCTCGAAGATCGAGGTGCATACGCTCTCC
GTCCTGGTCGATAACGAGCC

Product: acetolactate synthase, large subunit, biosynthetic type

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 586; Mature: 585

Protein sequence:

>586_residues
MSEKMTGAQMVVTALKDQGVDTIFGYPGGAVLPIYDALFHQNSVKHVLVRHEQGAVHAAEGYARSSGKIGVVLVTSGPGA
TNAVTGLTDALLDSIPLVCITGQVPTHLIGSDAFQECDTVGITRHCTKHNYLVRQVEDLPRILHEAFYVAQHGRPGPVVI
DIPKDVQFALGLYAGPENIEHKTYKPAFKGDLGRIDEALSWIAAAKRPIFYTGGGIINSGPEASALLRELVDLTGAPITS
TLMGLGAYPASSPRWLGMLGMHGTYEANNAMHDCDLMIAIGARFDDRITGRIDAFSPGSRKIHIDIDPSSINKTVKVDLG
IVGDCTAVLRDMVARWKERHLACDAAVLQKWWQEIDHWRARKSLAYRASNKVIKPQYAIQRLFELTRGRDTYISTEVGQH
QMWAAQHYHFDEPNRWMTSGGLGTMGYGLPAAIGAQMAHPGSLVVDIAGEASILMNIQELSTAVQFRLPVKIFILNNQYM
GMVRQWQELLHGGRYSESYSEALPDFVKLAEAYGCHGIRCADPNTLDAAILEMIETPKPVLFDCIVDREENCLPMIPSGK
AHNEMILPDMETELGTAIDAAGKILV

Sequences:

>Translated_586_residues
MSEKMTGAQMVVTALKDQGVDTIFGYPGGAVLPIYDALFHQNSVKHVLVRHEQGAVHAAEGYARSSGKIGVVLVTSGPGA
TNAVTGLTDALLDSIPLVCITGQVPTHLIGSDAFQECDTVGITRHCTKHNYLVRQVEDLPRILHEAFYVAQHGRPGPVVI
DIPKDVQFALGLYAGPENIEHKTYKPAFKGDLGRIDEALSWIAAAKRPIFYTGGGIINSGPEASALLRELVDLTGAPITS
TLMGLGAYPASSPRWLGMLGMHGTYEANNAMHDCDLMIAIGARFDDRITGRIDAFSPGSRKIHIDIDPSSINKTVKVDLG
IVGDCTAVLRDMVARWKERHLACDAAVLQKWWQEIDHWRARKSLAYRASNKVIKPQYAIQRLFELTRGRDTYISTEVGQH
QMWAAQHYHFDEPNRWMTSGGLGTMGYGLPAAIGAQMAHPGSLVVDIAGEASILMNIQELSTAVQFRLPVKIFILNNQYM
GMVRQWQELLHGGRYSESYSEALPDFVKLAEAYGCHGIRCADPNTLDAAILEMIETPKPVLFDCIVDREENCLPMIPSGK
AHNEMILPDMETELGTAIDAAGKILV
>Mature_585_residues
SEKMTGAQMVVTALKDQGVDTIFGYPGGAVLPIYDALFHQNSVKHVLVRHEQGAVHAAEGYARSSGKIGVVLVTSGPGAT
NAVTGLTDALLDSIPLVCITGQVPTHLIGSDAFQECDTVGITRHCTKHNYLVRQVEDLPRILHEAFYVAQHGRPGPVVID
IPKDVQFALGLYAGPENIEHKTYKPAFKGDLGRIDEALSWIAAAKRPIFYTGGGIINSGPEASALLRELVDLTGAPITST
LMGLGAYPASSPRWLGMLGMHGTYEANNAMHDCDLMIAIGARFDDRITGRIDAFSPGSRKIHIDIDPSSINKTVKVDLGI
VGDCTAVLRDMVARWKERHLACDAAVLQKWWQEIDHWRARKSLAYRASNKVIKPQYAIQRLFELTRGRDTYISTEVGQHQ
MWAAQHYHFDEPNRWMTSGGLGTMGYGLPAAIGAQMAHPGSLVVDIAGEASILMNIQELSTAVQFRLPVKIFILNNQYMG
MVRQWQELLHGGRYSESYSEALPDFVKLAEAYGCHGIRCADPNTLDAAILEMIETPKPVLFDCIVDREENCLPMIPSGKA
HNEMILPDMETELGTAIDAAGKILV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=25, Blast_Score=159, Evalue=6e-39,
Organism=Homo sapiens, GI21361361, Length=557, Percent_Identity=24.4165170556553, Blast_Score=135, Evalue=2e-31,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=51.3937282229965, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=43.4094903339192, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1786717, Length=567, Percent_Identity=34.5679012345679, Blast_Score=322, Evalue=4e-89,
Organism=Escherichia coli, GI1787096, Length=560, Percent_Identity=30, Blast_Score=209, Evalue=6e-55,
Organism=Escherichia coli, GI1788716, Length=580, Percent_Identity=25.1724137931034, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=580, Percent_Identity=25.3448275862069, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17531301, Length=580, Percent_Identity=25.3448275862069, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17542570, Length=515, Percent_Identity=27.1844660194175, Blast_Score=134, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6323755, Length=592, Percent_Identity=46.9594594594595, Blast_Score=518, Evalue=1e-147,
Organism=Saccharomyces cerevisiae, GI6320816, Length=489, Percent_Identity=25.3578732106339, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321524, Length=598, Percent_Identity=22.2408026755853, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6323073, Length=580, Percent_Identity=22.4137931034483, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323163, Length=585, Percent_Identity=22.5641025641026, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19922626, Length=548, Percent_Identity=24.6350364963504, Blast_Score=145, Evalue=8e-35,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64075; Mature: 63944

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKMTGAQMVVTALKDQGVDTIFGYPGGAVLPIYDALFHQNSVKHVLVRHEQGAVHAAE
CCCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEHHC
GYARSSGKIGVVLVTSGPGATNAVTGLTDALLDSIPLVCITGQVPTHLIGSDAFQECDTV
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHHHC
GITRHCTKHNYLVRQVEDLPRILHEAFYVAQHGRPGPVVIDIPKDVQFALGLYAGPENIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHEECCCCCCC
HKTYKPAFKGDLGRIDEALSWIAAAKRPIFYTGGGIINSGPEASALLRELVDLTGAPITS
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHH
TLMGLGAYPASSPRWLGMLGMHGTYEANNAMHDCDLMIAIGARFDDRITGRIDAFSPGSR
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCC
KIHIDIDPSSINKTVKVDLGIVGDCTAVLRDMVARWKERHLACDAAVLQKWWQEIDHWRA
EEEEEECHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSLAYRASNKVIKPQYAIQRLFELTRGRDTYISTEVGQHQMWAAQHYHFDEPNRWMTSG
HHHHHHHHCCCEECHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHCCCCCCCCCEEECC
GLGTMGYGLPAAIGAQMAHPGSLVVDIAGEASILMNIQELSTAVQFRLPVKIFILNNQYM
CCCCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHHEEECCEEEEEECCHHH
GMVRQWQELLHGGRYSESYSEALPDFVKLAEAYGCHGIRCADPNTLDAAILEMIETPKPV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCE
LFDCIVDREENCLPMIPSGKAHNEMILPDMETELGTAIDAAGKILV
EEHEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SEKMTGAQMVVTALKDQGVDTIFGYPGGAVLPIYDALFHQNSVKHVLVRHEQGAVHAAE
CCCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHCCCCCCEEEEECCCCCEEHHC
GYARSSGKIGVVLVTSGPGATNAVTGLTDALLDSIPLVCITGQVPTHLIGSDAFQECDTV
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHHHC
GITRHCTKHNYLVRQVEDLPRILHEAFYVAQHGRPGPVVIDIPKDVQFALGLYAGPENIE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHEECCCCCCC
HKTYKPAFKGDLGRIDEALSWIAAAKRPIFYTGGGIINSGPEASALLRELVDLTGAPITS
CCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCHHH
TLMGLGAYPASSPRWLGMLGMHGTYEANNAMHDCDLMIAIGARFDDRITGRIDAFSPGSR
HHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCC
KIHIDIDPSSINKTVKVDLGIVGDCTAVLRDMVARWKERHLACDAAVLQKWWQEIDHWRA
EEEEEECHHHCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RKSLAYRASNKVIKPQYAIQRLFELTRGRDTYISTEVGQHQMWAAQHYHFDEPNRWMTSG
HHHHHHHHCCCEECHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHCCCCCCCCCEEECC
GLGTMGYGLPAAIGAQMAHPGSLVVDIAGEASILMNIQELSTAVQFRLPVKIFILNNQYM
CCCCCCCCCHHHHHHHHCCCCCEEEEECCCCEEEEEHHHHHHHHEEECCEEEEEECCHHH
GMVRQWQELLHGGRYSESYSEALPDFVKLAEAYGCHGIRCADPNTLDAAILEMIETPKPV
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCCE
LFDCIVDREENCLPMIPSGKAHNEMILPDMETELGTAIDAAGKILV
EEHEEECCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]