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The map label for this gene is thrS [H]

Identifier: 182679322

GI number: 182679322

Start: 2706704

End: 2708638

Strand: Reverse

Name: thrS [H]

Synonym: Bind_2368

Alternate gene names: 182679322

Gene position: 2708638-2706704 (Counterclockwise)

Preceding gene: 182679323

Following gene: 182679321

Centisome position: 64.95

GC content: 57.47

Gene sequence:

>1935_bases
GTGATTTCCGTGACTTTCCCCGATGGCGCGGCGCGCCAGTTCGAGCCGGGCGTCAGTGGCGTCGACATTGCCAAAAGCAT
TTCGCCCTCGCTCGCGAAAAAAGCACTCGCCATGTCGCTCAATGGGACGTTGAGTGATCTTGCCGACAAGGTTGATCGCG
ACGCCCGCATCGAATTCATTACGCGTGATGATCCGCGCGGGCTTGATCTCATCCGTCACGATGCCGCCCATGTGATGGCG
GAGGCGGTGCAGACGCTTTATCCCGGCACGCAAGTCACAATCGGTCCGGTGATCGAAAATGGCTTTTATTACGATTTCTA
CCGCAATGAGCCGTTTACGCCGGCCGACTTCGCAGCCATCGAGAAAAAGATGGGCGAGATCATCGCCCGCGACAAGCCCT
TCACCAAGGAGGTGTGGTCGCGTGACGAGGCCAAGGCCTGGTTCGAGACGCATGGTGAGGCTTTCAAGGTCGAACTCGTC
GATTCAATCCCTGGCAACGAGGATCTGAAGATCTATCGCCAGGGCGAATGGCTCGACCTTTGTCGTGGTCCGCATCTCCC
CTCAACGGGCAAGATTGGCACGGCCTTCAAGCTCATGAAGGTGGCGGGCGCCTATTGGCGTGGTGATCATACCAAGCCGA
TGCTGCAACGCATCTATGCGACGGCCTTCGCGAATAAACAGGAACTCGACGCTTATTTGAAACAGCTCGAGGAGGCCGAA
AAGCGCGATCATCGCCGCCTGGGCCGCGAGATGGATCTGTTCCATTTCCAGGAAGAAGGGCCGGGCACGGTTTTCTGGCA
TCCCAAGGGCTGGACCTTGTTCCAGACCTTGATTTCCTACATGCGCCGCCGCCAGCAGGAGGCCGGTTATGTCGAGGTCA
ATACGCCGCAAGTGCTTGACCGGGCCTTGTGGGAGACCTCCGGCCATTGGTCGACCTATCGCGAAAATATGTTCATCACC
ACCACGGAGGATGAGCGTGTCTTCGCGCTGAAGCCGATGAATTGTCCGGGCCATGTGCAGATTTTCAAGAATGGCCTCAA
ATCCTATCGCGACTTGCCTTTGAAGATCGCTGAATTCGGTGTCGTGCATCGTTATGAGCCTTCGGGCGCTTTGCACGGGG
TGATGCGTGTGCGCGCCTTCACGCAGGATGACGCGCATATTTTCTGCACCGAAGACCAGATCATGGACGAGGTGTTGAAG
ATCAACGACCTCATCCTGTCGATCTACCGCGATTTCGGCTTCGAGGATATTGTCATCAAACTCTCGACGCGGCCGGAAAA
ACGTGTCGGTTCGGACGAGGCCTGGGACAAGGCGGAAGCCGCGCTGACACGGGTGCTCGAAAAGCTGGGTAATCAGTTCA
AGACGGGCATCAATCCGGGTGAGGGGGCGTTCTACGGACCGAAACTCGAATATACATTGCGCGATGCCATTGGCCGGGAA
TGGCAATGCGGCACGACGCAGGTCGATTTCAATCTGCCGGGACGATTCGGCGCGTTCTATATCGCGCCCGAGGGTGACAA
GACCGTACCGGTAATGATCCATCGCGCCATGTTCGGTTCGCTCGAGCGTTTCACCGGCATTCTCATCGAGCACTATGCCG
GTCATCTGCCTTTATGGCTCTCGCCTCTGCAGATCGTCGTCGCGACGATCACGCAGGAAGGCGATGATTATGCTTATGAA
GTGATGGCTGCGGCGCGCAAGCGCGGGCTCCGTGTCGATGGTGACCTGCGCAATGAAAAGATCACCTATAAGGTACGCGA
ACATTCGCTTGCCAAAGTGCCGGTGCTCTTTGTCCTCGGCAAGAAGGAAGCAGCCGAGCGCACGGTGTCAATCCGCAGGC
TTGGTTCCAATGATCAGATAAGCCTGGGGCTCGAACAAGCGCTCGAATTGCTTGCCGCCGAAGCGACGCCTCCCGATTTG
CGGCCGGCGTCCTGA

Upstream 100 bases:

>100_bases
TAGAGGCCGCAGATCGATTCCAAAAGGCAGTTCGATTGGATAAGATACGCGAAGCTTACGCCGATTGGTTCTGGCGAGTG
AGCAACCAAGGAGAACAATT

Downstream 100 bases:

>100_bases
AGCAATGTCCTGAATTGAGCCTCCAAGAGGGTATGCCCTCTTGGAGGGAGGACTTTTATTTTTTCTATTTCAAGAAATTA
TCATGGAACAGCGCGTCACC

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MISVTFPDGAARQFEPGVSGVDIAKSISPSLAKKALAMSLNGTLSDLADKVDRDARIEFITRDDPRGLDLIRHDAAHVMA
EAVQTLYPGTQVTIGPVIENGFYYDFYRNEPFTPADFAAIEKKMGEIIARDKPFTKEVWSRDEAKAWFETHGEAFKVELV
DSIPGNEDLKIYRQGEWLDLCRGPHLPSTGKIGTAFKLMKVAGAYWRGDHTKPMLQRIYATAFANKQELDAYLKQLEEAE
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQTLISYMRRRQQEAGYVEVNTPQVLDRALWETSGHWSTYRENMFIT
TTEDERVFALKPMNCPGHVQIFKNGLKSYRDLPLKIAEFGVVHRYEPSGALHGVMRVRAFTQDDAHIFCTEDQIMDEVLK
INDLILSIYRDFGFEDIVIKLSTRPEKRVGSDEAWDKAEAALTRVLEKLGNQFKTGINPGEGAFYGPKLEYTLRDAIGRE
WQCGTTQVDFNLPGRFGAFYIAPEGDKTVPVMIHRAMFGSLERFTGILIEHYAGHLPLWLSPLQIVVATITQEGDDYAYE
VMAAARKRGLRVDGDLRNEKITYKVREHSLAKVPVLFVLGKKEAAERTVSIRRLGSNDQISLGLEQALELLAAEATPPDL
RPAS

Sequences:

>Translated_644_residues
MISVTFPDGAARQFEPGVSGVDIAKSISPSLAKKALAMSLNGTLSDLADKVDRDARIEFITRDDPRGLDLIRHDAAHVMA
EAVQTLYPGTQVTIGPVIENGFYYDFYRNEPFTPADFAAIEKKMGEIIARDKPFTKEVWSRDEAKAWFETHGEAFKVELV
DSIPGNEDLKIYRQGEWLDLCRGPHLPSTGKIGTAFKLMKVAGAYWRGDHTKPMLQRIYATAFANKQELDAYLKQLEEAE
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQTLISYMRRRQQEAGYVEVNTPQVLDRALWETSGHWSTYRENMFIT
TTEDERVFALKPMNCPGHVQIFKNGLKSYRDLPLKIAEFGVVHRYEPSGALHGVMRVRAFTQDDAHIFCTEDQIMDEVLK
INDLILSIYRDFGFEDIVIKLSTRPEKRVGSDEAWDKAEAALTRVLEKLGNQFKTGINPGEGAFYGPKLEYTLRDAIGRE
WQCGTTQVDFNLPGRFGAFYIAPEGDKTVPVMIHRAMFGSLERFTGILIEHYAGHLPLWLSPLQIVVATITQEGDDYAYE
VMAAARKRGLRVDGDLRNEKITYKVREHSLAKVPVLFVLGKKEAAERTVSIRRLGSNDQISLGLEQALELLAAEATPPDL
RPAS
>Mature_644_residues
MISVTFPDGAARQFEPGVSGVDIAKSISPSLAKKALAMSLNGTLSDLADKVDRDARIEFITRDDPRGLDLIRHDAAHVMA
EAVQTLYPGTQVTIGPVIENGFYYDFYRNEPFTPADFAAIEKKMGEIIARDKPFTKEVWSRDEAKAWFETHGEAFKVELV
DSIPGNEDLKIYRQGEWLDLCRGPHLPSTGKIGTAFKLMKVAGAYWRGDHTKPMLQRIYATAFANKQELDAYLKQLEEAE
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQTLISYMRRRQQEAGYVEVNTPQVLDRALWETSGHWSTYRENMFIT
TTEDERVFALKPMNCPGHVQIFKNGLKSYRDLPLKIAEFGVVHRYEPSGALHGVMRVRAFTQDDAHIFCTEDQIMDEVLK
INDLILSIYRDFGFEDIVIKLSTRPEKRVGSDEAWDKAEAALTRVLEKLGNQFKTGINPGEGAFYGPKLEYTLRDAIGRE
WQCGTTQVDFNLPGRFGAFYIAPEGDKTVPVMIHRAMFGSLERFTGILIEHYAGHLPLWLSPLQIVVATITQEGDDYAYE
VMAAARKRGLRVDGDLRNEKITYKVREHSLAKVPVLFVLGKKEAAERTVSIRRLGSNDQISLGLEQALELLAAEATPPDL
RPAS

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=615, Percent_Identity=40.8130081300813, Blast_Score=464, Evalue=1e-130,
Organism=Homo sapiens, GI38570111, Length=616, Percent_Identity=39.1233766233766, Blast_Score=454, Evalue=1e-127,
Organism=Homo sapiens, GI20070344, Length=620, Percent_Identity=37.741935483871, Blast_Score=417, Evalue=1e-116,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=49.8442367601246, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=136, Percent_Identity=30.8823529411765, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71984192, Length=619, Percent_Identity=40.064620355412, Blast_Score=467, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI71984184, Length=619, Percent_Identity=40.2261712439418, Blast_Score=467, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322112, Length=631, Percent_Identity=40.7290015847861, Blast_Score=481, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322654, Length=397, Percent_Identity=37.0277078085642, Blast_Score=270, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24583842, Length=616, Percent_Identity=39.9350649350649, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI28574141, Length=616, Percent_Identity=39.9350649350649, Blast_Score=466, Evalue=1e-131,
Organism=Drosophila melanogaster, GI24583839, Length=616, Percent_Identity=39.9350649350649, Blast_Score=466, Evalue=1e-131,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 72912; Mature: 72912

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISVTFPDGAARQFEPGVSGVDIAKSISPSLAKKALAMSLNGTLSDLADKVDRDARIEFI
CEEEECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEE
TRDDPRGLDLIRHDAAHVMAEAVQTLYPGTQVTIGPVIENGFYYDFYRNEPFTPADFAAI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHH
EKKMGEIIARDKPFTKEVWSRDEAKAWFETHGEAFKVELVDSIPGNEDLKIYRQGEWLDL
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCCHHH
CRGPHLPSTGKIGTAFKLMKVAGAYWRGDHTKPMLQRIYATAFANKQELDAYLKQLEEAE
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQTLISYMRRRQQEAGYVEVNTPQVLD
HHHHHHHCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHH
RALWETSGHWSTYRENMFITTTEDERVFALKPMNCPGHVQIFKNGLKSYRDLPLKIAEFG
HHHHHCCCCCHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHCC
VVHRYEPSGALHGVMRVRAFTQDDAHIFCTEDQIMDEVLKINDLILSIYRDFGFEDIVIK
EEEEECCCHHHHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LSTRPEKRVGSDEAWDKAEAALTRVLEKLGNQFKTGINPGEGAFYGPKLEYTLRDAIGRE
ECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHCCCC
WQCGTTQVDFNLPGRFGAFYIAPEGDKTVPVMIHRAMFGSLERFTGILIEHYAGHLPLWL
CCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SPLQIVVATITQEGDDYAYEVMAAARKRGLRVDGDLRNEKITYKVREHSLAKVPVLFVLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEC
KKEAAERTVSIRRLGSNDQISLGLEQALELLAAEATPPDLRPAS
CHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MISVTFPDGAARQFEPGVSGVDIAKSISPSLAKKALAMSLNGTLSDLADKVDRDARIEFI
CEEEECCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEE
TRDDPRGLDLIRHDAAHVMAEAVQTLYPGTQVTIGPVIENGFYYDFYRNEPFTPADFAAI
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCHHHHHH
EKKMGEIIARDKPFTKEVWSRDEAKAWFETHGEAFKVELVDSIPGNEDLKIYRQGEWLDL
HHHHHHHHHCCCCCHHHHHCCHHHHHHHHCCCCEEEEEEEECCCCCCCEEEEECCCCHHH
CRGPHLPSTGKIGTAFKLMKVAGAYWRGDHTKPMLQRIYATAFANKQELDAYLKQLEEAE
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRDHRRLGREMDLFHFQEEGPGTVFWHPKGWTLFQTLISYMRRRQQEAGYVEVNTPQVLD
HHHHHHHCCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHH
RALWETSGHWSTYRENMFITTTEDERVFALKPMNCPGHVQIFKNGLKSYRDLPLKIAEFG
HHHHHCCCCCHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHCC
VVHRYEPSGALHGVMRVRAFTQDDAHIFCTEDQIMDEVLKINDLILSIYRDFGFEDIVIK
EEEEECCCHHHHHHHHHHEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
LSTRPEKRVGSDEAWDKAEAALTRVLEKLGNQFKTGINPGEGAFYGPKLEYTLRDAIGRE
ECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHCCCC
WQCGTTQVDFNLPGRFGAFYIAPEGDKTVPVMIHRAMFGSLERFTGILIEHYAGHLPLWL
CCCCCEEEEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SPLQIVVATITQEGDDYAYEVMAAARKRGLRVDGDLRNEKITYKVREHSLAKVPVLFVLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCEEEEEC
KKEAAERTVSIRRLGSNDQISLGLEQALELLAAEATPPDLRPAS
CHHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA