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The map label for this gene is cysT [H]

Identifier: 182679219

GI number: 182679219

Start: 2594219

End: 2595049

Strand: Reverse

Name: cysT [H]

Synonym: Bind_2259

Alternate gene names: 182679219

Gene position: 2595049-2594219 (Counterclockwise)

Preceding gene: 182679225

Following gene: 182679218

Centisome position: 62.23

GC content: 59.81

Gene sequence:

>831_bases
TTGTCCTTTCGGCGTCCATCCATTCTGCCTGGTTTCGGCCTGACGCTTGGCTATACCCTCGTCTATCTTGCCGTCATTGT
TCTGTTCCCGCTGGCGCTCCTCATCCTTCGGGCAAGCAGTCTCGGGATCTGGGGTTTTTGGCAGATCGCCACGGAGCCGC
GCGTGCTCGCGGCCCTAAGGACCAGTTTCGGCATTGCCTTGGCCGCAGCGGCCATCAATGTCGTTTTTGGCGGCATCATC
GCCTGGGTTCTCGCCCGCTATGAATTTCCCGGACGACGCATCATCGATGCGATTGTCGATCTTCCCTTTGCCTTGCCGAC
GGCGGTCGCGGGTATCGCGCTCGCTTCGCTCTATGCGCCGAACGGCTGGGTTGGCGCCCTCGTCGCGCCGCTGGGTTGGC
GCATTGCCTTTACGCCGCTCGGCATCGGCATCGCTTTGATCTTTATCGGCTTGCCTTTCATCGTCCGGACGGTGCAGCCG
GTCGTGGAGGAGCTTGAAAGCGATGTCGAGGAGGCCTCGGCCACGCTCGGCGCGACACGCGTTCAGACGCTCTTCCGTGT
CATTCTGCCGGGTCTCATCCCGGCTCTACTGACCGGCTTTGCCTTGGCTTTCGCGCGGGGCGTCGGGGAATATGGGTCGG
TGATCTTTCTCGCCGGCAATATTCCTTTCGTTTCGGAAATCGCACCCCTGCTGATTGTCGTCAAACTCGAGCAATTCGAT
TATGCGGCGGCGACCGCCATCGCGACCTTGATGCTCATCGTGTCTTTTTCGATTCTCCTTGTCATCAATATCATCCAGGC
GTGGAGCCGGCGGAGGTTTGGTTATGTCTGA

Upstream 100 bases:

>100_bases
AAATACCGCGCAAAATATGTGAATTTGACTTGCCTCGTTTGCCCTCGTAGGATAATTTCCTATAGGGTATATAGACTTCA
GGGCCTATCAGGAGCCAGAT

Downstream 100 bases:

>100_bases
GACCCTGGTCGACTGGTCGCAGGAGGCCGGTTTCGCGCTTCCTGCCCAGCAAAAGGTCAAGCGCCGGGTTCGGACTCCCG
CGACGACGGAAGGCCCGTTT

Product: sulfate ABC transporter inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 275

Protein sequence:

>276_residues
MSFRRPSILPGFGLTLGYTLVYLAVIVLFPLALLILRASSLGIWGFWQIATEPRVLAALRTSFGIALAAAAINVVFGGII
AWVLARYEFPGRRIIDAIVDLPFALPTAVAGIALASLYAPNGWVGALVAPLGWRIAFTPLGIGIALIFIGLPFIVRTVQP
VVEELESDVEEASATLGATRVQTLFRVILPGLIPALLTGFALAFARGVGEYGSVIFLAGNIPFVSEIAPLLIVVKLEQFD
YAAATAIATLMLIVSFSILLVINIIQAWSRRRFGYV

Sequences:

>Translated_276_residues
MSFRRPSILPGFGLTLGYTLVYLAVIVLFPLALLILRASSLGIWGFWQIATEPRVLAALRTSFGIALAAAAINVVFGGII
AWVLARYEFPGRRIIDAIVDLPFALPTAVAGIALASLYAPNGWVGALVAPLGWRIAFTPLGIGIALIFIGLPFIVRTVQP
VVEELESDVEEASATLGATRVQTLFRVILPGLIPALLTGFALAFARGVGEYGSVIFLAGNIPFVSEIAPLLIVVKLEQFD
YAAATAIATLMLIVSFSILLVINIIQAWSRRRFGYV
>Mature_275_residues
SFRRPSILPGFGLTLGYTLVYLAVIVLFPLALLILRASSLGIWGFWQIATEPRVLAALRTSFGIALAAAAINVVFGGIIA
WVLARYEFPGRRIIDAIVDLPFALPTAVAGIALASLYAPNGWVGALVAPLGWRIAFTPLGIGIALIFIGLPFIVRTVQPV
VEELESDVEEASATLGATRVQTLFRVILPGLIPALLTGFALAFARGVGEYGSVIFLAGNIPFVSEIAPLLIVVKLEQFDY
AAATAIATLMLIVSFSILLVINIIQAWSRRRFGYV

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=276, Percent_Identity=47.463768115942, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI87082099, Length=245, Percent_Identity=34.2857142857143, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1786980, Length=231, Percent_Identity=30.3030303030303, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787369, Length=211, Percent_Identity=27.0142180094787, Blast_Score=71, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29566; Mature: 29435

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRRPSILPGFGLTLGYTLVYLAVIVLFPLALLILRASSLGIWGFWQIATEPRVLAALR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
TSFGIALAAAAINVVFGGIIAWVLARYEFPGRRIIDAIVDLPFALPTAVAGIALASLYAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
NGWVGALVAPLGWRIAFTPLGIGIALIFIGLPFIVRTVQPVVEELESDVEEASATLGATR
CCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQTLFRVILPGLIPALLTGFALAFARGVGEYGSVIFLAGNIPFVSEIAPLLIVVKLEQFD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
YAAATAIATLMLIVSFSILLVINIIQAWSRRRFGYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SFRRPSILPGFGLTLGYTLVYLAVIVLFPLALLILRASSLGIWGFWQIATEPRVLAALR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHH
TSFGIALAAAAINVVFGGIIAWVLARYEFPGRRIIDAIVDLPFALPTAVAGIALASLYAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
NGWVGALVAPLGWRIAFTPLGIGIALIFIGLPFIVRTVQPVVEELESDVEEASATLGATR
CCHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQTLFRVILPGLIPALLTGFALAFARGVGEYGSVIFLAGNIPFVSEIAPLLIVVKLEQFD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
YAAATAIATLMLIVSFSILLVINIIQAWSRRRFGYV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1708375 [H]