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The map label for this gene is cysW [H]

Identifier: 182679218

GI number: 182679218

Start: 2593306

End: 2594226

Strand: Reverse

Name: cysW [H]

Synonym: Bind_2258

Alternate gene names: 182679218

Gene position: 2594226-2593306 (Counterclockwise)

Preceding gene: 182679219

Following gene: 182679217

Centisome position: 62.21

GC content: 58.85

Gene sequence:

>921_bases
ATGTCTGAGACCCTGGTCGACTGGTCGCAGGAGGCCGGTTTCGCGCTTCCTGCCCAGCAAAAGGTCAAGCGCCGGGTTCG
GACTCCCGCGACGACGGAAGGCCCGTTTCTGACGGGGGCTCTCATTGCCATTGCCTTGGCTTTTTTGCTCTTCTTTCTGG
TGCTGCCGCTGATCGTCGTCTTCTACGAAGCGCTCAGTCATGGAATAGGCACTTATTGGTCTGCTCTTGCGGAGCCTGAC
GCGCGCGCCGCGATCGGACTGACGCTTTTCGTCGCGGCCATTGCCGTTCCGCTCAACGTCGTTTTCGGTCTCGCCGCCGC
CTGGGCCATCGCGAAATTCCAGTTTCCGGGCAAAAGTTTTCTGATCACCCTGATCGATCTGCCGTTCTCGGTCTCCCCCG
TGGTTGCCGGCCTCGTTTATGTGTTGGTGTTCGGGGCGCAGGGTCTGTTGGGACACTGGCTGCAAGCCAATGATATCAAA
ATCATCTTCGCCGTGCCCGGCGTCGTTCTGGCGACGATTTTCGTGACCTTTCCATTCGTTGCCCGGGAAATCATTCCGCT
GATGGAGACACAAGGCACGGTCGAGGAAGAAGCCGCGCTGACGCTCGGGGCGAGCGGTCTTGCGACCTTCTTCCGCATCA
CGCTTCCCAATATCAAATGGGCCTTGCTCTATGGCGTCCTGCTTTGCAATGCGCGGGCAATGGGCGAGTTCGGGGCTGTT
TCCGTGGTGTCGGGCCATATTCGCGGTTTGACCGATACCATGCCGCTTCACATAGAAATTCTGTATAATGAGTATAATAT
TGCGGCTGCTTTTGCCGTGGCGTCGCTGCTCGCGCTTCTGGCGCTCGTGACGCTCATCGCCAAGACCGTGCTCGAAAGCC
GGTTCGGCGACGAACTCGCCAGAGGTGCCCGCCGACACTAA

Upstream 100 bases:

>100_bases
GCGGCGGCGACCGCCATCGCGACCTTGATGCTCATCGTGTCTTTTTCGATTCTCCTTGTCATCAATATCATCCAGGCGTG
GAGCCGGCGGAGGTTTGGTT

Downstream 100 bases:

>100_bases
GCAAGCCAGATAGGGTTGCTTAGGGATCAATTGGATAGCTCTTTATCATCACCTCCGGCTTAGCGCCGGAACGCTGCTTT
CCCTCGCTTTTTGAAGAAGC

Product: sulfate ABC transporter inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSETLVDWSQEAGFALPAQQKVKRRVRTPATTEGPFLTGALIAIALAFLLFFLVLPLIVVFYEALSHGIGTYWSALAEPD
ARAAIGLTLFVAAIAVPLNVVFGLAAAWAIAKFQFPGKSFLITLIDLPFSVSPVVAGLVYVLVFGAQGLLGHWLQANDIK
IIFAVPGVVLATIFVTFPFVAREIIPLMETQGTVEEEAALTLGASGLATFFRITLPNIKWALLYGVLLCNARAMGEFGAV
SVVSGHIRGLTDTMPLHIEILYNEYNIAAAFAVASLLALLALVTLIAKTVLESRFGDELARGARRH

Sequences:

>Translated_306_residues
MSETLVDWSQEAGFALPAQQKVKRRVRTPATTEGPFLTGALIAIALAFLLFFLVLPLIVVFYEALSHGIGTYWSALAEPD
ARAAIGLTLFVAAIAVPLNVVFGLAAAWAIAKFQFPGKSFLITLIDLPFSVSPVVAGLVYVLVFGAQGLLGHWLQANDIK
IIFAVPGVVLATIFVTFPFVAREIIPLMETQGTVEEEAALTLGASGLATFFRITLPNIKWALLYGVLLCNARAMGEFGAV
SVVSGHIRGLTDTMPLHIEILYNEYNIAAAFAVASLLALLALVTLIAKTVLESRFGDELARGARRH
>Mature_305_residues
SETLVDWSQEAGFALPAQQKVKRRVRTPATTEGPFLTGALIAIALAFLLFFLVLPLIVVFYEALSHGIGTYWSALAEPDA
RAAIGLTLFVAAIAVPLNVVFGLAAAWAIAKFQFPGKSFLITLIDLPFSVSPVVAGLVYVLVFGAQGLLGHWLQANDIKI
IFAVPGVVLATIFVTFPFVAREIIPLMETQGTVEEEAALTLGASGLATFFRITLPNIKWALLYGVLLCNARAMGEFGAVS
VVSGHIRGLTDTMPLHIEILYNEYNIAAAFAVASLLALLALVTLIAKTVLESRFGDELARGARRH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=266, Percent_Identity=52.2556390977444, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1788764, Length=228, Percent_Identity=38.5964912280702, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786980, Length=181, Percent_Identity=35.9116022099448, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32824; Mature: 32693

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETLVDWSQEAGFALPAQQKVKRRVRTPATTEGPFLTGALIAIALAFLLFFLVLPLIVV
CCCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FYEALSHGIGTYWSALAEPDARAAIGLTLFVAAIAVPLNVVFGLAAAWAIAKFQFPGKSF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LITLIDLPFSVSPVVAGLVYVLVFGAQGLLGHWLQANDIKIIFAVPGVVLATIFVTFPFV
EEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHH
AREIIPLMETQGTVEEEAALTLGASGLATFFRITLPNIKWALLYGVLLCNARAMGEFGAV
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCHH
SVVSGHIRGLTDTMPLHIEILYNEYNIAAAFAVASLLALLALVTLIAKTVLESRFGDELA
HHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGARRH
HHCCCC
>Mature Secondary Structure 
SETLVDWSQEAGFALPAQQKVKRRVRTPATTEGPFLTGALIAIALAFLLFFLVLPLIVV
CCCCCCCHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
FYEALSHGIGTYWSALAEPDARAAIGLTLFVAAIAVPLNVVFGLAAAWAIAKFQFPGKSF
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
LITLIDLPFSVSPVVAGLVYVLVFGAQGLLGHWLQANDIKIIFAVPGVVLATIFVTFPFV
EEEEECCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHH
AREIIPLMETQGTVEEEAALTLGASGLATFFRITLPNIKWALLYGVLLCNARAMGEFGAV
HHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCHHCCCCCHH
SVVSGHIRGLTDTMPLHIEILYNEYNIAAAFAVASLLALLALVTLIAKTVLESRFGDELA
HHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGARRH
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]