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The map label for this gene is mutB [H]

Identifier: 182679161

GI number: 182679161

Start: 2513570

End: 2515753

Strand: Reverse

Name: mutB [H]

Synonym: Bind_2199

Alternate gene names: 182679161

Gene position: 2515753-2513570 (Counterclockwise)

Preceding gene: 182679162

Following gene: 182679160

Centisome position: 60.33

GC content: 58.75

Gene sequence:

>2184_bases
ATGAGCAGAATTCCCGATTTCGCCGCTCCGGACCAGGCGTCATGGGCCGATGTCCATGCTGCGACGCAGCCCGTGGGTAC
GTCGCCACGGATGACGGCTGAGGGCATTGCTATCAAAAGCCTCTATACAGAGACAGACCGGCACACGCTCGCCTCGGTCG
ATACTTATCCGGGAATGCGGCCGTTTCTGCGTGGTCCCTATCCGACCATGTATGTCACACAGCCTTGGACGATCCGCCAA
TATGCTGGTTTCTCCACGGCGGAAGAATCGAATGCTTTCTACCGCCGTAATCTCGCCGCCGGCCAGAAGGGCTTGTCCAT
CGCCTTCGATCTTGCGACGCATCGCGGCTATGATTCCGATCATCCGCGTGTTGCGAGCGATGTCGGCATGGCGGGCGTGG
CGATCGATTCGATCTATGACATGCGGACTTTGTTTCAGGGCATTCCCCTCGGTGACATCAGTGTCTCCATGACGATGAAT
GGCGCGGTTTTGCCGATTCTCGCGCTCTATATCGTCGCGGCCGAGGAACAGGGCGTGGCGCCCGAACGACTCGCGGGAAC
CATTCAGAACGATATTCTGAAAGAGTTCATGGTCCGCAATACCTACATTTATCCGCCCGAGGATTCGATGCGGATCGTCG
CGGATATTTTCGCTTTCACGGCGAAACATATGCCGAAGTTCAATTCGATCTCGATCTCCGGCTATCATATGCAGGAAGCC
GGCGCGACACAGGATCTGGAACTCGCCTATACACTCGCCGATGGCGTGGACTATATCCGCGCCGGCATCGCGGCGGGGCT
CGGCATTGATCAATTCGCTCCACGCCTGTCCTTCTTCTTCGCGACAGGCATGAATTTCTTCATGGAAATCGCCAAGCTGC
GGGCGGCGCGGCAGTTGTGGTGCGAGCTTGTCGAGCGGTTCGGCCCCAAGGATGAAAAATCCTTGATGCTGCGCACACAT
TGCCAGACTTCGGGCTGGTCGCTTGCTGCTCAGGATGTCTTCAACAATGTTGCGCGCACCTGCGTCGAGGCCATGGCCTC
GACAGCGGGGCAGACGCAATCCCTGCATACAAACGCGCTCGATGAAGCGCTTGCCTTGCCAAGCGCATTTTCGGCCCGTA
TCGCCCGCAACACCCAGCTTCTTTTGCAACAGGAAACCGGCATTACGCGGATCATCGATGTCTGGGGTGGTTCCTATTAT
GTCGAAGCCTTGACGGCGGAACTCGCCGCCAAGGCGCGTGGTCACATTGCCGAAATTGAGGCGCTTGGCGGCATGGCCAA
GGCAATTGGCAGCGGTTTGCCAAAATTGCGGATCGAACAGGCCGCCGCCCGCACACAGGGCCGGATAGACGCCGGGCTCG
ACCGCATTATCGGCGTCAATCATGTCAAGCCGATTGAGGAAAAGCCGATCGATATTTTGCAGATCGACAATTCGGCGGTG
CGTTCGGTTCAGATTGAAAAGCTCGCCCGGCTGCGTGCTGAACGTGACAATCAGGAAGTTTTGCGGGCGCTCGCAGCTCT
GACTGAGGCGGCGGCGGACCGAACCAAGGGGAATCTGCTGGCGTTGGCGGTTGCAGCAGCGCGCGCCAAGGCGACGGTTG
GGGAAATGTCCGCGGCGCTCGAAACAGTTTTCGGCCGCCATCGTGCCACGATTGAGGTCACGAGCGGTGTTTACAAAGAG
GCGATGAGCCACAAGGCGGAATCCATTCAGGAAGCCGTCACCGCTGTCGAGGATTTTACCCGAAACAGTGGCCATCTCCC
CGCCGTGCTCGTTGCGAAAATGGGCCAGGATGGCCATGACCGTGGGCAGAAAGTGGTGGCTTCCGGCTTTGCCGATCTCG
GCTTCAGGGTCGTGCTCGGTCCGCTCTTCGCCACGCCGGAGGAAACCGCGCGCCAGGCGATCGAGGAGGATGTCGATGTC
ATTGGCGTGTCGTCGCTCGCCGGTGGACATTTGAGCCTCGTGCCGGAATTGCGCGAGGAACTGGCCAAGCTTGGCCATCC
CGAAATCATGATTGTTGTCGGTGGCGTCGTGCCGCCGCAAGATTACGCACAATTGCGTGATTCCGGTGCCGCCGCGATCT
TTCCCCCGGGGACACCGCTTTTCTCGGCCGCTACGGAACTGCTTGAAACTCTCAGCGCGCATCAAGGTTTTGTCCAGAAG
GACCCAGCCTTTTATCTTCGTTAA

Upstream 100 bases:

>100_bases
GCGCCGGGATTGAGGCGTTCATCTTTACCGGCTGCGACGGGCTGGCCTATCCGACCGCTCTCCTGAAGGAAAAAGCATCT
ACTCTGGCGGAGGCGATCTA

Downstream 100 bases:

>100_bases
AAACAGCAAGAAGAGACAGCAAGAGCATGATCGGACGTTTGAACCATGTGGCGATCGCCGTGCCAGATCTCGCGGCGGCT
TCGGCGCTTTACACCCAGGC

Product: methylmalonyl-CoA mutase, large subunit

Products: NA

Alternate protein names: MCM-alpha [H]

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MSRIPDFAAPDQASWADVHAATQPVGTSPRMTAEGIAIKSLYTETDRHTLASVDTYPGMRPFLRGPYPTMYVTQPWTIRQ
YAGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVASDVGMAGVAIDSIYDMRTLFQGIPLGDISVSMTMN
GAVLPILALYIVAAEEQGVAPERLAGTIQNDILKEFMVRNTYIYPPEDSMRIVADIFAFTAKHMPKFNSISISGYHMQEA
GATQDLELAYTLADGVDYIRAGIAAGLGIDQFAPRLSFFFATGMNFFMEIAKLRAARQLWCELVERFGPKDEKSLMLRTH
CQTSGWSLAAQDVFNNVARTCVEAMASTAGQTQSLHTNALDEALALPSAFSARIARNTQLLLQQETGITRIIDVWGGSYY
VEALTAELAAKARGHIAEIEALGGMAKAIGSGLPKLRIEQAAARTQGRIDAGLDRIIGVNHVKPIEEKPIDILQIDNSAV
RSVQIEKLARLRAERDNQEVLRALAALTEAAADRTKGNLLALAVAAARAKATVGEMSAALETVFGRHRATIEVTSGVYKE
AMSHKAESIQEAVTAVEDFTRNSGHLPAVLVAKMGQDGHDRGQKVVASGFADLGFRVVLGPLFATPEETARQAIEEDVDV
IGVSSLAGGHLSLVPELREELAKLGHPEIMIVVGGVVPPQDYAQLRDSGAAAIFPPGTPLFSAATELLETLSAHQGFVQK
DPAFYLR

Sequences:

>Translated_727_residues
MSRIPDFAAPDQASWADVHAATQPVGTSPRMTAEGIAIKSLYTETDRHTLASVDTYPGMRPFLRGPYPTMYVTQPWTIRQ
YAGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVASDVGMAGVAIDSIYDMRTLFQGIPLGDISVSMTMN
GAVLPILALYIVAAEEQGVAPERLAGTIQNDILKEFMVRNTYIYPPEDSMRIVADIFAFTAKHMPKFNSISISGYHMQEA
GATQDLELAYTLADGVDYIRAGIAAGLGIDQFAPRLSFFFATGMNFFMEIAKLRAARQLWCELVERFGPKDEKSLMLRTH
CQTSGWSLAAQDVFNNVARTCVEAMASTAGQTQSLHTNALDEALALPSAFSARIARNTQLLLQQETGITRIIDVWGGSYY
VEALTAELAAKARGHIAEIEALGGMAKAIGSGLPKLRIEQAAARTQGRIDAGLDRIIGVNHVKPIEEKPIDILQIDNSAV
RSVQIEKLARLRAERDNQEVLRALAALTEAAADRTKGNLLALAVAAARAKATVGEMSAALETVFGRHRATIEVTSGVYKE
AMSHKAESIQEAVTAVEDFTRNSGHLPAVLVAKMGQDGHDRGQKVVASGFADLGFRVVLGPLFATPEETARQAIEEDVDV
IGVSSLAGGHLSLVPELREELAKLGHPEIMIVVGGVVPPQDYAQLRDSGAAAIFPPGTPLFSAATELLETLSAHQGFVQK
DPAFYLR
>Mature_726_residues
SRIPDFAAPDQASWADVHAATQPVGTSPRMTAEGIAIKSLYTETDRHTLASVDTYPGMRPFLRGPYPTMYVTQPWTIRQY
AGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSDHPRVASDVGMAGVAIDSIYDMRTLFQGIPLGDISVSMTMNG
AVLPILALYIVAAEEQGVAPERLAGTIQNDILKEFMVRNTYIYPPEDSMRIVADIFAFTAKHMPKFNSISISGYHMQEAG
ATQDLELAYTLADGVDYIRAGIAAGLGIDQFAPRLSFFFATGMNFFMEIAKLRAARQLWCELVERFGPKDEKSLMLRTHC
QTSGWSLAAQDVFNNVARTCVEAMASTAGQTQSLHTNALDEALALPSAFSARIARNTQLLLQQETGITRIIDVWGGSYYV
EALTAELAAKARGHIAEIEALGGMAKAIGSGLPKLRIEQAAARTQGRIDAGLDRIIGVNHVKPIEEKPIDILQIDNSAVR
SVQIEKLARLRAERDNQEVLRALAALTEAAADRTKGNLLALAVAAARAKATVGEMSAALETVFGRHRATIEVTSGVYKEA
MSHKAESIQEAVTAVEDFTRNSGHLPAVLVAKMGQDGHDRGQKVVASGFADLGFRVVLGPLFATPEETARQAIEEDVDVI
GVSSLAGGHLSLVPELREELAKLGHPEIMIVVGGVVPPQDYAQLRDSGAAAIFPPGTPLFSAATELLETLSAHQGFVQKD
PAFYLR

Specific function: Catalyzes the isomerization of succinyl-CoA to methylmalonyl-CoA during synthesis of propionate from tricarboxylic acid-cycle intermediates. This conversion most likely represents an important source of building blocks for polyketide antibiotic biosynthes

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=707, Percent_Identity=59.2644978783593, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1789284, Length=687, Percent_Identity=60.2620087336244, Blast_Score=820, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144500, Length=686, Percent_Identity=61.6618075801749, Blast_Score=868, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 78529; Mature: 78398

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIPDFAAPDQASWADVHAATQPVGTSPRMTAEGIAIKSLYTETDRHTLASVDTYPGMR
CCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHCCCCCCCCC
PFLRGPYPTMYVTQPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSD
HHHCCCCCEEEEECCEEHHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEEEHHCCCCCCC
HPRVASDVGMAGVAIDSIYDMRTLFQGIPLGDISVSMTMNGAVLPILALYIVAAEEQGVA
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCC
PERLAGTIQNDILKEFMVRNTYIYPPEDSMRIVADIFAFTAKHMPKFNSISISGYHMQEA
HHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECEEECCC
GATQDLELAYTLADGVDYIRAGIAAGLGIDQFAPRLSFFFATGMNFFMEIAKLRAARQLW
CCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CELVERFGPKDEKSLMLRTHCQTSGWSLAAQDVFNNVARTCVEAMASTAGQTQSLHTNAL
HHHHHHHCCCCCHHEHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DEALALPSAFSARIARNTQLLLQQETGITRIIDVWGGSYYVEALTAELAAKARGHIAEIE
HHHHHCCHHHHHHHHCCHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHH
ALGGMAKAIGSGLPKLRIEQAAARTQGRIDAGLDRIIGVNHVKPIEEKPIDILQIDNSAV
HHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHH
RSVQIEKLARLRAERDNQEVLRALAALTEAAADRTKGNLLALAVAAARAKATVGEMSAAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
ETVFGRHRATIEVTSGVYKEAMSHKAESIQEAVTAVEDFTRNSGHLPAVLVAKMGQDGHD
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHH
RGQKVVASGFADLGFRVVLGPLFATPEETARQAIEEDVDVIGVSSLAGGHLSLVPELREE
HHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEHHCCCCCCEECHHHHHH
LAKLGHPEIMIVVGGVVPPQDYAQLRDSGAAAIFPPGTPLFSAATELLETLSAHQGFVQK
HHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCC
DPAFYLR
CCCCCCC
>Mature Secondary Structure 
SRIPDFAAPDQASWADVHAATQPVGTSPRMTAEGIAIKSLYTETDRHTLASVDTYPGMR
CCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHCCCHHHHCCCCCCCCC
PFLRGPYPTMYVTQPWTIRQYAGFSTAEESNAFYRRNLAAGQKGLSIAFDLATHRGYDSD
HHHCCCCCEEEEECCEEHHHHCCCCCCHHCCHHHHHHHHCCCCCCEEEEEEHHCCCCCCC
HPRVASDVGMAGVAIDSIYDMRTLFQGIPLGDISVSMTMNGAVLPILALYIVAAEEQGVA
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCC
PERLAGTIQNDILKEFMVRNTYIYPPEDSMRIVADIFAFTAKHMPKFNSISISGYHMQEA
HHHHHHHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHCCCCCCEEEECEEECCC
GATQDLELAYTLADGVDYIRAGIAAGLGIDQFAPRLSFFFATGMNFFMEIAKLRAARQLW
CCCCCCEEEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CELVERFGPKDEKSLMLRTHCQTSGWSLAAQDVFNNVARTCVEAMASTAGQTQSLHTNAL
HHHHHHHCCCCCHHEHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
DEALALPSAFSARIARNTQLLLQQETGITRIIDVWGGSYYVEALTAELAAKARGHIAEIE
HHHHHCCHHHHHHHHCCHHHEEECCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHH
ALGGMAKAIGSGLPKLRIEQAAARTQGRIDAGLDRIIGVNHVKPIEEKPIDILQIDNSAV
HHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHH
RSVQIEKLARLRAERDNQEVLRALAALTEAAADRTKGNLLALAVAAARAKATVGEMSAAL
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHH
ETVFGRHRATIEVTSGVYKEAMSHKAESIQEAVTAVEDFTRNSGHLPAVLVAKMGQDGHD
HHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCHH
RGQKVVASGFADLGFRVVLGPLFATPEETARQAIEEDVDVIGVSSLAGGHLSLVPELREE
HHHHHHHHHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEHHCCCCCCEECHHHHHH
LAKLGHPEIMIVVGGVVPPQDYAQLRDSGAAAIFPPGTPLFSAATELLETLSAHQGFVQK
HHHCCCCEEEEEECCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCC
DPAFYLR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099072 [H]