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The map label for this gene is tig

Identifier: 182679148

GI number: 182679148

Start: 2497905

End: 2499287

Strand: Reverse

Name: tig

Synonym: Bind_2185

Alternate gene names: 182679148

Gene position: 2499287-2497905 (Counterclockwise)

Preceding gene: 182679152

Following gene: 182679147

Centisome position: 59.93

GC content: 56.4

Gene sequence:

>1383_bases
ATGCAAGTGACCGAAACCCTCTCGCAGGGTCTCAAGCGCGAATATAAAGTGGTCCTCCCGGCTGCCGACATCGCGTCCCG
CCTCGACGAGCAACTGCAAGAGATGAAAGCCAAGGTGCGGATTAATGGTTTTCGCCCTGGCAAGGTGCCGGTTGCCCATC
TGAAGCGTCTTTATGGCCGCTCCGTCATGCTCGACGTGGTGCAGGAGGCGGTCAACGAAGCCAATCGCAAGATTCTTGAA
GAGAATAACCTGCGCCCCGCCGGTGAGCCGCGAATCGATTTCGACGGCGAAAAGGAGGATATGGAGAAGGCGTTCGAATC
CACCGCCGATTTCGCCTTCAAGGTCGCCTTGGAAATCCTGCCGAAATTCGAGATTGGTGGTTTCGAGGATATTGCGATTG
AACGTTTCGTGGCGGATGTCCCCGAAGAGGAAGTCGAGCGGATTCTCGGCCGATTCGCCGATCAGAATCGCGCCTATGAA
CCGAGGGCTGAAGGTGCCGCCGCTGAAAAGGGCGACAAAGTAACGATTGATTTCGTTGGCAAGATCGACGGTGAGCCTTT
TGAAGGCGGCAGCGGCGAGGATATCGATCTTGTGCTTGGCTCCGAGAGCTTCATTCCGGGCTTCGAGGATCAGGTCATCG
GATTGAAGGTTGGCGAACAGCGCCAGATCGCGGTCACCTTCCCGGAAGATTATGCCGCCAAGCTTGCCGGCAAAGCTGCG
ACCTTCGACGTAACCGTGAAACAGGTCGCCGCGCCGGGTGAGCAGACGATCGACGACGAATTGGCCAAGGCCTTCGGCTA
TGAAACGCTCGAAGCCATGAAGGAGCAGATTCGCACGACGATTTCGGATGAATATGCCGCGGCCTCGCGCGCGCAATGGA
AGCGCGCTCTGCTCGACGAACTCGACAAGAAATATCAGTTCGAATTGCCGGAAGGTCTGGTCGAGAACGAATTCGCGACA
GTTTGGCGCAAAGTCGAACTCGATCAACAAAGAAGCGGCAAGACCTTCGAGGAAGAAGGAACGACCGAGGAAGCCGCGCG
CGCCGATTATCGCAAGATCGCCGAGCGTCGGGTCCGGCTGGGCCTGTTGCTGGCGGAAATTGGTGAAAAGGCCGGGATCG
TGGTTCCCGACGAGGAAGTCGGCAAGGCTCTGGTCGAGCGCGCACGCGCTTTTCCGGGTCAAGAAAAGATGATCTGGGAT
TTTTATCGCCAGAACCCGAAGGCTCTGAACGAAATTCGCGCGCCTTTGTTCGAAGACAAGACGGTCGATTATATTTTGAC
GCAGGCCAAGGTGACCGACACCAAGGTGTCGAAAGATGAATTGCTGAAAAAGGCGCAAGAGGCGGAAGAAAGCGAACCTG
CGGAAGAAGCGGTTTCCGAATAA

Upstream 100 bases:

>100_bases
GCATTGTGGTTTTCTCTGAAACGATAGGGCGGCGGCTCGTTGGCCATCGCGCGAATGGCCAATCTGGAACAAGGCTGAAA
GCCGAAAAGGGTTGATTGAG

Downstream 100 bases:

>100_bases
GGTTCAGAGGAACGCACGGTTCGCTCCCGCATTTTTAAGCATTTCGTACCCGAGGGTGATTCTTCACCCTCGGGTTCTTT
ATTGATGGGGCCCCGATCCT

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MQVTETLSQGLKREYKVVLPAADIASRLDEQLQEMKAKVRINGFRPGKVPVAHLKRLYGRSVMLDVVQEAVNEANRKILE
ENNLRPAGEPRIDFDGEKEDMEKAFESTADFAFKVALEILPKFEIGGFEDIAIERFVADVPEEEVERILGRFADQNRAYE
PRAEGAAAEKGDKVTIDFVGKIDGEPFEGGSGEDIDLVLGSESFIPGFEDQVIGLKVGEQRQIAVTFPEDYAAKLAGKAA
TFDVTVKQVAAPGEQTIDDELAKAFGYETLEAMKEQIRTTISDEYAAASRAQWKRALLDELDKKYQFELPEGLVENEFAT
VWRKVELDQQRSGKTFEEEGTTEEAARADYRKIAERRVRLGLLLAEIGEKAGIVVPDEEVGKALVERARAFPGQEKMIWD
FYRQNPKALNEIRAPLFEDKTVDYILTQAKVTDTKVSKDELLKKAQEAEESEPAEEAVSE

Sequences:

>Translated_460_residues
MQVTETLSQGLKREYKVVLPAADIASRLDEQLQEMKAKVRINGFRPGKVPVAHLKRLYGRSVMLDVVQEAVNEANRKILE
ENNLRPAGEPRIDFDGEKEDMEKAFESTADFAFKVALEILPKFEIGGFEDIAIERFVADVPEEEVERILGRFADQNRAYE
PRAEGAAAEKGDKVTIDFVGKIDGEPFEGGSGEDIDLVLGSESFIPGFEDQVIGLKVGEQRQIAVTFPEDYAAKLAGKAA
TFDVTVKQVAAPGEQTIDDELAKAFGYETLEAMKEQIRTTISDEYAAASRAQWKRALLDELDKKYQFELPEGLVENEFAT
VWRKVELDQQRSGKTFEEEGTTEEAARADYRKIAERRVRLGLLLAEIGEKAGIVVPDEEVGKALVERARAFPGQEKMIWD
FYRQNPKALNEIRAPLFEDKTVDYILTQAKVTDTKVSKDELLKKAQEAEESEPAEEAVSE
>Mature_460_residues
MQVTETLSQGLKREYKVVLPAADIASRLDEQLQEMKAKVRINGFRPGKVPVAHLKRLYGRSVMLDVVQEAVNEANRKILE
ENNLRPAGEPRIDFDGEKEDMEKAFESTADFAFKVALEILPKFEIGGFEDIAIERFVADVPEEEVERILGRFADQNRAYE
PRAEGAAAEKGDKVTIDFVGKIDGEPFEGGSGEDIDLVLGSESFIPGFEDQVIGLKVGEQRQIAVTFPEDYAAKLAGKAA
TFDVTVKQVAAPGEQTIDDELAKAFGYETLEAMKEQIRTTISDEYAAASRAQWKRALLDELDKKYQFELPEGLVENEFAT
VWRKVELDQQRSGKTFEEEGTTEEAARADYRKIAERRVRLGLLLAEIGEKAGIVVPDEEVGKALVERARAFPGQEKMIWD
FYRQNPKALNEIRAPLFEDKTVDYILTQAKVTDTKVSKDELLKKAQEAEESEPAEEAVSE

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=447, Percent_Identity=32.2147651006711, Blast_Score=204, Evalue=1e-53,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_BEII9 (B2IGP4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833294.1
- GeneID:   6199270
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_2185
- HOGENOM:   HBG635105
- OMA:   FEGRIRN
- ProtClustDB:   PRK01490
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: NA

Molecular weight: Translated: 51547; Mature: 51547

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQVTETLSQGLKREYKVVLPAADIASRLDEQLQEMKAKVRINGFRPGKVPVAHLKRLYGR
CCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCC
SVMLDVVQEAVNEANRKILEENNLRPAGEPRIDFDGEKEDMEKAFESTADFAFKVALEIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PKFEIGGFEDIAIERFVADVPEEEVERILGRFADQNRAYEPRAEGAAAEKGDKVTIDFVG
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
KIDGEPFEGGSGEDIDLVLGSESFIPGFEDQVIGLKVGEQRQIAVTFPEDYAAKLAGKAA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHCCCCC
TFDVTVKQVAAPGEQTIDDELAKAFGYETLEAMKEQIRTTISDEYAAASRAQWKRALLDE
EEHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKKYQFELPEGLVENEFATVWRKVELDQQRSGKTFEEEGTTEEAARADYRKIAERRVRL
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
GLLLAEIGEKAGIVVPDEEVGKALVERARAFPGQEKMIWDFYRQNPKALNEIRAPLFEDK
HHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCC
TVDYILTQAKVTDTKVSKDELLKKAQEAEESEPAEEAVSE
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MQVTETLSQGLKREYKVVLPAADIASRLDEQLQEMKAKVRINGFRPGKVPVAHLKRLYGR
CCHHHHHHHHHHHHHEEEECHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHCC
SVMLDVVQEAVNEANRKILEENNLRPAGEPRIDFDGEKEDMEKAFESTADFAFKVALEIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
PKFEIGGFEDIAIERFVADVPEEEVERILGRFADQNRAYEPRAEGAAAEKGDKVTIDFVG
CCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEE
KIDGEPFEGGSGEDIDLVLGSESFIPGFEDQVIGLKVGEQRQIAVTFPEDYAAKLAGKAA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCEEEEECCHHHHHHHCCCCC
TFDVTVKQVAAPGEQTIDDELAKAFGYETLEAMKEQIRTTISDEYAAASRAQWKRALLDE
EEHHHHHHHHCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKKYQFELPEGLVENEFATVWRKVELDQQRSGKTFEEEGTTEEAARADYRKIAERRVRL
HHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHCCCHHHHHHHHHHHHHHHHHHH
GLLLAEIGEKAGIVVPDEEVGKALVERARAFPGQEKMIWDFYRQNPKALNEIRAPLFEDK
HHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHCCCCCCC
TVDYILTQAKVTDTKVSKDELLKKAQEAEESEPAEEAVSE
CHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA