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The map label for this gene is pheS

Identifier: 182679120

GI number: 182679120

Start: 2465024

End: 2466106

Strand: Reverse

Name: pheS

Synonym: Bind_2157

Alternate gene names: 182679120

Gene position: 2466106-2465024 (Counterclockwise)

Preceding gene: 182679121

Following gene: 182679112

Centisome position: 59.14

GC content: 56.42

Gene sequence:

>1083_bases
ATGTCTGATCTCGACACCCTACTGATGGAAACGCTGGCCCAGGTGGCCTTCGCCGCTGACGAGGGGGAGCTGGAAAACGT
CCGGGTCGGCGCGCTCGGCAAGAAAGGGACGATTTCGGCTCTTCTCGCCACTTTGGGCAAGATGAGCCCGGACGAACGCA
AGGTGCAAGGCGCTGCTATCAACGCGCTTAAGGACAAGGTCAATGAGGCGCTGGCTGAGCGCCGCCTCATCTTGAAAGAG
CAAAAGCTCGAGGCGCGGCTTGCAGCCGAAATCATTGATGTGACCTTGCCGGTGCTGCCGCGGCCGGAGGAATTGGGCCG
TATTCATCCGATCACCCAGGTCATGGATGAATTGGCCGCTATTTTCGCGGAAATGGGTTTCGCCATCGCCGAGGGGCCGG
ATATCGAAACCGACGATTATAATTTCACCAAGCTAAATTTCCCGCCGGACCATCCGGCGCGTGACATGCACGATACGTTT
TTCTTCGAGCCGGATGCGCAGGGACAGCGCAAGGTGCTGCGGACGCATACGAGTCCGGTGCAAGTGCGCACCATGCTCAC
GCAAAAACCGCCGATCCGCGTTATTTGTCCCGGCCGCACCTATCGCTGCGACTCGGATCAGACGCATACGCCGATGTTCC
ATCAGGTCGAAGGGCTGGTCATCGATAAGTCGGCCCATCTTGGCCATCTCAAATGGATCCTTGAAGAATTCTGCAAGGCT
TTCTTCGAGGTGCCGGATGTGAAGATGCGGTTTCGGCCGAGCTATTTCCCGTTCACAGAACCGTCGATGGAAGTTGATAT
CCAATGTTCACGCAAGGGGGGAGAAATCCGCTTCGGCGAGGGTGAGGATTGGCTGGAAATTTTGGGATGCGGGATGGTGC
ATCCCAACGTCTTGCGCAATTGCGGTCTGGACCCCGATGTCTATCAAGGCTTCGCCTGGGGCATGGGAATCGACCGTATC
GCCATGCTGAAATATGGCATGCCTGATCTCAGAGCCTTTTTTGAGGCTGATATTCGTTGGCTCAACCATTACGGATTCCG
ACCGCTCGATATGCCAAGCCTGCTTTCCGGATTGAGTAGCTAA

Upstream 100 bases:

>100_bases
GCGCTGAATCTACGCCCAGGCTCCTTCTCCGCCATGCACTCTCGACTTTGCCACAGGGGCGTGGCACAAGCGGCATAAAT
TTCGACCGGATTTCCAAACC

Downstream 100 bases:

>100_bases
TCGGCGTCGTTCATTGGCATGGACTCAATTCGTGTGGATGAGTCCAAAGGCATTCAAAATGTAATGCCTGCGTGAAGACC
CAAAACCAGCTCATTACCGA

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MSDLDTLLMETLAQVAFAADEGELENVRVGALGKKGTISALLATLGKMSPDERKVQGAAINALKDKVNEALAERRLILKE
QKLEARLAAEIIDVTLPVLPRPEELGRIHPITQVMDELAAIFAEMGFAIAEGPDIETDDYNFTKLNFPPDHPARDMHDTF
FFEPDAQGQRKVLRTHTSPVQVRTMLTQKPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKSAHLGHLKWILEEFCKA
FFEVPDVKMRFRPSYFPFTEPSMEVDIQCSRKGGEIRFGEGEDWLEILGCGMVHPNVLRNCGLDPDVYQGFAWGMGIDRI
AMLKYGMPDLRAFFEADIRWLNHYGFRPLDMPSLLSGLSS

Sequences:

>Translated_360_residues
MSDLDTLLMETLAQVAFAADEGELENVRVGALGKKGTISALLATLGKMSPDERKVQGAAINALKDKVNEALAERRLILKE
QKLEARLAAEIIDVTLPVLPRPEELGRIHPITQVMDELAAIFAEMGFAIAEGPDIETDDYNFTKLNFPPDHPARDMHDTF
FFEPDAQGQRKVLRTHTSPVQVRTMLTQKPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKSAHLGHLKWILEEFCKA
FFEVPDVKMRFRPSYFPFTEPSMEVDIQCSRKGGEIRFGEGEDWLEILGCGMVHPNVLRNCGLDPDVYQGFAWGMGIDRI
AMLKYGMPDLRAFFEADIRWLNHYGFRPLDMPSLLSGLSS
>Mature_359_residues
SDLDTLLMETLAQVAFAADEGELENVRVGALGKKGTISALLATLGKMSPDERKVQGAAINALKDKVNEALAERRLILKEQ
KLEARLAAEIIDVTLPVLPRPEELGRIHPITQVMDELAAIFAEMGFAIAEGPDIETDDYNFTKLNFPPDHPARDMHDTFF
FEPDAQGQRKVLRTHTSPVQVRTMLTQKPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKSAHLGHLKWILEEFCKAF
FEVPDVKMRFRPSYFPFTEPSMEVDIQCSRKGGEIRFGEGEDWLEILGCGMVHPNVLRNCGLDPDVYQGFAWGMGIDRIA
MLKYGMPDLRAFFEADIRWLNHYGFRPLDMPSLLSGLSS

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=32.962962962963, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI5729820, Length=265, Percent_Identity=27.5471698113208, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI1788007, Length=344, Percent_Identity=51.453488372093, Blast_Score=359, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32563657, Length=285, Percent_Identity=32.6315789473684, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17508957, Length=285, Percent_Identity=32.6315789473684, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32566635, Length=262, Percent_Identity=31.2977099236641, Blast_Score=109, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321087, Length=267, Percent_Identity=32.9588014981273, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=100, Percent_Identity=36, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI18858079, Length=275, Percent_Identity=30.9090909090909, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI17137424, Length=264, Percent_Identity=26.1363636363636, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_BEII9 (B2IGL6)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833266.1
- GeneID:   6200899
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_2157
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40561; Mature: 40430

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLDTLLMETLAQVAFAADEGELENVRVGALGKKGTISALLATLGKMSPDERKVQGAAI
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
NALKDKVNEALAERRLILKEQKLEARLAAEIIDVTLPVLPRPEELGRIHPITQVMDELAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
IFAEMGFAIAEGPDIETDDYNFTKLNFPPDHPARDMHDTFFFEPDAQGQRKVLRTHTSPV
HHHHHCCCEECCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCH
QVRTMLTQKPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKSAHLGHLKWILEEFCKA
HHHHHHCCCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHH
FFEVPDVKMRFRPSYFPFTEPSMEVDIQCSRKGGEIRFGEGEDWLEILGCGMVHPNVLRN
HHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHCCCCCHHHHHH
CGLDPDVYQGFAWGMGIDRIAMLKYGMPDLRAFFEADIRWLNHYGFRPLDMPSLLSGLSS
CCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
SDLDTLLMETLAQVAFAADEGELENVRVGALGKKGTISALLATLGKMSPDERKVQGAAI
CHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
NALKDKVNEALAERRLILKEQKLEARLAAEIIDVTLPVLPRPEELGRIHPITQVMDELAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHH
IFAEMGFAIAEGPDIETDDYNFTKLNFPPDHPARDMHDTFFFEPDAQGQRKVLRTHTSPV
HHHHHCCCEECCCCCCCCCCCEEEECCCCCCCCCCCCCCEEECCCCCHHHHHHHHCCCCH
QVRTMLTQKPPIRVICPGRTYRCDSDQTHTPMFHQVEGLVIDKSAHLGHLKWILEEFCKA
HHHHHHCCCCCEEEECCCCCEECCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHH
FFEVPDVKMRFRPSYFPFTEPSMEVDIQCSRKGGEIRFGEGEDWLEILGCGMVHPNVLRN
HHCCCCCEEEECCCCCCCCCCCCEEEEEECCCCCEEEECCCCHHHHHHHCCCCCHHHHHH
CGLDPDVYQGFAWGMGIDRIAMLKYGMPDLRAFFEADIRWLNHYGFRPLDMPSLLSGLSS
CCCCHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA