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The map label for this gene is kgtP [H]

Identifier: 182679090

GI number: 182679090

Start: 2428270

End: 2429604

Strand: Reverse

Name: kgtP [H]

Synonym: Bind_2127

Alternate gene names: 182679090

Gene position: 2429604-2428270 (Counterclockwise)

Preceding gene: 182679092

Following gene: 182679089

Centisome position: 58.26

GC content: 54.83

Gene sequence:

>1335_bases
ATGGTCGAGGTCACGCGGGATTATCACTTCTCACCGGCGGCTGATCCTTATGATCGCGCGGATGTACGACGGCGCACGAA
AGCCATTTTGATCGGTTCTGTTGGCAACCTGATAGAATGGTATGATGTCTACGCCTATTCGGCTTTTGCGCTTTATTTCG
GCGGTGCCTTTTTCCCGAGTGCCAATCTGGTTGCGCAGCAATTGGCGGCGGCCTCCTTGTTTGCCGCGGCTTTTCTCATG
CGTCCCATCGGCAGCCTGTTGTTTGGCTATTTAGCCGATCGTTATGGGCGGCGCATGGCGCTGACCTTTTCTATTCTTCT
CATGTGTTTCGGTTCCTTGCTGATTGCCGTGGCCCCGACTTATTCGACAATTGGCATCGGGGCACCGATCTTGTTGGCCT
TCGCTCGCATTCTGCAGGGGCTCAGCCAAGGCGGCGAATATGGCACAAGCGCCACTTATCTGGCGGAAGTTTCGCATCCG
GCGCGGCGCGGTTTTTATTCGGGCATCTGGTACACGACCTTAATTGGCGGCCAGCTCGCTGCGATCATCGTTCTGCTGAT
CTTGCAAAAATTGGTGCTCACGCCGGAGCAGCTTTATGCCTTTGGTTGGCGTATTCCGTTTTTTATCGGCGCCGCTCTCG
CGCTTTATTGTTTCTTCATGCGTCGTGACCTGCACGAGACCGATTTGTTCAAGAAATCGCAGGCGCTGGCCGAGAAAGTC
GGCACCTGGACGACCCTGCGCCGCAATTGGAAGACGATCCTTTATGTCATTGGCTTCACGGCGGGAGGCACCTCGGCCTT
TTACACCTACACGACCTATATGCAGAAATTCGTCAAATTGTCCGTGGGGCTCACCGAGGACGAGACGACGCTGATCATTT
TCGGCTCATCGACGCTCGCCGTTGCTCTTCAGCCGCTTTATGGCGCCTTGTCTGACAAGATCGGTCGTAAGCCCATGTTG
CTTGCCTTCGGTATCATGGGGACTTTGTTCACCTATCCGTTGCTCACGACGCTGCAGACGACGAAAAGCCCGCTCGTGGC
CTTCCTGTTGATCTGCGCTGGCTGGGGCATCATCACGCCCTATACGTCGACGGCGGTCATTGTGAAGGCGGAGCTTTTTC
CGACCTCCATCCGGGCGATTGGTGTCGGTTTTCCCTATGCCATTGCGGCTGCGGTCTTCGGCGGCACGATTGACAGCGTG
GCCTTGTCTTTCAAGAACCAAGGCCATGAAGAATGGTTCTTCCTCTATGCGACAGCCTGTATTTTCATGTCGCTGATCTT
TTATTGCTTCCTGCCGGATCTGAAGAAAAAATCCCGGATGGATGAGGCGGTTTAG

Upstream 100 bases:

>100_bases
GCCTCGGATGACTTAAGCGACCGACCTGGACAGGTAAGGATGAAAGATCCAGAGTTGCTTTCTGCCGGCAAGGAGACAGG
GCTGGGCATGGGGAGGAAAC

Downstream 100 bases:

>100_bases
CGTCTATATCGGGGTCGGTTTATGAGCGTCGGGCAATAAAGGATGCTTTCACCCTGGCCTCGATGCGTCTATGACGGAGG
TGGCCCGGTGCTCTCGCCCG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MVEVTRDYHFSPAADPYDRADVRRRTKAILIGSVGNLIEWYDVYAYSAFALYFGGAFFPSANLVAQQLAAASLFAAAFLM
RPIGSLLFGYLADRYGRRMALTFSILLMCFGSLLIAVAPTYSTIGIGAPILLAFARILQGLSQGGEYGTSATYLAEVSHP
ARRGFYSGIWYTTLIGGQLAAIIVLLILQKLVLTPEQLYAFGWRIPFFIGAALALYCFFMRRDLHETDLFKKSQALAEKV
GTWTTLRRNWKTILYVIGFTAGGTSAFYTYTTYMQKFVKLSVGLTEDETTLIIFGSSTLAVALQPLYGALSDKIGRKPML
LAFGIMGTLFTYPLLTTLQTTKSPLVAFLLICAGWGIITPYTSTAVIVKAELFPTSIRAIGVGFPYAIAAAVFGGTIDSV
ALSFKNQGHEEWFFLYATACIFMSLIFYCFLPDLKKKSRMDEAV

Sequences:

>Translated_444_residues
MVEVTRDYHFSPAADPYDRADVRRRTKAILIGSVGNLIEWYDVYAYSAFALYFGGAFFPSANLVAQQLAAASLFAAAFLM
RPIGSLLFGYLADRYGRRMALTFSILLMCFGSLLIAVAPTYSTIGIGAPILLAFARILQGLSQGGEYGTSATYLAEVSHP
ARRGFYSGIWYTTLIGGQLAAIIVLLILQKLVLTPEQLYAFGWRIPFFIGAALALYCFFMRRDLHETDLFKKSQALAEKV
GTWTTLRRNWKTILYVIGFTAGGTSAFYTYTTYMQKFVKLSVGLTEDETTLIIFGSSTLAVALQPLYGALSDKIGRKPML
LAFGIMGTLFTYPLLTTLQTTKSPLVAFLLICAGWGIITPYTSTAVIVKAELFPTSIRAIGVGFPYAIAAAVFGGTIDSV
ALSFKNQGHEEWFFLYATACIFMSLIFYCFLPDLKKKSRMDEAV
>Mature_444_residues
MVEVTRDYHFSPAADPYDRADVRRRTKAILIGSVGNLIEWYDVYAYSAFALYFGGAFFPSANLVAQQLAAASLFAAAFLM
RPIGSLLFGYLADRYGRRMALTFSILLMCFGSLLIAVAPTYSTIGIGAPILLAFARILQGLSQGGEYGTSATYLAEVSHP
ARRGFYSGIWYTTLIGGQLAAIIVLLILQKLVLTPEQLYAFGWRIPFFIGAALALYCFFMRRDLHETDLFKKSQALAEKV
GTWTTLRRNWKTILYVIGFTAGGTSAFYTYTTYMQKFVKLSVGLTEDETTLIIFGSSTLAVALQPLYGALSDKIGRKPML
LAFGIMGTLFTYPLLTTLQTTKSPLVAFLLICAGWGIITPYTSTAVIVKAELFPTSIRAIGVGFPYAIAAAVFGGTIDSV
ALSFKNQGHEEWFFLYATACIFMSLIFYCFLPDLKKKSRMDEAV

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=428, Percent_Identity=46.4953271028037, Blast_Score=346, Evalue=1e-96,
Organism=Escherichia coli, GI1790550, Length=392, Percent_Identity=33.1632653061224, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1788292, Length=388, Percent_Identity=29.3814432989691, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1789941, Length=386, Percent_Identity=29.5336787564767, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI87082231, Length=208, Percent_Identity=32.6923076923077, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082404, Length=343, Percent_Identity=23.9067055393586, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48923; Mature: 48923

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEVTRDYHFSPAADPYDRADVRRRTKAILIGSVGNLIEWYDVYAYSAFALYFGGAFFPS
CCEECCCCCCCCCCCCCHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCHHCCC
ANLVAQQLAAASLFAAAFLMRPIGSLLFGYLADRYGRRMALTFSILLMCFGSLLIAVAPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
YSTIGIGAPILLAFARILQGLSQGGEYGTSATYLAEVSHPARRGFYSGIWYTTLIGGQLA
CHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCHHHHHHHHHHHHHHHH
AIIVLLILQKLVLTPEQLYAFGWRIPFFIGAALALYCFFMRRDLHETDLFKKSQALAEKV
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GTWTTLRRNWKTILYVIGFTAGGTSAFYTYTTYMQKFVKLSVGLTEDETTLIIFGSSTLA
HHHHHHHHCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHH
VALQPLYGALSDKIGRKPMLLAFGIMGTLFTYPLLTTLQTTKSPLVAFLLICAGWGIITP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YTSTAVIVKAELFPTSIRAIGVGFPYAIAAAVFGGTIDSVALSFKNQGHEEWFFLYATAC
CCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
IFMSLIFYCFLPDLKKKSRMDEAV
HHHHHHHHHHCCCHHHHHHCCCCC
>Mature Secondary Structure
MVEVTRDYHFSPAADPYDRADVRRRTKAILIGSVGNLIEWYDVYAYSAFALYFGGAFFPS
CCEECCCCCCCCCCCCCHHHHHHHHHHEEEEECHHHHHHHHHHHHHHHHHHHHCCHHCCC
ANLVAQQLAAASLFAAAFLMRPIGSLLFGYLADRYGRRMALTFSILLMCFGSLLIAVAPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCC
YSTIGIGAPILLAFARILQGLSQGGEYGTSATYLAEVSHPARRGFYSGIWYTTLIGGQLA
CHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHCHHHHHHHHHHHHHHHH
AIIVLLILQKLVLTPEQLYAFGWRIPFFIGAALALYCFFMRRDLHETDLFKKSQALAEKV
HHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
GTWTTLRRNWKTILYVIGFTAGGTSAFYTYTTYMQKFVKLSVGLTEDETTLIIFGSSTLA
HHHHHHHHCHHEEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHH
VALQPLYGALSDKIGRKPMLLAFGIMGTLFTYPLLTTLQTTKSPLVAFLLICAGWGIITP
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YTSTAVIVKAELFPTSIRAIGVGFPYAIAAAVFGGTIDSVALSFKNQGHEEWFFLYATAC
CCCCEEEEEEECCCHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHH
IFMSLIFYCFLPDLKKKSRMDEAV
HHHHHHHHHHCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]