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The map label for this gene is glyS [H]

Identifier: 182679089

GI number: 182679089

Start: 2425664

End: 2428129

Strand: Reverse

Name: glyS [H]

Synonym: Bind_2126

Alternate gene names: 182679089

Gene position: 2428129-2425664 (Counterclockwise)

Preceding gene: 182679090

Following gene: 182679088

Centisome position: 58.23

GC content: 58.8

Gene sequence:

>2466_bases
ATGCCCGATCTTCTGCTTGAACTTTTCTCCGAGGAAATCCCCGCTCGAATGCAGGCGCAGGCCGCCGAGGATTTGAAGCG
GCTGGTGACCGGGGCTTTGAGCGAACAGGGATTGAGTTTCGAAGGTGCGGCGAGCTTTGCCACACCGCGGCGCCTGGCGC
TGCATGTCGCGGGCCTGCCCATCCGCCAAGCCGATGTGCGGGAGGAAAAGAAAGGCCCCAAAGTCGGCGCGCCGGAAGCC
GCGCTCCAAGGCTTTTTGAAAAGCGCTGGCCTCGCCTCACTTGATCAGGCCATTGTTCAGAAAGACGCCAAGAAAGGCGA
TTTCTATGTCGCGGTCATCGAGCGTGCAGGCCGTCCCGCACTCGATGTCCTGGCCGATATTTTGCCGGCCGTTATCAAAA
GCTTTCCCTGGCCTAAATCCATGCGCTGGGGCAGGGCCTCGGCGCGCTCCGATGCCTTGCGCTGGGTGCGGCCTTTGCAT
GCGATCCTCGCGACCTTTGGTCCCGAAACGGAAGAGCCGGATGTCGTGCCCTTTTCCGTCGAAGGGATCGAAGCGGGTCA
GACGACCTTCGGCCATCGCTTCCTGGCGCCCGAGCCCATCAAAATCCGCCGTTTCGACGATTATCTGCCGGCCCTTGAAA
AGGCCAAGGTGGTGCTCGATGCTGAGCGGAGGCGCGACATCATCTTGCATGACGCGCGTGATCTCAGCTTCGCGCAAGGC
CTGGATCTTATAGAAGACAAGGCTTTGTTGGAGGAGGTGGCGGGGCTTGTCGAATGGCCGGTGGTCCTTTTGGCGGAATT
CGATCCGCAATTTCTTGAAATCCCGCCGGAGGTTATCCGCGCGACGATTCGCGCCAATCAGAAATGTTTCGTGTTGAGGG
ATCCGGTCAGCGGCCGTCTCGCCCATAAATTCCTGCTCGTCGCAAATATCGAGGCGAGCGATGGCGGCAAGGCGATTGTC
GCGGGTAATGCACGCGTCGTGCGCGCCCGCCTGTCTGATGCCAAATTTTTCTGGGAGACGGATCTGCAAACACGGCTCGA
GGCGCGGCTACCCAAGCTCGAGACCATCGTGTTTCATGAAAAGCTCGGCACGCAGGGCGAACGCGTACAGCGCTTGGTCT
TGAACGCGCGTGATCTCAGCCCGATCGTTGGCGCGGATCAGGCCGAGGCGGCGCGGGCGGCGGAACTCGCCAAAACCGAT
CTTGTCTCGGAGATGGTTGGTGAATTTCCCGAATTGCAAGGCTTAATGGGCCGCTATTATGCGCAAAAGCAGGGGGAAAG
CTCCAATGTCGCGGCGGCGATTGAGGAGCATTACAAGCCGCAAGGGCCGAACGATCTCGTGCCGAACGCGCCAGTGTCCA
TTGCTGTAGCCCTTGCTGACAAGCTCGACAGCCTCGTGGGCTTTTTCGCCATTGACGAGCGCCCGAGCGGCAGCAAGGAT
CCCTATGCTTTGCGCCGCGCGGCGCTCGGTGTGATCGCGCTCATTCTTGAAAACAAGCTGCGCCTGCCGCTGCGTCGCCA
ATTGGAAAATGTTTTTGTGCATCTGGTCCGGGTCAGCGAGGCGGTTCCAGCCTTTAACTTGCTGACCCTTCGTGATGATC
GAGATGCCGATGCGGGCGAACTTGCCAGACTGGCGCAAGGCGGCCTTTGGGCTTGGGAATTCGCGCGCGAGCCGGCTAAA
AGCGCTCATGCCGTCGCATCACGCTATCTGGAGGCTCGTTTCGCCGGTGAAGGTGAAGAGCGAGAAGAGGATATGGCGTC
GTTCGCTGCCCTCCACGACATCGTCAAACCTTATGAGCGGCTGCGCGAAATCTGGCTCGCTTCGGGTCAGGCGGGGGCAG
AGGATCCAGATTTCATCGACATGCGGCAGGCCTTTTTGGCCGCTCGGGAGGATCTCATCGGGCAGCTCATCGCCTTTTTC
GGCGATCGTCTCAAAGTTTATCTGCGCGAGCGTGGTGCGCGCTATGATCTGATTGACGCGGTTTTTGCCTTGCCGGGCCA
GGATGATCTGCTCCTCATTGTCGCGCGCGTCGAGGCCTTGGCGAGTTTTCTTGATACAGAGGAAGGCAAGAATCTGCTCG
CCGGTTACAGGCGCGCGGCCAATATTTTGAAGGCTGAGGAAAAGAAAGATGGCGTTGGTGCTTTTAATGCGCCAGCCGAT
CCCGGCCTGTTACGCGAGGGACAAGAACGCGCCCTTTGGGAAAGAATCCGGCTGATGCGGGAAGAAGCCGAAACCCATTT
GTACAAAGCAGAGGAAGAAATCGGTCGGAAAACTTTTGAAGGCCCGACTCCCTTCGAAAAAGCCATGGAAAATTTGGCCT
CTTTGCGCGCGCCGGTCGATGCCTTTTTCGATCATGTGACAGTGAATGCGAGCGAGCCGGAACTTCGCGTGAACCGTCTG
CAATTGCTGAATGAACTTCGGCAGGCGGTGCATCGCATCGCGGATTTTTCCAAGATTGCGGGATAG

Upstream 100 bases:

>100_bases
GGATGCTTTCACCCTGGCCTCGATGCGTCTATGACGGAGGTGGCCCGGTGCTCTCGCCCGGGTCACGCCTCATCCCGCTT
CCAGCCAAGATAAATCCGCA

Downstream 100 bases:

>100_bases
AGCCTTAGCCAAAAAGGTCTCCAGCATCGCAGATGCTGGAGACACCGAAGCCCGTATCAATCTCCTTCATCGGCGAGCAT
TGCATCCCTTCGCGTCGAAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 821; Mature: 820

Protein sequence:

>821_residues
MPDLLLELFSEEIPARMQAQAAEDLKRLVTGALSEQGLSFEGAASFATPRRLALHVAGLPIRQADVREEKKGPKVGAPEA
ALQGFLKSAGLASLDQAIVQKDAKKGDFYVAVIERAGRPALDVLADILPAVIKSFPWPKSMRWGRASARSDALRWVRPLH
AILATFGPETEEPDVVPFSVEGIEAGQTTFGHRFLAPEPIKIRRFDDYLPALEKAKVVLDAERRRDIILHDARDLSFAQG
LDLIEDKALLEEVAGLVEWPVVLLAEFDPQFLEIPPEVIRATIRANQKCFVLRDPVSGRLAHKFLLVANIEASDGGKAIV
AGNARVVRARLSDAKFFWETDLQTRLEARLPKLETIVFHEKLGTQGERVQRLVLNARDLSPIVGADQAEAARAAELAKTD
LVSEMVGEFPELQGLMGRYYAQKQGESSNVAAAIEEHYKPQGPNDLVPNAPVSIAVALADKLDSLVGFFAIDERPSGSKD
PYALRRAALGVIALILENKLRLPLRRQLENVFVHLVRVSEAVPAFNLLTLRDDRDADAGELARLAQGGLWAWEFAREPAK
SAHAVASRYLEARFAGEGEEREEDMASFAALHDIVKPYERLREIWLASGQAGAEDPDFIDMRQAFLAAREDLIGQLIAFF
GDRLKVYLRERGARYDLIDAVFALPGQDDLLLIVARVEALASFLDTEEGKNLLAGYRRAANILKAEEKKDGVGAFNAPAD
PGLLREGQERALWERIRLMREEAETHLYKAEEEIGRKTFEGPTPFEKAMENLASLRAPVDAFFDHVTVNASEPELRVNRL
QLLNELRQAVHRIADFSKIAG

Sequences:

>Translated_821_residues
MPDLLLELFSEEIPARMQAQAAEDLKRLVTGALSEQGLSFEGAASFATPRRLALHVAGLPIRQADVREEKKGPKVGAPEA
ALQGFLKSAGLASLDQAIVQKDAKKGDFYVAVIERAGRPALDVLADILPAVIKSFPWPKSMRWGRASARSDALRWVRPLH
AILATFGPETEEPDVVPFSVEGIEAGQTTFGHRFLAPEPIKIRRFDDYLPALEKAKVVLDAERRRDIILHDARDLSFAQG
LDLIEDKALLEEVAGLVEWPVVLLAEFDPQFLEIPPEVIRATIRANQKCFVLRDPVSGRLAHKFLLVANIEASDGGKAIV
AGNARVVRARLSDAKFFWETDLQTRLEARLPKLETIVFHEKLGTQGERVQRLVLNARDLSPIVGADQAEAARAAELAKTD
LVSEMVGEFPELQGLMGRYYAQKQGESSNVAAAIEEHYKPQGPNDLVPNAPVSIAVALADKLDSLVGFFAIDERPSGSKD
PYALRRAALGVIALILENKLRLPLRRQLENVFVHLVRVSEAVPAFNLLTLRDDRDADAGELARLAQGGLWAWEFAREPAK
SAHAVASRYLEARFAGEGEEREEDMASFAALHDIVKPYERLREIWLASGQAGAEDPDFIDMRQAFLAAREDLIGQLIAFF
GDRLKVYLRERGARYDLIDAVFALPGQDDLLLIVARVEALASFLDTEEGKNLLAGYRRAANILKAEEKKDGVGAFNAPAD
PGLLREGQERALWERIRLMREEAETHLYKAEEEIGRKTFEGPTPFEKAMENLASLRAPVDAFFDHVTVNASEPELRVNRL
QLLNELRQAVHRIADFSKIAG
>Mature_820_residues
PDLLLELFSEEIPARMQAQAAEDLKRLVTGALSEQGLSFEGAASFATPRRLALHVAGLPIRQADVREEKKGPKVGAPEAA
LQGFLKSAGLASLDQAIVQKDAKKGDFYVAVIERAGRPALDVLADILPAVIKSFPWPKSMRWGRASARSDALRWVRPLHA
ILATFGPETEEPDVVPFSVEGIEAGQTTFGHRFLAPEPIKIRRFDDYLPALEKAKVVLDAERRRDIILHDARDLSFAQGL
DLIEDKALLEEVAGLVEWPVVLLAEFDPQFLEIPPEVIRATIRANQKCFVLRDPVSGRLAHKFLLVANIEASDGGKAIVA
GNARVVRARLSDAKFFWETDLQTRLEARLPKLETIVFHEKLGTQGERVQRLVLNARDLSPIVGADQAEAARAAELAKTDL
VSEMVGEFPELQGLMGRYYAQKQGESSNVAAAIEEHYKPQGPNDLVPNAPVSIAVALADKLDSLVGFFAIDERPSGSKDP
YALRRAALGVIALILENKLRLPLRRQLENVFVHLVRVSEAVPAFNLLTLRDDRDADAGELARLAQGGLWAWEFAREPAKS
AHAVASRYLEARFAGEGEEREEDMASFAALHDIVKPYERLREIWLASGQAGAEDPDFIDMRQAFLAAREDLIGQLIAFFG
DRLKVYLRERGARYDLIDAVFALPGQDDLLLIVARVEALASFLDTEEGKNLLAGYRRAANILKAEEKKDGVGAFNAPADP
GLLREGQERALWERIRLMREEAETHLYKAEEEIGRKTFEGPTPFEKAMENLASLRAPVDAFFDHVTVNASEPELRVNRLQ
LLNELRQAVHRIADFSKIAG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=517, Percent_Identity=38.2978723404255, Blast_Score=328, Evalue=1e-90,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 90907; Mature: 90776

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDLLLELFSEEIPARMQAQAAEDLKRLVTGALSEQGLSFEGAASFATPRRLALHVAGLP
CHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEECCCC
IRQADVREEKKGPKVGAPEAALQGFLKSAGLASLDQAIVQKDAKKGDFYVAVIERAGRPA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEHHCCCCH
LDVLADILPAVIKSFPWPKSMRWGRASARSDALRWVRPLHAILATFGPETEEPDVVPFSV
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
EGIEAGQTTFGHRFLAPEPIKIRRFDDYLPALEKAKVVLDAERRRDIILHDARDLSFAQG
CCCCCCCCHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHEEHHHCCCEEEECCCCCHHHHC
LDLIEDKALLEEVAGLVEWPVVLLAEFDPQFLEIPPEVIRATIRANQKCFVLRDPVSGRL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCEEEEEECCCCCHH
AHKFLLVANIEASDGGKAIVAGNARVVRARLSDAKFFWETDLQTRLEARLPKLETIVFHE
HHEEEEEEEEECCCCCCEEEECCCEEEEEHHCCCHHEECCCHHHHHHHHCCHHHHHHHHH
KLGTQGERVQRLVLNARDLSPIVGADQAEAARAAELAKTDLVSEMVGEFPELQGLMGRYY
HHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AQKQGESSNVAAAIEEHYKPQGPNDLVPNAPVSIAVALADKLDSLVGFFAIDERPSGSKD
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
PYALRRAALGVIALILENKLRLPLRRQLENVFVHLVRVSEAVPAFNLLTLRDDRDADAGE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHH
LARLAQGGLWAWEFAREPAKSAHAVASRYLEARFAGEGEEREEDMASFAALHDIVKPYER
HHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LREIWLASGQAGAEDPDFIDMRQAFLAAREDLIGQLIAFFGDRLKVYLRERGARYDLIDA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VFALPGQDDLLLIVARVEALASFLDTEEGKNLLAGYRRAANILKAEEKKDGVGAFNAPAD
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PGLLREGQERALWERIRLMREEAETHLYKAEEEIGRKTFEGPTPFEKAMENLASLRAPVD
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHH
AFFDHVTVNASEPELRVNRLQLLNELRQAVHRIADFSKIAG
HHHHCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PDLLLELFSEEIPARMQAQAAEDLKRLVTGALSEQGLSFEGAASFATPRRLALHVAGLP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHEEECCCC
IRQADVREEKKGPKVGAPEAALQGFLKSAGLASLDQAIVQKDAKKGDFYVAVIERAGRPA
CCHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEEEHHCCCCH
LDVLADILPAVIKSFPWPKSMRWGRASARSDALRWVRPLHAILATFGPETEEPDVVPFSV
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEE
EGIEAGQTTFGHRFLAPEPIKIRRFDDYLPALEKAKVVLDAERRRDIILHDARDLSFAQG
CCCCCCCCHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHEEHHHCCCEEEECCCCCHHHHC
LDLIEDKALLEEVAGLVEWPVVLLAEFDPQFLEIPPEVIRATIRANQKCFVLRDPVSGRL
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHCCCEEEEEECCCCCHH
AHKFLLVANIEASDGGKAIVAGNARVVRARLSDAKFFWETDLQTRLEARLPKLETIVFHE
HHEEEEEEEEECCCCCCEEEECCCEEEEEHHCCCHHEECCCHHHHHHHHCCHHHHHHHHH
KLGTQGERVQRLVLNARDLSPIVGADQAEAARAAELAKTDLVSEMVGEFPELQGLMGRYY
HHCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHH
AQKQGESSNVAAAIEEHYKPQGPNDLVPNAPVSIAVALADKLDSLVGFFAIDERPSGSKD
HHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCCCCC
PYALRRAALGVIALILENKLRLPLRRQLENVFVHLVRVSEAVPAFNLLTLRDDRDADAGE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHH
LARLAQGGLWAWEFAREPAKSAHAVASRYLEARFAGEGEEREEDMASFAALHDIVKPYER
HHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
LREIWLASGQAGAEDPDFIDMRQAFLAAREDLIGQLIAFFGDRLKVYLRERGARYDLIDA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VFALPGQDDLLLIVARVEALASFLDTEEGKNLLAGYRRAANILKAEEKKDGVGAFNAPAD
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PGLLREGQERALWERIRLMREEAETHLYKAEEEIGRKTFEGPTPFEKAMENLASLRAPVD
CCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHH
AFFDHVTVNASEPELRVNRLQLLNELRQAVHRIADFSKIAG
HHHHCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA