Click here to switch to the map view.

The map label for this gene is ampR [H]

Identifier: 182679030

GI number: 182679030

Start: 2361029

End: 2361895

Strand: Direct

Name: ampR [H]

Synonym: Bind_2066

Alternate gene names: 182679030

Gene position: 2361029-2361895 (Clockwise)

Preceding gene: 182679028

Following gene: 182679032

Centisome position: 56.62

GC content: 57.32

Gene sequence:

>867_bases
ATGTGGTGGTCTGTCTTGGAATTACCGTCGATTAAAGGACTGCAAGCGCTCGCTGCATTGAAGAAGGCAGACAGCCTTTC
CCATGCTGCCGTCTTGCTCGGCGCTACCCGATCCGCACTGTCACATCGCATCGCCGAACTGGAACGGCAATTGGGTGTCA
CCTTGATCCGTCAAGCGGGCAGACGAGCGGTTTTAACTGACGACGCGCAAGCACTTCTCCTCGTCATGGGAGACGCTCTC
GACAGGATCGAAGCCGCAATAGAGCCGCTACGGCGACGACGTCAGCAGTTGCGTATCAGCACCGTAGCGACCTTCGCATC
GCATTGGTTGCTTCCCCGTCTGCCCGACTGGCAGACACGACACCCGCACATTGATCTGTTGATCTCGACCTCAACCCGCC
CGGTCGATCTGACAGCCGAGGATTTCGACTGCGCGTTGCGCCATGGTTTGGGGAAATGGGAAGGACTTGCATCCACCCTC
CTATTTCGGGAAACGCTCGTGCCCGTGGGAAGGCCTGATGTATCTGACATTTCAAAGGCGAGTATGCTGATCCGTGCACG
CTCCCGATTTCGGGACTGGTTGCGCTGGTGGCGAGCCTGCGGGCTGCCCGGCAATCCACCCACACGTGGCGTCATCGTCG
AAACACGGGCTCAAGCGATGGATGCTGTCCTGGCAGGTGCCGGTATCACCATGATGGATGAGGCTTATATCAAGCCATAT
GTCGCAGCAGGGCGACTCCGCACGCTCGGCGAAACAATCTTTCTCGCGGAAGGCTATTACCTCGTTGTTCCAGATGCACG
ACGACAACCTTCTGAAGCCGTCAAGCTATTTAAGGCTTGGCTTTTAGACCAAGCTGCTGCTCACTAG

Upstream 100 bases:

>100_bases
AAAGGGCTCTGCTCATACCCTATCTCCTCGTTTTTCGATACGCCTCCAATATAGGGGGTTTCAGACTGCCAAAGCGTGAG
ATATTTTCACTCTTCCGATC

Downstream 100 bases:

>100_bases
GATAAGGGGCAAGCCAAAGCGTGTCTTGCTTTATGGCTCCCCCTCTACCCTTCGCCTCACTCGGGCGTATCCCCATTATC
ACTTGGCCCTGAATCTCCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MWWSVLELPSIKGLQALAALKKADSLSHAAVLLGATRSALSHRIAELERQLGVTLIRQAGRRAVLTDDAQALLLVMGDAL
DRIEAAIEPLRRRRQQLRISTVATFASHWLLPRLPDWQTRHPHIDLLISTSTRPVDLTAEDFDCALRHGLGKWEGLASTL
LFRETLVPVGRPDVSDISKASMLIRARSRFRDWLRWWRACGLPGNPPTRGVIVETRAQAMDAVLAGAGITMMDEAYIKPY
VAAGRLRTLGETIFLAEGYYLVVPDARRQPSEAVKLFKAWLLDQAAAH

Sequences:

>Translated_288_residues
MWWSVLELPSIKGLQALAALKKADSLSHAAVLLGATRSALSHRIAELERQLGVTLIRQAGRRAVLTDDAQALLLVMGDAL
DRIEAAIEPLRRRRQQLRISTVATFASHWLLPRLPDWQTRHPHIDLLISTSTRPVDLTAEDFDCALRHGLGKWEGLASTL
LFRETLVPVGRPDVSDISKASMLIRARSRFRDWLRWWRACGLPGNPPTRGVIVETRAQAMDAVLAGAGITMMDEAYIKPY
VAAGRLRTLGETIFLAEGYYLVVPDARRQPSEAVKLFKAWLLDQAAAH
>Mature_288_residues
MWWSVLELPSIKGLQALAALKKADSLSHAAVLLGATRSALSHRIAELERQLGVTLIRQAGRRAVLTDDAQALLLVMGDAL
DRIEAAIEPLRRRRQQLRISTVATFASHWLLPRLPDWQTRHPHIDLLISTSTRPVDLTAEDFDCALRHGLGKWEGLASTL
LFRETLVPVGRPDVSDISKASMLIRARSRFRDWLRWWRACGLPGNPPTRGVIVETRAQAMDAVLAGAGITMMDEAYIKPY
VAAGRLRTLGETIFLAEGYYLVVPDARRQPSEAVKLFKAWLLDQAAAH

Specific function: This protein is a positive regulator of gene expression of beta-lactamase (AmpC) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=30.7167235494881, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1786448, Length=284, Percent_Identity=27.4647887323944, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=26.0726072607261, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=22.8668941979522, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787589, Length=143, Percent_Identity=31.4685314685315, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32060; Mature: 32060

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWWSVLELPSIKGLQALAALKKADSLSHAAVLLGATRSALSHRIAELERQLGVTLIRQAG
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RRAVLTDDAQALLLVMGDALDRIEAAIEPLRRRRQQLRISTVATFASHWLLPRLPDWQTR
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HPHIDLLISTSTRPVDLTAEDFDCALRHGLGKWEGLASTLLFRETLVPVGRPDVSDISKA
CCCEEEEEECCCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SMLIRARSRFRDWLRWWRACGLPGNPPTRGVIVETRAQAMDAVLAGAGITMMDEAYIKPY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHH
VAAGRLRTLGETIFLAEGYYLVVPDARRQPSEAVKLFKAWLLDQAAAH
HHHHHHHHHCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MWWSVLELPSIKGLQALAALKKADSLSHAAVLLGATRSALSHRIAELERQLGVTLIRQAG
CCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RRAVLTDDAQALLLVMGDALDRIEAAIEPLRRRRQQLRISTVATFASHWLLPRLPDWQTR
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
HPHIDLLISTSTRPVDLTAEDFDCALRHGLGKWEGLASTLLFRETLVPVGRPDVSDISKA
CCCEEEEEECCCCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
SMLIRARSRFRDWLRWWRACGLPGNPPTRGVIVETRAQAMDAVLAGAGITMMDEAYIKPY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHH
VAAGRLRTLGETIFLAEGYYLVVPDARRQPSEAVKLFKAWLLDQAAAH
HHHHHHHHHCCEEEEECCEEEECCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8405939; 10984043; 2125210 [H]