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The map label for this gene is dme [H]

Identifier: 182679027

GI number: 182679027

Start: 2356460

End: 2358811

Strand: Reverse

Name: dme [H]

Synonym: Bind_2063

Alternate gene names: 182679027

Gene position: 2358811-2356460 (Counterclockwise)

Preceding gene: 182679029

Following gene: 182679026

Centisome position: 56.56

GC content: 60.63

Gene sequence:

>2352_bases
ATGCGGTCCAAAAGGGAGAAGAGAGATATGGTCGATTCAATACCCAACAATCTCGCCGCCAATGCTCTCCTCTATCATCG
ACTGCCACGGCCCGGGAAACTTGAAATCCAGGCGACGAAGCCACTCGCCACGCAGCGCGATCTGGCGCTTGCCTATTCGC
CGGGCGTTGCCGCCGCCTCTTTGGAAATCGCCGATAATCCCGCCGCCGCGACCGAATTGACCATTCGCCAGAATCTCGTC
GGCGTCATCTCGAATGGCACGGCGGTTTTGGGCCTTGGCAATATCGGCCCCTTGGCCTCTAAGCCTGTCATGGAAGGCAA
GGCGGTTCTGTTCAAGAAATTCGCCGGCATTGACGTGTTCGATATCGAGGTCGCGGAGACCGATGTCGATAAGCTCGTCG
AGGTGATCGCCGCGCTGGAGCCGACCTTCGGCGGCATCAATCTTGAGGATATCAAGGCGCCCGAATGTTTCGAGGTGGAG
CGCCGGCTGCGCGAGCGCATGAATATTCCGGTGTTCCATGATGATCAGCATGGCACGGCCATCATCGTCGCCGCGGCGAT
CCTCAATGGCATGGAAATGGGCGGGAAATCCCTCGCCAATGCCAAGATCGTGACCTCTGGGGCGGGCGCGGCGGCGCTCG
CCTGTCTTGATCTTCTGGTCGATCTCGGCGCCAAGCGGGACAATATTTTCGTCACCGATATTGCCGGCGTGGTCTACAAG
GGCCGTCAGGAATTGATGGACCCGCGCAAGGAAGTCTATGCGCGCGAGACCAATGCCCGCACGCTGAGTGATGTCATCGG
CGGCGCCGATGTCTTCCTCGGCCTTTCGGCGGGCGGCGTCCTGAAGCCCGAAATGCTGAAGGAAATGGAAGCCAAGCCCT
TGATCATGGCGCTGGCCAATCCCTATCCCGAAATCGACCCGGAAGCTGCCTATGCAGCGCGTTCCGATGCGATGATCTGC
ACCGGCCGGTCGGATTATCCGAACCAGGTCAATAATGTTCTCTGCTTCCCCTTCATCTTCCGGGGGGCGTTGGATGTCGC
CGCGACGACCATCAACGAGGAAATGAAGATCGCGGCGGTCAATGCCATCGCCCAATTGGCGCATGAGCCGCCTTCCGATG
TCGTCGCCCGCGCTTATGGCGGCGAGGCCCGGCAATATGGCAAGGACAGCCTCATTCCTTCCCCCTTCGATCCCCGGCTG
ATCCTGCGCATCGCGCCGGCCGTCGCCAAGGCGGCCATGGCGACGGGCGTTGCGCGCAAGCCCATCGAGGATTTCGTGGC
CTATGAGGAAGTGCTGTCGCGCTTCGTCTTCCGCTCGGGCTTCATCATGAAGCCGGTGATCCAGGCCGCCAAAGCCAATC
CCAAACGGGTCATTTATGCCGAGGGCGAGGATGAACGCGTCCTGCGCGCCGTGCAATCGGTGGTCGAGGAGGGCATTGCC
TATCCGATCCTCATCGGTCGTCCCGATGTCATCAATTCCCGTGTCGAGCGCTTTGGCCTCTCGATCCGCCAGGATCGCGA
TTTCGAGCTCATCAACCCGAATGATGATCCGCGCTATAAGGATTATGTCGCGACCTATCTCGAGGCCGCCGGCCGTCACG
GCATTACACCCGATGCCGCGCGCACGCTCGTGCGCACCAATTCGACGGTGATTGCCGCGCTTGCCGTCAAACGCGGCGAT
GCCGATGCCATGCTGTGCGGCTTGGAAGGCCGTTTCCATAGCCGCCTGCGGCATATCAAGGACATTATCGGTCTGGTGCC
GAATGCCACGGATTTTTCGGCGATGAGCTTGATGGTGACGAATCACGGCGCCTTCTTCCTCACCGATACGCATGTGCGGC
GCGATCCGGCCGCTTTCGAGATCGCGGAAATGGCATTGATGTGCGCGAGCCACGTCAGGCGTTTCGGCCTGACCCCCAAG
ATCGCGCTCGTCTCGCATTCGGATTTCGGCTCGGATGACGCGCCTTCCGCCTTGAAGATGCGCAATGCGCTGGAAATTCT
CCACCGTCGCGCGCCTGATCTCGAGGTCGATGGCGAAATGCAGGCTGATACGGCTCTGTCCCAGGCGATCCGCGATCGCG
TCATGCCGAATTCGCGCCTTAGGGGCGAGGCCAATGTCCTGGTCATGCCCAACCTCGATTCAGCCAATATTGCTTTTGCC
ATGACCAAAATGATGGCGGATGCGCTGCCGGTCGGCCCGATCCTGATCGGCGCGGCCAAACCGGCCCATGTGCTCACGCC
TTCGGTCACCTCGCGCGGTATCGTCAATATGACGGCGGTCTCCGTGGCCGAGGCGCAAAGTGATACGGCGATCGAGAGAG
AGGAAGTGCTTTCGCGCGTCGGTCTCGTCTGA

Upstream 100 bases:

>100_bases
AGAAGCCTTGCGGGCGCAAAATCCTGCCGTAAAGCCTATGGTAAACAGCTTTTCCTCCCTCATATTGGGGGGTAGAGTAG
AGAAAGGTGGACCGAACTCT

Downstream 100 bases:

>100_bases
GGTCTGAGAAAACACGAAACCCAATCTAGGAAAAGCGGTGGCGGCGCCAAAAGCGTTGCCCCGCTTTTTTCTTTTTCTTT
TTTCTCGCCAGCGCTCGGTG

Product: malic enzyme

Products: NA

Alternate protein names: NAD-ME [H]

Number of amino acids: Translated: 783; Mature: 783

Protein sequence:

>783_residues
MRSKREKRDMVDSIPNNLAANALLYHRLPRPGKLEIQATKPLATQRDLALAYSPGVAAASLEIADNPAAATELTIRQNLV
GVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEVAETDVDKLVEVIAALEPTFGGINLEDIKAPECFEVE
RRLRERMNIPVFHDDQHGTAIIVAAAILNGMEMGGKSLANAKIVTSGAGAAALACLDLLVDLGAKRDNIFVTDIAGVVYK
GRQELMDPRKEVYARETNARTLSDVIGGADVFLGLSAGGVLKPEMLKEMEAKPLIMALANPYPEIDPEAAYAARSDAMIC
TGRSDYPNQVNNVLCFPFIFRGALDVAATTINEEMKIAAVNAIAQLAHEPPSDVVARAYGGEARQYGKDSLIPSPFDPRL
ILRIAPAVAKAAMATGVARKPIEDFVAYEEVLSRFVFRSGFIMKPVIQAAKANPKRVIYAEGEDERVLRAVQSVVEEGIA
YPILIGRPDVINSRVERFGLSIRQDRDFELINPNDDPRYKDYVATYLEAAGRHGITPDAARTLVRTNSTVIAALAVKRGD
ADAMLCGLEGRFHSRLRHIKDIIGLVPNATDFSAMSLMVTNHGAFFLTDTHVRRDPAAFEIAEMALMCASHVRRFGLTPK
IALVSHSDFGSDDAPSALKMRNALEILHRRAPDLEVDGEMQADTALSQAIRDRVMPNSRLRGEANVLVMPNLDSANIAFA
MTKMMADALPVGPILIGAAKPAHVLTPSVTSRGIVNMTAVSVAEAQSDTAIEREEVLSRVGLV

Sequences:

>Translated_783_residues
MRSKREKRDMVDSIPNNLAANALLYHRLPRPGKLEIQATKPLATQRDLALAYSPGVAAASLEIADNPAAATELTIRQNLV
GVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEVAETDVDKLVEVIAALEPTFGGINLEDIKAPECFEVE
RRLRERMNIPVFHDDQHGTAIIVAAAILNGMEMGGKSLANAKIVTSGAGAAALACLDLLVDLGAKRDNIFVTDIAGVVYK
GRQELMDPRKEVYARETNARTLSDVIGGADVFLGLSAGGVLKPEMLKEMEAKPLIMALANPYPEIDPEAAYAARSDAMIC
TGRSDYPNQVNNVLCFPFIFRGALDVAATTINEEMKIAAVNAIAQLAHEPPSDVVARAYGGEARQYGKDSLIPSPFDPRL
ILRIAPAVAKAAMATGVARKPIEDFVAYEEVLSRFVFRSGFIMKPVIQAAKANPKRVIYAEGEDERVLRAVQSVVEEGIA
YPILIGRPDVINSRVERFGLSIRQDRDFELINPNDDPRYKDYVATYLEAAGRHGITPDAARTLVRTNSTVIAALAVKRGD
ADAMLCGLEGRFHSRLRHIKDIIGLVPNATDFSAMSLMVTNHGAFFLTDTHVRRDPAAFEIAEMALMCASHVRRFGLTPK
IALVSHSDFGSDDAPSALKMRNALEILHRRAPDLEVDGEMQADTALSQAIRDRVMPNSRLRGEANVLVMPNLDSANIAFA
MTKMMADALPVGPILIGAAKPAHVLTPSVTSRGIVNMTAVSVAEAQSDTAIEREEVLSRVGLV
>Mature_783_residues
MRSKREKRDMVDSIPNNLAANALLYHRLPRPGKLEIQATKPLATQRDLALAYSPGVAAASLEIADNPAAATELTIRQNLV
GVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVFDIEVAETDVDKLVEVIAALEPTFGGINLEDIKAPECFEVE
RRLRERMNIPVFHDDQHGTAIIVAAAILNGMEMGGKSLANAKIVTSGAGAAALACLDLLVDLGAKRDNIFVTDIAGVVYK
GRQELMDPRKEVYARETNARTLSDVIGGADVFLGLSAGGVLKPEMLKEMEAKPLIMALANPYPEIDPEAAYAARSDAMIC
TGRSDYPNQVNNVLCFPFIFRGALDVAATTINEEMKIAAVNAIAQLAHEPPSDVVARAYGGEARQYGKDSLIPSPFDPRL
ILRIAPAVAKAAMATGVARKPIEDFVAYEEVLSRFVFRSGFIMKPVIQAAKANPKRVIYAEGEDERVLRAVQSVVEEGIA
YPILIGRPDVINSRVERFGLSIRQDRDFELINPNDDPRYKDYVATYLEAAGRHGITPDAARTLVRTNSTVIAALAVKRGD
ADAMLCGLEGRFHSRLRHIKDIIGLVPNATDFSAMSLMVTNHGAFFLTDTHVRRDPAAFEIAEMALMCASHVRRFGLTPK
IALVSHSDFGSDDAPSALKMRNALEILHRRAPDLEVDGEMQADTALSQAIRDRVMPNSRLRGEANVLVMPNLDSANIAFA
MTKMMADALPVGPILIGAAKPAHVLTPSVTSRGIVNMTAVSVAEAQSDTAIEREEVLSRVGLV

Specific function: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria [H]

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4505143, Length=342, Percent_Identity=23.3918128654971, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI62420882, Length=348, Percent_Identity=24.4252873563218, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI62420880, Length=348, Percent_Identity=24.4252873563218, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI239049447, Length=348, Percent_Identity=24.4252873563218, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1788806, Length=754, Percent_Identity=57.1618037135278, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI1788800, Length=329, Percent_Identity=31.306990881459, Blast_Score=145, Evalue=1e-35,
Organism=Escherichia coli, GI1788635, Length=324, Percent_Identity=26.5432098765432, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI87081919, Length=294, Percent_Identity=26.8707482993197, Blast_Score=73, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17537199, Length=357, Percent_Identity=24.0896358543417, Blast_Score=68, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322823, Length=271, Percent_Identity=26.5682656826568, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI281362674, Length=457, Percent_Identity=24.2888402625821, Blast_Score=83, Evalue=9e-16,
Organism=Drosophila melanogaster, GI281362672, Length=422, Percent_Identity=24.8815165876777, Blast_Score=82, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356279, Length=422, Percent_Identity=24.8815165876777, Blast_Score=82, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.39 [H]

Molecular weight: Translated: 84503; Mature: 84503

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSKREKRDMVDSIPNNLAANALLYHRLPRPGKLEIQATKPLATQRDLALAYSPGVAAAS
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCCCEEE
LEIADNPAAATELTIRQNLVGVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCCCHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEVAETDVDKLVEVIAALEPTFGGINLEDIKAPECFEVERRLRERMNIPVFHDDQHGTA
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCH
IIVAAAILNGMEMGGKSLANAKIVTSGAGAAALACLDLLVDLGAKRDNIFVTDIAGVVYK
HHHHHHHHHHHHCCCCHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHH
GRQELMDPRKEVYARETNARTLSDVIGGADVFLGLSAGGVLKPEMLKEMEAKPLIMALAN
CHHHHHCHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEECC
PYPEIDPEAAYAARSDAMICTGRSDYPNQVNNVLCFPFIFRGALDVAATTINEEMKIAAV
CCCCCCCCHHHHCCCCCEEEECCCCCCHHHCCEEEHHHHHHHHHHHHHHHHCCCHHHHHH
NAIAQLAHEPPSDVVARAYGGEARQYGKDSLIPSPFDPRLILRIAPAVAKAAMATGVARK
HHHHHHHCCCCHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
PIEDFVAYEEVLSRFVFRSGFIMKPVIQAAKANPKRVIYAEGEDERVLRAVQSVVEEGIA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
YPILIGRPDVINSRVERFGLSIRQDRDFELINPNDDPRYKDYVATYLEAAGRHGITPDAA
EEEEECCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHH
RTLVRTNSTVIAALAVKRGDADAMLCGLEGRFHSRLRHIKDIIGLVPNATDFSAMSLMVT
HHHHHCCCEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEE
NHGAFFLTDTHVRRDPAAFEIAEMALMCASHVRRFGLTPKIALVSHSDFGSDDAPSALKM
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHH
RNALEILHRRAPDLEVDGEMQADTALSQAIRDRVMPNSRLRGEANVLVMPNLDSANIAFA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHH
MTKMMADALPVGPILIGAAKPAHVLTPSVTSRGIVNMTAVSVAEAQSDTAIEREEVLSRV
HHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHH
GLV
CCC
>Mature Secondary Structure
MRSKREKRDMVDSIPNNLAANALLYHRLPRPGKLEIQATKPLATQRDLALAYSPGVAAAS
CCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEECCCCCEEE
LEIADNPAAATELTIRQNLVGVISNGTAVLGLGNIGPLASKPVMEGKAVLFKKFAGIDVF
EEECCCCCHHHHHHHHHHHHEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCCEEE
DIEVAETDVDKLVEVIAALEPTFGGINLEDIKAPECFEVERRLRERMNIPVFHDDQHGTA
EEEECCCCHHHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEECCCCCCH
IIVAAAILNGMEMGGKSLANAKIVTSGAGAAALACLDLLVDLGAKRDNIFVTDIAGVVYK
HHHHHHHHHHHHCCCCHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHH
GRQELMDPRKEVYARETNARTLSDVIGGADVFLGLSAGGVLKPEMLKEMEAKPLIMALAN
CHHHHHCHHHHHHHHCCCCHHHHHHHCCCCEEEEECCCCCCCHHHHHHCCCCCEEEEECC
PYPEIDPEAAYAARSDAMICTGRSDYPNQVNNVLCFPFIFRGALDVAATTINEEMKIAAV
CCCCCCCCHHHHCCCCCEEEECCCCCCHHHCCEEEHHHHHHHHHHHHHHHHCCCHHHHHH
NAIAQLAHEPPSDVVARAYGGEARQYGKDSLIPSPFDPRLILRIAPAVAKAAMATGVARK
HHHHHHHCCCCHHHHHHHCCCHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHC
PIEDFVAYEEVLSRFVFRSGFIMKPVIQAAKANPKRVIYAEGEDERVLRAVQSVVEEGIA
CHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
YPILIGRPDVINSRVERFGLSIRQDRDFELINPNDDPRYKDYVATYLEAAGRHGITPDAA
EEEEECCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCHHHH
RTLVRTNSTVIAALAVKRGDADAMLCGLEGRFHSRLRHIKDIIGLVPNATDFSAMSLMVT
HHHHHCCCEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCHHEEEEEE
NHGAFFLTDTHVRRDPAAFEIAEMALMCASHVRRFGLTPKIALVSHSDFGSDDAPSALKM
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHH
RNALEILHRRAPDLEVDGEMQADTALSQAIRDRVMPNSRLRGEANVLVMPNLDSANIAFA
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHH
MTKMMADALPVGPILIGAAKPAHVLTPSVTSRGIVNMTAVSVAEAQSDTAIEREEVLSRV
HHHHHHHHCCCCCEEEECCCCCEEECCCCCCCCCEEEHHHHHHHCCCCCHHHHHHHHHHH
GLV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9535928; 11481430; 10407149 [H]