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The map label for this gene is otsB [H]

Identifier: 182678942

GI number: 182678942

Start: 2259908

End: 2260693

Strand: Reverse

Name: otsB [H]

Synonym: Bind_1976

Alternate gene names: 182678942

Gene position: 2260693-2259908 (Counterclockwise)

Preceding gene: 182678944

Following gene: 182678941

Centisome position: 54.21

GC content: 54.71

Gene sequence:

>786_bases
ATGCTCGAACCGACCGCACGAATCAAGGACACGACAATCAAGGACACGAAGCCTGCGGCAGAGCCCGGGCTGTGGAAGCT
GATGCCTTGCAGCACCAGCGCCTTTTTCTTCGATGTCGATGGGACTTTGCTCGATATCAAGCCCCATCCGGCCGATGTTG
TCGCGGATGCGGGTTTGAAAAGCCTTTTGCTCCAATTGGCCGCCAAAACCAAAGGGGCTTTGGCCCTGGTGAGCGGGCGC
ATGATCGAGGATATAGACCGGATCTTCGTGCCACTCGTGCTCCCAGCCGCAGGTCTTCACGGCGGGCAAATTCGTCTCTC
CCAGACGGTTGAAATGCTGGCCTCGCGGCAAAATATTGATCTGGCAAGGCCAGAGGTGTCGGCCTTTGTCGATAAATTTC
AAGGTCTCATGCTGGAAGACAAGGGGGCCACGCTTGCGATTCATTTTCGCCAATGCCCGGAACTTTCCGAAATCGTGCGC
ATTAGTGTCGAGGAATTAGCGTCAAGACACGGTTTGGAAGCACAGGAAGGTAAAATGGTGGTGGAGTTGAAGCCCTCTGC
GTTCGACAAGGGTACCGCCATCACCAACTTTCTCACCGAGCCGCCTTTTTTCGGCCGGCGTCCGGTTTTCTTCGGGGATG
ATCTGACCGATGAAAAAGGGTTCGAGGCCGTTAATCGGGTTGGAGGGATTTCGGTTAAAATCGGGTCCTTCGAAACACCG
ACCAAAGCTCTCTATCGCCTTGCAGAGCCCTCCGCTCTTCGATGGCAAATCGAGCAGATGATTTAA

Upstream 100 bases:

>100_bases
GTTTCGCTATCGGATATGGTTTGAGTCATTCCGCCTCAAAATCAGCATGATCCAGCCCCATTATAAAAGTTGGCCCCGGC
AGTCCCTTTGGACCTTGTTG

Downstream 100 bases:

>100_bases
TGTGGACTTTGTCCCTTGAATCGCATGTTGCTTCAATTATACTGGGAATATTCGCAAATACTTCGTATCCAGTGCGTTAA
TTGATGATATCTTTGCCAAT

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose 6-phosphate phosphatase; Trehalose-phosphatase [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MLEPTARIKDTTIKDTKPAAEPGLWKLMPCSTSAFFFDVDGTLLDIKPHPADVVADAGLKSLLLQLAAKTKGALALVSGR
MIEDIDRIFVPLVLPAAGLHGGQIRLSQTVEMLASRQNIDLARPEVSAFVDKFQGLMLEDKGATLAIHFRQCPELSEIVR
ISVEELASRHGLEAQEGKMVVELKPSAFDKGTAITNFLTEPPFFGRRPVFFGDDLTDEKGFEAVNRVGGISVKIGSFETP
TKALYRLAEPSALRWQIEQMI

Sequences:

>Translated_261_residues
MLEPTARIKDTTIKDTKPAAEPGLWKLMPCSTSAFFFDVDGTLLDIKPHPADVVADAGLKSLLLQLAAKTKGALALVSGR
MIEDIDRIFVPLVLPAAGLHGGQIRLSQTVEMLASRQNIDLARPEVSAFVDKFQGLMLEDKGATLAIHFRQCPELSEIVR
ISVEELASRHGLEAQEGKMVVELKPSAFDKGTAITNFLTEPPFFGRRPVFFGDDLTDEKGFEAVNRVGGISVKIGSFETP
TKALYRLAEPSALRWQIEQMI
>Mature_261_residues
MLEPTARIKDTTIKDTKPAAEPGLWKLMPCSTSAFFFDVDGTLLDIKPHPADVVADAGLKSLLLQLAAKTKGALALVSGR
MIEDIDRIFVPLVLPAAGLHGGQIRLSQTVEMLASRQNIDLARPEVSAFVDKFQGLMLEDKGATLAIHFRQCPELSEIVR
ISVEELASRHGLEAQEGKMVVELKPSAFDKGTAITNFLTEPPFFGRRPVFFGDDLTDEKGFEAVNRVGGISVKIGSFETP
TKALYRLAEPSALRWQIEQMI

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=231, Percent_Identity=40.2597402597403, Blast_Score=160, Evalue=1e-40,
Organism=Drosophila melanogaster, GI19920676, Length=207, Percent_Identity=27.536231884058, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI221473368, Length=253, Percent_Identity=28.4584980237154, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI20129307, Length=253, Percent_Identity=28.4584980237154, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582490, Length=253, Percent_Identity=28.4584980237154, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 28574; Mature: 28574

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEPTARIKDTTIKDTKPAAEPGLWKLMPCSTSAFFFDVDGTLLDIKPHPADVVADAGLK
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCEEEEECCCCHHHHHHHHHH
SLLLQLAAKTKGALALVSGRMIEDIDRIFVPLVLPAAGLHGGQIRLSQTVEMLASRQNID
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCC
LARPEVSAFVDKFQGLMLEDKGATLAIHFRQCPELSEIVRISVEELASRHGLEAQEGKMV
CCCHHHHHHHHHHCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
VELKPSAFDKGTAITNFLTEPPFFGRRPVFFGDDLTDEKGFEAVNRVGGISVKIGSFETP
EEECCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCEEEEECCCCCH
TKALYRLAEPSALRWQIEQMI
HHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MLEPTARIKDTTIKDTKPAAEPGLWKLMPCSTSAFFFDVDGTLLDIKPHPADVVADAGLK
CCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCEEEEECCCCHHHHHHHHHH
SLLLQLAAKTKGALALVSGRMIEDIDRIFVPLVLPAAGLHGGQIRLSQTVEMLASRQNID
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCC
LARPEVSAFVDKFQGLMLEDKGATLAIHFRQCPELSEIVRISVEELASRHGLEAQEGKMV
CCCHHHHHHHHHHCCEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCEEE
VELKPSAFDKGTAITNFLTEPPFFGRRPVFFGDDLTDEKGFEAVNRVGGISVKIGSFETP
EEECCCCCCCCCHHHHHHCCCCCCCCCCEEECCCCCCCHHHHHHHHCCCEEEEECCCCCH
TKALYRLAEPSALRWQIEQMI
HHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]