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The map label for this gene is cysS

Identifier: 182678882

GI number: 182678882

Start: 2184954

End: 2186330

Strand: Reverse

Name: cysS

Synonym: Bind_1915

Alternate gene names: 182678882

Gene position: 2186330-2184954 (Counterclockwise)

Preceding gene: 182678884

Following gene: 182678881

Centisome position: 52.43

GC content: 54.18

Gene sequence:

>1377_bases
ATGGCTTTGCAGCTCTATAATACGCTGACCCGTCAAAAAGACATTTTTGAGCCGATCGATCCGGCCAATGTGCGGCTCTA
TGCCTGTGGCCCGACGGTTTATGATTTCCTCCATATCGGCAATGGCCGCATGCTGATCGTCTTCGATCTGCTGTTCCGCG
TCCTGCGCGATCTTTATGGCGCCGATCACGTCCGCTACGTCCGCAACATCACCGATGTCGATGACAAGATCAATGCGCGC
GCTGCCGAACGCGGCATCGATATTCGGGTCCTGACCGATGAAATGACGGCGATCTTCCATGAGGACGCTAAGGCCCTGAA
CTGCCTGCCGCCCACGGTCGAGCCGCGCGCCACCGAGCATATGGCTGAGATGATCGCCATTATCGAAAAGCTCGTCGCCA
ATGGCCATGCCTATATCGCGGAAGGCCATGTGCTGTTCGATGTGCCCTCCATGCCGGATTACGGCAAGCTCTCCAAACGC
CCGCTCGATGATATGATCGCCGGTGCGCGCGTCGAGGTCGCGCCCTATAAGCGTTCACCGACCGATTTCGTTCTGTGGAA
ACCGGCCAAGCCTGAGGAACCCGGCTGGGAAAGTCCCTGGGGGCGGGGGCGTCCCGGCTGGCATCTCGAATGCTCGGCCA
TGAGCTGGAAACATTTGGGCGAAGTTTTCGACATTCACGGCGGCGGCATTGATCTCGTCTTTCCGCACCATGAGAATGAA
GTCGCCCAGACCTGCTCGGCCTTTGGCCATGATGTGATGGCCAATGTCTGGATGCATAATGGTCATTTGCAGGTCGAGGG
ACAGAAAATGTCGAAAAGCCTCGGCAATTTCCTGACCATTCGCGATGTGCTCAAGGACTGGCCCGGCGAGACCGTGCGTT
TCACCATGCTCAAGACGCATTATCGCCAGCCGATCGATTGGACGCTTTCGAGCCTGCGCGAAAGCCAGCGTGAACTCGAC
CGCTGGTATCCCGTCGCAAGGGCTTTTGATCAAATCAATCCTGATAATATTCCCGGTATTCCGTCGTCTTTTCGTGATGC
GCTCCAGGATGATTTGAATACGCCGGAGGCCATCACAATCCTACGGCGCCTTTATAAGGATGCGTTGGAAACGACTGATT
ATGCTGGTGTGCATCTCTTACTTTGTGGCAAATTGCTTGGCCTATTCGACCAATCTCTTGAGAAGTGGAAGAATTGGAAA
CCAGCAAGTTTCACAGTTGAAAACGTAACATTTGAGGACTTAATAAAAGAAAGAGAAGAGGCTAGGGCGGCAAAGAATTG
GCCGAAATCTGACCATCTTCGTGACGTGCTCGCGTCTTATGGAATTGTACTCAAGGATAATAAAGACGGCACGACGAGCT
GGGAGGCGAAACGATGA

Upstream 100 bases:

>100_bases
GCTAGCCCATGCCGGCTAGCCGGGTGCTGGCTGGAGCCGTCCTGTTCTCTTCATCGAGTTCGTGTCCGACGCCCCGGCCC
ATCAGGCTTTGGGGAAAGTC

Downstream 100 bases:

>100_bases
GCGCCGCGCCGGCTCCCGCTTTGCGTCCCTATTTGCCCTCGGATGCGCCGGTTTTGGCGGGCTTGTTTCAAGCCAGTATC
GAGGAACTGGCCGCCGAGGA

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MALQLYNTLTRQKDIFEPIDPANVRLYACGPTVYDFLHIGNGRMLIVFDLLFRVLRDLYGADHVRYVRNITDVDDKINAR
AAERGIDIRVLTDEMTAIFHEDAKALNCLPPTVEPRATEHMAEMIAIIEKLVANGHAYIAEGHVLFDVPSMPDYGKLSKR
PLDDMIAGARVEVAPYKRSPTDFVLWKPAKPEEPGWESPWGRGRPGWHLECSAMSWKHLGEVFDIHGGGIDLVFPHHENE
VAQTCSAFGHDVMANVWMHNGHLQVEGQKMSKSLGNFLTIRDVLKDWPGETVRFTMLKTHYRQPIDWTLSSLRESQRELD
RWYPVARAFDQINPDNIPGIPSSFRDALQDDLNTPEAITILRRLYKDALETTDYAGVHLLLCGKLLGLFDQSLEKWKNWK
PASFTVENVTFEDLIKEREEARAAKNWPKSDHLRDVLASYGIVLKDNKDGTTSWEAKR

Sequences:

>Translated_458_residues
MALQLYNTLTRQKDIFEPIDPANVRLYACGPTVYDFLHIGNGRMLIVFDLLFRVLRDLYGADHVRYVRNITDVDDKINAR
AAERGIDIRVLTDEMTAIFHEDAKALNCLPPTVEPRATEHMAEMIAIIEKLVANGHAYIAEGHVLFDVPSMPDYGKLSKR
PLDDMIAGARVEVAPYKRSPTDFVLWKPAKPEEPGWESPWGRGRPGWHLECSAMSWKHLGEVFDIHGGGIDLVFPHHENE
VAQTCSAFGHDVMANVWMHNGHLQVEGQKMSKSLGNFLTIRDVLKDWPGETVRFTMLKTHYRQPIDWTLSSLRESQRELD
RWYPVARAFDQINPDNIPGIPSSFRDALQDDLNTPEAITILRRLYKDALETTDYAGVHLLLCGKLLGLFDQSLEKWKNWK
PASFTVENVTFEDLIKEREEARAAKNWPKSDHLRDVLASYGIVLKDNKDGTTSWEAKR
>Mature_457_residues
ALQLYNTLTRQKDIFEPIDPANVRLYACGPTVYDFLHIGNGRMLIVFDLLFRVLRDLYGADHVRYVRNITDVDDKINARA
AERGIDIRVLTDEMTAIFHEDAKALNCLPPTVEPRATEHMAEMIAIIEKLVANGHAYIAEGHVLFDVPSMPDYGKLSKRP
LDDMIAGARVEVAPYKRSPTDFVLWKPAKPEEPGWESPWGRGRPGWHLECSAMSWKHLGEVFDIHGGGIDLVFPHHENEV
AQTCSAFGHDVMANVWMHNGHLQVEGQKMSKSLGNFLTIRDVLKDWPGETVRFTMLKTHYRQPIDWTLSSLRESQRELDR
WYPVARAFDQINPDNIPGIPSSFRDALQDDLNTPEAITILRRLYKDALETTDYAGVHLLLCGKLLGLFDQSLEKWKNWKP
ASFTVENVTFEDLIKEREEARAAKNWPKSDHLRDVLASYGIVLKDNKDGTTSWEAKR

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=510, Percent_Identity=33.7254901960784, Blast_Score=254, Evalue=2e-67,
Organism=Homo sapiens, GI10835051, Length=230, Percent_Identity=42.1739130434783, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI62240992, Length=230, Percent_Identity=42.1739130434783, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI21269877, Length=230, Percent_Identity=42.1739130434783, Blast_Score=204, Evalue=2e-52,
Organism=Homo sapiens, GI303304987, Length=230, Percent_Identity=42.1739130434783, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI1786737, Length=465, Percent_Identity=47.741935483871, Blast_Score=409, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17509795, Length=310, Percent_Identity=33.8709677419355, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17509797, Length=310, Percent_Identity=33.8709677419355, Blast_Score=173, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6324082, Length=313, Percent_Identity=35.7827476038339, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI19922218, Length=502, Percent_Identity=33.0677290836653, Blast_Score=241, Evalue=7e-64,
Organism=Drosophila melanogaster, GI19922364, Length=308, Percent_Identity=35.0649350649351, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_BEII9 (B2IEE1)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001833028.1
- GeneID:   6199137
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1915
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 52298; Mature: 52167

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 272-272

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQLYNTLTRQKDIFEPIDPANVRLYACGPTVYDFLHIGNGRMLIVFDLLFRVLRDLYG
CEEEHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHEECCCCEEHHHHHHHHHHHHHHC
ADHVRYVRNITDVDDKINARAAERGIDIRVLTDEMTAIFHEDAKALNCLPPTVEPRATEH
CHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MAEMIAIIEKLVANGHAYIAEGHVLFDVPSMPDYGKLSKRPLDDMIAGARVEVAPYKRSP
HHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCC
TDFVLWKPAKPEEPGWESPWGRGRPGWHLECSAMSWKHLGEVFDIHGGGIDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCHHEEECCCCEEEECCCCCCH
VAQTCSAFGHDVMANVWMHNGHLQVEGQKMSKSLGNFLTIRDVLKDWPGETVRFTMLKTH
HHHHHHHHCHHHHHHHEEECCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
YRQPIDWTLSSLRESQRELDRWYPVARAFDQINPDNIPGIPSSFRDALQDDLNTPEAITI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LRRLYKDALETTDYAGVHLLLCGKLLGLFDQSLEKWKNWKPASFTVENVTFEDLIKEREE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
ARAAKNWPKSDHLRDVLASYGIVLKDNKDGTTSWEAKR
HHHHHCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
ALQLYNTLTRQKDIFEPIDPANVRLYACGPTVYDFLHIGNGRMLIVFDLLFRVLRDLYG
EEEHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHEECCCCEEHHHHHHHHHHHHHHC
ADHVRYVRNITDVDDKINARAAERGIDIRVLTDEMTAIFHEDAKALNCLPPTVEPRATEH
CHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
MAEMIAIIEKLVANGHAYIAEGHVLFDVPSMPDYGKLSKRPLDDMIAGARVEVAPYKRSP
HHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCC
TDFVLWKPAKPEEPGWESPWGRGRPGWHLECSAMSWKHLGEVFDIHGGGIDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHCHHEEECCCCEEEECCCCCCH
VAQTCSAFGHDVMANVWMHNGHLQVEGQKMSKSLGNFLTIRDVLKDWPGETVRFTMLKTH
HHHHHHHHCHHHHHHHEEECCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEEEEHHHH
YRQPIDWTLSSLRESQRELDRWYPVARAFDQINPDNIPGIPSSFRDALQDDLNTPEAITI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
LRRLYKDALETTDYAGVHLLLCGKLLGLFDQSLEKWKNWKPASFTVENVTFEDLIKEREE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
ARAAKNWPKSDHLRDVLASYGIVLKDNKDGTTSWEAKR
HHHHHCCCCHHHHHHHHHHCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA