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The map label for this gene is glcB [H]

Identifier: 182678865

GI number: 182678865

Start: 2163535

End: 2165718

Strand: Direct

Name: glcB [H]

Synonym: Bind_1898

Alternate gene names: 182678865

Gene position: 2163535-2165718 (Clockwise)

Preceding gene: 182678863

Following gene: 182678866

Centisome position: 51.88

GC content: 59.39

Gene sequence:

>2184_bases
ATGGGTTATCTTTCGATCAACGGCCTTTCGGTCGATTCGGAACTTCACGATTTTATCGTCAAGGAAGCTATTCCCGGCTC
TGGCGTCAGCGCCGAACAATTTTGGACTGGCCTTGCCGATCTCGTCGCCGCGAAAAGCGCGACGAACCGCGCCTTGCTCG
CCAAGCGCGATGAATTGCAGGCCAAGATCGATGCTTATTATCGCTCCGGCGGCTCAAAGGATCCGGCTGCGACGGAAGCC
TTCCTGCGCGAGATCGGCTATCTTCTGCCCGAGCCCGCGCCCCAGGCGATCGAGACGCAAAATGTCGACGATGAAATCGC
CACGATCGCCGGGCCACAGCTCGTCGTGCCGGGCTCCAATGCCCGCTATGCGCTGAATGCCGCCAATGCCCGTTGGGGCA
GCCTCTATGACGCGCTCTATGGCACGGATGCGATTCCGGAAACCAATGGCGCGGAGCGCGGCAAGGGTTTCAACCCCACG
CGTGGCGCGCTCGTCGTCGCCAAGGCCAAGACCATTCTCGACCAGTCCGTGCCGCTTGACGGCGCGTCCTATGCGGATGT
CACGGCCTATCAGATCGAGGGGAGCACCCTGAAGGCCACGACGAAGGATGGCAAGAGCATCGGCCTGAAGACGCCCGAGC
AATTCGCTGGTTTCACCGGCACCAAGGAAGCGCCAGCCACCATCCTTTTGCGTCATAACCATCTCCATTTCGAGCTCGTC
GTCAATGCCAACCATCCGGTCGGCAAGACGGATCCGGCCGGCCTCGCCGATGTCATCATCGAGTCCGCCGTGTCGACCAT
CATCGACATGGAAGACAGTGTTGCCGTGGTCGATGCCGCCGATAAAGTGGCGCTTTATCGCAATATTCTCGGCCTGATGC
AGGGCACGCTTTCGGACACATTCGTCAAGAATGGCGCTTCGCTGACCCGCACCCTGCATGACGATCGGATCTACCAGACT
GGCGATGGCGGCACCTTGCGCCTGCATGGGCGCAGCCTGCTGCTCATCCGCAATGTCGGCCATCATATGTTCACCGACGC
GGTCCTTGATGCGTCGGGTGCCGAAATCCCGGAAAATATGCTCGACGGCATGGTGACAGCGCTTGTCGCGCGCCATGATC
TTTATGGCACCCATGAACACCATAACAGCCGCAAGGGTTCGGTCTATATCGTCAAGCCCAAAATGCATGGGCCACAGGAA
GTGGCTTTTGCCAATTCCGTGTTCGAACATATCGAACTGGCGATCGGTCTGCCCGCGAATACGCTGAAGATCGGCATCAT
GGACGAGGAACGGCGCACGACCGTCAATCTGATGGCGGGGATCCAAGCGGCCTCCAAGCGTATTGTCTTCATCAATACGG
GCTTCCTCGATCGCACCGGCGACGAAATCCATACGGCGATGGAAGCCGGCCCCATGGTGCGCAAGAACAGCATCAAGAAC
GAAGCCTGGCTGAAAGCCTATGAAGACAATAATGTGGATGTCGGCCTGGCGAGTGGCCTGCCCGGCCATGCTCAGATCGG
CAAGGGCATGTGGGCCGCTCCCGATGCCATGGCCGATATGCTGACTCAGAAGATCGGTCATCCGCGCGCCGGCGCCAATA
CGGCCTGGGTGCCCTCGCCCACCGCCGCGACCTTGCATGCGCTTCACTATCATGAAGTGGATGTCTTCGAGCGGCAGAAG
GAACTGGCTGGACGGCCGCGTGCCAAACTGCATGATCTTCTAACCGTGCCGGTCGCCAAAACGACCTATTCTGCGCAGGA
GATTCAGGAAGAGCTCGACAATAACGCCCAGGGTCTGCTCGGCTATGTCGTGCGCTGGATCGACCAGGGCGTCGGCTGTT
CGAAAGTGCCGGACATTCACAATGTCGCCTTGATGGAAGATCGCGCGACCTTGCGGATTTCCAGCCAGCATATTGCCAAC
TGGCTATATCACAAGATCGTCACCGAGAGCCAAGTCCTGGAAACCCTGAAGCGCATGGCGGTCGTCGTCGATCAGCAGAA
TGCCGGCGATTCCGCCTACCGGCCGATGGCACCCGCCTTCGACGGCATCGCTTTCAAAGCGGCCTGCGATCTCGTCTTTA
AGGGACGTGAACAGCCAAACGGCTATACGGAATTCCTGCTGCATTCACGCCGTCGCGAGGTCAAGGCCGAACAGGCCCAA
AGTGGCCAAGCCTCTGCGGCTTAA

Upstream 100 bases:

>100_bases
AGATGCTGGAAGCCCGTGAATTTCTGCCCCTCTAATGGGGTGGGACAGTTCCGGGCAAGCCTCCCGCCTTACGGGTTTGA
GCCAAATTTCGGAGAGCCAC

Downstream 100 bases:

>100_bases
GATGGGTTTTGAGCGGGGGCTTGAGTGCGGCTTGAGCCCCGCCGAAACCTGATCTTTCTTCTGCCGTTCAAGGCGTGACG
GAGGTTCCGGCTGGCCCGGG

Product: malate synthase G

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 727; Mature: 726

Protein sequence:

>727_residues
MGYLSINGLSVDSELHDFIVKEAIPGSGVSAEQFWTGLADLVAAKSATNRALLAKRDELQAKIDAYYRSGGSKDPAATEA
FLREIGYLLPEPAPQAIETQNVDDEIATIAGPQLVVPGSNARYALNAANARWGSLYDALYGTDAIPETNGAERGKGFNPT
RGALVVAKAKTILDQSVPLDGASYADVTAYQIEGSTLKATTKDGKSIGLKTPEQFAGFTGTKEAPATILLRHNHLHFELV
VNANHPVGKTDPAGLADVIIESAVSTIIDMEDSVAVVDAADKVALYRNILGLMQGTLSDTFVKNGASLTRTLHDDRIYQT
GDGGTLRLHGRSLLLIRNVGHHMFTDAVLDASGAEIPENMLDGMVTALVARHDLYGTHEHHNSRKGSVYIVKPKMHGPQE
VAFANSVFEHIELAIGLPANTLKIGIMDEERRTTVNLMAGIQAASKRIVFINTGFLDRTGDEIHTAMEAGPMVRKNSIKN
EAWLKAYEDNNVDVGLASGLPGHAQIGKGMWAAPDAMADMLTQKIGHPRAGANTAWVPSPTAATLHALHYHEVDVFERQK
ELAGRPRAKLHDLLTVPVAKTTYSAQEIQEELDNNAQGLLGYVVRWIDQGVGCSKVPDIHNVALMEDRATLRISSQHIAN
WLYHKIVTESQVLETLKRMAVVVDQQNAGDSAYRPMAPAFDGIAFKAACDLVFKGREQPNGYTEFLLHSRRREVKAEQAQ
SGQASAA

Sequences:

>Translated_727_residues
MGYLSINGLSVDSELHDFIVKEAIPGSGVSAEQFWTGLADLVAAKSATNRALLAKRDELQAKIDAYYRSGGSKDPAATEA
FLREIGYLLPEPAPQAIETQNVDDEIATIAGPQLVVPGSNARYALNAANARWGSLYDALYGTDAIPETNGAERGKGFNPT
RGALVVAKAKTILDQSVPLDGASYADVTAYQIEGSTLKATTKDGKSIGLKTPEQFAGFTGTKEAPATILLRHNHLHFELV
VNANHPVGKTDPAGLADVIIESAVSTIIDMEDSVAVVDAADKVALYRNILGLMQGTLSDTFVKNGASLTRTLHDDRIYQT
GDGGTLRLHGRSLLLIRNVGHHMFTDAVLDASGAEIPENMLDGMVTALVARHDLYGTHEHHNSRKGSVYIVKPKMHGPQE
VAFANSVFEHIELAIGLPANTLKIGIMDEERRTTVNLMAGIQAASKRIVFINTGFLDRTGDEIHTAMEAGPMVRKNSIKN
EAWLKAYEDNNVDVGLASGLPGHAQIGKGMWAAPDAMADMLTQKIGHPRAGANTAWVPSPTAATLHALHYHEVDVFERQK
ELAGRPRAKLHDLLTVPVAKTTYSAQEIQEELDNNAQGLLGYVVRWIDQGVGCSKVPDIHNVALMEDRATLRISSQHIAN
WLYHKIVTESQVLETLKRMAVVVDQQNAGDSAYRPMAPAFDGIAFKAACDLVFKGREQPNGYTEFLLHSRRREVKAEQAQ
SGQASAA
>Mature_726_residues
GYLSINGLSVDSELHDFIVKEAIPGSGVSAEQFWTGLADLVAAKSATNRALLAKRDELQAKIDAYYRSGGSKDPAATEAF
LREIGYLLPEPAPQAIETQNVDDEIATIAGPQLVVPGSNARYALNAANARWGSLYDALYGTDAIPETNGAERGKGFNPTR
GALVVAKAKTILDQSVPLDGASYADVTAYQIEGSTLKATTKDGKSIGLKTPEQFAGFTGTKEAPATILLRHNHLHFELVV
NANHPVGKTDPAGLADVIIESAVSTIIDMEDSVAVVDAADKVALYRNILGLMQGTLSDTFVKNGASLTRTLHDDRIYQTG
DGGTLRLHGRSLLLIRNVGHHMFTDAVLDASGAEIPENMLDGMVTALVARHDLYGTHEHHNSRKGSVYIVKPKMHGPQEV
AFANSVFEHIELAIGLPANTLKIGIMDEERRTTVNLMAGIQAASKRIVFINTGFLDRTGDEIHTAMEAGPMVRKNSIKNE
AWLKAYEDNNVDVGLASGLPGHAQIGKGMWAAPDAMADMLTQKIGHPRAGANTAWVPSPTAATLHALHYHEVDVFERQKE
LAGRPRAKLHDLLTVPVAKTTYSAQEIQEELDNNAQGLLGYVVRWIDQGVGCSKVPDIHNVALMEDRATLRISSQHIANW
LYHKIVTESQVLETLKRMAVVVDQQNAGDSAYRPMAPAFDGIAFKAACDLVFKGREQPNGYTEFLLHSRRREVKAEQAQS
GQASAA

Specific function: Accounts For Almost The Entire Malate-Synthesizing Activity In Cells Metabolizing Glyoxylate. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family. GlcB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789348, Length=712, Percent_Identity=58.7078651685393, Blast_Score=845, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR001465
- InterPro:   IPR006253 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 78558; Mature: 78427

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYLSINGLSVDSELHDFIVKEAIPGSGVSAEQFWTGLADLVAAKSATNRALLAKRDELQ
CCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AKIDAYYRSGGSKDPAATEAFLREIGYLLPEPAPQAIETQNVDDEIATIAGPQLVVPGSN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCC
ARYALNAANARWGSLYDALYGTDAIPETNGAERGKGFNPTRGALVVAKAKTILDQSVPLD
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCC
GASYADVTAYQIEGSTLKATTKDGKSIGLKTPEQFAGFTGTKEAPATILLRHNHLHFELV
CCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEECCEEEEEE
VNANHPVGKTDPAGLADVIIESAVSTIIDMEDSVAVVDAADKVALYRNILGLMQGTLSDT
EECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
FVKNGASLTRTLHDDRIYQTGDGGTLRLHGRSLLLIRNVGHHMFTDAVLDASGAEIPENM
HHHCCCHHHHHHCCCCCEECCCCCEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCHHH
LDGMVTALVARHDLYGTHEHHNSRKGSVYIVKPKMHGPQEVAFANSVFEHIELAIGLPAN
HHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEECCCCC
TLKIGIMDEERRTTVNLMAGIQAASKRIVFINTGFLDRTGDEIHTAMEAGPMVRKNSIKN
EEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHCCCCC
EAWLKAYEDNNVDVGLASGLPGHAQIGKGMWAAPDAMADMLTQKIGHPRAGANTAWVPSP
HHHEEEECCCCCEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
TAATLHALHYHEVDVFERQKELAGRPRAKLHDLLTVPVAKTTYSAQEIQEELDNNAQGLL
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCHHHHHHHHCCCHHHHH
GYVVRWIDQGVGCSKVPDIHNVALMEDRATLRISSQHIANWLYHKIVTESQVLETLKRMA
HHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
VVVDQQNAGDSAYRPMAPAFDGIAFKAACDLVFKGREQPNGYTEFLLHSRRREVKAEQAQ
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SGQASAA
CCCCCCC
>Mature Secondary Structure 
GYLSINGLSVDSELHDFIVKEAIPGSGVSAEQFWTGLADLVAAKSATNRALLAKRDELQ
CEEEECCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
AKIDAYYRSGGSKDPAATEAFLREIGYLLPEPAPQAIETQNVDDEIATIAGPQLVVPGSN
HHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCEEEECCCC
ARYALNAANARWGSLYDALYGTDAIPETNGAERGKGFNPTRGALVVAKAKTILDQSVPLD
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCCC
GASYADVTAYQIEGSTLKATTKDGKSIGLKTPEQFAGFTGTKEAPATILLRHNHLHFELV
CCCCCCEEEEEECCCEEEEECCCCCCCCCCCHHHHCCCCCCCCCCEEEEEEECCEEEEEE
VNANHPVGKTDPAGLADVIIESAVSTIIDMEDSVAVVDAADKVALYRNILGLMQGTLSDT
EECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHH
FVKNGASLTRTLHDDRIYQTGDGGTLRLHGRSLLLIRNVGHHMFTDAVLDASGAEIPENM
HHHCCCHHHHHHCCCCCEECCCCCEEEECCCEEEEEECCCHHHHHHHHHCCCCCCCCHHH
LDGMVTALVARHDLYGTHEHHNSRKGSVYIVKPKMHGPQEVAFANSVFEHIELAIGLPAN
HHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHEECCCCC
TLKIGIMDEERRTTVNLMAGIQAASKRIVFINTGFLDRTGDEIHTAMEAGPMVRKNSIKN
EEEEEEECCCHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHCCCHHHCCCCC
EAWLKAYEDNNVDVGLASGLPGHAQIGKGMWAAPDAMADMLTQKIGHPRAGANTAWVPSP
HHHEEEECCCCCEEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
TAATLHALHYHEVDVFERQKELAGRPRAKLHDLLTVPVAKTTYSAQEIQEELDNNAQGLL
CHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHCCCCHHHHHHHHCCCHHHHH
GYVVRWIDQGVGCSKVPDIHNVALMEDRATLRISSQHIANWLYHKIVTESQVLETLKRMA
HHHHHHHHCCCCCCCCCCCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHH
VVVDQQNAGDSAYRPMAPAFDGIAFKAACDLVFKGREQPNGYTEFLLHSRRREVKAEQAQ
EEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SGQASAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]