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The map label for this gene is gatA

Identifier: 182678845

GI number: 182678845

Start: 2142132

End: 2143613

Strand: Reverse

Name: gatA

Synonym: Bind_1878

Alternate gene names: 182678845

Gene position: 2143613-2142132 (Counterclockwise)

Preceding gene: 182678846

Following gene: 182678844

Centisome position: 51.4

GC content: 61.47

Gene sequence:

>1482_bases
GTGACCGAATTGACTGATTTGAGCCTGGCCGATGCGCGCGACGCTTTACGGCAGAAGAAGATTTCCGCGGCCGAACTCGC
CATGGCGCATATAGAGGCCGTGGAAAAGGCGCGGGCCTTGAACGCTTTTCTTCTTGAAACCCCCGAACGTGCGCAAGCCA
TGGCGCGGGCCTCTGATGAAAAGATCGCGCGCGGCGAGGCCGGGCCCCTTGAAGGCATTCCGCTTGGCATCAAGGACCTG
TTCTGCACAGAAGGCGTGCGGACGACGGCGGCGAGCCATATTCTGGAAAATTTCGTGCCCGCCTATGAATCGAGCATTAC
CGCCAATCTCTGGCGCGACGGCGCCGTGATGCTTGGCAAGCTCAATCTCGATGAATTCGCCATGGGTTCTTCGAATGAGA
CGTCCTATTTCGGGCCCGTCGTTTCACCCTGGCGGCGGGAAGGCTCCACGGCCCCTCTGACGCCGGGTGGTTCATCGGGC
GGATCCGCGGCGGCGGTCGCCGCAAGATTGTGTCTCGGGGCGACCGCGACGGATACTGGCGGGTCAATCCGCCAGCCGGC
GGCCTTTACGGGCACGGTCGGCATCAAGCCGACCTATGGCCGCTGCTCGCGCTGGGGCGTCGTGGCTTTCGCCTCCTCGC
TCGATCAAGCGGGACCGATTACCCGTACCGTGCGCGATGCGGCGATCTTGCTCCGCTCCATGGCTGGCTATGATCCAAAG
GATACGACTTCGGTCAATCTGCCAGTCCCTGATTATGAGGCGGCGCTTGGCCGTTCGATCAAGGGCATGCGGATCGGCAT
TCCGAAGGAATATCGGCTTGATGCCATGCCGGCCGAGATCGGCAAATTATGGGATCAGGGTATCGAATGGATGCGGGAAG
CGGGCGCCGAGCCAGTCGAAATCTCGCTGCCGCATACGCGCCATGCCTTGCCAGCCTATTATATCGTCGCGCCGGCTGAA
GCCTCGTCCAATCTGGCCCGGTACGATGGTGTGCGTTTCGGCCTGCGGGTGCCGGGCAAGGACATTGCCGGCATGTATGA
GGAGACGCGTGCCGCGGGTTTCGGCAAGGAAGTGCGCCGCCGCATTTTGATCGGCACCTATGTGCTCTCGGCCGGCTATT
ATGATGCCTATTATGTCCGGGCACAGAAGATCCGCACCCTGATCAAACGCGATTTCGAACAGGTGTTCGAACAAGGGATC
GATGCGATCCTCACTCCGGCGACGCCGTCAGCCGCCTTCGGACTCGGTGAAAAAGGCGCCAAGGACCCGATCGAAATGTA
TCTCAACGATATTTTTACGGTGACGGTGAACATGGCCGGCCTGCCGGGCATTGCGGTGCCGGCCGGCGTTTCCGCCGAAG
GCTTGCCGCTCGGCCTGCAATTGATCGGCCGGCCTTTCGACGAGGAGACTTTGATCGCGCTCTCGGGGGTCATCGAGGCG
GCGGCACCACGTGTCTCTTATCCCTCTTCCTGGTGGGGTTGA

Upstream 100 bases:

>100_bases
GCCACGGAAGACCATTTCTTCGTCGTCCCCAAAGTTGTGGAATAGACCAGCCCTCCTGGTTTGAGCTGAATTTTAGATTG
CGAATGAACGGACGCGAAAC

Downstream 100 bases:

>100_bases
AGGAGGAGCACGGCGACGCAAAGCTATTGGGTTATGCGTCGCTGCCGCGAATGAGGTATAAGATTTCAAGAGACGAGCGT
GGGCTCGAACAAAGGGGTCC

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MTELTDLSLADARDALRQKKISAAELAMAHIEAVEKARALNAFLLETPERAQAMARASDEKIARGEAGPLEGIPLGIKDL
FCTEGVRTTAASHILENFVPAYESSITANLWRDGAVMLGKLNLDEFAMGSSNETSYFGPVVSPWRREGSTAPLTPGGSSG
GSAAAVAARLCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVVAFASSLDQAGPITRTVRDAAILLRSMAGYDPK
DTTSVNLPVPDYEAALGRSIKGMRIGIPKEYRLDAMPAEIGKLWDQGIEWMREAGAEPVEISLPHTRHALPAYYIVAPAE
ASSNLARYDGVRFGLRVPGKDIAGMYEETRAAGFGKEVRRRILIGTYVLSAGYYDAYYVRAQKIRTLIKRDFEQVFEQGI
DAILTPATPSAAFGLGEKGAKDPIEMYLNDIFTVTVNMAGLPGIAVPAGVSAEGLPLGLQLIGRPFDEETLIALSGVIEA
AAPRVSYPSSWWG

Sequences:

>Translated_493_residues
MTELTDLSLADARDALRQKKISAAELAMAHIEAVEKARALNAFLLETPERAQAMARASDEKIARGEAGPLEGIPLGIKDL
FCTEGVRTTAASHILENFVPAYESSITANLWRDGAVMLGKLNLDEFAMGSSNETSYFGPVVSPWRREGSTAPLTPGGSSG
GSAAAVAARLCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVVAFASSLDQAGPITRTVRDAAILLRSMAGYDPK
DTTSVNLPVPDYEAALGRSIKGMRIGIPKEYRLDAMPAEIGKLWDQGIEWMREAGAEPVEISLPHTRHALPAYYIVAPAE
ASSNLARYDGVRFGLRVPGKDIAGMYEETRAAGFGKEVRRRILIGTYVLSAGYYDAYYVRAQKIRTLIKRDFEQVFEQGI
DAILTPATPSAAFGLGEKGAKDPIEMYLNDIFTVTVNMAGLPGIAVPAGVSAEGLPLGLQLIGRPFDEETLIALSGVIEA
AAPRVSYPSSWWG
>Mature_492_residues
TELTDLSLADARDALRQKKISAAELAMAHIEAVEKARALNAFLLETPERAQAMARASDEKIARGEAGPLEGIPLGIKDLF
CTEGVRTTAASHILENFVPAYESSITANLWRDGAVMLGKLNLDEFAMGSSNETSYFGPVVSPWRREGSTAPLTPGGSSGG
SAAAVAARLCLGATATDTGGSIRQPAAFTGTVGIKPTYGRCSRWGVVAFASSLDQAGPITRTVRDAAILLRSMAGYDPKD
TTSVNLPVPDYEAALGRSIKGMRIGIPKEYRLDAMPAEIGKLWDQGIEWMREAGAEPVEISLPHTRHALPAYYIVAPAEA
SSNLARYDGVRFGLRVPGKDIAGMYEETRAAGFGKEVRRRILIGTYVLSAGYYDAYYVRAQKIRTLIKRDFEQVFEQGID
AILTPATPSAAFGLGEKGAKDPIEMYLNDIFTVTVNMAGLPGIAVPAGVSAEGLPLGLQLIGRPFDEETLIALSGVIEAA
APRVSYPSSWWG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=502, Percent_Identity=39.2430278884462, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI195972892, Length=514, Percent_Identity=22.9571984435798, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17543272, Length=452, Percent_Identity=36.5044247787611, Blast_Score=274, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17537465, Length=494, Percent_Identity=25.7085020242915, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17538252, Length=270, Percent_Identity=27.7777777777778, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=229, Percent_Identity=30.1310043668122, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71990152, Length=360, Percent_Identity=26.3888888888889, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=426, Percent_Identity=35.2112676056338, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6319685, Length=425, Percent_Identity=26.8235294117647, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648113, Length=454, Percent_Identity=37.8854625550661, Blast_Score=290, Evalue=1e-78,
Organism=Drosophila melanogaster, GI24644968, Length=505, Percent_Identity=23.3663366336634, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI45550774, Length=258, Percent_Identity=28.2945736434109, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652985, Length=494, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922090, Length=494, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652981, Length=494, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652983, Length=494, Percent_Identity=23.6842105263158, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648435, Length=258, Percent_Identity=28.2945736434109, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648437, Length=258, Percent_Identity=28.2945736434109, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356731, Length=491, Percent_Identity=23.8289205702648, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161078093, Length=266, Percent_Identity=27.8195488721804, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_BEII9 (B2IEA4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832991.1
- GeneID:   6199894
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1878
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52662; Mature: 52530

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 78-78 ACT_SITE 158-158 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELTDLSLADARDALRQKKISAAELAMAHIEAVEKARALNAFLLETPERAQAMARASDE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHH
KIARGEAGPLEGIPLGIKDLFCTEGVRTTAASHILENFVPAYESSITANLWRDGAVMLGK
HHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEE
LNLDEFAMGSSNETSYFGPVVSPWRREGSTAPLTPGGSSGGSAAAVAARLCLGATATDTG
CCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGVVAFASSLDQAGPITRTVRDAAILLRSMAGYDPK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DTTSVNLPVPDYEAALGRSIKGMRIGIPKEYRLDAMPAEIGKLWDQGIEWMREAGAEPVE
CCCEEECCCCCHHHHHCCCCCCEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
ISLPHTRHALPAYYIVAPAEASSNLARYDGVRFGLRVPGKDIAGMYEETRAAGFGKEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHH
RILIGTYVLSAGYYDAYYVRAQKIRTLIKRDFEQVFEQGIDAILTPATPSAAFGLGEKGA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCCCC
KDPIEMYLNDIFTVTVNMAGLPGIAVPAGVSAEGLPLGLQLIGRPFDEETLIALSGVIEA
CCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
AAPRVSYPSSWWG
CCCCCCCCCCCCC
>Mature Secondary Structure 
TELTDLSLADARDALRQKKISAAELAMAHIEAVEKARALNAFLLETPERAQAMARASDE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHH
KIARGEAGPLEGIPLGIKDLFCTEGVRTTAASHILENFVPAYESSITANLWRDGAVMLGK
HHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCEEEEE
LNLDEFAMGSSNETSYFGPVVSPWRREGSTAPLTPGGSSGGSAAAVAARLCLGATATDTG
CCHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCC
GSIRQPAAFTGTVGIKPTYGRCSRWGVVAFASSLDQAGPITRTVRDAAILLRSMAGYDPK
CCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCC
DTTSVNLPVPDYEAALGRSIKGMRIGIPKEYRLDAMPAEIGKLWDQGIEWMREAGAEPVE
CCCEEECCCCCHHHHHCCCCCCEEECCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEE
ISLPHTRHALPAYYIVAPAEASSNLARYDGVRFGLRVPGKDIAGMYEETRAAGFGKEVRR
EECCCCCCCCCEEEEEECCCCCCCHHHHCCEEEEEECCCHHHHHHHHHHHHHCCCHHHHH
RILIGTYVLSAGYYDAYYVRAQKIRTLIKRDFEQVFEQGIDAILTPATPSAAFGLGEKGA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHCCCCCCCC
KDPIEMYLNDIFTVTVNMAGLPGIAVPAGVSAEGLPLGLQLIGRPFDEETLIALSGVIEA
CCHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHH
AAPRVSYPSSWWG
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA