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The map label for this gene is smpB

Identifier: 182678836

GI number: 182678836

Start: 2129732

End: 2130208

Strand: Reverse

Name: smpB

Synonym: Bind_1869

Alternate gene names: 182678836

Gene position: 2130208-2129732 (Counterclockwise)

Preceding gene: 182678837

Following gene: 182678835

Centisome position: 51.08

GC content: 56.81

Gene sequence:

>477_bases
GTGGCCGCAAAGAAACCAAGCGCGTTCAAGATCGCCGCGGAAAACCGCAAGGCGCGCTATAATTATGAAATTGGCGAGAC
TTTCGAGGCGGGGCTCATGCTGACCGGCACCGAGGTCAAATCCTTGCGCACCGGCAAGGCGACCATTGCCGAGAGCTATG
CCTCTGTGGATCGGGAGGGCGAGGTGTTTCTCGTCAACGCGACCATCCCCGAATATCTCGAGGCCAATCGCTTCAACCAT
GCGCCAAAACGGCCGCGCAAACTTCTGTTGCATGGGCGCGAGATCGCCAAGCTTTCGCAAGGGGTCGAGCGTCAAGGCAT
GACCATTGTGCCGCTGAAAATTTACTTCAATGATCGGGGCAGGGCGAAGATTGAAATCGCCTTGGCGCGCGGCAAACAAT
TGCACGACAAGCGCGAGACCGAAAAGAAACGCGATTGGAACAAGGAAAAGGGCCGGCTGCTGCGCGACAAACATTGA

Upstream 100 bases:

>100_bases
GAGATCAGGCTGCCCCTCGTCTCCGCCTCGGTCAATGCCCAGGCCGTGATCCGCGCTGCCATGGTGCATGCCGGTCTCAT
TACAGAATAAGTGTGCTTGA

Downstream 100 bases:

>100_bases
GATCGGCCTTCCTCATCTTCTTTGTCTTCCCTGTTTTCGGCATCATCTCTCATGTCTCACAATACATTCGGCCATCTCTT
TCGCGTGACGACCTTTGGGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MAAKKPSAFKIAAENRKARYNYEIGETFEAGLMLTGTEVKSLRTGKATIAESYASVDREGEVFLVNATIPEYLEANRFNH
APKRPRKLLLHGREIAKLSQGVERQGMTIVPLKIYFNDRGRAKIEIALARGKQLHDKRETEKKRDWNKEKGRLLRDKH

Sequences:

>Translated_158_residues
MAAKKPSAFKIAAENRKARYNYEIGETFEAGLMLTGTEVKSLRTGKATIAESYASVDREGEVFLVNATIPEYLEANRFNH
APKRPRKLLLHGREIAKLSQGVERQGMTIVPLKIYFNDRGRAKIEIALARGKQLHDKRETEKKRDWNKEKGRLLRDKH
>Mature_157_residues
AAKKPSAFKIAAENRKARYNYEIGETFEAGLMLTGTEVKSLRTGKATIAESYASVDREGEVFLVNATIPEYLEANRFNHA
PKRPRKLLLHGREIAKLSQGVERQGMTIVPLKIYFNDRGRAKIEIALARGKQLHDKRETEKKRDWNKEKGRLLRDKH

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=44.5859872611465, Blast_Score=122, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_BEII9 (B2IDQ9)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832982.1
- GeneID:   6201328
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1869
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18092; Mature: 17961

Theoretical pI: Translated: 10.68; Mature: 10.68

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKKPSAFKIAAENRKARYNYEIGETFEAGLMLTGTEVKSLRTGKATIAESYASVDREG
CCCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEECCHHHHHHCCCHHHHHHHHHCCCCC
EVFLVNATIPEYLEANRFNHAPKRPRKLLLHGREIAKLSQGVERQGMTIVPLKIYFNDRG
CEEEEECCCHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHCHHHCCCEEEEEEEEECCCC
RAKIEIALARGKQLHDKRETEKKRDWNKEKGRLLRDKH
CEEEEEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCC
>Mature Secondary Structure 
AAKKPSAFKIAAENRKARYNYEIGETFEAGLMLTGTEVKSLRTGKATIAESYASVDREG
CCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEECCHHHHHHCCCHHHHHHHHHCCCCC
EVFLVNATIPEYLEANRFNHAPKRPRKLLLHGREIAKLSQGVERQGMTIVPLKIYFNDRG
CEEEEECCCHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHCHHHCCCEEEEEEEEECCCC
RAKIEIALARGKQLHDKRETEKKRDWNKEKGRLLRDKH
CEEEEEEEECCCHHHHHHHHHHHHCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA