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The map label for this gene is pyk [H]

Identifier: 182678831

GI number: 182678831

Start: 2124129

End: 2125541

Strand: Direct

Name: pyk [H]

Synonym: Bind_1864

Alternate gene names: 182678831

Gene position: 2124129-2125541 (Clockwise)

Preceding gene: 182678829

Following gene: 182678832

Centisome position: 50.94

GC content: 61.08

Gene sequence:

>1413_bases
ATGAAACGCCTTCGCCGTTGCAAGATCGTCGCGACACTCGGCCCCGCTTCCTCAGACAAGGCCACGATCGAGGCTCTGTT
CAAAGCTGGCGCCGATGTCTTCCGTATCAATATGAGCCATACGTCTCACGACAACTTGCGCGAGTACGTTCGCGTCATTC
GCGAAATCGAGGCAGATAGCGGCCGCCCGATCGCCATTCTCGCCGATCTGCAGGGACCCAAATTGCGGCTCGGTGAATTT
GCGAGTGGTGGCGCAGATCTCGTCAAGGGAGCGACCTTCACCCTTGATCAGCAGGAGAGCCTTGGTGATGCGCAAAGGGT
GCATCTCCCACATCCGGAAATTCTCAAGGCCCTCAAACCGGGCGACACTGTCCTGATCGATGACGGCAAGGTTCGGCTGC
ATGTTATCGAGGCCGATGCGAGCCACGCCGTTACTCTTGTCGATGTCGCGGGCCGTGTCTCGAACCGCAAGGGTGTGAGC
CTTCCGGATACCGAGATCGCCGTTTCCGCCATGACCTCAAAGGATCACTCGGATCTCGCGGCGGCGCTCAATGCCGAGGT
CGACTGGATCGCCTTGTCCTTCGTGCAACGGGGCGATGATGTCGCCGAGGTCAAAAAGATCGCCGGCGACCGAGCCTCGG
TGATCGCCAAGATCGAAAAGCCGCAAGCTATAACGAGGCTCGATGAAATCCTCGAACTGTCCGATGGATTGATGGTGGCG
CGTGGCGATCTCGGGGTGGAAATGCCGCTCGAGAAAGTGCCTGGCTTGCAAAAACGCATCACCCGCGCCGCGCGCCGCCT
GGGCAAACCCGTGATCGTCGCGACGCAAATGCTCGAATCGATGATCAATCTGCCCGTGCCAACGCGCGCCGAAGTCTCCG
ACGTCGCGACCGCTGTTTTCGATGGCGCTGATGCGGTCATGCTCTCCGCGGAAAGTGCTGCTGGGCAATATCCGGTCGAG
GCGGTCACGACGATGAACCGCATCGCGGAGGAAGTTGAAAAAGACGCCGTTTATGCGTCGATCCTCAGTGCTCAGCACGC
TGTGCCGGAGAAGACGGCCGCCGACGCGATCGCCATCGCCGCCCGGGAAATCACCGAGACCCTGGACGCCACGGTTATCG
TCGCCTGGACTTTCTCCGGGTCGACGGCGCTGCGCATCGCGCGTGAACGCTCTCCGGCTCCGCTCATCGCTTTGACGCCG
AAACAGCAGACCGCCCGCCGGCTCGTGCTCGCCTGGGGTGTGCATGCCGTTGTGACCGAGGACGCCCATGATATCGACGA
TATGACCCAACGCGCGAGCCGCTTCGCGCTGCAGGAAGGCTTCGCCACGAAGAATGATCGGATCGTGATCATTGTCGGTG
TCCCGTTCGGAACGCCAGGCGCCACCAATCTCATCCGCCTCGCCCGCGTTTAA

Upstream 100 bases:

>100_bases
ACTTGCCTGGACTTGTCTTCAGCGGAAGAGAGCAACGACCGTATCCACTTGAGAAAAACGCCAGGCTCACTCTTGAAGCA
GCCCGAAAGGAAACGCCAAG

Downstream 100 bases:

>100_bases
AGGAACGGCCCATGAAAAAAATCATGCTGGTATCGGTCACCGCGCTGAGCCTTGTGGCGAGCAGTCATAAGGCACCCGCC
GAGCCGCAGGACACGAAAAA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKRLRRCKIVATLGPASSDKATIEALFKAGADVFRINMSHTSHDNLREYVRVIREIEADSGRPIAILADLQGPKLRLGEF
ASGGADLVKGATFTLDQQESLGDAQRVHLPHPEILKALKPGDTVLIDDGKVRLHVIEADASHAVTLVDVAGRVSNRKGVS
LPDTEIAVSAMTSKDHSDLAAALNAEVDWIALSFVQRGDDVAEVKKIAGDRASVIAKIEKPQAITRLDEILELSDGLMVA
RGDLGVEMPLEKVPGLQKRITRAARRLGKPVIVATQMLESMINLPVPTRAEVSDVATAVFDGADAVMLSAESAAGQYPVE
AVTTMNRIAEEVEKDAVYASILSAQHAVPEKTAADAIAIAAREITETLDATVIVAWTFSGSTALRIARERSPAPLIALTP
KQQTARRLVLAWGVHAVVTEDAHDIDDMTQRASRFALQEGFATKNDRIVIIVGVPFGTPGATNLIRLARV

Sequences:

>Translated_470_residues
MKRLRRCKIVATLGPASSDKATIEALFKAGADVFRINMSHTSHDNLREYVRVIREIEADSGRPIAILADLQGPKLRLGEF
ASGGADLVKGATFTLDQQESLGDAQRVHLPHPEILKALKPGDTVLIDDGKVRLHVIEADASHAVTLVDVAGRVSNRKGVS
LPDTEIAVSAMTSKDHSDLAAALNAEVDWIALSFVQRGDDVAEVKKIAGDRASVIAKIEKPQAITRLDEILELSDGLMVA
RGDLGVEMPLEKVPGLQKRITRAARRLGKPVIVATQMLESMINLPVPTRAEVSDVATAVFDGADAVMLSAESAAGQYPVE
AVTTMNRIAEEVEKDAVYASILSAQHAVPEKTAADAIAIAAREITETLDATVIVAWTFSGSTALRIARERSPAPLIALTP
KQQTARRLVLAWGVHAVVTEDAHDIDDMTQRASRFALQEGFATKNDRIVIIVGVPFGTPGATNLIRLARV
>Mature_470_residues
MKRLRRCKIVATLGPASSDKATIEALFKAGADVFRINMSHTSHDNLREYVRVIREIEADSGRPIAILADLQGPKLRLGEF
ASGGADLVKGATFTLDQQESLGDAQRVHLPHPEILKALKPGDTVLIDDGKVRLHVIEADASHAVTLVDVAGRVSNRKGVS
LPDTEIAVSAMTSKDHSDLAAALNAEVDWIALSFVQRGDDVAEVKKIAGDRASVIAKIEKPQAITRLDEILELSDGLMVA
RGDLGVEMPLEKVPGLQKRITRAARRLGKPVIVATQMLESMINLPVPTRAEVSDVATAVFDGADAVMLSAESAAGQYPVE
AVTTMNRIAEEVEKDAVYASILSAQHAVPEKTAADAIAIAAREITETLDATVIVAWTFSGSTALRIARERSPAPLIALTP
KQQTARRLVLAWGVHAVVTEDAHDIDDMTQRASRFALQEGFATKNDRIVIIVGVPFGTPGATNLIRLARV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=37.8323108384458, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=37.3983739837398, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=37.3983739837398, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI10835121, Length=487, Percent_Identity=37.9876796714579, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI32967597, Length=487, Percent_Identity=37.9876796714579, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=36.9337979094077, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=36.9337979094077, Blast_Score=175, Evalue=7e-44,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=31.6455696202532, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=31.6455696202532, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=30.8411214953271, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=39.5833333333333, Blast_Score=299, Evalue=2e-82,
Organism=Escherichia coli, GI1787965, Length=465, Percent_Identity=38.7096774193548, Blast_Score=292, Evalue=4e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=35.0622406639004, Blast_Score=285, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=36.0824742268041, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=36.0824742268041, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=36.0824742268041, Blast_Score=241, Evalue=4e-64,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=36.0824742268041, Blast_Score=241, Evalue=4e-64,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=34.156378600823, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6324923, Length=489, Percent_Identity=33.7423312883436, Blast_Score=236, Evalue=8e-63,
Organism=Drosophila melanogaster, GI28571814, Length=494, Percent_Identity=36.0323886639676, Blast_Score=281, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24648964, Length=494, Percent_Identity=36.0323886639676, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI24581235, Length=483, Percent_Identity=34.7826086956522, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=35.7843137254902, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24646914, Length=283, Percent_Identity=36.7491166077738, Blast_Score=166, Evalue=3e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50407; Mature: 50407

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLRRCKIVATLGPASSDKATIEALFKAGADVFRINMSHTSHDNLREYVRVIREIEADS
CCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
GRPIAILADLQGPKLRLGEFASGGADLVKGATFTLDQQESLGDAQRVHLPHPEILKALKP
CCCEEEEEECCCCCEECCCCCCCCHHHHCCCEEEECCHHHCCCCCEEECCCHHHHHHCCC
GDTVLIDDGKVRLHVIEADASHAVTLVDVAGRVSNRKGVSLPDTEIAVSAMTSKDHSDLA
CCEEEEECCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
AALNAEVDWIALSFVQRGDDVAEVKKIAGDRASVIAKIEKPQAITRLDEILELSDGLMVA
HHHCCCCHHEEHHHHHCCCCHHHHHHHCCCHHHHEEECCCCHHHHHHHHHHHHCCCEEEE
RGDLGVEMPLEKVPGLQKRITRAARRLGKPVIVATQMLESMINLPVPTRAEVSDVATAVF
ECCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DGADAVMLSAESAAGQYPVEAVTTMNRIAEEVEKDAVYASILSAQHAVPEKTAADAIAIA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AREITETLDATVIVAWTFSGSTALRIARERSPAPLIALTPKQQTARRLVLAWGVHAVVTE
HHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHCCEEEEEC
DAHDIDDMTQRASRFALQEGFATKNDRIVIIVGVPFGTPGATNLIRLARV
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MKRLRRCKIVATLGPASSDKATIEALFKAGADVFRINMSHTSHDNLREYVRVIREIEADS
CCCHHHEEEEEEECCCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHCCCC
GRPIAILADLQGPKLRLGEFASGGADLVKGATFTLDQQESLGDAQRVHLPHPEILKALKP
CCCEEEEEECCCCCEECCCCCCCCHHHHCCCEEEECCHHHCCCCCEEECCCHHHHHHCCC
GDTVLIDDGKVRLHVIEADASHAVTLVDVAGRVSNRKGVSLPDTEIAVSAMTSKDHSDLA
CCEEEEECCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCHHHHH
AALNAEVDWIALSFVQRGDDVAEVKKIAGDRASVIAKIEKPQAITRLDEILELSDGLMVA
HHHCCCCHHEEHHHHHCCCCHHHHHHHCCCHHHHEEECCCCHHHHHHHHHHHHCCCEEEE
RGDLGVEMPLEKVPGLQKRITRAARRLGKPVIVATQMLESMINLPVPTRAEVSDVATAVF
ECCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
DGADAVMLSAESAAGQYPVEAVTTMNRIAEEVEKDAVYASILSAQHAVPEKTAADAIAIA
CCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AREITETLDATVIVAWTFSGSTALRIARERSPAPLIALTPKQQTARRLVLAWGVHAVVTE
HHHHHHHCCCEEEEEEEECCCEEEEEECCCCCCCEEEECCCHHHHHHHHHHHCCEEEEEC
DAHDIDDMTQRASRFALQEGFATKNDRIVIIVGVPFGTPGATNLIRLARV
CCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA