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The map label for this gene is ppdK [H]

Identifier: 182678780

GI number: 182678780

Start: 2054620

End: 2057298

Strand: Reverse

Name: ppdK [H]

Synonym: Bind_1809

Alternate gene names: 182678780

Gene position: 2057298-2054620 (Counterclockwise)

Preceding gene: 182678781

Following gene: 182678779

Centisome position: 49.33

GC content: 60.1

Gene sequence:

>2679_bases
ATGGGGAAATGGGTTTATACATTCGGAGACGGTAAGGCCGAGGGAGCGGGCGGAATGCGCAATCTGCTCGGCGGCAAGGG
TGCCAATCTTGCCGAAATGGCCAATCTCGGCCTGCCGGTTCCGCCCGGCTTCACCATTACTACCGAGGTTTGCACCGCCT
TTTACGAAAACAACAAGGCCTATCCCAGCGAACTCGCCGGTGAGGTCACCAATGCTTTGTCGCATGTGGCCAAATTGACA
GGCCGGATTTTCGGCGATGATCAAAATCCGCTTCTTGTCTCGGTTCGCTCCGGTGCCCGCGCTTCCATGCCGGGCATGAT
GGATACCGTCCTCAATCTCGGTCTCAATGACACGACCGTTCTGGCGCTGGCGAAAAACGCCGACGACGAGCGTTTCGCCT
ATGATTCCTATCGCCGTTTCATCCAGATGTATTCCAATGTGGTGCTTGGCATTGAGCATCATCATTTCGAGGAAATCCTG
GAGCACTACAAGGAATCCAAGGGATTTGTGCTCGACACGGATCTCAGCGCGGCGGACTGGCAGGTCATCATCACCGGCTA
TAAGAAGCAGGTGCAGGACACGCTCGGCCATCCGTTCCCGCAGGACCCGCAGGAACAATTGTGGGGCGCGATCGGCGCTG
TCTTCGGCTCCTGGATGAATGCCCGTGCCATCACTTATCGTCGCCTGCACGATATTCCGGCGAGCTGGGGCACGGCGGTC
ACCGTCCAGGCCATGGTGTTCGGCAATATGGGTGACACCTCGGCCACTGGCGTTGCCTTTACCCGCAACCCGTCCACGGG
CGTCAAGGAGCTCTATGGCGAGTTCCTAATCAACGCCCAAGGCGAGGATGTCGTCGCTGGTATCCGCACGCCGCAGAATA
TTACTGAGGAGGCGCGCATCGGCGCCGGCTCCGACAAACCCTCGATGGAAACCGCCCTGCCGGAGGTTTTCGCGGAATTC
GTGCGCACGACCAACCTGCTCGAAAAGCACTACCGCGACATGCAGGATATGGAATTCACGGTCGAGCGCGGCAAATTGTG
GATGCTGCAGACCCGTAACGGCAAGCGGACCGGCAAGGCCTCGCTGCGGATTGCCGTCGATATGGCCAATGAAGGCTTGA
TCACCAAGGAAGAAGCGGTCGGCCGCGTCGATCCGGCTTCGCTCGATCAATTGCTGCATCCGACCATCGACCCCAAGGCC
GAACTCACCATTGTCGCGACGGGCCTGCCGGCTTCTCCCGGTGCGGCCTCTGGAGAAATCGTTTTCACCTCTGATGAAGC
GGAGCATTTGAAGAGCGCCGGTCATAAGGTGATCTTGGTCCGCGTCGAGACCTCGCCCGAAGATATTCACGGCATGCATG
CGTCGGAAGGCATTCTGACAACGCGTGGCGGCATGACCTCGCATGCGGCGGTCGTCGCGCGTGGCATGGGCAAGCCCTGC
GTCTCCGGGGCCGGCAGCGTGCGCGTCGATTATGCGCAAGGCACGATGACCGCTTCGGGCGTTACCTTCAAGAAGGGCGA
CATGATCACCATCGACGGGGGAACCGGTCAGGTGATCAAGGGCGCGACGCAAATGCTGCAGCCTGAACTTTCGGGTGAGT
TTGCTGTCCTGATGGAATGGGCCGACAAGGCGCGCCGCATGAATGTTCGTGCCAATGCCGAGACCCCACAAGACGCTCGC
GTTGCGCGCAAGTTCGGCGCCGAGGGGATCGGCCTTTGCCGCACCGAGCACATGTTCTTTGAGTCCGATCGTATCGTCGC
CGTGCGCGAAATGATCCTTGCCGAGAACGAAGCCGGCCGCCGCGCGGCTCTTGCCAAGCTCCTCCCGATCCAGCGTGGTG
ATTTCGCTGAATTGTTCGAGATCATGTCCGGCCTGCCGGTCACGATCCGTCTGCTCGATCCGCCGCTGCATGAATTTTTG
CCCCAGACCGAAGCGGAAATCGCCGAGGTTGCCAAATCGCTCGACGTCTCGGCCGAGAGGCTGAAGCATCGCGCGGCGGA
ACTGCACGAATTCAACCCGATGCTCGGGTTCCGCGGCTGCCGCCTGGCAATCGCCTTCCCGGAAATCGCCGAAATGCAGG
CGCGCGCTATTTTCGAAGGCGCGGTCGAGGCGAAGAAAAAGACTGGCCAGACTGTCCAGCCGGAAGTCATGGTGCCCTTG
ATCGTGAGCAAGGCCGAATTCGATCTCGTCAAGGATCGGATCGATGCCATGGCTAAGCTGGTTGCCGAGGAAACCGGGGA
GACGATCCCCTATAAAGTCGGCACGATGATCGAGACGCCGCGCGCAGCCTTGCGGGCCGGGGAGATCGCCGAAACGGCGG
AATTCTTCTCCTTCGGGACGAATGACCTGACGCAGACATGCCTTGGCATTTCGCGTGACGATGCTGGGTCCTTCCTTGGC
ACCTATACGGCCAAGGGCATTCTGCCGAATGACCCCTTTGTGTCGATTGATCAGGAAGGCGTGGGTGAGTTGGTCAAGAT
CGGTGTCTCTCGTGGCCGCGAGACGCGCGATGCCATCAAGCTCGGCATTTGTGGCGAGCATGGTGGCGATCCGTCCTCAA
TCGGCTTTTGCGAAAAGGTTGGGCTCGATTACGTCTCTTGCTCTCCGTTCCGGGTGCCGATCGCACGGCTTGCAGCCGCT
CAGGCCGCGTTGAATCAGGCGGCGGCTAGCCAAGCCTGA

Upstream 100 bases:

>100_bases
CTTCGAACATGAGAAGACCCTGATCAATCGTTCACGAATGCAAGAGATTCGTGAGGGCATACCTTGCACGATTCACCTTT
GATAAAGGGAAGATTCCGAA

Downstream 100 bases:

>100_bases
TAATCGCAGGCAGGATGATCCGATGAATTGAAAATCCGCCCGGTAGCCCCGGGCGGATTTTTTGCGCTTTAGAGCATCGG
ACTTTCAAATTGATCATCTC

Product: pyruvate phosphate dikinase

Products: NA

Alternate protein names: Pyruvate, orthophosphate dikinase [H]

Number of amino acids: Translated: 892; Mature: 891

Protein sequence:

>892_residues
MGKWVYTFGDGKAEGAGGMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTAFYENNKAYPSELAGEVTNALSHVAKLT
GRIFGDDQNPLLVSVRSGARASMPGMMDTVLNLGLNDTTVLALAKNADDERFAYDSYRRFIQMYSNVVLGIEHHHFEEIL
EHYKESKGFVLDTDLSAADWQVIITGYKKQVQDTLGHPFPQDPQEQLWGAIGAVFGSWMNARAITYRRLHDIPASWGTAV
TVQAMVFGNMGDTSATGVAFTRNPSTGVKELYGEFLINAQGEDVVAGIRTPQNITEEARIGAGSDKPSMETALPEVFAEF
VRTTNLLEKHYRDMQDMEFTVERGKLWMLQTRNGKRTGKASLRIAVDMANEGLITKEEAVGRVDPASLDQLLHPTIDPKA
ELTIVATGLPASPGAASGEIVFTSDEAEHLKSAGHKVILVRVETSPEDIHGMHASEGILTTRGGMTSHAAVVARGMGKPC
VSGAGSVRVDYAQGTMTASGVTFKKGDMITIDGGTGQVIKGATQMLQPELSGEFAVLMEWADKARRMNVRANAETPQDAR
VARKFGAEGIGLCRTEHMFFESDRIVAVREMILAENEAGRRAALAKLLPIQRGDFAELFEIMSGLPVTIRLLDPPLHEFL
PQTEAEIAEVAKSLDVSAERLKHRAAELHEFNPMLGFRGCRLAIAFPEIAEMQARAIFEGAVEAKKKTGQTVQPEVMVPL
IVSKAEFDLVKDRIDAMAKLVAEETGETIPYKVGTMIETPRAALRAGEIAETAEFFSFGTNDLTQTCLGISRDDAGSFLG
TYTAKGILPNDPFVSIDQEGVGELVKIGVSRGRETRDAIKLGICGEHGGDPSSIGFCEKVGLDYVSCSPFRVPIARLAAA
QAALNQAAASQA

Sequences:

>Translated_892_residues
MGKWVYTFGDGKAEGAGGMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTAFYENNKAYPSELAGEVTNALSHVAKLT
GRIFGDDQNPLLVSVRSGARASMPGMMDTVLNLGLNDTTVLALAKNADDERFAYDSYRRFIQMYSNVVLGIEHHHFEEIL
EHYKESKGFVLDTDLSAADWQVIITGYKKQVQDTLGHPFPQDPQEQLWGAIGAVFGSWMNARAITYRRLHDIPASWGTAV
TVQAMVFGNMGDTSATGVAFTRNPSTGVKELYGEFLINAQGEDVVAGIRTPQNITEEARIGAGSDKPSMETALPEVFAEF
VRTTNLLEKHYRDMQDMEFTVERGKLWMLQTRNGKRTGKASLRIAVDMANEGLITKEEAVGRVDPASLDQLLHPTIDPKA
ELTIVATGLPASPGAASGEIVFTSDEAEHLKSAGHKVILVRVETSPEDIHGMHASEGILTTRGGMTSHAAVVARGMGKPC
VSGAGSVRVDYAQGTMTASGVTFKKGDMITIDGGTGQVIKGATQMLQPELSGEFAVLMEWADKARRMNVRANAETPQDAR
VARKFGAEGIGLCRTEHMFFESDRIVAVREMILAENEAGRRAALAKLLPIQRGDFAELFEIMSGLPVTIRLLDPPLHEFL
PQTEAEIAEVAKSLDVSAERLKHRAAELHEFNPMLGFRGCRLAIAFPEIAEMQARAIFEGAVEAKKKTGQTVQPEVMVPL
IVSKAEFDLVKDRIDAMAKLVAEETGETIPYKVGTMIETPRAALRAGEIAETAEFFSFGTNDLTQTCLGISRDDAGSFLG
TYTAKGILPNDPFVSIDQEGVGELVKIGVSRGRETRDAIKLGICGEHGGDPSSIGFCEKVGLDYVSCSPFRVPIARLAAA
QAALNQAAASQA
>Mature_891_residues
GKWVYTFGDGKAEGAGGMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTAFYENNKAYPSELAGEVTNALSHVAKLTG
RIFGDDQNPLLVSVRSGARASMPGMMDTVLNLGLNDTTVLALAKNADDERFAYDSYRRFIQMYSNVVLGIEHHHFEEILE
HYKESKGFVLDTDLSAADWQVIITGYKKQVQDTLGHPFPQDPQEQLWGAIGAVFGSWMNARAITYRRLHDIPASWGTAVT
VQAMVFGNMGDTSATGVAFTRNPSTGVKELYGEFLINAQGEDVVAGIRTPQNITEEARIGAGSDKPSMETALPEVFAEFV
RTTNLLEKHYRDMQDMEFTVERGKLWMLQTRNGKRTGKASLRIAVDMANEGLITKEEAVGRVDPASLDQLLHPTIDPKAE
LTIVATGLPASPGAASGEIVFTSDEAEHLKSAGHKVILVRVETSPEDIHGMHASEGILTTRGGMTSHAAVVARGMGKPCV
SGAGSVRVDYAQGTMTASGVTFKKGDMITIDGGTGQVIKGATQMLQPELSGEFAVLMEWADKARRMNVRANAETPQDARV
ARKFGAEGIGLCRTEHMFFESDRIVAVREMILAENEAGRRAALAKLLPIQRGDFAELFEIMSGLPVTIRLLDPPLHEFLP
QTEAEIAEVAKSLDVSAERLKHRAAELHEFNPMLGFRGCRLAIAFPEIAEMQARAIFEGAVEAKKKTGQTVQPEVMVPLI
VSKAEFDLVKDRIDAMAKLVAEETGETIPYKVGTMIETPRAALRAGEIAETAEFFSFGTNDLTQTCLGISRDDAGSFLGT
YTAKGILPNDPFVSIDQEGVGELVKIGVSRGRETRDAIKLGICGEHGGDPSSIGFCEKVGLDYVSCSPFRVPIARLAAAQ
AALNQAAASQA

Specific function: Catalyzes the reversible phosphorylation of pyruvate and phosphate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=463, Percent_Identity=25.7019438444924, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI48994992, Length=464, Percent_Identity=25.4310344827586, Blast_Score=90, Evalue=7e-19,
Organism=Escherichia coli, GI1789193, Length=431, Percent_Identity=24.5939675174014, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR010121
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.1 [H]

Molecular weight: Translated: 96273; Mature: 96142

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKWVYTFGDGKAEGAGGMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTAFYENNKA
CCCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCC
YPSELAGEVTNALSHVAKLTGRIFGDDQNPLLVSVRSGARASMPGMMDTVLNLGLNDTTV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEE
LALAKNADDERFAYDSYRRFIQMYSNVVLGIEHHHFEEILEHYKESKGFVLDTDLSAADW
EEEECCCCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCC
QVIITGYKKQVQDTLGHPFPQDPQEQLWGAIGAVFGSWMNARAITYRRLHDIPASWGTAV
EEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
TVQAMVFGNMGDTSATGVAFTRNPSTGVKELYGEFLINAQGEDVVAGIRTPQNITEEARI
EEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHC
GAGSDKPSMETALPEVFAEFVRTTNLLEKHYRDMQDMEFTVERGKLWMLQTRNGKRTGKA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCCCCCE
SLRIAVDMANEGLITKEEAVGRVDPASLDQLLHPTIDPKAELTIVATGLPASPGAASGEI
EEEEEEEECCCCCEEHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCE
VFTSDEAEHLKSAGHKVILVRVETSPEDIHGMHASEGILTTRGGMTSHAAVVARGMGKPC
EEECCHHHHHHHCCCEEEEEEEECCCHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCCCC
VSGAGSVRVDYAQGTMTASGVTFKKGDMITIDGGTGQVIKGATQMLQPELSGEFAVLMEW
CCCCCCEEEEECCCCEEECCEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHH
ADKARRMNVRANAETPQDARVARKFGAEGIGLCRTEHMFFESDRIVAVREMILAENEAGR
HHHHHEEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHEEECCCCHHHHHHHHHHCCCCCH
RAALAKLLPIQRGDFAELFEIMSGLPVTIRLLDPPLHEFLPQTEAEIAEVAKSLDVSAER
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCHHHH
LKHRAAELHEFNPMLGFRGCRLAIAFPEIAEMQARAIFEGAVEAKKKTGQTVQPEVMVPL
HHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
IVSKAEFDLVKDRIDAMAKLVAEETGETIPYKVGTMIETPRAALRAGEIAETAEFFSFGT
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEECCCHHHHHHCCHHHHHHHHHCCC
NDLTQTCLGISRDDAGSFLGTYTAKGILPNDPFVSIDQEGVGELVKIGVSRGRETRDAIK
HHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHHEE
LGICGEHGGDPSSIGFCEKVGLDYVSCSPFRVPIARLAAAQAALNQAAASQA
EEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GKWVYTFGDGKAEGAGGMRNLLGGKGANLAEMANLGLPVPPGFTITTEVCTAFYENNKA
CCEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCC
YPSELAGEVTNALSHVAKLTGRIFGDDQNPLLVSVRSGARASMPGMMDTVLNLGLNDTTV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEE
LALAKNADDERFAYDSYRRFIQMYSNVVLGIEHHHFEEILEHYKESKGFVLDTDLSAADW
EEEECCCCCHHHHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHCCCEEEECCCCCCCC
QVIITGYKKQVQDTLGHPFPQDPQEQLWGAIGAVFGSWMNARAITYRRLHDIPASWGTAV
EEEEECHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
TVQAMVFGNMGDTSATGVAFTRNPSTGVKELYGEFLINAQGEDVVAGIRTPQNITEEARI
EEEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHEECCCCCCEEEECCCCCCHHHHHHC
GAGSDKPSMETALPEVFAEFVRTTNLLEKHYRDMQDMEFTVERGKLWMLQTRNGKRTGKA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCEEEEEECCCCCCCCE
SLRIAVDMANEGLITKEEAVGRVDPASLDQLLHPTIDPKAELTIVATGLPASPGAASGEI
EEEEEEEECCCCCEEHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCE
VFTSDEAEHLKSAGHKVILVRVETSPEDIHGMHASEGILTTRGGMTSHAAVVARGMGKPC
EEECCHHHHHHHCCCEEEEEEEECCCHHHCCCCCCCCEEEECCCCCHHHHHHHHCCCCCC
VSGAGSVRVDYAQGTMTASGVTFKKGDMITIDGGTGQVIKGATQMLQPELSGEFAVLMEW
CCCCCCEEEEECCCCEEECCEEEECCCEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHH
ADKARRMNVRANAETPQDARVARKFGAEGIGLCRTEHMFFESDRIVAVREMILAENEAGR
HHHHHEEEEEECCCCCHHHHHHHHHCCCCCCEEEEHHEEECCCCHHHHHHHHHHCCCCCH
RAALAKLLPIQRGDFAELFEIMSGLPVTIRLLDPPLHEFLPQTEAEIAEVAKSLDVSAER
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCCHHHH
LKHRAAELHEFNPMLGFRGCRLAIAFPEIAEMQARAIFEGAVEAKKKTGQTVQPEVMVPL
HHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
IVSKAEFDLVKDRIDAMAKLVAEETGETIPYKVGTMIETPRAALRAGEIAETAEFFSFGT
HHCHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEECCCHHHHHHCCHHHHHHHHHCCC
NDLTQTCLGISRDDAGSFLGTYTAKGILPNDPFVSIDQEGVGELVKIGVSRGRETRDAIK
HHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCCHHHEE
LGICGEHGGDPSSIGFCEKVGLDYVSCSPFRVPIARLAAAQAALNQAAASQA
EEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430; 9168612 [H]