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The map label for this gene is nifK [H]

Identifier: 182678582

GI number: 182678582

Start: 1814247

End: 1815806

Strand: Direct

Name: nifK [H]

Synonym: Bind_1607

Alternate gene names: 182678582

Gene position: 1814247-1815806 (Clockwise)

Preceding gene: 182678580

Following gene: 182678584

Centisome position: 43.51

GC content: 55.77

Gene sequence:

>1560_bases
ATGCCGCAAAACGCCCAATCGGTTCTCGACCATTTCGATCTCTTCAAGCAGCCCGAATATCGGGAAATGTTTGCCAATAA
GAAGAAATACTTTGAAAACGCGGTCGCCGAGGTTGAAGTGGACCGTATCCGCGAATGGATGAAGACGCCGGAATATCAGG
AGAAGAATTTTGCTCGCGAGGCCCTGACGGTCAATCCGGCCAAGGCCTGTCAGCCGCTCGGCGCTGTCTTCTGCAGCAGC
GGATTCATGGGCGCACTCTCGTTTGTGCATGGATCGCAAGGCTGCGTCGCCTATTACCGCAGCCATCTGTCGCGGCATTT
CAAGGAGCCCTGCTCCTGTGTCTCTTCCTCGATGACCGAGGATGCAGCGGTGTTCGGCGGTCTCACCAATATGATCGATG
GTCTCGCCAATACCTATAATCTCTATCGCCCGGCAATGATCTCGGTCTCGACCACCTGCATGGCCGAGGTGATCGGCGAC
GATCTCAACGCCTTCATCAAAACGGCGCGAGAGAAGGGTTCGATCCCGATGGACTTCGATGTCCCCTTCGCGCATACACC
GGCCTTCGTTGGCAGTCATGTCACCGGCTATGACAATATGCTGAAGGGCATTCTCGATTATTTCTGGGAAGGCAAGGCCG
GCATGGCGGAAAAGCCCGTGCGCGAGCCAAATGAACGGATCAATTTCATTGGCGGTTTCGATCCCTATACGGTTGGCAAT
CTGCGCGAATTGAAACGCATTCTCGATCTTTGGGATCTTGATTACACGATCCTGTCCGACAATAGCGAAGTGTGGGATAC
ACCGACCGACGGCACGTTCCGCATGTTCGACGGCGGTACCAGCGCCGAGGAAACCACGGCGGCGCTTAATTCCAAGGCGA
CCATCTCACTGCAGGAATATTCCACGCAGAAAACCTTGCCCTACATTGCCGCGCATGGGCAGGAAACAGTCGCGCTGAAT
TGCCCGCTCGGCGTGCAGGCGACCGACAAGCTCCTTCTGGAAATCGCCCGTCTGACCGGCAAGCCAGTTCCGGACGTCAT
GCGCAAGGAGCGTGGCCGTCTTCTCGATGCGATCGCCGATTCCTTCGCCCATATCCATGGTAAGAAATTCGCCATTTATG
GCGACCCCGATCTTTGCCTCGGCATGACGGCTTTCCTGCTGGAGCTCGGGGCGGAGCCGGTGCATGTGCTTTCGACCAAT
GGCGGCAATGAATGGGCCGAAAAGGTCAAAGCTGTCTTCGCGTCTTCGCCTTACGGCGCCAATTGCCATGTCTATCCCAA
TCGCGACCTCTGGCACATGCGTTCCCTGCTCTTCACCGAGCCGGTCGATTTCCTGATTGGCAACACTTACGGCAAATATC
TCGAACGAGACACGGGTACACCTCTGATCCGTGTCGGCTTCCCGATCTTCGACCGCCATCACCATCACCGCTTCCCGATT
TATGGTTATCAGGGCGGCTTGCATCTCCTGCGCTACATGCTCGACGCGATCTTCAATATGATCGACCAGACAACCAATGT
GCCGGCGCATAGCGATTACAGTTTCGATATCATTCGCTGA

Upstream 100 bases:

>100_bases
GCATTCTGCCCGCAGAGGTAGCGCCTGTGTTCGGTCCCTGGCGGCCCGTAGCCTTGCGCCTCCTCTCCTCCTTCCCCCAA
CGACGATCGGATTGCTCTTC

Downstream 100 bases:

>100_bases
TCCATGCGATGACGGCGGCCTTGCAAAAGGCCGCCGCGACGACAGAAAGAAAAATCCTCGCGGCGACCGTCTGGTTTCCG
CGAGGATTTTTTTTTGTAAC

Product: nitrogenase molybdenum-iron protein beta chain

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MPQNAQSVLDHFDLFKQPEYREMFANKKKYFENAVAEVEVDRIREWMKTPEYQEKNFAREALTVNPAKACQPLGAVFCSS
GFMGALSFVHGSQGCVAYYRSHLSRHFKEPCSCVSSSMTEDAAVFGGLTNMIDGLANTYNLYRPAMISVSTTCMAEVIGD
DLNAFIKTAREKGSIPMDFDVPFAHTPAFVGSHVTGYDNMLKGILDYFWEGKAGMAEKPVREPNERINFIGGFDPYTVGN
LRELKRILDLWDLDYTILSDNSEVWDTPTDGTFRMFDGGTSAEETTAALNSKATISLQEYSTQKTLPYIAAHGQETVALN
CPLGVQATDKLLLEIARLTGKPVPDVMRKERGRLLDAIADSFAHIHGKKFAIYGDPDLCLGMTAFLLELGAEPVHVLSTN
GGNEWAEKVKAVFASSPYGANCHVYPNRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRVGFPIFDRHHHHRFPI
YGYQGGLHLLRYMLDAIFNMIDQTTNVPAHSDYSFDIIR

Sequences:

>Translated_519_residues
MPQNAQSVLDHFDLFKQPEYREMFANKKKYFENAVAEVEVDRIREWMKTPEYQEKNFAREALTVNPAKACQPLGAVFCSS
GFMGALSFVHGSQGCVAYYRSHLSRHFKEPCSCVSSSMTEDAAVFGGLTNMIDGLANTYNLYRPAMISVSTTCMAEVIGD
DLNAFIKTAREKGSIPMDFDVPFAHTPAFVGSHVTGYDNMLKGILDYFWEGKAGMAEKPVREPNERINFIGGFDPYTVGN
LRELKRILDLWDLDYTILSDNSEVWDTPTDGTFRMFDGGTSAEETTAALNSKATISLQEYSTQKTLPYIAAHGQETVALN
CPLGVQATDKLLLEIARLTGKPVPDVMRKERGRLLDAIADSFAHIHGKKFAIYGDPDLCLGMTAFLLELGAEPVHVLSTN
GGNEWAEKVKAVFASSPYGANCHVYPNRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRVGFPIFDRHHHHRFPI
YGYQGGLHLLRYMLDAIFNMIDQTTNVPAHSDYSFDIIR
>Mature_518_residues
PQNAQSVLDHFDLFKQPEYREMFANKKKYFENAVAEVEVDRIREWMKTPEYQEKNFAREALTVNPAKACQPLGAVFCSSG
FMGALSFVHGSQGCVAYYRSHLSRHFKEPCSCVSSSMTEDAAVFGGLTNMIDGLANTYNLYRPAMISVSTTCMAEVIGDD
LNAFIKTAREKGSIPMDFDVPFAHTPAFVGSHVTGYDNMLKGILDYFWEGKAGMAEKPVREPNERINFIGGFDPYTVGNL
RELKRILDLWDLDYTILSDNSEVWDTPTDGTFRMFDGGTSAEETTAALNSKATISLQEYSTQKTLPYIAAHGQETVALNC
PLGVQATDKLLLEIARLTGKPVPDVMRKERGRLLDAIADSFAHIHGKKFAIYGDPDLCLGMTAFLLELGAEPVHVLSTNG
GNEWAEKVKAVFASSPYGANCHVYPNRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRVGFPIFDRHHHHRFPIY
GYQGGLHLLRYMLDAIFNMIDQTTNVPAHSDYSFDIIR

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: COG2710

COG function: function code C; Nitrogenase molybdenum-iron protein, alpha and beta chains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 58184; Mature: 58052

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC
>Mature Secondary Structure 
PQNAQSVLDHFDLFKQPEYREMFANKKKYFENAVAEVEVDRIREWMKTPEYQEKNFARE
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
ALTVNPAKACQPLGAVFCSSGFMGALSFVHGSQGCVAYYRSHLSRHFKEPCSCVSSSMTE
HHCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCH
DAAVFGGLTNMIDGLANTYNLYRPAMISVSTTCMAEVIGDDLNAFIKTAREKGSIPMDFD
HHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
VPFAHTPAFVGSHVTGYDNMLKGILDYFWEGKAGMAEKPVREPNERINFIGGFDPYTVGN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCCCCCCCCC
LRELKRILDLWDLDYTILSDNSEVWDTPTDGTFRMFDGGTSAEETTAALNSKATISLQEY
HHHHHHHHHHHCCCEEEECCCCCCCCCCCCCEEEEECCCCCHHHHHHHHCCCEEEEEHHH
STQKTLPYIAAHGQETVALNCPLGVQATDKLLLEIARLTGKPVPDVMRKERGRLLDAIAD
CCCCCCCHHEECCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
SFAHIHGKKFAIYGDPDLCLGMTAFLLELGAEPVHVLSTNGGNEWAEKVKAVFASSPYGA
HHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
NCHVYPNRDLWHMRSLLFTEPVDFLIGNTYGKYLERDTGTPLIRVGFPIFDRHHHHRFPI
CEEECCCCCHHHHHHHHHHCCHHHHHCCHHHHHHHHCCCCCEEEECCCHHCCCCCCCCCE
YGYQGGLHLLRYMLDAIFNMIDQTTNVPAHSDYSFDIIR
EECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11157954; 12597275 [H]