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The map label for this gene is pncA [H]

Identifier: 182678568

GI number: 182678568

Start: 1794655

End: 1795287

Strand: Direct

Name: pncA [H]

Synonym: Bind_1593

Alternate gene names: 182678568

Gene position: 1794655-1795287 (Clockwise)

Preceding gene: 182678567

Following gene: 182678569

Centisome position: 43.04

GC content: 56.08

Gene sequence:

>633_bases
ATGGAAATCGAGACAGGCGATATATTGTTGCTCCTCGACATGCAGAATGATTTTGGTCCGGGTGGTGCTCTCCCGATTTC
CGGTGCCGAAAACATCGCCATGGTTGCAAGTTTGGTGGCGGAATTCTTCGACCATGTCATCGTGACGCAACTTTGGTACC
CACCGGGCCATGAGGCTTTCGTGTCGGCGCATCCCGGCAAAAAGCCGTTCGAGACAGAAGTTTTGTCCTATGGTCCGCAA
ATCCTGTGGCCAGCGCATTGTGTGCAAGGCACGCCCGGAGCCTATCTGCGGCACGATCTTCGTGTGCCGCAAGCCGAATT
GATTCTGCGCCGGGGCCTCGATCATCGGATTGCCGCGAGCTCTGCCTTTGTCGAAAATGATCGTGCGACCAAGACGGGAC
TGGCCGGTTTTCTGCGTGAAAAGGACGTGAAACGTCTGTTTCTGGCGGGCCTGCCTTTTGATTTCACGGTCCGTTTCACT
GCGGAAGATGGCAGACGGGAAGGCTTCGACGTTTTCGTTATCGAGGACGCCTGCTGCGCCCTCGACATCAAGGGCTCAGC
GGCGGAAGCGAAACAGAGTTTGACCAAGCATGGCGTGGCTTGTGTGACAAGCGCCGACATTCTGGAAAGCTGA

Upstream 100 bases:

>100_bases
AAGCCGAAACGGCCATAGGTGAAACCATCATTTTAACGCATGACCCGTGTCTCGAAAGTTTCAGACTTTTGGGAATGACG
TTCTCAAAGGGGAAGGATGC

Downstream 100 bases:

>100_bases
ACCTTTGCTGTGTATGCCACTGACAAGAATGTCACTCCGCCGGAAGAACTGTCATTCCTGTTAATGTGACTCTGCCTTCT
CTTCGGTTCATCGCACAACC

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MEIETGDILLLLDMQNDFGPGGALPISGAENIAMVASLVAEFFDHVIVTQLWYPPGHEAFVSAHPGKKPFETEVLSYGPQ
ILWPAHCVQGTPGAYLRHDLRVPQAELILRRGLDHRIAASSAFVENDRATKTGLAGFLREKDVKRLFLAGLPFDFTVRFT
AEDGRREGFDVFVIEDACCALDIKGSAAEAKQSLTKHGVACVTSADILES

Sequences:

>Translated_210_residues
MEIETGDILLLLDMQNDFGPGGALPISGAENIAMVASLVAEFFDHVIVTQLWYPPGHEAFVSAHPGKKPFETEVLSYGPQ
ILWPAHCVQGTPGAYLRHDLRVPQAELILRRGLDHRIAASSAFVENDRATKTGLAGFLREKDVKRLFLAGLPFDFTVRFT
AEDGRREGFDVFVIEDACCALDIKGSAAEAKQSLTKHGVACVTSADILES
>Mature_210_residues
MEIETGDILLLLDMQNDFGPGGALPISGAENIAMVASLVAEFFDHVIVTQLWYPPGHEAFVSAHPGKKPFETEVLSYGPQ
ILWPAHCVQGTPGAYLRHDLRVPQAELILRRGLDHRIAASSAFVENDRATKTGLAGFLREKDVKRLFLAGLPFDFTVRFT
AEDGRREGFDVFVIEDACCALDIKGSAAEAKQSLTKHGVACVTSADILES

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=207, Percent_Identity=34.2995169082126, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI193206735, Length=220, Percent_Identity=28.1818181818182, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17543130, Length=226, Percent_Identity=28.3185840707965, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71999339, Length=218, Percent_Identity=27.9816513761468, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6321401, Length=212, Percent_Identity=26.8867924528302, Blast_Score=75, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24648247, Length=220, Percent_Identity=28.1818181818182, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22866; Mature: 22866

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIETGDILLLLDMQNDFGPGGALPISGAENIAMVASLVAEFFDHVIVTQLWYPPGHEAF
CCCCCCCEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VSAHPGKKPFETEVLSYGPQILWPAHCVQGTPGAYLRHDLRVPQAELILRRGLDHRIAAS
EECCCCCCCCHHHHHHCCCCEECHHHHHCCCCCHHHHHHCCCCHHHHHHHCCCCCHHHHH
SAFVENDRATKTGLAGFLREKDVKRLFLAGLPFDFTVRFTAEDGRREGFDVFVIEDACCA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCEEE
LDIKGSAAEAKQSLTKHGVACVTSADILES
EECCCCHHHHHHHHHHCCEEEEECHHHHCC
>Mature Secondary Structure
MEIETGDILLLLDMQNDFGPGGALPISGAENIAMVASLVAEFFDHVIVTQLWYPPGHEAF
CCCCCCCEEEEEECCCCCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
VSAHPGKKPFETEVLSYGPQILWPAHCVQGTPGAYLRHDLRVPQAELILRRGLDHRIAAS
EECCCCCCCCHHHHHHCCCCEECHHHHHCCCCCHHHHHHCCCCHHHHHHHCCCCCHHHHH
SAFVENDRATKTGLAGFLREKDVKRLFLAGLPFDFTVRFTAEDGRREGFDVFVIEDACCA
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEEEECCEEE
LDIKGSAAEAKQSLTKHGVACVTSADILES
EECCCCHHHHHHHHHHCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]