Click here to switch to the map view.

The map label for this gene is folE

Identifier: 182678551

GI number: 182678551

Start: 1779032

End: 1779673

Strand: Direct

Name: folE

Synonym: Bind_1576

Alternate gene names: 182678551

Gene position: 1779032-1779673 (Clockwise)

Preceding gene: 182678549

Following gene: 182678554

Centisome position: 42.66

GC content: 56.7

Gene sequence:

>642_bases
ATGGATGACGTGGTTAAGTCCTTACTCCAAAGAACGACTTCTTCCTTGACCAAACCTGCTCCTGCCCGTCCCTCTCGCGA
AGAGGCCGAGGCCGCCGTCGAAGTGCTGTTGCGCTGGACGGGCGATGATCCATCCCGCGAGGGTTTGCGCGATACGCCAA
AGCGCGTCGTCAAGGCTTTCGAGGAATTCTTCTCCGGCTATAACGCCGACGCGTCCGATGTCCTTTCGCGCGTCTTCGAG
GAGGTGCACGGCTACGACAATATGGTGCTCGTGCGGGACATTCCTTTCTCGTCCCACTGCGAGCATCATATGGTGCCCTT
CTTCGGGGTCGCCCATATCGGCTATTATCCTTCGGAAGCCGGTGTGATCGGGCTTTCGAAGCTCGCCCGCCTGGTCGATA
TTTTCGCCAAGAGATTGCAGACTCAAGAGGCTTTGACCGCTCAGATTATTGGGGCGATCGACGAGCATTTGCAGCCCCGC
GGCTGCGCCATTATGCTTGAGGCGGAACATATGTGCATGTCGATGCGCGGAGTGCAAAAACACGGCACATCTACGCTCAC
AACGCAATTTACAGGTGTTTTCAAAAACGATCCGGCCGAGCAGGTTCGCTTCTTCGGCATGGTCCGCAACCCGAAGAGTT
GA

Upstream 100 bases:

>100_bases
TCTAGCAAATATTATCCCAGGTACGCATCTCTAGCCCGTAACGGTGCCTGGGCCAAAAGCAGCAGCGGGCTTTTCCTCTT
AACTTATCGGGAGGCGCCAC

Downstream 100 bases:

>100_bases
CAGCTAGAGCCTCAACCTCGAACATCCGTTTTTGGTTAACGCTCTCTTCGAAAATGTATCAAATCGATCCCACAAATGGG
ATCGATTTCGGTTTGACACT

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MDDVVKSLLQRTTSSLTKPAPARPSREEAEAAVEVLLRWTGDDPSREGLRDTPKRVVKAFEEFFSGYNADASDVLSRVFE
EVHGYDNMVLVRDIPFSSHCEHHMVPFFGVAHIGYYPSEAGVIGLSKLARLVDIFAKRLQTQEALTAQIIGAIDEHLQPR
GCAIMLEAEHMCMSMRGVQKHGTSTLTTQFTGVFKNDPAEQVRFFGMVRNPKS

Sequences:

>Translated_213_residues
MDDVVKSLLQRTTSSLTKPAPARPSREEAEAAVEVLLRWTGDDPSREGLRDTPKRVVKAFEEFFSGYNADASDVLSRVFE
EVHGYDNMVLVRDIPFSSHCEHHMVPFFGVAHIGYYPSEAGVIGLSKLARLVDIFAKRLQTQEALTAQIIGAIDEHLQPR
GCAIMLEAEHMCMSMRGVQKHGTSTLTTQFTGVFKNDPAEQVRFFGMVRNPKS
>Mature_213_residues
MDDVVKSLLQRTTSSLTKPAPARPSREEAEAAVEVLLRWTGDDPSREGLRDTPKRVVKAFEEFFSGYNADASDVLSRVFE
EVHGYDNMVLVRDIPFSSHCEHHMVPFFGVAHIGYYPSEAGVIGLSKLARLVDIFAKRLQTQEALTAQIIGAIDEHLQPR
GCAIMLEAEHMCMSMRGVQKHGTSTLTTQFTGVFKNDPAEQVRFFGMVRNPKS

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=181, Percent_Identity=48.6187845303867, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI4503949, Length=181, Percent_Identity=48.6187845303867, Blast_Score=174, Evalue=7e-44,
Organism=Homo sapiens, GI66932970, Length=139, Percent_Identity=49.6402877697842, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI66932972, Length=139, Percent_Identity=49.6402877697842, Blast_Score=129, Evalue=2e-30,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=35.7954545454545, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17560486, Length=178, Percent_Identity=47.1910112359551, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321706, Length=171, Percent_Identity=50.2923976608187, Blast_Score=168, Evalue=7e-43,
Organism=Drosophila melanogaster, GI24656782, Length=192, Percent_Identity=45.8333333333333, Blast_Score=160, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24656791, Length=192, Percent_Identity=45.8333333333333, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24656796, Length=192, Percent_Identity=45.8333333333333, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_BEII9 (B2IBC4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832697.1
- GeneID:   6200944
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1576
- HOGENOM:   HBG370191
- OMA:   FSSHCEH
- ProtClustDB:   CLSK2405520
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 23769; Mature: 23769

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDVVKSLLQRTTSSLTKPAPARPSREEAEAAVEVLLRWTGDDPSREGLRDTPKRVVKAF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHH
EEFFSGYNADASDVLSRVFEEVHGYDNMVLVRDIPFSSHCEHHMVPFFGVAHIGYYPSEA
HHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCC
GVIGLSKLARLVDIFAKRLQTQEALTAQIIGAIDEHLQPRGCAIMLEAEHMCMSMRGVQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
HGTSTLTTQFTGVFKNDPAEQVRFFGMVRNPKS
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDDVVKSLLQRTTSSLTKPAPARPSREEAEAAVEVLLRWTGDDPSREGLRDTPKRVVKAF
CHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHH
EEFFSGYNADASDVLSRVFEEVHGYDNMVLVRDIPFSSHCEHHMVPFFGVAHIGYYPSEA
HHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCC
GVIGLSKLARLVDIFAKRLQTQEALTAQIIGAIDEHLQPRGCAIMLEAEHMCMSMRGVQK
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
HGTSTLTTQFTGVFKNDPAEQVRFFGMVRNPKS
CCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA