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The map label for this gene is eno

Identifier: 182678446

GI number: 182678446

Start: 1672577

End: 1673860

Strand: Direct

Name: eno

Synonym: Bind_1466

Alternate gene names: 182678446

Gene position: 1672577-1673860 (Clockwise)

Preceding gene: 182678444

Following gene: 182678452

Centisome position: 40.11

GC content: 59.5

Gene sequence:

>1284_bases
ATGACTGCCATCGTCGACATCGCTGCTCGGGAAATCTTGGACAGCCGTGGCAATCCGACCATTGAGGTCGATGTCACCTT
GGAAGACGGCTCGCAGGGACGCGCCGCCGTTCCCTCCGGCGCCTCGACGGGCGCGCATGAGGCCGTCGAATTGCGTGATG
GCGACAAGAGCCGGTTCGGCGGCAAGGGCGTGCTGAAGGCTGTGGAAAATGTCGATCGCGATATTTTCGACGCCTTGAGC
GGCCTCGATGCCGAGGATCAGGTGCATATCGATCAAGTGATGCTCGAACTGGACGGAACGCCGAATAAGGGACGGCTTGG
CGCCAATGCCATTCTCGGTGTCTCGCTAGCCGTTGCCAAGGCGGCGGCCGAGGCATCGAGCCTGCCGCTTTATCGCTATG
TCGGCGGCGTCCAGGCGCGTGTCCTCCCCGTTCCGATGATGAACATCATCAATGGCGGCGCGCATGCCGATAATCCGATC
GATTTCCAGGAATTCATGATCCTGCCGGTCGGCGCGCCGAACCTCAAGGAAGCCGTGCGTTGGGGCGCCGAGGTTTTTCA
CGTGCTGAAAGGCGCGCTGAAAAAGGCTGGCCATAATACCAATGTGGGTGATGAAGGCGGCTTCGCGCCGAATCTGCCCT
CGGCCGAGGCTTCGCTTGAATTCATTGTGAAGGCGATTACCGACGCAGGGTTCAAGCCTGGCGAGGACATCTATCTCGGC
CTCGATTGCGCCTCGACCGAATTCTTCAAGGACGGCAAATATGTCTACGAGGGTGAGGGCAAGACCCGCAACCTTGAGGA
ACAAGCCGCCTATCTTGGCAAGCTGGTCGAGAGCTTCCCGATTGTCACCATCGAAGATGGCATGTCCGAGGATGATTGGG
AGGGCTGGAAGATCCTGACCGATCTGATCGGCAAGAAATGCCAGCTCGTTGGTGACGATCTCTTCGTCACCAATGTCAGC
CGCCTCTCGCAGGGCATTTCGAAGGGCATCGCCAATTCCATCCTGGTCAAGGTCAACCAGATCGGCTCCCTGACCGAAAC
GCTCGCCGCCGTCGATATGGCGCAGCGCGCGGGCTATACGGCGGTCATGTCGCATCGTTCAGGCGAGACAGAGGATTCGA
CCATCGCGGATTTGGCAGTCGCGACCAATTGCGGACAGATCAAGACCGGCTCGCTCGCCCGTTCCGACCGGTTGGCGAAA
TATAACCAACTCATCCGCATCGAGGAAGAACTGGGCAGCCAAGCCGTCTATGCCGGCCGGGCAGCATTGAAGGCTTTGGC
CTGA

Upstream 100 bases:

>100_bases
CCCAAGCATGGATCAGTGGTTCTTACCCGAAACTATATGCCGACCTGCGTCGGATTTTTCACCTTCGCCTGTACCATCTT
TGCTAGTTCAAGGAGTTTCA

Downstream 100 bases:

>100_bases
TCAGGTTGAGGCAAGGTTACGGTTCTATTGTGGAACTGTGATCCGGCGTTAGAAAAAGAAACGGCGGTTTCGCGAGCGCG
GAGCCGCCATTTTCTTTTGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTAIVDIAAREILDSRGNPTIEVDVTLEDGSQGRAAVPSGASTGAHEAVELRDGDKSRFGGKGVLKAVENVDRDIFDALS
GLDAEDQVHIDQVMLELDGTPNKGRLGANAILGVSLAVAKAAAEASSLPLYRYVGGVQARVLPVPMMNIINGGAHADNPI
DFQEFMILPVGAPNLKEAVRWGAEVFHVLKGALKKAGHNTNVGDEGGFAPNLPSAEASLEFIVKAITDAGFKPGEDIYLG
LDCASTEFFKDGKYVYEGEGKTRNLEEQAAYLGKLVESFPIVTIEDGMSEDDWEGWKILTDLIGKKCQLVGDDLFVTNVS
RLSQGISKGIANSILVKVNQIGSLTETLAAVDMAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGSQAVYAGRAALKALA

Sequences:

>Translated_427_residues
MTAIVDIAAREILDSRGNPTIEVDVTLEDGSQGRAAVPSGASTGAHEAVELRDGDKSRFGGKGVLKAVENVDRDIFDALS
GLDAEDQVHIDQVMLELDGTPNKGRLGANAILGVSLAVAKAAAEASSLPLYRYVGGVQARVLPVPMMNIINGGAHADNPI
DFQEFMILPVGAPNLKEAVRWGAEVFHVLKGALKKAGHNTNVGDEGGFAPNLPSAEASLEFIVKAITDAGFKPGEDIYLG
LDCASTEFFKDGKYVYEGEGKTRNLEEQAAYLGKLVESFPIVTIEDGMSEDDWEGWKILTDLIGKKCQLVGDDLFVTNVS
RLSQGISKGIANSILVKVNQIGSLTETLAAVDMAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAK
YNQLIRIEEELGSQAVYAGRAALKALA
>Mature_426_residues
TAIVDIAAREILDSRGNPTIEVDVTLEDGSQGRAAVPSGASTGAHEAVELRDGDKSRFGGKGVLKAVENVDRDIFDALSG
LDAEDQVHIDQVMLELDGTPNKGRLGANAILGVSLAVAKAAAEASSLPLYRYVGGVQARVLPVPMMNIINGGAHADNPID
FQEFMILPVGAPNLKEAVRWGAEVFHVLKGALKKAGHNTNVGDEGGFAPNLPSAEASLEFIVKAITDAGFKPGEDIYLGL
DCASTEFFKDGKYVYEGEGKTRNLEEQAAYLGKLVESFPIVTIEDGMSEDDWEGWKILTDLIGKKCQLVGDDLFVTNVSR
LSQGISKGIANSILVKVNQIGSLTETLAAVDMAQRAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKY
NQLIRIEEELGSQAVYAGRAALKALA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=53.2558139534884, Blast_Score=419, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=432, Percent_Identity=53.0092592592593, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=432, Percent_Identity=53.0092592592593, Blast_Score=418, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=437, Percent_Identity=52.1739130434783, Blast_Score=415, Evalue=1e-116,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=48.1395348837209, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=25.6716417910448, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=25.6716417910448, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=25.6716417910448, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789141, Length=432, Percent_Identity=62.2685185185185, Blast_Score=510, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI71995829, Length=430, Percent_Identity=52.5581395348837, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=430, Percent_Identity=52.5581395348837, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=47.8947368421053, Blast_Score=176, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.1916859122402, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=50.6944444444444, Blast_Score=389, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=48.9607390300231, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=48.9607390300231, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50.6944444444444, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=431, Percent_Identity=50.8120649651972, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=431, Percent_Identity=50.8120649651972, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=431, Percent_Identity=50.8120649651972, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=431, Percent_Identity=50.8120649651972, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=50.8120649651972, Blast_Score=384, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=50.8120649651972, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_BEII9 (B2IKR4)

Other databases:

- EMBL:   CP001016
- RefSeq:   YP_001832592.1
- ProteinModelPortal:   B2IKR4
- GeneID:   6200853
- GenomeReviews:   CP001016_GR
- KEGG:   bid:Bind_1466
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45277; Mature: 45145

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVDIAAREILDSRGNPTIEVDVTLEDGSQGRAAVPSGASTGAHEAVELRDGDKSRFG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVENVDRDIFDALSGLDAEDQVHIDQVMLELDGTPNKGRLGANAILGVSLAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEASSLPLYRYVGGVQARVLPVPMMNIINGGAHADNPIDFQEFMILPVGAPNLKEAVR
HHHHHHCCCHHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
WGAEVFHVLKGALKKAGHNTNVGDEGGFAPNLPSAEASLEFIVKAITDAGFKPGEDIYLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCASTEFFKDGKYVYEGEGKTRNLEEQAAYLGKLVESFPIVTIEDGMSEDDWEGWKILT
EECCCCHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
DLIGKKCQLVGDDLFVTNVSRLSQGISKGIANSILVKVNQIGSLTETLAAVDMAQRAGYT
HHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCC
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGSQAVYAGR
EEHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AALKALA
HHHHHCC
>Mature Secondary Structure 
TAIVDIAAREILDSRGNPTIEVDVTLEDGSQGRAAVPSGASTGAHEAVELRDGDKSRFG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCEECCCCCCCCCCCEEEECCCCCCCCC
GKGVLKAVENVDRDIFDALSGLDAEDQVHIDQVMLELDGTPNKGRLGANAILGVSLAVAK
CHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHH
AAAEASSLPLYRYVGGVQARVLPVPMMNIINGGAHADNPIDFQEFMILPVGAPNLKEAVR
HHHHHHCCCHHHHHCCCCEEEECCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHH
WGAEVFHVLKGALKKAGHNTNVGDEGGFAPNLPSAEASLEFIVKAITDAGFKPGEDIYLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
LDCASTEFFKDGKYVYEGEGKTRNLEEQAAYLGKLVESFPIVTIEDGMSEDDWEGWKILT
EECCCCHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHH
DLIGKKCQLVGDDLFVTNVSRLSQGISKGIANSILVKVNQIGSLTETLAAVDMAQRAGYT
HHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHCCCC
AVMSHRSGETEDSTIADLAVATNCGQIKTGSLARSDRLAKYNQLIRIEEELGSQAVYAGR
EEHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
AALKALA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA