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The map label for this gene is purD [H]

Identifier: 182678366

GI number: 182678366

Start: 1587807

End: 1588985

Strand: Direct

Name: purD [H]

Synonym: Bind_1383

Alternate gene names: 182678366

Gene position: 1587807-1588985 (Clockwise)

Preceding gene: 182678365

Following gene: 182678370

Centisome position: 38.08

GC content: 56.32

Gene sequence:

>1179_bases
ATGCGCGTTTTGGTAATCGGGGATGGCGCGAGCGAACATGCCTTGTGCTGGAAGCTTGTGGAATCCCCCCTCGTTGACGA
ACTGGCCTGCGCTCCAGGCGGCGCCGGAGTGGCCAATCTCGCCGAATGCGTGCCGATTTCCATGACCGCGCGCGATACGA
TTCTCACCCATTGCGACGATAATGAAGTGGAATTCGTCATTGTCGATTCGATCGCGTCCATGGAGATCGCGCTCGTCGAT
ATGTTGAATCGCAACGGCTATCCCTCGTTCGGCCCTGATATGGGCGGCGTCAAACTGGAGTCTTCGAAGCTCTTCGCCAA
GGAATTTTGCAAACGCAACACGATTCCGACCGCGCATTGGGAGGCTTTCACCGATGCGGCTTCGGCCAAAGCTTATGTCG
CGGGTGCTACCTTGCCACTCGTCGTCAAGAGTGACCTTCGCGTCGATGGCGCCAAGGTGGTGATTTGCCAAACCAAGGAA
GATGCCGAGGCGGCGATCGATGCCCGTTTTACAGGGGCAGAAAATCAAAACGTCATCATTGAGGAATTCCTGACCGGCCA
GGAAATCACTTATTCGGCCATCACCGACGGCAATGTCGTCTTGCCCTTGACCACGACGACGCCAGCCTGGAACGGCGATA
ATACCCCCGCCGATCTGCCAGGCTCTCTCTCGCCAGCGCCTGGCGTGACGCCTGAATTAGAGAGCGAATTGGTCGATCTT
TTTTTGTTGCCGACCGTCCAGCAGATGAAGGAAGAGCGCCACCAGTTTCGTGGTCATCTGCAAGTCAATCTCAAGCTGAC
CGCCGAAGGGCCGAAGCTTATGGATTACAAGGTCCATTTTTCAGACCCGGAATGGCAGGTGATCATGCTGCGCATGAAGG
GTGATCTGATGCCGGCGCTGATCTCGTCTTTTGACGAGATGCTGTATCGTTTCAACCCGTTCCGCTGGCATGAGGAATCG
GCCATGGCGGTTGTCGTGAAGACGGATGGGAAGCGGGATCCGGAGCAGGTTTGCGCGGCCGTTGATGCGGCCGAGGAACC
GGACGGCGATATTGTCGTATTCCAATCCTATCAGGACAGGGAATTAGGCATTACCGCATCCGGCAAGGATCTCGATGATG
TGCGCAAGCGGCTCTTGGCAGCCACCGAGCGCCTGAAAAATGTGATGAGCTTCGAATAG

Upstream 100 bases:

>100_bases
CGAGAGCGGATGCTTTAGTGAGGGACGAGTAGGGCCCGCCGGCAAGCTTTGTTTCCGGCAGCGGCGCAGGATTGAATTCG
GTTGGCGGGTGAGGAACTAA

Downstream 100 bases:

>100_bases
AGACAGGTTCATTAAAGAAGAAAGCGGCCTTTACGGCCGCTTTTTTAGTTTTTACCGAAAGATGTTGTTGTCCGGCTTGA
GAACCGCCATTGGCGGCTTT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MRVLVIGDGASEHALCWKLVESPLVDELACAPGGAGVANLAECVPISMTARDTILTHCDDNEVEFVIVDSIASMEIALVD
MLNRNGYPSFGPDMGGVKLESSKLFAKEFCKRNTIPTAHWEAFTDAASAKAYVAGATLPLVVKSDLRVDGAKVVICQTKE
DAEAAIDARFTGAENQNVIIEEFLTGQEITYSAITDGNVVLPLTTTTPAWNGDNTPADLPGSLSPAPGVTPELESELVDL
FLLPTVQQMKEERHQFRGHLQVNLKLTAEGPKLMDYKVHFSDPEWQVIMLRMKGDLMPALISSFDEMLYRFNPFRWHEES
AMAVVVKTDGKRDPEQVCAAVDAAEEPDGDIVVFQSYQDRELGITASGKDLDDVRKRLLAATERLKNVMSFE

Sequences:

>Translated_392_residues
MRVLVIGDGASEHALCWKLVESPLVDELACAPGGAGVANLAECVPISMTARDTILTHCDDNEVEFVIVDSIASMEIALVD
MLNRNGYPSFGPDMGGVKLESSKLFAKEFCKRNTIPTAHWEAFTDAASAKAYVAGATLPLVVKSDLRVDGAKVVICQTKE
DAEAAIDARFTGAENQNVIIEEFLTGQEITYSAITDGNVVLPLTTTTPAWNGDNTPADLPGSLSPAPGVTPELESELVDL
FLLPTVQQMKEERHQFRGHLQVNLKLTAEGPKLMDYKVHFSDPEWQVIMLRMKGDLMPALISSFDEMLYRFNPFRWHEES
AMAVVVKTDGKRDPEQVCAAVDAAEEPDGDIVVFQSYQDRELGITASGKDLDDVRKRLLAATERLKNVMSFE
>Mature_392_residues
MRVLVIGDGASEHALCWKLVESPLVDELACAPGGAGVANLAECVPISMTARDTILTHCDDNEVEFVIVDSIASMEIALVD
MLNRNGYPSFGPDMGGVKLESSKLFAKEFCKRNTIPTAHWEAFTDAASAKAYVAGATLPLVVKSDLRVDGAKVVICQTKE
DAEAAIDARFTGAENQNVIIEEFLTGQEITYSAITDGNVVLPLTTTTPAWNGDNTPADLPGSLSPAPGVTPELESELVDL
FLLPTVQQMKEERHQFRGHLQVNLKLTAEGPKLMDYKVHFSDPEWQVIMLRMKGDLMPALISSFDEMLYRFNPFRWHEES
AMAVVVKTDGKRDPEQVCAAVDAAEEPDGDIVVFQSYQDRELGITASGKDLDDVRKRLLAATERLKNVMSFE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=410, Percent_Identity=32.9268292682927, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI4503915, Length=410, Percent_Identity=32.9268292682927, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI209869995, Length=410, Percent_Identity=32.9268292682927, Blast_Score=195, Evalue=6e-50,
Organism=Homo sapiens, GI209869993, Length=410, Percent_Identity=32.9268292682927, Blast_Score=195, Evalue=6e-50,
Organism=Escherichia coli, GI1790438, Length=343, Percent_Identity=34.9854227405248, Blast_Score=194, Evalue=6e-51,
Organism=Caenorhabditis elegans, GI17567511, Length=375, Percent_Identity=27.4666666666667, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6321203, Length=356, Percent_Identity=29.2134831460674, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI62471619, Length=346, Percent_Identity=33.8150289017341, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24582400, Length=346, Percent_Identity=33.8150289017341, Blast_Score=155, Evalue=5e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 42921; Mature: 42921

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVIGDGASEHALCWKLVESPLVDELACAPGGAGVANLAECVPISMTARDTILTHCDD
CEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCEEEEECCCEEEEECCC
NEVEFVIVDSIASMEIALVDMLNRNGYPSFGPDMGGVKLESSKLFAKEFCKRNTIPTAHW
CCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCHH
EAFTDAASAKAYVAGATLPLVVKSDLRVDGAKVVICQTKEDAEAAIDARFTGAENQNVII
HHHHHHCCCCEEEECCEEEEEEECCCEECCEEEEEEECCCCCHHHEEEEECCCCCCCEEE
EEFLTGQEITYSAITDGNVVLPLTTTTPAWNGDNTPADLPGSLSPAPGVTPELESELVDL
EHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FLLPTVQQMKEERHQFRGHLQVNLKLTAEGPKLMDYKVHFSDPEWQVIMLRMKGDLMPAL
HHCCCHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEEEECCCCEEEEEEEECCCHHHHH
ISSFDEMLYRFNPFRWHEESAMAVVVKTDGKRDPEQVCAAVDAAEEPDGDIVVFQSYQDR
HHHHHHHHHHCCCCEEECCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCC
ELGITASGKDLDDVRKRLLAATERLKNVMSFE
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRVLVIGDGASEHALCWKLVESPLVDELACAPGGAGVANLAECVPISMTARDTILTHCDD
CEEEEEECCCCCHHHHHHHHHCCCHHHHHCCCCCCCHHHHHHHCEEEEECCCEEEEECCC
NEVEFVIVDSIASMEIALVDMLNRNGYPSFGPDMGGVKLESSKLFAKEFCKRNTIPTAHW
CCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCHH
EAFTDAASAKAYVAGATLPLVVKSDLRVDGAKVVICQTKEDAEAAIDARFTGAENQNVII
HHHHHHCCCCEEEECCEEEEEEECCCEECCEEEEEEECCCCCHHHEEEEECCCCCCCEEE
EEFLTGQEITYSAITDGNVVLPLTTTTPAWNGDNTPADLPGSLSPAPGVTPELESELVDL
EHHCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
FLLPTVQQMKEERHQFRGHLQVNLKLTAEGPKLMDYKVHFSDPEWQVIMLRMKGDLMPAL
HHCCCHHHHHHHHHHHCCEEEEEEEEEECCCEEEEEEEEECCCCEEEEEEEECCCHHHHH
ISSFDEMLYRFNPFRWHEESAMAVVVKTDGKRDPEQVCAAVDAAEEPDGDIVVFQSYQDR
HHHHHHHHHHCCCCEEECCCEEEEEEECCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCC
ELGITASGKDLDDVRKRLLAATERLKNVMSFE
EEEEEECCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968 [H]