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The map label for this gene is btuE [H]

Identifier: 182678298

GI number: 182678298

Start: 1521809

End: 1522330

Strand: Direct

Name: btuE [H]

Synonym: Bind_1315

Alternate gene names: 182678298

Gene position: 1521809-1522330 (Clockwise)

Preceding gene: 182678297

Following gene: 182678299

Centisome position: 36.49

GC content: 55.36

Gene sequence:

>522_bases
ATGAGCAAGCTTTACGATATTACCGTAAAAACCATTGAGGGACCGAGCGAGACGATCGGCGAATACAAAGGCAATGTCAT
ATTGATCGTCAATACGGCATCGGCCTGCGGTCTCACGCCGCAATATCTTGCCCTGGAAAAGATCTATGAGACCTATATGG
ACCGGGGCTTTGTGGTGCTCGGGTTTCCCTGCAATGATTTCGGTGCCCAGGAGCCGGGGACCGAATTGGAAATTCAGCGT
TTTTGTGAGGCGAAATTCAACGTCCAGTTTCCCATGTTCGCCAAGATCAGCGTCAAGCCAGGGTCTCGCCATCCACTCTA
TGAGGAATTGATCGCGCGCCAGCCCAAGGCCCGCACCAATCCGGGCGCAGCGCCGAAGGAAGGCAGCGACATTTCCTGGA
ATTTCGAAAAATTCCTGGTCAGTCGCGACGGCGAGGTCATTGACCGTTTCGCTCCGCAGGTGACACCTGATTCTCCTCTG
GTCATCGAGGCCGTCGAGAAGGCGCTCGGGCCGACGGCCTGA

Upstream 100 bases:

>100_bases
GCGGGACGAAGAAGCCCTCGAACGCCGAACGAGACGTTGGCGAGACATGGAAGCGGCTGCCCGCCTTTTCCAGTCCGTCC
AATCATGAGGATAGGCAATC

Downstream 100 bases:

>100_bases
GATTGTTCTTCCATGGCAAAAGTCCCCATGGCAAAAGTCCCCATGGCAAAAGTCCATGGTCTTGAGATCAGGCGTGCCGA
GGCCGGCGATTTTGAAGGCC

Product: glutathione peroxidase

Products: ADP; phosphate; cob(I)alamin [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 173; Mature: 172

Protein sequence:

>173_residues
MSKLYDITVKTIEGPSETIGEYKGNVILIVNTASACGLTPQYLALEKIYETYMDRGFVVLGFPCNDFGAQEPGTELEIQR
FCEAKFNVQFPMFAKISVKPGSRHPLYEELIARQPKARTNPGAAPKEGSDISWNFEKFLVSRDGEVIDRFAPQVTPDSPL
VIEAVEKALGPTA

Sequences:

>Translated_173_residues
MSKLYDITVKTIEGPSETIGEYKGNVILIVNTASACGLTPQYLALEKIYETYMDRGFVVLGFPCNDFGAQEPGTELEIQR
FCEAKFNVQFPMFAKISVKPGSRHPLYEELIARQPKARTNPGAAPKEGSDISWNFEKFLVSRDGEVIDRFAPQVTPDSPL
VIEAVEKALGPTA
>Mature_172_residues
SKLYDITVKTIEGPSETIGEYKGNVILIVNTASACGLTPQYLALEKIYETYMDRGFVVLGFPCNDFGAQEPGTELEIQRF
CEAKFNVQFPMFAKISVKPGSRHPLYEELIARQPKARTNPGAAPKEGSDISWNFEKFLVSRDGEVIDRFAPQVTPDSPLV
IEAVEKALGPTA

Specific function: Not essential for B12 transport; however, it is an auxiliary component of the transport system [H]

COG id: COG0386

COG function: function code O; Glutathione peroxidase

Gene ontology:

Cell location: Periplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutathione peroxidase family [H]

Homologues:

Organism=Homo sapiens, GI75709200, Length=161, Percent_Identity=38.5093167701863, Blast_Score=111, Evalue=3e-25,
Organism=Homo sapiens, GI90903240, Length=161, Percent_Identity=38.5093167701863, Blast_Score=111, Evalue=3e-25,
Organism=Homo sapiens, GI15618997, Length=153, Percent_Identity=37.9084967320261, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI90903238, Length=136, Percent_Identity=38.2352941176471, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI192455698, Length=171, Percent_Identity=30.9941520467836, Blast_Score=92, Evalue=2e-19,
Organism=Homo sapiens, GI6006001, Length=164, Percent_Identity=35.9756097560976, Blast_Score=83, Evalue=1e-16,
Organism=Homo sapiens, GI4557629, Length=152, Percent_Identity=36.8421052631579, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI41406084, Length=150, Percent_Identity=34, Blast_Score=77, Evalue=6e-15,
Organism=Homo sapiens, GI32967607, Length=163, Percent_Identity=28.8343558282209, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI33186887, Length=153, Percent_Identity=33.9869281045752, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1788003, Length=173, Percent_Identity=51.4450867052023, Blast_Score=182, Evalue=9e-48,
Organism=Caenorhabditis elegans, GI17506887, Length=171, Percent_Identity=39.1812865497076, Blast_Score=123, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI17535473, Length=170, Percent_Identity=41.1764705882353, Blast_Score=118, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI193210707, Length=160, Percent_Identity=36.875, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI193209493, Length=183, Percent_Identity=31.1475409836066, Blast_Score=105, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI71993584, Length=154, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17550320, Length=183, Percent_Identity=31.1475409836066, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71993573, Length=94, Percent_Identity=35.1063829787234, Blast_Score=66, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71999642, Length=75, Percent_Identity=36, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319721, Length=157, Percent_Identity=42.6751592356688, Blast_Score=140, Evalue=7e-35,
Organism=Saccharomyces cerevisiae, GI6322228, Length=169, Percent_Identity=40.8284023668639, Blast_Score=135, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6322826, Length=156, Percent_Identity=36.5384615384615, Blast_Score=109, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24656777, Length=171, Percent_Identity=38.5964912280702, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24656767, Length=171, Percent_Identity=38.5964912280702, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24656772, Length=171, Percent_Identity=38.5964912280702, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI85726443, Length=171, Percent_Identity=35.672514619883, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000889
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00255 GSHPx [H]

EC number: NA

Molecular weight: Translated: 19120; Mature: 18989

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00460 GLUTATHIONE_PEROXID_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLYDITVKTIEGPSETIGEYKGNVILIVNTASACGLTPQYLALEKIYETYMDRGFVVL
CCCEEEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEE
GFPCNDFGAQEPGTELEIQRFCEAKFNVQFPMFAKISVKPGSRHPLYEELIARQPKARTN
EECCCCCCCCCCCCHHHHHHHHCHHCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCC
PGAAPKEGSDISWNFEKFLVSRDGEVIDRFAPQVTPDSPLVIEAVEKALGPTA
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKLYDITVKTIEGPSETIGEYKGNVILIVNTASACGLTPQYLALEKIYETYMDRGFVVL
CCEEEEEEEEECCCHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCEEEE
GFPCNDFGAQEPGTELEIQRFCEAKFNVQFPMFAKISVKPGSRHPLYEELIARQPKARTN
EECCCCCCCCCCCCHHHHHHHHCHHCCCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCC
PGAAPKEGSDISWNFEKFLVSRDGEVIDRFAPQVTPDSPLVIEAVEKALGPTA
CCCCCCCCCCCCCCHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; cob(I)alamin [Periplasm]; H2O [C]

Specific reaction: ATP + cob(I)alamin [Periplasm] + H2O = ADP + phosphate + cob(I)alamin [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3528129; 9097039; 9278503 [H]