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The map label for this gene is ppaX [H]

Identifier: 182678296

GI number: 182678296

Start: 1520241

End: 1520939

Strand: Direct

Name: ppaX [H]

Synonym: Bind_1313

Alternate gene names: 182678296

Gene position: 1520241-1520939 (Clockwise)

Preceding gene: 182678295

Following gene: 182678297

Centisome position: 36.46

GC content: 59.8

Gene sequence:

>699_bases
ATGCTCCTGAATGACACGGTGTCCTTACGCGAAACTGGCCAGAACCGAAGATTAGTCATCTTTGACGTCGATGGCACATT
GGTCGATAGCCAGGATTTCATCGTCGAGGCGATGCGGCGGGCCTTCGCCGTCCATGACCTGCCCATACCCGAGCGCAAAC
AGGCGCTTTCAATCGTCGGTCTTTCTTTGCATGAGGCTTTCACCGTTCTGGTTGGGCCGGATGCGCCGATCGCCGGGCTG
GTCGAGACCTATAAGGAGGCCTGGAACGCCATGCGGGCCGATCCCGCCTATGACGATCCTTTCTATCCTGGTGCACGAGA
GACTTTGGATGCTTTTGCGGCGCGCACCGATCTCGTGCTCGGCATCGCCACCGGCAAGTCCCGGCGCGGCGTGAAGCATC
TGCTCGATCGCTGGAATTTGCACAGCCATTTCGAAACGGTTCAGACCTCGGATGATCATCCCTCGAAACCGGCGCCCGAC
ATGGTCCTTGCGGCCCTTGCGGAAACCGGTGTCGAGCCCGCTGATGCCTTCATGATCGGCGACACGGCTTATGATATGGA
AATGGCTTGTGCTGCAGGCGTGCGACCGATTGGGGTCGCCTGGGGCTATCATGAGAGGGCCGTGCTCGAGGCCGCTGGCG
CTGAACGGGTGGTCGCTGATTTTGCCGAAATCGAGGTCTTGTTGGGCCCAACCGGCTGA

Upstream 100 bases:

>100_bases
CGCGGCGGCATTTTGGATGTCACGGCGCCTCTGCCTCCGCATATGCAAAAAACCTTCGATCTCTTCGGATTCGACATAAA
GCACGAGGACCCGATCGATC

Downstream 100 bases:

>100_bases
AAGACCGACCCAGCATCGCGAACAGGAAAAGGACGATGGCTGAACATCGCAACCCAGACGATCTTGCCGCTCTGTTTCCG
CAAGCGGCTAGCGAGCCGAT

Product: HAD family hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 232; Mature: 232

Protein sequence:

>232_residues
MLLNDTVSLRETGQNRRLVIFDVDGTLVDSQDFIVEAMRRAFAVHDLPIPERKQALSIVGLSLHEAFTVLVGPDAPIAGL
VETYKEAWNAMRADPAYDDPFYPGARETLDAFAARTDLVLGIATGKSRRGVKHLLDRWNLHSHFETVQTSDDHPSKPAPD
MVLAALAETGVEPADAFMIGDTAYDMEMACAAGVRPIGVAWGYHERAVLEAAGAERVVADFAEIEVLLGPTG

Sequences:

>Translated_232_residues
MLLNDTVSLRETGQNRRLVIFDVDGTLVDSQDFIVEAMRRAFAVHDLPIPERKQALSIVGLSLHEAFTVLVGPDAPIAGL
VETYKEAWNAMRADPAYDDPFYPGARETLDAFAARTDLVLGIATGKSRRGVKHLLDRWNLHSHFETVQTSDDHPSKPAPD
MVLAALAETGVEPADAFMIGDTAYDMEMACAAGVRPIGVAWGYHERAVLEAAGAERVVADFAEIEVLLGPTG
>Mature_232_residues
MLLNDTVSLRETGQNRRLVIFDVDGTLVDSQDFIVEAMRRAFAVHDLPIPERKQALSIVGLSLHEAFTVLVGPDAPIAGL
VETYKEAWNAMRADPAYDDPFYPGARETLDAFAARTDLVLGIATGKSRRGVKHLLDRWNLHSHFETVQTSDDHPSKPAPD
MVLAALAETGVEPADAFMIGDTAYDMEMACAAGVRPIGVAWGYHERAVLEAAGAERVVADFAEIEVLLGPTG

Specific function: Hydrolyzes pyrophosphate formed during P-Ser-HPr dephosphorylation by HPrK/P. Might play a role in controlling the intracellular pyrophosphate pool [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. PpaX family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=212, Percent_Identity=27.3584905660377, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.6.1.1 [H]

Molecular weight: Translated: 25198; Mature: 25198

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNDTVSLRETGQNRRLVIFDVDGTLVDSQDFIVEAMRRAFAVHDLPIPERKQALSIVG
CCCCCCCHHHHCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LSLHEAFTVLVGPDAPIAGLVETYKEAWNAMRADPAYDDPFYPGARETLDAFAARTDLVL
HHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHEEE
GIATGKSRRGVKHLLDRWNLHSHFETVQTSDDHPSKPAPDMVLAALAETGVEPADAFMIG
EEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEC
DTAYDMEMACAAGVRPIGVAWGYHERAVLEAAGAERVVADFAEIEVLLGPTG
CCHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHEEEEECCCC
>Mature Secondary Structure
MLLNDTVSLRETGQNRRLVIFDVDGTLVDSQDFIVEAMRRAFAVHDLPIPERKQALSIVG
CCCCCCCHHHHCCCCCEEEEEECCCCEECCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
LSLHEAFTVLVGPDAPIAGLVETYKEAWNAMRADPAYDDPFYPGARETLDAFAARTDLVL
HHHHHEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHEEE
GIATGKSRRGVKHLLDRWNLHSHFETVQTSDDHPSKPAPDMVLAALAETGVEPADAFMIG
EEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEC
DTAYDMEMACAAGVRPIGVAWGYHERAVLEAAGAERVVADFAEIEVLLGPTG
CCHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377; 9465101; 12359880 [H]