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The map label for this gene is hoxA [H]

Identifier: 182678146

GI number: 182678146

Start: 1342683

End: 1344173

Strand: Direct

Name: hoxA [H]

Synonym: Bind_1161

Alternate gene names: 182678146

Gene position: 1342683-1344173 (Clockwise)

Preceding gene: 182678145

Following gene: 182678147

Centisome position: 32.2

GC content: 59.36

Gene sequence:

>1491_bases
ATGAACGATCTTCCACCCATATTGGTCGTGGACGATGAACCACGCTCGGTGGAAGTGATGGCCCGTGTCCTGCGCGAGGA
ATTCGACGTGCATGTCGCGAACGGCGCGGAAGCGGCGCTCGCGGTCCTGAATGCGCATTGGATTCAGGCGATCTTTTGCG
ATCAGCGCATGCCCGGCATGACAGGCGTGAAGCTTCTGGCGGATGTTCGCCAGCGCTGGCCCGATATCGTGCGGATTATC
GTTACCGGCTACACCGACCCGGACGATATGATCGAAGCCATCAACGAGGCTGGCATTTATCAATATATCACCAAGCCCTG
GCATCCGGATCAGCTTCTGCTCGCCGCGCGCAATGCCACGCAGCTTTTCGCGATGCAGCGCGAGCATGATCGTTTGTCGC
TCGAATTGCGTCTTGCCCGGCCGGCTGTGGAAAAACGCCTGGTGCGTGAACGGGCGCTCGTGCGCGAAACCTATCATTTC
GAAGCTCTCGTTCGCACGCAGGGCAGCCCCCTGAACGAGACTTGTCACCAAGCGGCGAAAGCGGCCACGTTCGATATTCC
TGTGCTAATCCAGGGTGAGACAGGAACCGGCAAGGAACTGCTGGCCCGCGCCATTCATTATGCGAGTCAAAGGGCTAGCG
GGCCATTTTTGGCCGTCAATTGCGGGGCGATTCCGGATACGCTTCTCGAATCTGAATTGTTTGGCCATAAAAAGGGTTCG
TTCACGGGGGCTCATGCCAATCGGCGCGGCCTGCTCGAGGAAGCGGATGGTGGCACTTTGCTGCTCGACGAGATCGGCGA
TATTTCTCCGGCCTTTCAAGTCAAGCTCTTGCGTTTTCTGCAAGAGGGAGAAATCCGCGCGGTCGGTGCCAATGAATCGC
GCCGCGCCGATGTCCGTATCCTTTCGGCCACGCATCGCGACCTGCGCGAGGAAGTGAGGGCCGGACGTTTCCGCGAAGAT
CTCTATTTTCGCCTCGCCGCTATGGCGCTGACCATGCCGCCCTTGCGTAAACGGCCGGGGGATGTACGCGTCTTGGCTGA
TCATCTTTTGGATACATTGTCGGCGCGCCACGGACGGCGTGTCCATGGGTTTACAGCGGAAGCCATGACCTGCCTGATGG
ATTATGACTGGCCCGGCAATGTGCGGGAATTGCAGAATGAAGTTTTGCGGATGCTGGTGCTGACAGAGGGCGATTTACTC
GGCGCTGATCTCCTGTCGCCTGGCGTTCTACATCGTGTGGCGGGGGCACGCGCGAGTGGTCATGCCGATGGGGAAGCCAT
GGAAAGCCCGACGCTTTCCACACCCGAGAGCGGCCCCTTGCAGGAGAGGATCGAGCGGATCGAAGCTCAGATCCTGATGG
AGACGATCGTGCGTTGCCGTTGGAACAAGAGCCGCGCGGCCAAGGAACTCGGCCTTTCCCGCGTTGGTTTGCGGGCCAAG
CTCGAACGCTATGGCATAGCACCGGACGCAGGTCGCGAGACGATACAATAG

Upstream 100 bases:

>100_bases
GCTTGGCTGGTTGCGGGCGGAGCGACAGGCTTTTCTCGCGGAATTGGCTGGTGAGGCCGAGGCACGCGCAGCGCTGCTAC
GGGCGGCGGAATAGGTTCTG

Downstream 100 bases:

>100_bases
AGGGCGGACCAATGTGTTTGGGAATTCCGGGACGGATCTCTGCCATTCTTGACGAGGCGAACAGCCTGGTTGAGGTGGAT
GTTGCAGGTGTCAAGCGGAG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MNDLPPILVVDDEPRSVEVMARVLREEFDVHVANGAEAALAVLNAHWIQAIFCDQRMPGMTGVKLLADVRQRWPDIVRII
VTGYTDPDDMIEAINEAGIYQYITKPWHPDQLLLAARNATQLFAMQREHDRLSLELRLARPAVEKRLVRERALVRETYHF
EALVRTQGSPLNETCHQAAKAATFDIPVLIQGETGTGKELLARAIHYASQRASGPFLAVNCGAIPDTLLESELFGHKKGS
FTGAHANRRGLLEEADGGTLLLDEIGDISPAFQVKLLRFLQEGEIRAVGANESRRADVRILSATHRDLREEVRAGRFRED
LYFRLAAMALTMPPLRKRPGDVRVLADHLLDTLSARHGRRVHGFTAEAMTCLMDYDWPGNVRELQNEVLRMLVLTEGDLL
GADLLSPGVLHRVAGARASGHADGEAMESPTLSTPESGPLQERIERIEAQILMETIVRCRWNKSRAAKELGLSRVGLRAK
LERYGIAPDAGRETIQ

Sequences:

>Translated_496_residues
MNDLPPILVVDDEPRSVEVMARVLREEFDVHVANGAEAALAVLNAHWIQAIFCDQRMPGMTGVKLLADVRQRWPDIVRII
VTGYTDPDDMIEAINEAGIYQYITKPWHPDQLLLAARNATQLFAMQREHDRLSLELRLARPAVEKRLVRERALVRETYHF
EALVRTQGSPLNETCHQAAKAATFDIPVLIQGETGTGKELLARAIHYASQRASGPFLAVNCGAIPDTLLESELFGHKKGS
FTGAHANRRGLLEEADGGTLLLDEIGDISPAFQVKLLRFLQEGEIRAVGANESRRADVRILSATHRDLREEVRAGRFRED
LYFRLAAMALTMPPLRKRPGDVRVLADHLLDTLSARHGRRVHGFTAEAMTCLMDYDWPGNVRELQNEVLRMLVLTEGDLL
GADLLSPGVLHRVAGARASGHADGEAMESPTLSTPESGPLQERIERIEAQILMETIVRCRWNKSRAAKELGLSRVGLRAK
LERYGIAPDAGRETIQ
>Mature_496_residues
MNDLPPILVVDDEPRSVEVMARVLREEFDVHVANGAEAALAVLNAHWIQAIFCDQRMPGMTGVKLLADVRQRWPDIVRII
VTGYTDPDDMIEAINEAGIYQYITKPWHPDQLLLAARNATQLFAMQREHDRLSLELRLARPAVEKRLVRERALVRETYHF
EALVRTQGSPLNETCHQAAKAATFDIPVLIQGETGTGKELLARAIHYASQRASGPFLAVNCGAIPDTLLESELFGHKKGS
FTGAHANRRGLLEEADGGTLLLDEIGDISPAFQVKLLRFLQEGEIRAVGANESRRADVRILSATHRDLREEVRAGRFRED
LYFRLAAMALTMPPLRKRPGDVRVLADHLLDTLSARHGRRVHGFTAEAMTCLMDYDWPGNVRELQNEVLRMLVLTEGDLL
GADLLSPGVLHRVAGARASGHADGEAMESPTLSTPESGPLQERIERIEAQILMETIVRCRWNKSRAAKELGLSRVGLRAK
LERYGIAPDAGRETIQ

Specific function: Probable member of the two-component regulatory system involved in the regulation of the hydrogenase activity. HoxA is probably phosphorylated by a sensory component (which could be hoxX) and then acts in conjunction with sigma-54 as a transcriptional act

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=493, Percent_Identity=37.9310344827586, Blast_Score=325, Evalue=5e-90,
Organism=Escherichia coli, GI1790437, Length=478, Percent_Identity=38.7029288702929, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1788905, Length=400, Percent_Identity=40, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790299, Length=490, Percent_Identity=34.0816326530612, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI87082117, Length=329, Percent_Identity=43.4650455927052, Blast_Score=229, Evalue=4e-61,
Organism=Escherichia coli, GI87082152, Length=239, Percent_Identity=51.8828451882845, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1789233, Length=299, Percent_Identity=39.7993311036789, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1787583, Length=325, Percent_Identity=39.6923076923077, Blast_Score=215, Evalue=7e-57,
Organism=Escherichia coli, GI1789087, Length=321, Percent_Identity=39.8753894080997, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI87081872, Length=319, Percent_Identity=38.2445141065831, Blast_Score=194, Evalue=9e-51,
Organism=Escherichia coli, GI1786524, Length=217, Percent_Identity=47.926267281106, Blast_Score=192, Evalue=5e-50,
Organism=Escherichia coli, GI1789828, Length=381, Percent_Identity=31.496062992126, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081858, Length=223, Percent_Identity=36.7713004484305, Blast_Score=126, Evalue=3e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55253; Mature: 55253

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLPPILVVDDEPRSVEVMARVLREEFDVHVANGAEAALAVLNAHWIQAIFCDQRMPGM
CCCCCCEEEECCCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
TGVKLLADVRQRWPDIVRIIVTGYTDPDDMIEAINEAGIYQYITKPWHPDQLLLAARNAT
HHHHHHHHHHHHCHHHEEHHHCCCCCHHHHHHHHHHCCHHHHHCCCCCCCHHHEECCCHH
QLFAMQREHDRLSLELRLARPAVEKRLVRERALVRETYHFEALVRTQGSPLNETCHQAAK
HHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AATFDIPVLIQGETGTGKELLARAIHYASQRASGPFLAVNCGAIPDTLLESELFGHKKGS
HCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
FTGAHANRRGLLEEADGGTLLLDEIGDISPAFQVKLLRFLQEGEIRAVGANESRRADVRI
CCCCCCCCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEE
LSATHRDLREEVRAGRFREDLYFRLAAMALTMPPLRKRPGDVRVLADHLLDTLSARHGRR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCE
VHGFTAEAMTCLMDYDWPGNVRELQNEVLRMLVLTEGDLLGADLLSPGVLHRVAGARASG
ECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCCCC
HADGEAMESPTLSTPESGPLQERIERIEAQILMETIVRCRWNKSRAAKELGLSRVGLRAK
CCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHH
LERYGIAPDAGRETIQ
HHHCCCCCCCCCCCCC
>Mature Secondary Structure
MNDLPPILVVDDEPRSVEVMARVLREEFDVHVANGAEAALAVLNAHWIQAIFCDQRMPGM
CCCCCCEEEECCCCCHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
TGVKLLADVRQRWPDIVRIIVTGYTDPDDMIEAINEAGIYQYITKPWHPDQLLLAARNAT
HHHHHHHHHHHHCHHHEEHHHCCCCCHHHHHHHHHHCCHHHHHCCCCCCCHHHEECCCHH
QLFAMQREHDRLSLELRLARPAVEKRLVRERALVRETYHFEALVRTQGSPLNETCHQAAK
HHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
AATFDIPVLIQGETGTGKELLARAIHYASQRASGPFLAVNCGAIPDTLLESELFGHKKGS
HCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCC
FTGAHANRRGLLEEADGGTLLLDEIGDISPAFQVKLLRFLQEGEIRAVGANESRRADVRI
CCCCCCCCCCCCEECCCCEEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCCEEE
LSATHRDLREEVRAGRFREDLYFRLAAMALTMPPLRKRPGDVRVLADHLLDTLSARHGRR
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHHCCCE
VHGFTAEAMTCLMDYDWPGNVRELQNEVLRMLVLTEGDLLGADLLSPGVLHRVAGARASG
ECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHCCCCCC
HADGEAMESPTLSTPESGPLQERIERIEAQILMETIVRCRWNKSRAAKELGLSRVGLRAK
CCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHHHH
LERYGIAPDAGRETIQ
HHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2001989; 12948488 [H]