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The map label for this gene is metZ [H]

Identifier: 182678024

GI number: 182678024

Start: 1190599

End: 1191825

Strand: Direct

Name: metZ [H]

Synonym: Bind_1038

Alternate gene names: 182678024

Gene position: 1190599-1191825 (Clockwise)

Preceding gene: 182678022

Following gene: 182678025

Centisome position: 28.55

GC content: 60.07

Gene sequence:

>1227_bases
ATGCAGGATTTCAAGCCGCCGCGTGATCCAGAAGGCTGGCGGACGGCGACCCGGCTCGTTCATGGTGGAACGACGCGTTC
CCCTTTCGGTGAAACCGCCGAAGCGATCTTTTTGACCCAGGGCTATGTCTATCCGACGATGGAAGCGGCCGAAGCACGTT
TCAAGGGCGAGGAACCGGGTTTCGTCTATTCCCGCTACAATAATCCGACTAATGCCATGTTCGAGGAACGCATGGCCTTG
CTCGAGGGAGCCGAAGCGGCCCGTGCGACTGCGAGCGGCATGGCGGCGGTAACGGCCGCTTTGCTCGCGCCCTTGAAGGC
CGGTGATCATGTCGTCGCTTCGCGTGCCTTGTTCGGCTCCTGCCTCTATATTGTCGAGGAATTGCTGCCGCGCTACGGTA
TTGCCTCCACGCTGGTCGATGGCAAGGATTTCAAGGCCTGGAAAGACGCGCTGCGGCCGCAGACCCAGACCTTGTTTCTG
GAAAGCCCAACCAATCCAAGCCTCGAGGTCTATGATATTGCGGCTGTCGCGGCGATTGCCCATGCTCATGGCGCGCGCCT
CGTCGTCGATAATGCCTTCGCTTCGCCCATGTTGCAGAAGCCGCTGCAACTCGGCGCTGATTGCGTGGTCTATTCGGCGA
CCAAACATATCGATGGGCAGGGGCGTTGCCTTGGTGGTGTCGTCCTTTCGAGCAAGGATTTCATCGAAACGCATCTCCAG
ACCTATTTGCGCCAGACGGGTCCCGCTCTGTCTCCCTTCAACGCATGGATTCTTTTGAAGTCCCTGGAAACCCTGCCGCT
GCGTGTCCAGCAGCAGATGGCCAATGCGGCAAAAGTCGCCGATTTCCTGGCGGATCATCCGCTGATCGCGCGCTGTTTCT
ATCCTGGCCGCGCCGATCACCCGCAAGCCGAGATCGTCAAACGGCAAATGCTCGGGGGAGGCACGATGGTCGCCTTCGAG
GTCACGGGCGGCAAGCCGGCCGCCTTCGCCTTCGCCAATGCTCTGTCGATCATCAAGATTTCGAATAATCTCGGTGACGC
CAAAAGCCTCATCACGCATCCGGCCACGACGACGCATCAGCGTCTCACACCGGAGGCGCGTGCCACCATGGGCATCGGCG
AGGGACTGCTCAGACTGTCGGTGGGGCTCGAGGACGCGGAAGATCTGATCGCCGATTTGCAAGCCGCGCTCGCGGTGCTT
GGACAAAAGCAGCTTGCCGCTGAATAA

Upstream 100 bases:

>100_bases
TTGAGATGCGGTCCTGGCCCCAGTAAGCACGTCACACGATAGGTTTTCAAGCCGGTGACGAAGCTGCACGGCAGCAAGCT
ATTCTTCGAGGAGCCCACCC

Downstream 100 bases:

>100_bases
GGCAGGTGACGGGATGGCAAGAGGTGCCGCTTGTGCCTCTTTCCCAGCCTTTTCCTCAGCCGGCCCGTTGTGTTACCTTA
ACACCCAAACATTAAAGACG

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQDFKPPRDPEGWRTATRLVHGGTTRSPFGETAEAIFLTQGYVYPTMEAAEARFKGEEPGFVYSRYNNPTNAMFEERMAL
LEGAEAARATASGMAAVTAALLAPLKAGDHVVASRALFGSCLYIVEELLPRYGIASTLVDGKDFKAWKDALRPQTQTLFL
ESPTNPSLEVYDIAAVAAIAHAHGARLVVDNAFASPMLQKPLQLGADCVVYSATKHIDGQGRCLGGVVLSSKDFIETHLQ
TYLRQTGPALSPFNAWILLKSLETLPLRVQQQMANAAKVADFLADHPLIARCFYPGRADHPQAEIVKRQMLGGGTMVAFE
VTGGKPAAFAFANALSIIKISNNLGDAKSLITHPATTTHQRLTPEARATMGIGEGLLRLSVGLEDAEDLIADLQAALAVL
GQKQLAAE

Sequences:

>Translated_408_residues
MQDFKPPRDPEGWRTATRLVHGGTTRSPFGETAEAIFLTQGYVYPTMEAAEARFKGEEPGFVYSRYNNPTNAMFEERMAL
LEGAEAARATASGMAAVTAALLAPLKAGDHVVASRALFGSCLYIVEELLPRYGIASTLVDGKDFKAWKDALRPQTQTLFL
ESPTNPSLEVYDIAAVAAIAHAHGARLVVDNAFASPMLQKPLQLGADCVVYSATKHIDGQGRCLGGVVLSSKDFIETHLQ
TYLRQTGPALSPFNAWILLKSLETLPLRVQQQMANAAKVADFLADHPLIARCFYPGRADHPQAEIVKRQMLGGGTMVAFE
VTGGKPAAFAFANALSIIKISNNLGDAKSLITHPATTTHQRLTPEARATMGIGEGLLRLSVGLEDAEDLIADLQAALAVL
GQKQLAAE
>Mature_408_residues
MQDFKPPRDPEGWRTATRLVHGGTTRSPFGETAEAIFLTQGYVYPTMEAAEARFKGEEPGFVYSRYNNPTNAMFEERMAL
LEGAEAARATASGMAAVTAALLAPLKAGDHVVASRALFGSCLYIVEELLPRYGIASTLVDGKDFKAWKDALRPQTQTLFL
ESPTNPSLEVYDIAAVAAIAHAHGARLVVDNAFASPMLQKPLQLGADCVVYSATKHIDGQGRCLGGVVLSSKDFIETHLQ
TYLRQTGPALSPFNAWILLKSLETLPLRVQQQMANAAKVADFLADHPLIARCFYPGRADHPQAEIVKRQMLGGGTMVAFE
VTGGKPAAFAFANALSIIKISNNLGDAKSLITHPATTTHQRLTPEARATMGIGEGLLRLSVGLEDAEDLIADLQAALAVL
GQKQLAAE

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=329, Percent_Identity=36.7781155015198, Blast_Score=218, Evalue=7e-57,
Organism=Homo sapiens, GI299473759, Length=329, Percent_Identity=33.1306990881459, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI25453487, Length=328, Percent_Identity=30.1829268292683, Blast_Score=155, Evalue=5e-38,
Organism=Escherichia coli, GI1790375, Length=332, Percent_Identity=38.2530120481928, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1789383, Length=393, Percent_Identity=28.2442748091603, Blast_Score=137, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI17560056, Length=386, Percent_Identity=35.2331606217617, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17538055, Length=347, Percent_Identity=37.1757925072046, Blast_Score=216, Evalue=2e-56,
Organism=Caenorhabditis elegans, GI71980708, Length=370, Percent_Identity=34.8648648648649, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319307, Length=400, Percent_Identity=34.25, Blast_Score=213, Evalue=5e-56,
Organism=Saccharomyces cerevisiae, GI6323333, Length=415, Percent_Identity=33.7349397590361, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321254, Length=415, Percent_Identity=28.1927710843373, Blast_Score=143, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6321904, Length=400, Percent_Identity=29.5, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321170, Length=272, Percent_Identity=25.7352941176471, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI19922554, Length=349, Percent_Identity=37.2492836676218, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 43815; Mature: 43815

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00630 IMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDFKPPRDPEGWRTATRLVHGGTTRSPFGETAEAIFLTQGYVYPTMEAAEARFKGEEPG
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCC
FVYSRYNNPTNAMFEERMALLEGAEAARATASGMAAVTAALLAPLKAGDHVVASRALFGS
EEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CLYIVEELLPRYGIASTLVDGKDFKAWKDALRPQTQTLFLESPTNPSLEVYDIAAVAAIA
HHHHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHH
HAHGARLVVDNAFASPMLQKPLQLGADCVVYSATKHIDGQGRCLGGVVLSSKDFIETHLQ
HHCCCEEEEECHHHCHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEEEECCHHHHHHHHH
TYLRQTGPALSPFNAWILLKSLETLPLRVQQQMANAAKVADFLADHPLIARCFYPGRADH
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PQAEIVKRQMLGGGTMVAFEVTGGKPAAFAFANALSIIKISNNLGDAKSLITHPATTTHQ
CHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCHH
RLTPEARATMGIGEGLLRLSVGLEDAEDLIADLQAALAVLGQKQLAAE
HCCCHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure
MQDFKPPRDPEGWRTATRLVHGGTTRSPFGETAEAIFLTQGYVYPTMEAAEARFKGEEPG
CCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCC
FVYSRYNNPTNAMFEERMALLEGAEAARATASGMAAVTAALLAPLKAGDHVVASRALFGS
EEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
CLYIVEELLPRYGIASTLVDGKDFKAWKDALRPQTQTLFLESPTNPSLEVYDIAAVAAIA
HHHHHHHHHHHCCCHHHHCCCCCHHHHHHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHH
HAHGARLVVDNAFASPMLQKPLQLGADCVVYSATKHIDGQGRCLGGVVLSSKDFIETHLQ
HHCCCEEEEECHHHCHHHHHHHHCCCCEEEEEHHHCCCCCCCEEEEEEECCHHHHHHHHH
TYLRQTGPALSPFNAWILLKSLETLPLRVQQQMANAAKVADFLADHPLIARCFYPGRADH
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
PQAEIVKRQMLGGGTMVAFEVTGGKPAAFAFANALSIIKISNNLGDAKSLITHPATTTHQ
CHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHEEEEEECCCCCHHHHHHCCCCCCCHH
RLTPEARATMGIGEGLLRLSVGLEDAEDLIADLQAALAVLGQKQLAAE
HCCCHHHHHHHHCCCHHEEECCCCCHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]