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The map label for this gene is amiC [H]

Identifier: 182677925

GI number: 182677925

Start: 1067187

End: 1068704

Strand: Direct

Name: amiC [H]

Synonym: Bind_0933

Alternate gene names: 182677925

Gene position: 1067187-1068704 (Clockwise)

Preceding gene: 182677924

Following gene: 182677926

Centisome position: 25.59

GC content: 59.82

Gene sequence:

>1518_bases
TTGTCTGGGGTGCGCGGGCCGATGATGTTTTGGGACTGGAATGGCCATCCAGTGCGGGCTTCGCTGGATCGATTCTTCTC
TTTGCTTCAGGGCTTTTCCAAGCCGGGCAGGGCGTGGGCAGCGTGGCTTTTTTTCATTTTCATGAGCCCGGTTTCTTCGT
TTGCCGCCGAACAGGCGCCGGTTCAGGCCTCGGCGGCATGGCTCGAAACGCGTGGCGAGGGAAGCCGGCTTGTCTTTGAT
CTTTCCGCTCCGGTCGAGGTCAAGGCCATGGTGCTTTCCGCGCCTGATCGTGTGGTCATCGATCTGCCCGCCGTCGATTT
CATGTTCGCGCCACCCACTTTCGCGCCTGCCCCTGCGGCACTGGCTCGTCATCGCCGGGGGCGCCATGCTTCCACCGCGC
AGACTGTGGCCCCGAGCGGGCTCATTGCGGCTTATCGTTTCGGCTCTTTCGCACCCGGCCGATCGCGCATCGTCATCGAT
CTCAAGGAGCCAGCTCGCGTGATCCGAGCGGGCATGGAAGCCGCTCAAGACACTTCATCGTCTGGCCGGGCGCGCCTCGT
CATTGTCCTGGCCGCGACCGATCGGGCGAGTTTTCGGGCGGCGGCGCAAAATGGCCGGCAGGAAGTCGCGCTGGCATCCG
CTCAGGCCCCGGCGGCTTTGCCGGACAATGCGCCTATGATTGCGCCGAAGCCGGGACCCAATGTGGATCGGCCTGTCATT
GTCATCGATCCGGGCCACGGCGGCGTCGATAGCGGAGCGATGGCGGGTACGCTCGTCGAAAAAAACCTTGTGCGGGATTT
TGCCAAAAGCCTGGCCGATAAGCTTAACGCTAGCCGGCGCTATCAGATTATCATGACGCGGGAGGATGATATTTTCATTC
CCCTCGGAGAGCGGGTCAAGATTGCCCAGGCTCATCATGCCGATCTGTTCATTTCGATCCACGCCGATATTCTTTCAGAG
ACGGCGGATGTCGGTGGTGCCACCGTCTACACGGTTTCGGACAAGGCCTCGGATGCCGAGGCCGCACGTCTCGCCGATAA
GGAAAACCAGGCGGATCTTGTCGGCGGTCTCGAAAGTAAGGAAGAAACGCCGGAAATTACCGATATTCTATTCGATTTGA
CGCGGCGCGAGACGAGAGCCTACAGCCATGTTTTCGCGAGGACTTTAGTCGATTACTGGAAAGTCGTCGGCCGGCTCAAC
AAAAACCCTCAGCGCTCGGCGGGTTTTCGCGTTTTGAAGGCGCCTGATGTTCCTTCGGTTTTGCTCGAACTCGGCTATTT
GTCCAATGAGAATGATCACGCGGCCTTGGCTTCACCGGAATGGCGCGGAAAAGTTGTGACCAAGGTCGCCGAGGCGGTCG
ATCGATTTTTCGCCGCGCGCGGTCCGACTCAAGTTCAAGGGCAGTTTCCAGGACCAGGACAATTTCAAGGGGAAACGCAA
GCTCGGGCGAGGGGGCACGATTCCGCATCCATTCTGCGCGGTACAGCCACGACCGATCTTGCCCATGATGCGCATTGA

Upstream 100 bases:

>100_bases
TTCGAAAGCGATGATTCGGACCGGGCCGCGAACTTTCCTGTTCCTTGGGAACGGTCTTTGGAGAACCCAGCTTGGGCAAG
CCGGAAGGATGGGGATTGGG

Downstream 100 bases:

>100_bases
GACGATTCCACGAAGGAATCCAAACGGTTCATCGGGAGTGTTAGCGCATTGAAAGATGTCGGCAAGGAAAGACGTTGGTA
AGTCGCCGCAAGGTTCGGCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 505; Mature: 504

Protein sequence:

>505_residues
MSGVRGPMMFWDWNGHPVRASLDRFFSLLQGFSKPGRAWAAWLFFIFMSPVSSFAAEQAPVQASAAWLETRGEGSRLVFD
LSAPVEVKAMVLSAPDRVVIDLPAVDFMFAPPTFAPAPAALARHRRGRHASTAQTVAPSGLIAAYRFGSFAPGRSRIVID
LKEPARVIRAGMEAAQDTSSSGRARLVIVLAATDRASFRAAAQNGRQEVALASAQAPAALPDNAPMIAPKPGPNVDRPVI
VIDPGHGGVDSGAMAGTLVEKNLVRDFAKSLADKLNASRRYQIIMTREDDIFIPLGERVKIAQAHHADLFISIHADILSE
TADVGGATVYTVSDKASDAEAARLADKENQADLVGGLESKEETPEITDILFDLTRRETRAYSHVFARTLVDYWKVVGRLN
KNPQRSAGFRVLKAPDVPSVLLELGYLSNENDHAALASPEWRGKVVTKVAEAVDRFFAARGPTQVQGQFPGPGQFQGETQ
ARARGHDSASILRGTATTDLAHDAH

Sequences:

>Translated_505_residues
MSGVRGPMMFWDWNGHPVRASLDRFFSLLQGFSKPGRAWAAWLFFIFMSPVSSFAAEQAPVQASAAWLETRGEGSRLVFD
LSAPVEVKAMVLSAPDRVVIDLPAVDFMFAPPTFAPAPAALARHRRGRHASTAQTVAPSGLIAAYRFGSFAPGRSRIVID
LKEPARVIRAGMEAAQDTSSSGRARLVIVLAATDRASFRAAAQNGRQEVALASAQAPAALPDNAPMIAPKPGPNVDRPVI
VIDPGHGGVDSGAMAGTLVEKNLVRDFAKSLADKLNASRRYQIIMTREDDIFIPLGERVKIAQAHHADLFISIHADILSE
TADVGGATVYTVSDKASDAEAARLADKENQADLVGGLESKEETPEITDILFDLTRRETRAYSHVFARTLVDYWKVVGRLN
KNPQRSAGFRVLKAPDVPSVLLELGYLSNENDHAALASPEWRGKVVTKVAEAVDRFFAARGPTQVQGQFPGPGQFQGETQ
ARARGHDSASILRGTATTDLAHDAH
>Mature_504_residues
SGVRGPMMFWDWNGHPVRASLDRFFSLLQGFSKPGRAWAAWLFFIFMSPVSSFAAEQAPVQASAAWLETRGEGSRLVFDL
SAPVEVKAMVLSAPDRVVIDLPAVDFMFAPPTFAPAPAALARHRRGRHASTAQTVAPSGLIAAYRFGSFAPGRSRIVIDL
KEPARVIRAGMEAAQDTSSSGRARLVIVLAATDRASFRAAAQNGRQEVALASAQAPAALPDNAPMIAPKPGPNVDRPVIV
IDPGHGGVDSGAMAGTLVEKNLVRDFAKSLADKLNASRRYQIIMTREDDIFIPLGERVKIAQAHHADLFISIHADILSET
ADVGGATVYTVSDKASDAEAARLADKENQADLVGGLESKEETPEITDILFDLTRRETRAYSHVFARTLVDYWKVVGRLNK
NPQRSAGFRVLKAPDVPSVLLELGYLSNENDHAALASPEWRGKVVTKVAEAVDRFFAARGPTQVQGQFPGPGQFQGETQA
RARGHDSASILRGTATTDLAHDAH

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=438, Percent_Identity=31.5068493150685, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1788776, Length=233, Percent_Identity=37.3390557939914, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1790611, Length=230, Percent_Identity=30, Blast_Score=118, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 54412; Mature: 54281

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGVRGPMMFWDWNGHPVRASLDRFFSLLQGFSKPGRAWAAWLFFIFMSPVSSFAAEQAP
CCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VQASAAWLETRGEGSRLVFDLSAPVEVKAMVLSAPDRVVIDLPAVDFMFAPPTFAPAPAA
CHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCEEEEECCCCCEEECCCCCCCCHHH
LARHRRGRHASTAQTVAPSGLIAAYRFGSFAPGRSRIVIDLKEPARVIRAGMEAAQDTSS
HHHHHCCCCCCHHHHCCCCCCEEHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCC
SGRARLVIVLAATDRASFRAAAQNGRQEVALASAQAPAALPDNAPMIAPKPGPNVDRPVI
CCCEEEEEEEEECCCHHHHHHHHCCCHHEEEECCCCCCCCCCCCCEECCCCCCCCCCCEE
VIDPGHGGVDSGAMAGTLVEKNLVRDFAKSLADKLNASRRYQIIMTREDDIFIPLGERVK
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCEE
IAQAHHADLFISIHADILSETADVGGATVYTVSDKASDAEAARLADKENQADLVGGLESK
EEECCCCEEEEEEEHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHCCCCCC
EETPEITDILFDLTRRETRAYSHVFARTLVDYWKVVGRLNKNPQRSAGFRVLKAPDVPSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHH
LLELGYLSNENDHAALASPEWRGKVVTKVAEAVDRFFAARGPTQVQGQFPGPGQFQGETQ
HHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
ARARGHDSASILRGTATTDLAHDAH
HHHCCCCCHHHEECCCCCCCCCCCC
>Mature Secondary Structure 
SGVRGPMMFWDWNGHPVRASLDRFFSLLQGFSKPGRAWAAWLFFIFMSPVSSFAAEQAP
CCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
VQASAAWLETRGEGSRLVFDLSAPVEVKAMVLSAPDRVVIDLPAVDFMFAPPTFAPAPAA
CHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEECCCEEEEECCCCCEEECCCCCCCCHHH
LARHRRGRHASTAQTVAPSGLIAAYRFGSFAPGRSRIVIDLKEPARVIRAGMEAAQDTSS
HHHHHCCCCCCHHHHCCCCCCEEHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCC
SGRARLVIVLAATDRASFRAAAQNGRQEVALASAQAPAALPDNAPMIAPKPGPNVDRPVI
CCCEEEEEEEEECCCHHHHHHHHCCCHHEEEECCCCCCCCCCCCCEECCCCCCCCCCCEE
VIDPGHGGVDSGAMAGTLVEKNLVRDFAKSLADKLNASRRYQIIMTREDDIFIPLGERVK
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCEEEECCCCEE
IAQAHHADLFISIHADILSETADVGGATVYTVSDKASDAEAARLADKENQADLVGGLESK
EEECCCCEEEEEEEHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHCCCCCC
EETPEITDILFDLTRRETRAYSHVFARTLVDYWKVVGRLNKNPQRSAGFRVLKAPDVPSV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHH
LLELGYLSNENDHAALASPEWRGKVVTKVAEAVDRFFAARGPTQVQGQFPGPGQFQGETQ
HHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
ARARGHDSASILRGTATTDLAHDAH
HHHCCCCCHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]