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The map label for this gene is capI [H]

Identifier: 182677789

GI number: 182677789

Start: 889086

End: 890120

Strand: Direct

Name: capI [H]

Synonym: Bind_0796

Alternate gene names: 182677789

Gene position: 889086-890120 (Clockwise)

Preceding gene: 182677785

Following gene: 182677790

Centisome position: 21.32

GC content: 54.2

Gene sequence:

>1035_bases
ATGAATGATGAGCAAGCGCTGATCCTTGTGACCGGTGTCGCGGGTTTCATCGGGTCTTTCCTAGCCGCGCGGCTTCTCGA
TGAGAATCGGGAAGTGGTCGGAATCGACAATATGAATGCCTATTATGATCCGGCTTTGAAACGGGCGCGCCTCGCACAAC
TCTCGTCGCGTGCGGGCTTTCGGTTTCTTGAAGGCGATCTCGTCGATACGGATTTCATGCGCGCCGCGTTCACCGAGACG
CGGCCCAAAATCGTCGTCAATCTCGCGGCGCAGGCGGGGGTGCGTTATTCACTCGAAAACCCGCGTTCCTATGTCGATTC
CAATATTGTCGGCTTTCTCAATATTCTGGAAAATTGCCGTGCGATGGGAGTCGAGCATCTGGTCTATGCCTCGTCGAGTT
CTGTCTATGGCGCCAACCCGACCATGCCCTTTTCCACGCGGGACAATGTCGATCATCCCGTAAGCCTTTATGCTGCCTCG
AAAAAGGCTAATGAGTTGATGGCGCATTCTTATGCGCATCTCTATGGGCTGCCTGTGACGGGCCTTCGGTTTTTTACCGT
CTATGGCCCGTGGGGACGCCCTGACATGGCCTATTTCATCTTCACGCGGAAAATTCTCGCTGGCGAACCGATCGATGTGT
TTAATCACGGCGATCTCTCGCGTGATTTCACTTATATCGACGATATCGTCGATGGCGTGCGCAAAGTCATGGATCACGTG
CCGAAAGGCGATCCGAATTGGGCGACCAATGGGGCTTCACCGGCGACCAGCACCGCGCCCTACCAGCTTTATAATATTGG
CAATAACCGGCCGGAACGCCTGCTGGATATGATCGAAACCCTTGAGACCCTGTTGGGCCGTAAGGCGGAAAAACGTTTTC
TGCCCCTCCAACCGGGTGATGTCCTTGCGACTTGGGCCGATATAGACGATCTCCAAAAGGATACGGGCTTTGCGCCGAAA
ACCACGCTTGCTCAGGGCCTTTCGCATTTTGTTGATTGGTATCGCGATTTCTACAAGATCGCCGGCAAGAGTTAG

Upstream 100 bases:

>100_bases
ACCGAAAGTCGATGTTTTTGGATAAACGCGAGCCGTTGTCAAAAAGCTGGAGCGAGGGACAATCCATCATGGCGCTTTCT
CTCACTGGGGGCAGGCCATT

Downstream 100 bases:

>100_bases
GGCATGATGGGCGGATGCAGCCGTCAGAAAAAATCAGCCGACAGGAACGCGGCGTGCAGATAGAGTCTAGAATCGAGAAA
AGCGCCCAGGATCACAACGG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNDEQALILVTGVAGFIGSFLAARLLDENREVVGIDNMNAYYDPALKRARLAQLSSRAGFRFLEGDLVDTDFMRAAFTET
RPKIVVNLAAQAGVRYSLENPRSYVDSNIVGFLNILENCRAMGVEHLVYASSSSVYGANPTMPFSTRDNVDHPVSLYAAS
KKANELMAHSYAHLYGLPVTGLRFFTVYGPWGRPDMAYFIFTRKILAGEPIDVFNHGDLSRDFTYIDDIVDGVRKVMDHV
PKGDPNWATNGASPATSTAPYQLYNIGNNRPERLLDMIETLETLLGRKAEKRFLPLQPGDVLATWADIDDLQKDTGFAPK
TTLAQGLSHFVDWYRDFYKIAGKS

Sequences:

>Translated_344_residues
MNDEQALILVTGVAGFIGSFLAARLLDENREVVGIDNMNAYYDPALKRARLAQLSSRAGFRFLEGDLVDTDFMRAAFTET
RPKIVVNLAAQAGVRYSLENPRSYVDSNIVGFLNILENCRAMGVEHLVYASSSSVYGANPTMPFSTRDNVDHPVSLYAAS
KKANELMAHSYAHLYGLPVTGLRFFTVYGPWGRPDMAYFIFTRKILAGEPIDVFNHGDLSRDFTYIDDIVDGVRKVMDHV
PKGDPNWATNGASPATSTAPYQLYNIGNNRPERLLDMIETLETLLGRKAEKRFLPLQPGDVLATWADIDDLQKDTGFAPK
TTLAQGLSHFVDWYRDFYKIAGKS
>Mature_344_residues
MNDEQALILVTGVAGFIGSFLAARLLDENREVVGIDNMNAYYDPALKRARLAQLSSRAGFRFLEGDLVDTDFMRAAFTET
RPKIVVNLAAQAGVRYSLENPRSYVDSNIVGFLNILENCRAMGVEHLVYASSSSVYGANPTMPFSTRDNVDHPVSLYAAS
KKANELMAHSYAHLYGLPVTGLRFFTVYGPWGRPDMAYFIFTRKILAGEPIDVFNHGDLSRDFTYIDDIVDGVRKVMDHV
PKGDPNWATNGASPATSTAPYQLYNIGNNRPERLLDMIETLETLLGRKAEKRFLPLQPGDVLATWADIDDLQKDTGFAPK
TTLAQGLSHFVDWYRDFYKIAGKS

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=345, Percent_Identity=24.9275362318841, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=26.5671641791045, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI56237023, Length=346, Percent_Identity=24.8554913294798, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI56118217, Length=346, Percent_Identity=24.8554913294798, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI189083684, Length=346, Percent_Identity=24.8554913294798, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI48994969, Length=349, Percent_Identity=28.6532951289398, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788353, Length=357, Percent_Identity=25.7703081232493, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1786974, Length=336, Percent_Identity=23.5119047619048, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1788366, Length=182, Percent_Identity=30.7692307692308, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=26.0744985673352, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=25.6410256410256, Blast_Score=97, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=346, Percent_Identity=26.5895953757225, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=346, Percent_Identity=24.8554913294798, Blast_Score=88, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=340, Percent_Identity=23.8235294117647, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319493, Length=258, Percent_Identity=28.2945736434109, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21356223, Length=336, Percent_Identity=24.4047619047619, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI19923002, Length=331, Percent_Identity=27.190332326284, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38326; Mature: 38326

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDEQALILVTGVAGFIGSFLAARLLDENREVVGIDNMNAYYDPALKRARLAQLSSRAGF
CCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCC
RFLEGDLVDTDFMRAAFTETRPKIVVNLAAQAGVRYSLENPRSYVDSNIVGFLNILENCR
EEECCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHH
AMGVEHLVYASSSSVYGANPTMPFSTRDNVDHPVSLYAASKKANELMAHSYAHLYGLPVT
HHCHHHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLRFFTVYGPWGRPDMAYFIFTRKILAGEPIDVFNHGDLSRDFTYIDDIVDGVRKVMDHV
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
PKGDPNWATNGASPATSTAPYQLYNIGNNRPERLLDMIETLETLLGRKAEKRFLPLQPGD
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
VLATWADIDDLQKDTGFAPKTTLAQGLSHFVDWYRDFYKIAGKS
CEEEHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNDEQALILVTGVAGFIGSFLAARLLDENREVVGIDNMNAYYDPALKRARLAQLSSRAGF
CCCCCEEEEEECHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCC
RFLEGDLVDTDFMRAAFTETRPKIVVNLAAQAGVRYSLENPRSYVDSNIVGFLNILENCR
EEECCCCCCHHHHHHHHHCCCCEEEEEEHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHH
AMGVEHLVYASSSSVYGANPTMPFSTRDNVDHPVSLYAASKKANELMAHSYAHLYGLPVT
HHCHHHEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCC
GLRFFTVYGPWGRPDMAYFIFTRKILAGEPIDVFNHGDLSRDFTYIDDIVDGVRKVMDHV
CEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHC
PKGDPNWATNGASPATSTAPYQLYNIGNNRPERLLDMIETLETLLGRKAEKRFLPLQPGD
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCC
VLATWADIDDLQKDTGFAPKTTLAQGLSHFVDWYRDFYKIAGKS
CEEEHHCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]