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The map label for this gene is murD [H]

Identifier: 182677683

GI number: 182677683

Start: 771775

End: 773163

Strand: Direct

Name: murD [H]

Synonym: Bind_0690

Alternate gene names: 182677683

Gene position: 771775-773163 (Clockwise)

Preceding gene: 182677682

Following gene: 182677684

Centisome position: 18.51

GC content: 60.69

Gene sequence:

>1389_bases
ATGACACCGGTCACAGCATTCAAGGGCAAGCGCCTTGCCTTGTTTGGTCTCGGCGGGTCGGGGCTCGTCAGTGCGCAAGC
GCTGAAAGCGGGCGGCGCCGAGGTCGCCGTTTGGGACGACAAGGACAGCGCGCGTGAAACCGCCAAAGCCAAGGGGCTTG
AGGTTCTCGATCTTTCGCAAGCGGACTGGAGCCAGTTTTGCGCCTTGGTGCTGGCTCCTGGCGTCCCCCTGACGCATCCC
AAACCGCATTGGACGGTCGAAAAGGCACGCGCCGCCGGCATCGAAATCATTGGCGATATCGAACTATTTTGCCGTGAACG
GCGCTTGATCGCCCCGAAATCGCCTTTCGTCGCGATCACCGGCACCAATGGGAAATCGACGACGACCGCGCTTGTCGCCC
ATCTGTTCCAGACCTTTGGCTATGAGGTCGCGATCGGCGGCAATATCGGCACGCCGATCCTTGCCTTGGAGCCTCCATCG
CCGACCCGTGTCCATGTGATCGAGTGTTCCTCGTTCCAGATCGATCTGACGCCGACGCTTGATCCTTGGGTCGGCGTGCT
GCTCAACATCACGCCCGATCATCTCGATCGTCATGGCACGATGGAAAATTATGCGGCGATCAAGGCGCGGCTCGTCATGG
GCGCCGATAAGGCCGTCATCTGTCTCGATGATGAGCCTTGCGTCGCTATTGCTGAAACTCTGCGGCAAAAGCACAAGCCC
AGCACTTTCGTTTCGATCAGCCATCCAGAATTGCGCGACGGCATTATTCTCGATGGGACGACGCTCGTGCGTCATGTCGA
TGGCGGCTCGGTGGTGATCGCTGATCTTGCTGGTATTGCCTCCTTGCGGGGCCGGCACAATGGCCAGAATGCCGCCGCCG
CTGTCGCGGCTCTCGGCGCGCATGGATATAATCTCGATCTCGTGCGCAAGGGGCTTGAGAGCTTTCCCGGCCTGCCGCAT
CGCCTCGAAGAAGTCGGACGCCTCGGCAATGTTCTTTTCGTCAATGATTCCAAGGCGACCAATGCCGATGCGGCGGAAAA
GGCCTTGTTGTCCTTCGAGAATATTTTCTGGATTCTCGGTGGCAAGCCGAAGGCCGGTGGCATCGATCCTCTGCGGCCTT
TATTCGGTAAGATTGCTAAAGGCTATCTGATCGGCGAGGCGAGCGAGGCTTTCGCGGCGACGATCGGCGATGCCTTTCCA
CTCGAACGCTGTGGCACGCTCGATGTCGCCACCGCCGCCGCCGCGCGTGACGCGGACGCGAGCGCGCTGCCGGACCCGAT
CGTGCTGCTTTCGCCGGCTTGCGCCTCCTATGACCAATTCCCCGATTTCGAAAAGCGCGGCGACAAGTTTCGCGAGCTCG
CCCGCGCCATCATCGGCCGGTCCGGTTGA

Upstream 100 bases:

>100_bases
AACAACTCGGCTGGTCCGAGCCGCAGGTCGTGGTCCGTTTCTGGATCATTGCCTTCGTGCTCGCGCTCGTCGGCCTTTCG
ACCTTGAAGCTGAGGTAGGC

Downstream 100 bases:

>100_bases
TCCGAACCTGCACAAACCCTTGATCCTCACCCTTCTTCCGAGACGGAGGGCACAGCATGATAAGCAGGGCAGAGCGTTCG
CCTTTGGCCAATTGGTGGTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTPVTAFKGKRLALFGLGGSGLVSAQALKAGGAEVAVWDDKDSARETAKAKGLEVLDLSQADWSQFCALVLAPGVPLTHP
KPHWTVEKARAAGIEIIGDIELFCRERRLIAPKSPFVAITGTNGKSTTTALVAHLFQTFGYEVAIGGNIGTPILALEPPS
PTRVHVIECSSFQIDLTPTLDPWVGVLLNITPDHLDRHGTMENYAAIKARLVMGADKAVICLDDEPCVAIAETLRQKHKP
STFVSISHPELRDGIILDGTTLVRHVDGGSVVIADLAGIASLRGRHNGQNAAAAVAALGAHGYNLDLVRKGLESFPGLPH
RLEEVGRLGNVLFVNDSKATNADAAEKALLSFENIFWILGGKPKAGGIDPLRPLFGKIAKGYLIGEASEAFAATIGDAFP
LERCGTLDVATAAAARDADASALPDPIVLLSPACASYDQFPDFEKRGDKFRELARAIIGRSG

Sequences:

>Translated_462_residues
MTPVTAFKGKRLALFGLGGSGLVSAQALKAGGAEVAVWDDKDSARETAKAKGLEVLDLSQADWSQFCALVLAPGVPLTHP
KPHWTVEKARAAGIEIIGDIELFCRERRLIAPKSPFVAITGTNGKSTTTALVAHLFQTFGYEVAIGGNIGTPILALEPPS
PTRVHVIECSSFQIDLTPTLDPWVGVLLNITPDHLDRHGTMENYAAIKARLVMGADKAVICLDDEPCVAIAETLRQKHKP
STFVSISHPELRDGIILDGTTLVRHVDGGSVVIADLAGIASLRGRHNGQNAAAAVAALGAHGYNLDLVRKGLESFPGLPH
RLEEVGRLGNVLFVNDSKATNADAAEKALLSFENIFWILGGKPKAGGIDPLRPLFGKIAKGYLIGEASEAFAATIGDAFP
LERCGTLDVATAAAARDADASALPDPIVLLSPACASYDQFPDFEKRGDKFRELARAIIGRSG
>Mature_461_residues
TPVTAFKGKRLALFGLGGSGLVSAQALKAGGAEVAVWDDKDSARETAKAKGLEVLDLSQADWSQFCALVLAPGVPLTHPK
PHWTVEKARAAGIEIIGDIELFCRERRLIAPKSPFVAITGTNGKSTTTALVAHLFQTFGYEVAIGGNIGTPILALEPPSP
TRVHVIECSSFQIDLTPTLDPWVGVLLNITPDHLDRHGTMENYAAIKARLVMGADKAVICLDDEPCVAIAETLRQKHKPS
TFVSISHPELRDGIILDGTTLVRHVDGGSVVIADLAGIASLRGRHNGQNAAAAVAALGAHGYNLDLVRKGLESFPGLPHR
LEEVGRLGNVLFVNDSKATNADAAEKALLSFENIFWILGGKPKAGGIDPLRPLFGKIAKGYLIGEASEAFAATIGDAFPL
ERCGTLDVATAAAARDADASALPDPIVLLSPACASYDQFPDFEKRGDKFRELARAIIGRSG

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=474, Percent_Identity=34.5991561181435, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013116
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF07991 IlvN; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 48847; Mature: 48716

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPVTAFKGKRLALFGLGGSGLVSAQALKAGGAEVAVWDDKDSARETAKAKGLEVLDLSQ
CCCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEECCC
ADWSQFCALVLAPGVPLTHPKPHWTVEKARAAGIEIIGDIELFCRERRLIAPKSPFVAIT
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEEEECHHHHHHHCCCCCCCCCEEEEE
GTNGKSTTTALVAHLFQTFGYEVAIGGNIGTPILALEPPSPTRVHVIECSSFQIDLTPTL
CCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEEEECCEEEEECCCC
DPWVGVLLNITPDHLDRHGTMENYAAIKARLVMGADKAVICLDDEPCVAIAETLRQKHKP
CHHHHEEEECCHHHHHCCCCHHHHHHHHEEEEECCCCEEEEECCCCHHHHHHHHHHHCCC
STFVSISHPELRDGIILDGTTLVRHVDGGSVVIADLAGIASLRGRHNGQNAAAAVAALGA
CEEEEECCCCCCCCEEECCCEEEEEECCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHCC
HGYNLDLVRKGLESFPGLPHRLEEVGRLGNVLFVNDSKATNADAAEKALLSFENIFWILG
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEC
GKPKAGGIDPLRPLFGKIAKGYLIGEASEAFAATIGDAFPLERCGTLDVATAAAARDADA
CCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHHCCCCCHHHCCCCHHHHHHHHCCCCC
SALPDPIVLLSPACASYDQFPDFEKRGDKFRELARAIIGRSG
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPVTAFKGKRLALFGLGGSGLVSAQALKAGGAEVAVWDDKDSARETAKAKGLEVLDLSQ
CCCCCCCCCEEEEEECCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEECCC
ADWSQFCALVLAPGVPLTHPKPHWTVEKARAAGIEIIGDIELFCRERRLIAPKSPFVAIT
CCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCEEEEECHHHHHHHCCCCCCCCCEEEEE
GTNGKSTTTALVAHLFQTFGYEVAIGGNIGTPILALEPPSPTRVHVIECSSFQIDLTPTL
CCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCEEEECCCCCCEEEEEEECCEEEEECCCC
DPWVGVLLNITPDHLDRHGTMENYAAIKARLVMGADKAVICLDDEPCVAIAETLRQKHKP
CHHHHEEEECCHHHHHCCCCHHHHHHHHEEEEECCCCEEEEECCCCHHHHHHHHHHHCCC
STFVSISHPELRDGIILDGTTLVRHVDGGSVVIADLAGIASLRGRHNGQNAAAAVAALGA
CEEEEECCCCCCCCEEECCCEEEEEECCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHCC
HGYNLDLVRKGLESFPGLPHRLEEVGRLGNVLFVNDSKATNADAAEKALLSFENIFWILG
CCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCEEEEEC
GKPKAGGIDPLRPLFGKIAKGYLIGEASEAFAATIGDAFPLERCGTLDVATAAAARDADA
CCCCCCCCCHHHHHHHHHHCCEEEECCHHHHHHHHCCCCCHHHCCCCHHHHHHHHCCCCC
SALPDPIVLLSPACASYDQFPDFEKRGDKFRELARAIIGRSG
CCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]