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The map label for this gene is murF [H]

Identifier: 182677681

GI number: 182677681

Start: 769091

End: 770551

Strand: Direct

Name: murF [H]

Synonym: Bind_0688

Alternate gene names: 182677681

Gene position: 769091-770551 (Clockwise)

Preceding gene: 182677680

Following gene: 182677682

Centisome position: 18.44

GC content: 62.15

Gene sequence:

>1461_bases
ATGAACGAGATTTTGATGCCCGAAAGCGTCGATAAGGAGATTTTGTGGACGCCGCTCGGTCTCGTCACGCCTTTGCAGGC
CCGTGTCAGTGGTCATGTTCCGTTGCGCCCGGCGCGCGGGATTTCCATCGATACGCGGACATTGCGCGAGGATGATCTCT
TCTTCGCCATCAAGGGTGATAACAGCGATGGCCACGATTACGTCCCCGCGGCCTTCGCCAAGGGCGCCATGGCGGCGGTC
GTCGATGAAGCTCATGCCGACCGTCTAGCAGCGCATGGCCCTCTCTATGTCGTGCATGAGGTTCTGCCGGCGCTCGAACG
CCTTGGCCAGGCGGCGCGCCGGCGCGTCGAGGCTCGCGTCGTCGCGGTGACGGGATCGGTCGGCAAAACCTCGACCAAAG
AGGCTCTGCGGCTGGTCTTGACCCAGGGCGGCTCTGTCCATGCCTCGGTCGCGTCTTATAATAATCATTGGGGCGTGCCG
TTGACCCTCGCGCGCATGCCGCGCGACGCGCGTTTCGGGATTTTCGAGATCGGCATGAATCATCCGGGCGAAATCACTCC
GCTCGTTGGCATGGTGCGACCGCATGTTGCGATCATCACCACGGTGGCTCCGGTCCATCTCGAATATTTCGAGAATGTCG
AGGCGATCGCCGACGCAAAGGCTGAGATTTTCTCAGGATTGGTGCCGGGCGGCGTCGCGATCATCCATCGTGATATTCCG
CAGTTCGAGCGGCTGGCCGAGGCGGCGAGACATTCGCCCGCCGGATTGATCGCCAGTTTCGGCACGCATGAGAAAGCCGA
TGCGCGTCTTCTCGATCTGACACCATCATCCGATCATTCGATGGTCAAGGCCTCCATCCAGGGAGAGACCCTGACTTATC
GCCTTGGCGCGCCGGGCCGGCATTTCGCGGAAAATTCCCTGGCTGTCCTGCTCGCCGCCAAGGCCTGCGGCGTCCCGCTC
GAGGATGCCGCCGCTGTCCTTGCCTTTTGCGCGCCCCAGGCCGGGCGCGGCCAGCAACTGGTCCTGGAAGCGGGTGATGG
CCCCTTCCGTCTCATTGATGAAAGCTATAATGCCAATCCGGCCTCCATGCGCGCGGCGTTGGAAATGGCCGGCACTTTGC
CGTTGGAAGGGGTCGGCCGGCGCATCGCCATCCTGGGTGATATGCTGGAATTAGGCCCCCAGGCTCCGGATTTACATGCT
CAATTGGCTGATATCCTGCGTGAGGCATGTTTCGATCTCGTCTTCGCGGCGGGTCCCTTGATGCGGCATCTCTATGACGC
GCTGCCGAGCGGTCTGCGCGGGGAATGGCGCGCGAAGGCCGAGGATCTCGTGCCGATCGTCGGCGCCACGGTCCAGCGCA
ATGATATTGTGCTTGTCAAAGGCTCGAACGGCAGCCGCATGAAACTGCTGGTCGAAAGCCTCAAGGACAAGGCGCGGCGG
GCGGGAGCGGAAACGGTCTAA

Upstream 100 bases:

>100_bases
TTGCTGGCAAGGGCCATGAAACCGGCCAGATCCTGGCTGACAGGACGCTGCCCTTTTCCGATCGCGATTGCGCCCGCGCG
GCGCTTGAGGACCATGCTGC

Downstream 100 bases:

>100_bases
ACCGTCTTCGCTAAAATTTTTGAGTTCGTGTGTTTTTGTCGCAGAGCCGGGCTGCTTTCCCAAAAAGCTGTTGGGGTTCA
AAACGCATCAAACCAAAGCA

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MNEILMPESVDKEILWTPLGLVTPLQARVSGHVPLRPARGISIDTRTLREDDLFFAIKGDNSDGHDYVPAAFAKGAMAAV
VDEAHADRLAAHGPLYVVHEVLPALERLGQAARRRVEARVVAVTGSVGKTSTKEALRLVLTQGGSVHASVASYNNHWGVP
LTLARMPRDARFGIFEIGMNHPGEITPLVGMVRPHVAIITTVAPVHLEYFENVEAIADAKAEIFSGLVPGGVAIIHRDIP
QFERLAEAARHSPAGLIASFGTHEKADARLLDLTPSSDHSMVKASIQGETLTYRLGAPGRHFAENSLAVLLAAKACGVPL
EDAAAVLAFCAPQAGRGQQLVLEAGDGPFRLIDESYNANPASMRAALEMAGTLPLEGVGRRIAILGDMLELGPQAPDLHA
QLADILREACFDLVFAAGPLMRHLYDALPSGLRGEWRAKAEDLVPIVGATVQRNDIVLVKGSNGSRMKLLVESLKDKARR
AGAETV

Sequences:

>Translated_486_residues
MNEILMPESVDKEILWTPLGLVTPLQARVSGHVPLRPARGISIDTRTLREDDLFFAIKGDNSDGHDYVPAAFAKGAMAAV
VDEAHADRLAAHGPLYVVHEVLPALERLGQAARRRVEARVVAVTGSVGKTSTKEALRLVLTQGGSVHASVASYNNHWGVP
LTLARMPRDARFGIFEIGMNHPGEITPLVGMVRPHVAIITTVAPVHLEYFENVEAIADAKAEIFSGLVPGGVAIIHRDIP
QFERLAEAARHSPAGLIASFGTHEKADARLLDLTPSSDHSMVKASIQGETLTYRLGAPGRHFAENSLAVLLAAKACGVPL
EDAAAVLAFCAPQAGRGQQLVLEAGDGPFRLIDESYNANPASMRAALEMAGTLPLEGVGRRIAILGDMLELGPQAPDLHA
QLADILREACFDLVFAAGPLMRHLYDALPSGLRGEWRAKAEDLVPIVGATVQRNDIVLVKGSNGSRMKLLVESLKDKARR
AGAETV
>Mature_486_residues
MNEILMPESVDKEILWTPLGLVTPLQARVSGHVPLRPARGISIDTRTLREDDLFFAIKGDNSDGHDYVPAAFAKGAMAAV
VDEAHADRLAAHGPLYVVHEVLPALERLGQAARRRVEARVVAVTGSVGKTSTKEALRLVLTQGGSVHASVASYNNHWGVP
LTLARMPRDARFGIFEIGMNHPGEITPLVGMVRPHVAIITTVAPVHLEYFENVEAIADAKAEIFSGLVPGGVAIIHRDIP
QFERLAEAARHSPAGLIASFGTHEKADARLLDLTPSSDHSMVKASIQGETLTYRLGAPGRHFAENSLAVLLAAKACGVPL
EDAAAVLAFCAPQAGRGQQLVLEAGDGPFRLIDESYNANPASMRAALEMAGTLPLEGVGRRIAILGDMLELGPQAPDLHA
QLADILREACFDLVFAAGPLMRHLYDALPSGLRGEWRAKAEDLVPIVGATVQRNDIVLVKGSNGSRMKLLVESLKDKARR
AGAETV

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=450, Percent_Identity=32.2222222222222, Blast_Score=169, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51918; Mature: 51918

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEILMPESVDKEILWTPLGLVTPLQARVSGHVPLRPARGISIDTRTLREDDLFFAIKGD
CCCCCCCCCCCCHHEECCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
NSDGHDYVPAAFAKGAMAAVVDEAHADRLAAHGPLYVVHEVLPALERLGQAARRRVEARV
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
VAVTGSVGKTSTKEALRLVLTQGGSVHASVASYNNHWGVPLTLARMPRDARFGIFEIGMN
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCEEEEECCC
HPGEITPLVGMVRPHVAIITTVAPVHLEYFENVEAIADAKAEIFSGLVPGGVAIIHRDIP
CCCCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCH
QFERLAEAARHSPAGLIASFGTHEKADARLLDLTPSSDHSMVKASIQGETLTYRLGAPGR
HHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCC
HFAENSLAVLLAAKACGVPLEDAAAVLAFCAPQAGRGQQLVLEAGDGPFRLIDESYNANP
CCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCCCCH
ASMRAALEMAGTLPLEGVGRRIAILGDMLELGPQAPDLHAQLADILREACFDLVFAAGPL
HHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHH
MRHLYDALPSGLRGEWRAKAEDLVPIVGATVQRNDIVLVKGSNGSRMKLLVESLKDKARR
HHHHHHHCCCCCCCCCCCCHHHCHHHHCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHH
AGAETV
CCCCCC
>Mature Secondary Structure
MNEILMPESVDKEILWTPLGLVTPLQARVSGHVPLRPARGISIDTRTLREDDLFFAIKGD
CCCCCCCCCCCCHHEECCCHHHCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECC
NSDGHDYVPAAFAKGAMAAVVDEAHADRLAAHGPLYVVHEVLPALERLGQAARRRVEARV
CCCCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
VAVTGSVGKTSTKEALRLVLTQGGSVHASVASYNNHWGVPLTLARMPRDARFGIFEIGMN
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEECCCCCCCCEEEEECCC
HPGEITPLVGMVRPHVAIITTVAPVHLEYFENVEAIADAKAEIFSGLVPGGVAIIHRDIP
CCCCCCHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCH
QFERLAEAARHSPAGLIASFGTHEKADARLLDLTPSSDHSMVKASIQGETLTYRLGAPGR
HHHHHHHHHCCCCCCCEEECCCCCCCCCEEEEECCCCCCCEEEEEECCCEEEEEECCCCC
HFAENSLAVLLAAKACGVPLEDAAAVLAFCAPQAGRGQQLVLEAGDGPFRLIDESYNANP
CCCCCCCEEEEEHHHCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCEEEECCCCCCCH
ASMRAALEMAGTLPLEGVGRRIAILGDMLELGPQAPDLHAQLADILREACFDLVFAAGPL
HHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCHH
MRHLYDALPSGLRGEWRAKAEDLVPIVGATVQRNDIVLVKGSNGSRMKLLVESLKDKARR
HHHHHHHCCCCCCCCCCCCHHHCHHHHCCEEECCCEEEEECCCCCHHHHHHHHHHHHHHH
AGAETV
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]