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The map label for this gene is thiD [H]

Identifier: 182682141

GI number: 182682141

Start: 1794110

End: 1794919

Strand: Direct

Name: thiD [H]

Synonym: XfasM23_1619

Alternate gene names: 182682141

Gene position: 1794110-1794919 (Clockwise)

Preceding gene: 182682138

Following gene: 182682144

Centisome position: 70.75

GC content: 54.94

Gene sequence:

>810_bases
ATGAACATCCCCAACATTATCTCCGCACTCACTATCGCCGGCTCGGACACAGGCGGTGGCGCAGGCATCCAGGCCGATCT
AAAAACGTTCGCCGCACATCATATACACGGACTCTCTGCGATTACCGTGCTAACTGCGCAGCACACCCGCGGGGTGAATG
CAGTGCATATACCGCCGCTGGAATTTATCGCAACACAAATCGACACCTGCTTCTCTGACTTCAATGTACACGCCGTCAAA
CTCGGCATGCTCGCCAATGCCAAAGTCATCACACTGGTCGCCGAAGCCTTGCAACGGCATCAACCACCTTGCATCGTCCT
AGATCCCGTGATGATTGCTACCAGCGGCAGCACACTCCTTGAAGACAGCGCATTGGAGGCTTTACGCACCCAGCTGCTGC
CATTAGCCACCTTAATCACACCTAACATTCCAGAAGCCGAGCGACTCCTCAATCGTCATATCAACAGCGGCAGCGATGCC
GACACCGCAGCAGCATCATTACTGAAACTTGGCGCTCACGCGGTACTACTTAAGGGGGGACATTTGAACGAAGGCAACAG
TGTGATTGATCGCTTCGACAACGGCACATTACGCGAGCGCTTCAGCAATCCACGCTTAGCATTGAATGCCCACGGCACCG
GCTGCACCCTATCCTCAGCGATCACCGCCCAACTGTGCCTTGGTCTGAACCTGCATCAAGCCTGCAAAGCAGGCATTGAC
TACGTTGCACGCGGATTGCACGCGGGCTACCGACCTGGACGCGGCAACATCACCGTACTCAACCACATACCACCAAAGCA
TACGGAATAA

Upstream 100 bases:

>100_bases
CATGCCCTCCACCTGGAATGGCAGATCATGCGACACGCACTACACCCCATCACCACAGCAACAACGTGCATGACATAAAA
ACCCATTGCACAATGACCAC

Downstream 100 bases:

>100_bases
ACACTCTAAAGACTGATAGCCAACAATACACATAACATGCCCATCCACTACCATCAGTGCGGATGCAGACATCAAAAACC
ACACCCTGCCATTCACCCAA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MNIPNIISALTIAGSDTGGGAGIQADLKTFAAHHIHGLSAITVLTAQHTRGVNAVHIPPLEFIATQIDTCFSDFNVHAVK
LGMLANAKVITLVAEALQRHQPPCIVLDPVMIATSGSTLLEDSALEALRTQLLPLATLITPNIPEAERLLNRHINSGSDA
DTAAASLLKLGAHAVLLKGGHLNEGNSVIDRFDNGTLRERFSNPRLALNAHGTGCTLSSAITAQLCLGLNLHQACKAGID
YVARGLHAGYRPGRGNITVLNHIPPKHTE

Sequences:

>Translated_269_residues
MNIPNIISALTIAGSDTGGGAGIQADLKTFAAHHIHGLSAITVLTAQHTRGVNAVHIPPLEFIATQIDTCFSDFNVHAVK
LGMLANAKVITLVAEALQRHQPPCIVLDPVMIATSGSTLLEDSALEALRTQLLPLATLITPNIPEAERLLNRHINSGSDA
DTAAASLLKLGAHAVLLKGGHLNEGNSVIDRFDNGTLRERFSNPRLALNAHGTGCTLSSAITAQLCLGLNLHQACKAGID
YVARGLHAGYRPGRGNITVLNHIPPKHTE
>Mature_269_residues
MNIPNIISALTIAGSDTGGGAGIQADLKTFAAHHIHGLSAITVLTAQHTRGVNAVHIPPLEFIATQIDTCFSDFNVHAVK
LGMLANAKVITLVAEALQRHQPPCIVLDPVMIATSGSTLLEDSALEALRTQLLPLATLITPNIPEAERLLNRHINSGSDA
DTAAASLLKLGAHAVLLKGGHLNEGNSVIDRFDNGTLRERFSNPRLALNAHGTGCTLSSAITAQLCLGLNLHQACKAGID
YVARGLHAGYRPGRGNITVLNHIPPKHTE

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=219, Percent_Identity=49.7716894977169, Blast_Score=194, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6324997, Length=256, Percent_Identity=35.546875, Blast_Score=124, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325378, Length=272, Percent_Identity=31.25, Blast_Score=123, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6324517, Length=254, Percent_Identity=34.6456692913386, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28317; Mature: 28317

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIPNIISALTIAGSDTGGGAGIQADLKTFAAHHIHGLSAITVLTAQHTRGVNAVHIPPL
CCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHEEEEHHHCCCCCEEECCHH
EFIATQIDTCFSDFNVHAVKLGMLANAKVITLVAEALQRHQPPCIVLDPVMIATSGSTLL
HHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHCCCCEEEECCEEEEECCCHHH
EDSALEALRTQLLPLATLITPNIPEAERLLNRHINSGSDADTAAASLLKLGAHAVLLKGG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECC
HLNEGNSVIDRFDNGTLRERFSNPRLALNAHGTGCTLSSAITAQLCLGLNLHQACKAGID
CCCCCCHHHHHCCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
YVARGLHAGYRPGRGNITVLNHIPPKHTE
HHHHHHHCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MNIPNIISALTIAGSDTGGGAGIQADLKTFAAHHIHGLSAITVLTAQHTRGVNAVHIPPL
CCCCHHHHHHEEECCCCCCCCCHHHHHHHHHHHHHCCHHHHEEEEHHHCCCCCEEECCHH
EFIATQIDTCFSDFNVHAVKLGMLANAKVITLVAEALQRHQPPCIVLDPVMIATSGSTLL
HHHHHHHHHHHHCCCEEEEEEEHHCCCHHHHHHHHHHHHCCCCEEEECCEEEEECCCHHH
EDSALEALRTQLLPLATLITPNIPEAERLLNRHINSGSDADTAAASLLKLGAHAVLLKGG
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECC
HLNEGNSVIDRFDNGTLRERFSNPRLALNAHGTGCTLSSAITAQLCLGLNLHQACKAGID
CCCCCCHHHHHCCCCHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
YVARGLHAGYRPGRGNITVLNHIPPKHTE
HHHHHHHCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]