Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 182681419

GI number: 182681419

Start: 1021812

End: 1022561

Strand: Direct

Name: rlmB [H]

Synonym: XfasM23_0869

Alternate gene names: 182681419

Gene position: 1021812-1022561 (Clockwise)

Preceding gene: 182681418

Following gene: 182681422

Centisome position: 40.3

GC content: 54.53

Gene sequence:

>750_bases
ATGAATAAGAAAAACCAATGGATTGTTGGCATCAACGCCGTCGTGTCTTCTATTGAGAATGATGCTGAGCACGTGCGTGA
GGTGCTGGTTGAGGCTGCCAGTAAGAATTCACGGTTGCTTGATATTGAGGAAAATGCGCGGCGCAAGGGAATTGAGGTGC
GCCGGGTGACTACGCAAGCGTTGGATGGGGTTGGAGGTGGGGTACGCCATCAGGGGGTGGCCGCGCGTTATGCGGCTGTT
CGTCTCTGGGAGGAGCATGATCTAAAAGATTTAGTTGATGCGGCTGGAGGTCAAGCATTGTTGCTGGTGCTGGATGGAGT
ACAGGATCCTCATAATCTTGGGGCTTGTCTGCGTAGTGCCGCAGCGGCTGGGGTGACGGCGGTGATCATCCCCAAAGATA
AGTCTGTTGGTATCAATGCGACGGTACGCAAAACGTCGTCTGGTGCGGCGGATCGTCTTCCAGTCATTGCGGTGGTCAAT
CTGGCACGTTCCTTGCGCGAATTGCAGAAGCAGGATGTATGGATCTATGGGCTTGCGGGCGAGGTGGAGACTTCGTTGTA
TGCATTGGATCTGCGTGGCAACGTGGCGTTGGTGTTGGGTGGCGAGGCGGACGGCTTACGTCGTTTGACGCGTGAGCACT
GTGATGTGCTTGCACGGATTCCAATGCCAGGGGAGGTCGAAAGTTTGAATGTCTCGGTGGCAGCAGGGGTCACGTTGTTT
GAGGCAGTCCGCCAACGTACTTTAGTTTAA

Upstream 100 bases:

>100_bases
GATGGGCAGTAAAGGCCGTTAGATGCGCGCTGTTGTTGGTCCTAGTGGAGATCAATGCTTACGATAGTTCGTTTTTTTCA
GCTCTGGATCTCATTACGCT

Downstream 100 bases:

>100_bases
GGGTTTTGAAAGTTTGTTGAGTGTTTGAGATGCACGTTTCGCGCAAATGTCGCGGTATTTGGGATCTTCAAATGTCATTG
AGCTGGATAGGTAATTACTG

Product: RNA methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MNKKNQWIVGINAVVSSIENDAEHVREVLVEAASKNSRLLDIEENARRKGIEVRRVTTQALDGVGGGVRHQGVAARYAAV
RLWEEHDLKDLVDAAGGQALLLVLDGVQDPHNLGACLRSAAAAGVTAVIIPKDKSVGINATVRKTSSGAADRLPVIAVVN
LARSLRELQKQDVWIYGLAGEVETSLYALDLRGNVALVLGGEADGLRRLTREHCDVLARIPMPGEVESLNVSVAAGVTLF
EAVRQRTLV

Sequences:

>Translated_249_residues
MNKKNQWIVGINAVVSSIENDAEHVREVLVEAASKNSRLLDIEENARRKGIEVRRVTTQALDGVGGGVRHQGVAARYAAV
RLWEEHDLKDLVDAAGGQALLLVLDGVQDPHNLGACLRSAAAAGVTAVIIPKDKSVGINATVRKTSSGAADRLPVIAVVN
LARSLRELQKQDVWIYGLAGEVETSLYALDLRGNVALVLGGEADGLRRLTREHCDVLARIPMPGEVESLNVSVAAGVTLF
EAVRQRTLV
>Mature_249_residues
MNKKNQWIVGINAVVSSIENDAEHVREVLVEAASKNSRLLDIEENARRKGIEVRRVTTQALDGVGGGVRHQGVAARYAAV
RLWEEHDLKDLVDAAGGQALLLVLDGVQDPHNLGACLRSAAAAGVTAVIIPKDKSVGINATVRKTSSGAADRLPVIAVVN
LARSLRELQKQDVWIYGLAGEVETSLYALDLRGNVALVLGGEADGLRRLTREHCDVLARIPMPGEVESLNVSVAAGVTLF
EAVRQRTLV

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=259, Percent_Identity=30.1158301158301, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=50.2057613168724, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI1788935, Length=158, Percent_Identity=31.6455696202532, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324775, Length=160, Percent_Identity=31.875, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26656; Mature: 26656

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKNQWIVGINAVVSSIENDAEHVREVLVEAASKNSRLLDIEENARRKGIEVRRVTTQA
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHH
LDGVGGGVRHQGVAARYAAVRLWEEHDLKDLVDAAGGQALLLVLDGVQDPHNLGACLRSA
HHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
AAAGVTAVIIPKDKSVGINATVRKTSSGAADRLPVIAVVNLARSLRELQKQDVWIYGLAG
HHCCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECC
EVETSLYALDLRGNVALVLGGEADGLRRLTREHCDVLARIPMPGEVESLNVSVAAGVTLF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEHHHHHHHH
EAVRQRTLV
HHHHHHCCC
>Mature Secondary Structure
MNKKNQWIVGINAVVSSIENDAEHVREVLVEAASKNSRLLDIEENARRKGIEVRRVTTQA
CCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCHHHHHHHH
LDGVGGGVRHQGVAARYAAVRLWEEHDLKDLVDAAGGQALLLVLDGVQDPHNLGACLRSA
HHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHH
AAAGVTAVIIPKDKSVGINATVRKTSSGAADRLPVIAVVNLARSLRELQKQDVWIYGLAG
HHCCEEEEEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEEECC
EVETSLYALDLRGNVALVLGGEADGLRRLTREHCDVLARIPMPGEVESLNVSVAAGVTLF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHEEECCCCCCCCEEEEEHHHHHHHH
EAVRQRTLV
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]