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The map label for this gene is ilvE [H]

Identifier: 182681408

GI number: 182681408

Start: 1007517

End: 1008605

Strand: Direct

Name: ilvE [H]

Synonym: XfasM23_0857

Alternate gene names: 182681408

Gene position: 1007517-1008605 (Clockwise)

Preceding gene: 182681407

Following gene: 182681411

Centisome position: 39.73

GC content: 54.91

Gene sequence:

>1089_bases
GTGTCCATTTCGTTCGATATTGCCCGCTCATTGTCTACCACTTCTGATGAAGAGCTCTCTATGATCCTGCAAGCGCCAGG
GTTCGGTGTTCATTTCACTGATCATATGATTGCTATCGCCTGGGATAAGGACAATGATTGGCACGACGCCCAGGTGCGCG
CCTATGGGCCATTGTTGCTAGATCCTGCCGCTGCTGTGCTGCACTACGGGCAAGAGATTTTCGAAGGAATCAAAGCATAT
CGCCATGCTGATGGTTCGATCTGGACGTTCCGCCCGGAGGCCAACGCTCGCCGCTTGCAGCGTTCGGCCAAACGATTAGT
GCTGCCAGAGTTGCCGGTGGAACTGTTTACAGAATCTTTGCGTCAACTCATTGCAGTGGATGCCCGCTGGGTTCCTTCAG
CACCAGAAACCAGCCTTTATTTTCGTCCCTTCATGATTGCCACAGAAGCGTACTTAGGCGTGCGTGCTGCGCAGCACGCC
TCTTACTACCTGATCGCCAGCCCTGCTGGCCCGTATTTCGCTAAAGGCGTTACTCCCGTGTCGATCTGGTTGTCGACAGA
CTGTGCCCGTGCTGCCCTAGGGGGCACGGGCGCGGTGAAGTGTGGTGGCAATTATGCCGCCTCCTTGCTGCCGCAACAGG
TCGCGCAGACCCAGGGATGCTCTCAGGTACTGTTCCTTGATCCGGTTGAGGGCAAATACCTGGAGGAGCTGGGAGGGATG
AATGTGTTTCTCGTCTACACAGACGGCACCTTGGTCACACCAGCGTTGTCTGGCAGCATCCTCGAAGGTATCACCCGCGA
GAGTATCTTGCAGCTTGCTCGTGATCGAGGGATGTGTGTTGAGGAACGTAAGGTAGCAATTGAGGAGTGGAAAGAGGGCG
TGGTTTCCGGTGCGATCAGCGAGGTATTCGCTTGCGGTACGGCTGCTGTGATCACTCCAATTGGTGAATTGAAAGCGGAG
GGTTTTTCGGTCGGAAACATCAACGCACCACCTGGCGAAGTTACTCTGTCGTTGCGCAAGGAACTGACCGACATCCAGTA
CGGCCGTTTGCCAGACCGTTATGGGTGGATGACGTGTTTGAAGGAATAA

Upstream 100 bases:

>100_bases
GTTTTGAAATCTAGATTTCCCTGATCCGTTGCTGCCTGCTCCGATCCGTTGCTGCCTGCTCCTTCTGCCCTTTTGCTTCT
GCTTCAACAGGAATTTTCTA

Downstream 100 bases:

>100_bases
ATTACTTTCGCTTCCTATCCTTTTCGGTGACTTGATGCCGTTGGCTGGCTGCAATGCGGGCAATGCATGGGGGCTCTGCT
GCTGCTCCCGTCTTTGTTCG

Product: branched-chain amino acid aminotransferase

Products: NA

Alternate protein names: BCAT [H]

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSISFDIARSLSTTSDEELSMILQAPGFGVHFTDHMIAIAWDKDNDWHDAQVRAYGPLLLDPAAAVLHYGQEIFEGIKAY
RHADGSIWTFRPEANARRLQRSAKRLVLPELPVELFTESLRQLIAVDARWVPSAPETSLYFRPFMIATEAYLGVRAAQHA
SYYLIASPAGPYFAKGVTPVSIWLSTDCARAALGGTGAVKCGGNYAASLLPQQVAQTQGCSQVLFLDPVEGKYLEELGGM
NVFLVYTDGTLVTPALSGSILEGITRESILQLARDRGMCVEERKVAIEEWKEGVVSGAISEVFACGTAAVITPIGELKAE
GFSVGNINAPPGEVTLSLRKELTDIQYGRLPDRYGWMTCLKE

Sequences:

>Translated_362_residues
MSISFDIARSLSTTSDEELSMILQAPGFGVHFTDHMIAIAWDKDNDWHDAQVRAYGPLLLDPAAAVLHYGQEIFEGIKAY
RHADGSIWTFRPEANARRLQRSAKRLVLPELPVELFTESLRQLIAVDARWVPSAPETSLYFRPFMIATEAYLGVRAAQHA
SYYLIASPAGPYFAKGVTPVSIWLSTDCARAALGGTGAVKCGGNYAASLLPQQVAQTQGCSQVLFLDPVEGKYLEELGGM
NVFLVYTDGTLVTPALSGSILEGITRESILQLARDRGMCVEERKVAIEEWKEGVVSGAISEVFACGTAAVITPIGELKAE
GFSVGNINAPPGEVTLSLRKELTDIQYGRLPDRYGWMTCLKE
>Mature_361_residues
SISFDIARSLSTTSDEELSMILQAPGFGVHFTDHMIAIAWDKDNDWHDAQVRAYGPLLLDPAAAVLHYGQEIFEGIKAYR
HADGSIWTFRPEANARRLQRSAKRLVLPELPVELFTESLRQLIAVDARWVPSAPETSLYFRPFMIATEAYLGVRAAQHAS
YYLIASPAGPYFAKGVTPVSIWLSTDCARAALGGTGAVKCGGNYAASLLPQQVAQTQGCSQVLFLDPVEGKYLEELGGMN
VFLVYTDGTLVTPALSGSILEGITRESILQLARDRGMCVEERKVAIEEWKEGVVSGAISEVFACGTAAVITPIGELKAEG
FSVGNINAPPGEVTLSLRKELTDIQYGRLPDRYGWMTCLKE

Specific function: Catalyzes the reversible transfers of an amino group from glutamate to the alpha-ketoacid of the respective amino acid in the final step in the biosynthesis of branchedchain amino acids. The amino acids can be ranked in the following order with respect to

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI296010904, Length=330, Percent_Identity=40.3030303030303, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI296010906, Length=330, Percent_Identity=40.3030303030303, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI38176287, Length=330, Percent_Identity=40.3030303030303, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI50658084, Length=329, Percent_Identity=40.4255319148936, Blast_Score=225, Evalue=5e-59,
Organism=Homo sapiens, GI258614015, Length=285, Percent_Identity=40.7017543859649, Blast_Score=188, Evalue=5e-48,
Organism=Homo sapiens, GI296010902, Length=330, Percent_Identity=35.1515151515151, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI296010900, Length=330, Percent_Identity=35.1515151515151, Blast_Score=181, Evalue=6e-46,
Organism=Escherichia coli, GI48994963, Length=325, Percent_Identity=31.3846153846154, Blast_Score=112, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17568601, Length=344, Percent_Identity=38.0813953488372, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17565728, Length=342, Percent_Identity=35.9649122807018, Blast_Score=192, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6322002, Length=338, Percent_Identity=41.1242603550296, Blast_Score=229, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322608, Length=334, Percent_Identity=40.4191616766467, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI24641779, Length=347, Percent_Identity=39.193083573487, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005786 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.42 [H]

Molecular weight: Translated: 39480; Mature: 39349

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSISFDIARSLSTTSDEELSMILQAPGFGVHFTDHMIAIAWDKDNDWHDAQVRAYGPLLL
CCCCHHHHHHCCCCCHHHHHHHEECCCCCEEECCCEEEEEECCCCCCCCCEEEEECCEEE
DPAAAVLHYGQEIFEGIKAYRHADGSIWTFRPEANARRLQRSAKRLVLPELPVELFTESL
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
RQLIAVDARWVPSAPETSLYFRPFMIATEAYLGVRAAQHASYYLIASPAGPYFAKGVTPV
HHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCEE
SIWLSTDCARAALGGTGAVKCGGNYAASLLPQQVAQTQGCSQVLFLDPVEGKYLEELGGM
EEEEECCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCC
NVFLVYTDGTLVTPALSGSILEGITRESILQLARDRGMCVEERKVAIEEWKEGVVSGAIS
EEEEEEECCEEECCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EVFACGTAAVITPIGELKAEGFSVGNINAPPGEVTLSLRKELTDIQYGRLPDRYGWMTCL
HHHHCCCHHHHCCHHHHHCCCEEECCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHH
KE
CC
>Mature Secondary Structure 
SISFDIARSLSTTSDEELSMILQAPGFGVHFTDHMIAIAWDKDNDWHDAQVRAYGPLLL
CCCHHHHHHCCCCCHHHHHHHEECCCCCEEECCCEEEEEECCCCCCCCCEEEEECCEEE
DPAAAVLHYGQEIFEGIKAYRHADGSIWTFRPEANARRLQRSAKRLVLPELPVELFTESL
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
RQLIAVDARWVPSAPETSLYFRPFMIATEAYLGVRAAQHASYYLIASPAGPYFAKGVTPV
HHHHHHCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHCCCCEE
SIWLSTDCARAALGGTGAVKCGGNYAASLLPQQVAQTQGCSQVLFLDPVEGKYLEELGGM
EEEEECCHHHHHCCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHCCC
NVFLVYTDGTLVTPALSGSILEGITRESILQLARDRGMCVEERKVAIEEWKEGVVSGAIS
EEEEEEECCEEECCHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
EVFACGTAAVITPIGELKAEGFSVGNINAPPGEVTLSLRKELTDIQYGRLPDRYGWMTCL
HHHHCCCHHHHCCHHHHHCCCEEECCCCCCCCCEEHHHHHHHHHCCCCCCCCHHHHHHHH
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA