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The map label for this gene is dapF

Identifier: 182681289

GI number: 182681289

Start: 864330

End: 865184

Strand: Direct

Name: dapF

Synonym: XfasM23_0734

Alternate gene names: 182681289

Gene position: 864330-865184 (Clockwise)

Preceding gene: 182681288

Following gene: 182681290

Centisome position: 34.09

GC content: 55.67

Gene sequence:

>855_bases
ATGGGGGTTGAGAGCGTGCGATGTCCTTTGCATTTCACCAAGATGCAAGGGGCTGGTAATGACTTCGTCGTTTTGGATTT
GCGTGATGGGACACCGCCGCCGGATGCGGCGTTGGTCGCTTGGTTGGCTGATCGCCACTTTGGTATTGGGTGTGATCAGG
TTATCGCTATTGAGCCGCCGCGTGGTGTCGGGGTGTTTGCCGCTTACCGTATCTGGAATGCGGATGGGTCTGCGGCGCAG
CAATGTGGTAATGGTGCACGTTGCGTCGCTGCTTGGTTGGTGCGTGATGGTTCCGTTGCGACCGAGCATTTCTTGATTGA
CAGTCCGGTACAGACGCATTCGGTTCGCTGCATAGGTAAAGATGAGTATGCGGTGGAGATGGGTCTGCCAGTGTTCGAGC
CTGAGCGTATTCCTTTGTCTGGATTTCCCAATGCCTTGGGAGAGTATGTGTTGTCGCTACAGGGTGAGGTGCTGTGTTGT
GGTGCGGTATCAATGGGGAATCCGCATGCAGTGGTTGAGGTCGATTTGATTGATGTGGCACCTGTCGAACGGATCGGGCC
GCTCTTGCAGCAGCATTCTGCGTTTCCGGAGTCAGTGAATGTCAGTTTTGTCCAGGTGATTGATCCTGGTTTGGTACGGT
TGCGTGTGTATGAGCGTGGTGCTGGAGAAACGTTGGCGTGTGGCAGTGGTGCTTGTGCGGCAGCAGTTGTGCTGATGCAG
CGTGGCAGGGTTGGACGTGATGTGCGTGTTGTTCTACCAGGGGGCACGTTGCGTGTGCAGTGGCCAGTTTCCGGTGGGCC
AGTGACGTTGTCTGGTCCGGCAAGGTGTGTGTTTGATGGAGTGTGGTACGGATGA

Upstream 100 bases:

>100_bases
AGAGCAGCCGTTGTCGTTGCCTAAGGCTAAATCTCAGGGGGATAAGGGAGCTAGCCCGCCGCCGACTGCAAAGCCTGTTC
GGTCCCGGTGATGCAAGACG

Downstream 100 bases:

>100_bases
GTGAGGTGAAGGATAAGATCTGTGCATACGACGTGGCTGCTTGGCTGCGTCGGCATCCCGCTTTTCTTAAGCAGTTCCCT
GATTTGGCGTTGACGTTGGT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MGVESVRCPLHFTKMQGAGNDFVVLDLRDGTPPPDAALVAWLADRHFGIGCDQVIAIEPPRGVGVFAAYRIWNADGSAAQ
QCGNGARCVAAWLVRDGSVATEHFLIDSPVQTHSVRCIGKDEYAVEMGLPVFEPERIPLSGFPNALGEYVLSLQGEVLCC
GAVSMGNPHAVVEVDLIDVAPVERIGPLLQQHSAFPESVNVSFVQVIDPGLVRLRVYERGAGETLACGSGACAAAVVLMQ
RGRVGRDVRVVLPGGTLRVQWPVSGGPVTLSGPARCVFDGVWYG

Sequences:

>Translated_284_residues
MGVESVRCPLHFTKMQGAGNDFVVLDLRDGTPPPDAALVAWLADRHFGIGCDQVIAIEPPRGVGVFAAYRIWNADGSAAQ
QCGNGARCVAAWLVRDGSVATEHFLIDSPVQTHSVRCIGKDEYAVEMGLPVFEPERIPLSGFPNALGEYVLSLQGEVLCC
GAVSMGNPHAVVEVDLIDVAPVERIGPLLQQHSAFPESVNVSFVQVIDPGLVRLRVYERGAGETLACGSGACAAAVVLMQ
RGRVGRDVRVVLPGGTLRVQWPVSGGPVTLSGPARCVFDGVWYG
>Mature_283_residues
GVESVRCPLHFTKMQGAGNDFVVLDLRDGTPPPDAALVAWLADRHFGIGCDQVIAIEPPRGVGVFAAYRIWNADGSAAQQ
CGNGARCVAAWLVRDGSVATEHFLIDSPVQTHSVRCIGKDEYAVEMGLPVFEPERIPLSGFPNALGEYVLSLQGEVLCCG
AVSMGNPHAVVEVDLIDVAPVERIGPLLQQHSAFPESVNVSFVQVIDPGLVRLRVYERGAGETLACGSGACAAAVVLMQR
GRVGRDVRVVLPGGTLRVQWPVSGGPVTLSGPARCVFDGVWYG

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=43.7956204379562, Blast_Score=233, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_XYLF2 (B2IA16)

Other databases:

- EMBL:   CP001011
- RefSeq:   YP_001829449.1
- ProteinModelPortal:   B2IA16
- SMR:   B2IA16
- GeneID:   6202917
- GenomeReviews:   CP001011_GR
- KEGG:   xfn:XfasM23_0734
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30098; Mature: 29967

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 82-82 ACT_SITE 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVESVRCPLHFTKMQGAGNDFVVLDLRDGTPPPDAALVAWLADRHFGIGCDQVIAIEPP
CCCCCEECCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCC
RGVGVFAAYRIWNADGSAAQQCGNGARCVAAWLVRDGSVATEHFLIDSPVQTHSVRCIGK
CCCCEEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCEEEEECC
DEYAVEMGLPVFEPERIPLSGFPNALGEYVLSLQGEVLCCGAVSMGNPHAVVEVDLIDVA
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCC
PVERIGPLLQQHSAFPESVNVSFVQVIDPGLVRLRVYERGAGETLACGSGACAAAVVLMQ
CHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEEECCCCCEEECCCCHHHHHHHHHH
RGRVGRDVRVVLPGGTLRVQWPVSGGPVTLSGPARCVFDGVWYG
CCCCCCCEEEEECCCEEEEEEECCCCCEEECCCCEEEECCCCCC
>Mature Secondary Structure 
GVESVRCPLHFTKMQGAGNDFVVLDLRDGTPPPDAALVAWLADRHFGIGCDQVIAIEPP
CCCCEECCEEEEEECCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCC
RGVGVFAAYRIWNADGSAAQQCGNGARCVAAWLVRDGSVATEHFLIDSPVQTHSVRCIGK
CCCCEEEEEEEECCCCHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCEEEEECC
DEYAVEMGLPVFEPERIPLSGFPNALGEYVLSLQGEVLCCGAVSMGNPHAVVEVDLIDVA
CCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCEEEEEEEECCC
PVERIGPLLQQHSAFPESVNVSFVQVIDPGLVRLRVYERGAGETLACGSGACAAAVVLMQ
CHHHHHHHHHHHCCCCCCCCEEEEEEECCCEEEEEEEECCCCCEEECCCCHHHHHHHHHH
RGRVGRDVRVVLPGGTLRVQWPVSGGPVTLSGPARCVFDGVWYG
CCCCCCCEEEEECCCEEEEEEECCCCCEEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA