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The map label for this gene is tufB [H]

Identifier: 197294694

GI number: 197294694

Start: 656879

End: 658063

Strand: Reverse

Name: tufB [H]

Synonym: PAa_0660

Alternate gene names: 197294694

Gene position: 658063-656879 (Counterclockwise)

Preceding gene: 197294695

Following gene: 197294684

Centisome position: 74.78

GC content: 36.46

Gene sequence:

>1185_bases
ATGGCTAATGAAAAATTTATAAGAAATAAACCTCATTTAAATGTTGGAACTATTGGACACGTAGACCACGGAAAAACTAC
TTTAACAGCTGCAATAACTCAAGTTTTATCTACTCAAGGATTGGCTAAAAGTAAAGCTTATGACCAAATTGATAACGCTC
CTGAAGAAAGAGAACGTGGAATCACCATCAAAACTTCTCACGTTGAATATGAAACTTCTAAAAGACATTACGCTCACGTT
GACTGCCCTGGGCACGCTGACTATGTTAAAAACATGATTACTGGAGCCGCTCAAATGGACGCTGCCATTCTAGTTGTTTC
TGGTGCAGATAGTGTAATGCCTCAAACTAGAGAACATATTTTATTGGCTCGCCAAGTTGGAGTTCCTAAAATTGTTGTTT
TCTTAAATAAATGTGATCTTTGCCCTGATGAAGAAATTTTAGAATTAGTGGAAATGGAAGTTCGTGAATTATTATCTAAA
TACGATTTTCCGGGTGATGACACACCAATTATCAGAGGATCTGCTTTAAAAGCTTTAGAAGGTGACGCTCATTACGTTGC
TCAAGTTAATGAATTAATTCAAACTTTAGATACTTATATTGAAGACCCAGCTCGTGAAGTTGACAAACCTTTCTTAATGC
CAGTTGAAGATGTTTTCACTATTACTGGTAGAGGAACAGTTGTTACTGGTAGAGTTGAAAGAGGTCAAGTAAAAGCTGGT
GATGAGATTGAAATCGTAGGTTTGAAAGAAACTAAAAAAACAATTGTAACAGCAGTGGAAATGTTTAAAAAAGACCTAGA
TTTTGCTCAAGCAGGTGATAACATTGGTGCTTTATTACGTGGGATTAACCGAGAAGACGTTCAACGTGGACAAGTTTTGG
CTAAACCAGGATCTGTTAAACCTCATTCTAAATTCGTTGCTCAAGTTTATGTTTTGACCAAAGAAGAAGGCGGCCGTCAT
ACTGCATTTTTCTCCCAATATCGTCCTCAATTTTATTTCCGTACTACTGATATTACAGGGGTGGTTGAATTACAAGGTGA
TGTTAAAATGGTTATGCCAGGAGACAACACTGAATTAGTTGTTACTTTAAACAACCCTATTGCAATTGAAGAAGGAACTA
AATTTTCTATTCGTGAAGGCGGAAAAACAGTTGGCGCTGGATCAGTTTCTAAGATTTTAAAATAA

Upstream 100 bases:

>100_bases
TAATATAAAACAGTTGATATATTTTTAAAAATAAGTTAAAATTTAAATGGTAATAATTATATTAAAAACCTAAACGAAAC
AAAAGGAGGCCTTTTGCAAA

Downstream 100 bases:

>100_bases
TTTTTTTGAAAAAAGCCTTAAAAAAGGCTTTTTTTCATTTTTTAGAAATTATTATTAATAAAAAAAATAAAAAAAGGTAA
TATATAAGTGCACTTTAAGA

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MANEKFIRNKPHLNVGTIGHVDHGKTTLTAAITQVLSTQGLAKSKAYDQIDNAPEERERGITIKTSHVEYETSKRHYAHV
DCPGHADYVKNMITGAAQMDAAILVVSGADSVMPQTREHILLARQVGVPKIVVFLNKCDLCPDEEILELVEMEVRELLSK
YDFPGDDTPIIRGSALKALEGDAHYVAQVNELIQTLDTYIEDPAREVDKPFLMPVEDVFTITGRGTVVTGRVERGQVKAG
DEIEIVGLKETKKTIVTAVEMFKKDLDFAQAGDNIGALLRGINREDVQRGQVLAKPGSVKPHSKFVAQVYVLTKEEGGRH
TAFFSQYRPQFYFRTTDITGVVELQGDVKMVMPGDNTELVVTLNNPIAIEEGTKFSIREGGKTVGAGSVSKILK

Sequences:

>Translated_394_residues
MANEKFIRNKPHLNVGTIGHVDHGKTTLTAAITQVLSTQGLAKSKAYDQIDNAPEERERGITIKTSHVEYETSKRHYAHV
DCPGHADYVKNMITGAAQMDAAILVVSGADSVMPQTREHILLARQVGVPKIVVFLNKCDLCPDEEILELVEMEVRELLSK
YDFPGDDTPIIRGSALKALEGDAHYVAQVNELIQTLDTYIEDPAREVDKPFLMPVEDVFTITGRGTVVTGRVERGQVKAG
DEIEIVGLKETKKTIVTAVEMFKKDLDFAQAGDNIGALLRGINREDVQRGQVLAKPGSVKPHSKFVAQVYVLTKEEGGRH
TAFFSQYRPQFYFRTTDITGVVELQGDVKMVMPGDNTELVVTLNNPIAIEEGTKFSIREGGKTVGAGSVSKILK
>Mature_393_residues
ANEKFIRNKPHLNVGTIGHVDHGKTTLTAAITQVLSTQGLAKSKAYDQIDNAPEERERGITIKTSHVEYETSKRHYAHVD
CPGHADYVKNMITGAAQMDAAILVVSGADSVMPQTREHILLARQVGVPKIVVFLNKCDLCPDEEILELVEMEVRELLSKY
DFPGDDTPIIRGSALKALEGDAHYVAQVNELIQTLDTYIEDPAREVDKPFLMPVEDVFTITGRGTVVTGRVERGQVKAGD
EIEIVGLKETKKTIVTAVEMFKKDLDFAQAGDNIGALLRGINREDVQRGQVLAKPGSVKPHSKFVAQVYVLTKEEGGRHT
AFFSQYRPQFYFRTTDITGVVELQGDVKMVMPGDNTELVVTLNNPIAIEEGTKFSIREGGKTVGAGSVSKILK

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=55.3299492385787, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI4503475, Length=453, Percent_Identity=29.3598233995585, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4503471, Length=453, Percent_Identity=29.5805739514349, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI223555963, Length=441, Percent_Identity=28.3446712018141, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI5729864, Length=441, Percent_Identity=28.3446712018141, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI54607086, Length=315, Percent_Identity=31.1111111111111, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI194018522, Length=340, Percent_Identity=29.1176470588235, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI194018520, Length=340, Percent_Identity=29.1176470588235, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI194097354, Length=340, Percent_Identity=29.1176470588235, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI46094014, Length=324, Percent_Identity=28.7037037037037, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI4503507, Length=454, Percent_Identity=26.431718061674, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157426893, Length=235, Percent_Identity=27.2340425531915, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790412, Length=393, Percent_Identity=71.2468193384224, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789737, Length=393, Percent_Identity=71.2468193384224, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI2367247, Length=370, Percent_Identity=28.3783783783784, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=30.1818181818182, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789108, Length=331, Percent_Identity=31.4199395770393, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI1788922, Length=327, Percent_Identity=24.7706422018349, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=30.1369863013699, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI17556456, Length=396, Percent_Identity=52.020202020202, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=385, Percent_Identity=43.6363636363636, Blast_Score=333, Evalue=1e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=30.1762114537445, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=30.1762114537445, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=29.1284403669725, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17506081, Length=302, Percent_Identity=30.794701986755, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32566629, Length=339, Percent_Identity=30.0884955752212, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=441, Percent_Identity=26.530612244898, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=441, Percent_Identity=26.530612244898, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=38.255033557047, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=231, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=61.1111111111111, Blast_Score=498, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6325337, Length=447, Percent_Identity=29.3064876957494, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=447, Percent_Identity=29.3064876957494, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=351, Percent_Identity=26.7806267806268, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6320377, Length=343, Percent_Identity=26.530612244898, Blast_Score=98, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=344, Percent_Identity=29.6511627906977, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323320, Length=247, Percent_Identity=29.5546558704453, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281363316, Length=396, Percent_Identity=56.5656565656566, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI17864358, Length=396, Percent_Identity=56.5656565656566, Blast_Score=460, Evalue=1e-130,
Organism=Drosophila melanogaster, GI19921738, Length=389, Percent_Identity=48.0719794344473, Blast_Score=393, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=453, Percent_Identity=29.8013245033113, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553816, Length=453, Percent_Identity=29.8013245033113, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24651721, Length=453, Percent_Identity=29.8013245033113, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17864154, Length=453, Percent_Identity=29.8013245033113, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24652838, Length=353, Percent_Identity=32.8611898016997, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137572, Length=353, Percent_Identity=32.8611898016997, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922690, Length=303, Percent_Identity=31.3531353135314, Blast_Score=142, Evalue=4e-34,
Organism=Drosophila melanogaster, GI17137380, Length=345, Percent_Identity=29.8550724637681, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI45550900, Length=431, Percent_Identity=27.8422273781903, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647048, Length=354, Percent_Identity=27.9661016949153, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24647046, Length=354, Percent_Identity=27.9661016949153, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI78706572, Length=311, Percent_Identity=26.0450160771704, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI28572034, Length=240, Percent_Identity=32.0833333333333, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 43343; Mature: 43212

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANEKFIRNKPHLNVGTIGHVDHGKTTLTAAITQVLSTQGLAKSKAYDQIDNAPEERERG
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCC
ITIKTSHVEYETSKRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSGADSVMPQTREHI
CEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCHHHHH
LLARQVGVPKIVVFLNKCDLCPDEEILELVEMEVRELLSKYDFPGDDTPIIRGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHC
GDAHYVAQVNELIQTLDTYIEDPAREVDKPFLMPVEDVFTITGRGTVVTGRVERGQVKAG
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHEEEECCCEEEEECCCCCCCCCC
DEIEIVGLKETKKTIVTAVEMFKKDLDFAQAGDNIGALLRGINREDVQRGQVLAKPGSVK
CEEEEEECHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCCHHHHHCCCEEECCCCCC
PHSKFVAQVYVLTKEEGGRHTAFFSQYRPQFYFRTTDITGVVELQGDVKMVMPGDNTELV
CHHHEEEEEEEEEECCCCCEEHHHHHCCCEEEEEECCCEEEEEECCCEEEEECCCCCEEE
VTLNNPIAIEEGTKFSIREGGKTVGAGSVSKILK
EEECCCEEEECCCEEEECCCCCCCCCCHHHHHCC
>Mature Secondary Structure 
ANEKFIRNKPHLNVGTIGHVDHGKTTLTAAITQVLSTQGLAKSKAYDQIDNAPEERERG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHCC
ITIKTSHVEYETSKRHYAHVDCPGHADYVKNMITGAAQMDAAILVVSGADSVMPQTREHI
CEEEEECEEEECCCCEEEEECCCCHHHHHHHHHHCHHHCCEEEEEEECCCCCCCCHHHHH
LLARQVGVPKIVVFLNKCDLCPDEEILELVEMEVRELLSKYDFPGDDTPIIRGSALKALE
HHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCHHHHHC
GDAHYVAQVNELIQTLDTYIEDPAREVDKPFLMPVEDVFTITGRGTVVTGRVERGQVKAG
CCHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHHHEEEECCCEEEEECCCCCCCCCC
DEIEIVGLKETKKTIVTAVEMFKKDLDFAQAGDNIGALLRGINREDVQRGQVLAKPGSVK
CEEEEEECHHHHHHHHHHHHHHHHHCCHHHCCCCHHHHHHCCCHHHHHCCCEEECCCCCC
PHSKFVAQVYVLTKEEGGRHTAFFSQYRPQFYFRTTDITGVVELQGDVKMVMPGDNTELV
CHHHEEEEEEEEEECCCCCEEHHHHHCCCEEEEEECCCEEEEEECCCEEEEECCCCCEEE
VTLNNPIAIEEGTKFSIREGGKTVGAGSVSKILK
EEECCCEEEECCCEEEECCCCCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA