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The map label for this gene is dnaG [H]

Identifier: 197294658

GI number: 197294658

Start: 609757

End: 611505

Strand: Reverse

Name: dnaG [H]

Synonym: PAa_0614

Alternate gene names: 197294658

Gene position: 611505-609757 (Counterclockwise)

Preceding gene: 197294659

Following gene: 197294657

Centisome position: 69.49

GC content: 25.56

Gene sequence:

>1749_bases
ATGCAAAAACAATTAATTCAAGAAATTAATGAAAAAACATCCATTGTTGAATTAGCTCAAGAGTTTGTTCAACTAAAAAA
ATCTGGAAAGAATTATATGGGGTTATGCCCTTTTCACGATGAAAAAACCCCTTCTTTTTCGGTTTCTCCTGAAAAAAATA
TTGCAGTTTGCATGTCTTGCAAAAAAGGCGGCAACCCTGTTTTTTTTTATCAAAATATTAAAAATATTTCCTTTCGTGAA
GCAGTAGCTCAATTAGGAGCTCGCTTAGGAATCGCTTCTCTATCTTTTGATTCTAAGAAAGATAAACACGCTCATTTATA
TGAAATTATGAAAGAAGCGACTGAGTTTTTCCAAATTCAGTTAAAACATAATGAAAAAGCTTTGCAATATCTTAAAACAA
GGGGATTAGATGAAAAAATAATTACCCATTTTAAATTAGGATATGCTCCCAATTGGTCTTATTTATCGCGTTATTTAACT
GAAGAAACAAAATTTGAATATTGCGATTTAAAAGCGTTAAGTTTAGTTAATCAAAATGAACAAACTGGAAAATTTTATGA
TTTTTTTCAAAATAGATTAATTTTTCCAATTACTAATCTTAACGGTCAAATAATTGCTTTTTCTAGTCGTAGTTTAACCG
ATCAAATTCCTAAATATTTAAATAGTCCAGAAACAGTTCTTTTTAAGAAAGGTGAAGTTTTATATCAATATTTTGAAAAT
CAAGCCGAAATAAGAAAAAAAGAAACAATTATTTTGCATGAAGGTTTTTTTGATGTTATATCATCTTTTAAAGTTGGAGT
GAAAAATGTTGTTGCCACAATGGGAACCAATTTAACTCAAAATCATTTGAAGCTTTTAAGCAAGTTAGCACAAAAAATAA
TTATTGCTTATGATGGGGATGCATCAGGAAAAAAATCTGCTTTATCAATTGGAAACACTTTACAACAAAAGAATTTTGAT
GTTAAAATCCTTGATTTCCCTAATAATTTAGATCCTGATGAATACATTAAAACTGAGGGAGCGGAAAAATATCATCAATT
ATTAACTAATCATTTAACAGATTTTTTAACTTTACAAATTGATTTAATTTGCAAAAAAATTAGTTGGAATAATAGAAAAA
AAATTGAAAAAGAATTAAAAATGTTATTTCAAAACCAAGATTTAGCTACTAAAACGGTTTATCAATCCTATCTTGCTCAA
AAATACCAATTATTTATTGATTTCAACGTTCAACAACAAGAACTAATAATTCCAACCTTAATTTCTTCCCAAAATACCAT
CAATTATAAAAAATTTGATAAAGAAATTCATATTTTAATAGAATTTATTAATAATAGGTCTTTTTTTGAAAAAAATTATC
AAATAAATAATGATGCATTAGTTTATTCAGATGTTAACGTTTCTTATTTCATTGAAGAAATTAAAACTTATTATCAAAAT
AATTTACTTAAAAATTCCATCCCTTGGAATCATTTAGAAAAAAATGACAATGAATCCCTCCATCCATTAATAGAAGAAAT
CAAAAACCATCATTTTTTTCAAGCCAAAATTTCTTTAGATCAAAATACCTTTGAAAAATATATTCAAGATTTCAAAAATG
CCAAAACCATCCAAGAATTAAAAGAGGAAATCAACAAATTAAGAATTAACATAAAAATGCATGAAGACTTAAAAGAAAAA
ACTCAATATTTATATCAATTAAGTGATTTGCAGAAAAAACTAAAAAAACTTTTAGAAAAAATAAACTAA

Upstream 100 bases:

>100_bases
ACAGTCACTTTAAGAAATCGCGACACTTTAAAACAAGAAACAATGACTTTAGAAGAAGTTAAAAATTATGTTTTATCGCA
GGTGACATTAAAATAAAGTT

Downstream 100 bases:

>100_bases
TTTTGAGAGAAAAAATAAAAAATAAAGGTAGGTTAAAAATGGATTTTAAGGAAGCTATTAGAATTTTAAATAATAAAATA
GGCCAAAACAAAGAAATAAC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MQKQLIQEINEKTSIVELAQEFVQLKKSGKNYMGLCPFHDEKTPSFSVSPEKNIAVCMSCKKGGNPVFFYQNIKNISFRE
AVAQLGARLGIASLSFDSKKDKHAHLYEIMKEATEFFQIQLKHNEKALQYLKTRGLDEKIITHFKLGYAPNWSYLSRYLT
EETKFEYCDLKALSLVNQNEQTGKFYDFFQNRLIFPITNLNGQIIAFSSRSLTDQIPKYLNSPETVLFKKGEVLYQYFEN
QAEIRKKETIILHEGFFDVISSFKVGVKNVVATMGTNLTQNHLKLLSKLAQKIIIAYDGDASGKKSALSIGNTLQQKNFD
VKILDFPNNLDPDEYIKTEGAEKYHQLLTNHLTDFLTLQIDLICKKISWNNRKKIEKELKMLFQNQDLATKTVYQSYLAQ
KYQLFIDFNVQQQELIIPTLISSQNTINYKKFDKEIHILIEFINNRSFFEKNYQINNDALVYSDVNVSYFIEEIKTYYQN
NLLKNSIPWNHLEKNDNESLHPLIEEIKNHHFFQAKISLDQNTFEKYIQDFKNAKTIQELKEEINKLRINIKMHEDLKEK
TQYLYQLSDLQKKLKKLLEKIN

Sequences:

>Translated_582_residues
MQKQLIQEINEKTSIVELAQEFVQLKKSGKNYMGLCPFHDEKTPSFSVSPEKNIAVCMSCKKGGNPVFFYQNIKNISFRE
AVAQLGARLGIASLSFDSKKDKHAHLYEIMKEATEFFQIQLKHNEKALQYLKTRGLDEKIITHFKLGYAPNWSYLSRYLT
EETKFEYCDLKALSLVNQNEQTGKFYDFFQNRLIFPITNLNGQIIAFSSRSLTDQIPKYLNSPETVLFKKGEVLYQYFEN
QAEIRKKETIILHEGFFDVISSFKVGVKNVVATMGTNLTQNHLKLLSKLAQKIIIAYDGDASGKKSALSIGNTLQQKNFD
VKILDFPNNLDPDEYIKTEGAEKYHQLLTNHLTDFLTLQIDLICKKISWNNRKKIEKELKMLFQNQDLATKTVYQSYLAQ
KYQLFIDFNVQQQELIIPTLISSQNTINYKKFDKEIHILIEFINNRSFFEKNYQINNDALVYSDVNVSYFIEEIKTYYQN
NLLKNSIPWNHLEKNDNESLHPLIEEIKNHHFFQAKISLDQNTFEKYIQDFKNAKTIQELKEEINKLRINIKMHEDLKEK
TQYLYQLSDLQKKLKKLLEKIN
>Mature_582_residues
MQKQLIQEINEKTSIVELAQEFVQLKKSGKNYMGLCPFHDEKTPSFSVSPEKNIAVCMSCKKGGNPVFFYQNIKNISFRE
AVAQLGARLGIASLSFDSKKDKHAHLYEIMKEATEFFQIQLKHNEKALQYLKTRGLDEKIITHFKLGYAPNWSYLSRYLT
EETKFEYCDLKALSLVNQNEQTGKFYDFFQNRLIFPITNLNGQIIAFSSRSLTDQIPKYLNSPETVLFKKGEVLYQYFEN
QAEIRKKETIILHEGFFDVISSFKVGVKNVVATMGTNLTQNHLKLLSKLAQKIIIAYDGDASGKKSALSIGNTLQQKNFD
VKILDFPNNLDPDEYIKTEGAEKYHQLLTNHLTDFLTLQIDLICKKISWNNRKKIEKELKMLFQNQDLATKTVYQSYLAQ
KYQLFIDFNVQQQELIIPTLISSQNTINYKKFDKEIHILIEFINNRSFFEKNYQINNDALVYSDVNVSYFIEEIKTYYQN
NLLKNSIPWNHLEKNDNESLHPLIEEIKNHHFFQAKISLDQNTFEKYIQDFKNAKTIQELKEEINKLRINIKMHEDLKEK
TQYLYQLSDLQKKLKKLLEKIN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=380, Percent_Identity=33.4210526315789, Blast_Score=216, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF00772 DnaB; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 68236; Mature: 68236

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: PS00268 CECROPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKQLIQEINEKTSIVELAQEFVQLKKSGKNYMGLCPFHDEKTPSFSVSPEKNIAVCMSC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEE
KKGGNPVFFYQNIKNISFREAVAQLGARLGIASLSFDSKKDKHAHLYEIMKEATEFFQIQ
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHEEEE
LKHNEKALQYLKTRGLDEKIITHFKLGYAPNWSYLSRYLTEETKFEYCDLKALSLVNQNE
ECCCHHHHHHHHHCCCCHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
QTGKFYDFFQNRLIFPITNLNGQIIAFSSRSLTDQIPKYLNSPETVLFKKGEVLYQYFEN
CCCHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHH
QAEIRKKETIILHEGFFDVISSFKVGVKNVVATMGTNLTQNHLKLLSKLAQKIIIAYDGD
HHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCC
ASGKKSALSIGNTLQQKNFDVKILDFPNNLDPDEYIKTEGAEKYHQLLTNHLTDFLTLQI
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DLICKKISWNNRKKIEKELKMLFQNQDLATKTVYQSYLAQKYQLFIDFNVQQQELIIPTL
HHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCEEHHHH
ISSQNTINYKKFDKEIHILIEFINNRSFFEKNYQINNDALVYSDVNVSYFIEEIKTYYQN
HCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCEECCCEEEEECCCHHHHHHHHHHHHHH
NLLKNSIPWNHLEKNDNESLHPLIEEIKNHHFFQAKISLDQNTFEKYIQDFKNAKTIQEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCHHHHHHH
KEEINKLRINIKMHEDLKEKTQYLYQLSDLQKKLKKLLEKIN
HHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKQLIQEINEKTSIVELAQEFVQLKKSGKNYMGLCPFHDEKTPSFSVSPEKNIAVCMSC
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCEEEEEEE
KKGGNPVFFYQNIKNISFREAVAQLGARLGIASLSFDSKKDKHAHLYEIMKEATEFFQIQ
CCCCCEEEEECCCCCCHHHHHHHHHHHHHCEEECCCCCCCCCHHHHHHHHHHHHHHEEEE
LKHNEKALQYLKTRGLDEKIITHFKLGYAPNWSYLSRYLTEETKFEYCDLKALSLVNQNE
ECCCHHHHHHHHHCCCCHHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCC
QTGKFYDFFQNRLIFPITNLNGQIIAFSSRSLTDQIPKYLNSPETVLFKKGEVLYQYFEN
CCCHHHHHHHCCEEEEEECCCCEEEEECCCCHHHHHHHHHCCCCEEEEECHHHHHHHHHH
QAEIRKKETIILHEGFFDVISSFKVGVKNVVATMGTNLTQNHLKLLSKLAQKIIIAYDGD
HHHHHHHCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHEEEEECCC
ASGKKSALSIGNTLQQKNFDVKILDFPNNLDPDEYIKTEGAEKYHQLLTNHLTDFLTLQI
CCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
DLICKKISWNNRKKIEKELKMLFQNQDLATKTVYQSYLAQKYQLFIDFNVQQQELIIPTL
HHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCEEHHHH
ISSQNTINYKKFDKEIHILIEFINNRSFFEKNYQINNDALVYSDVNVSYFIEEIKTYYQN
HCCCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCEECCCEEEEECCCHHHHHHHHHHHHHH
NLLKNSIPWNHLEKNDNESLHPLIEEIKNHHFFQAKISLDQNTFEKYIQDFKNAKTIQEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHCHHHHHHH
KEEINKLRINIKMHEDLKEKTQYLYQLSDLQKKLKKLLEKIN
HHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA