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The map label for this gene is lysU

Identifier: 197294580

GI number: 197294580

Start: 534950

End: 536443

Strand: Reverse

Name: lysU

Synonym: PAa_0536

Alternate gene names: 197294580

Gene position: 536443-534950 (Counterclockwise)

Preceding gene: 197294588

Following gene: 197294579

Centisome position: 60.96

GC content: 30.05

Gene sequence:

>1494_bases
ATGAATAAAAAAATGACAGAACAAGAAGGAATTAGACACCAAAAAATAGAAGAATTGAAAAAATTAAAAACTGAACCTTT
TGGTAAAAATTTCACTTGTTCGCACACAATTGAAAACATTATTTTAAATTATCAAAACATTGATCAAGAATGGTTCGAAG
AAAACAAAGTTAATGTAAAAATTGCAGGAAGAATTATTTTAAAAAGAGGACAAGGAAAAGCCGGATTTTTACATCTACAA
GATTTTAATTTTAAAATTCAAGTTTATCTTAGATCTGATTTATTAGGGGATGCTTTCCTTCTTTATCAAAATTGTGATTT
AGGTGATATCGTAGGGATTAAAGGTTTTTTGTTTAAAACTAAAACAAAAGAGCTCACAATTAAAGCTCTAGATTTTATCC
ATTTAACTAAATCTTTAAAACCATTACCAGATAAATTTCATGGTTTGCAAAACCGCGAAGAGATGAGAAAAAAACGTTAT
TTAGATCTTATGGTTAATGAAAAAAGCCGACAAGTTTTTTTAACTCGTAGTTTAATTATCAAATCAATTCGTAATTTTTT
TGATAATGAGGGCTTTTTGGAGGTAGAAACCCCCATTTTGCAACCTTCTTTGGGCGGAGCTTCTGCCAAGCCATTTATTA
CTAGCCATAACGCTTTAAATTCTGATTTTTATTTAAGGATAGCTACCGAACTGCCTTTAAAAAAGTTAATTGTTGGTGGT
ATGCCAAAAGTTTATGAAATTGGTCGAATTTTCCGTAATGAAGGAATGGATGCAACCCATAACCCGGAATTTAGCACTAT
AGAGGCTTATCAGGCTTATTCTGATATACAAGGAATGATGGAATTAACTCAAAAATGTCTTCAGTATATTTGTCAAACTG
TTTTAAAAAGTTTAGAAATCAATTACCAAAATAAAAAAATTAGTTTTCAAACTTTTCAAAAAATTAGCATGACAGATGCT
ATCAAAAAAGAAACAGGCATTGATTTCCAAAAAAAATTATCTCAAGAAACTTGTTTGCAATTAGCAAAACAACATGATAT
CAACGTCATGTCTCATTTTAGCCAAGGTCATATTATTGAAGTTTTTTTTGAAAAATACGTTGAACATAAATTAATCCAGC
CTACTTTTATTTACGGCCATCCTTTAGAAATTAGTCCTTTAGCCAAAAAAAATCTTAATAACCCTCGTTTTACTGATCGT
TTTGAACTTTTTATCGCTGGAAAAGAATTTGTGAATGCTTTTAGCGAATTAAACGACCCCATCGAACAAGAAAAACGTTT
TTTAAATCAATTAAAACAAAAAGAATTAGGTGACGATGAGGCTTGTGAAATGGATTATGATTTTTTAGATGCCTTAAGTT
ACGGTATGCCTCCCACTGGTGGGCTCGGAATGGGAATTGATCGAATAGTGATGCTTTTAACCGATACTCCCAATATCCGT
GATGTTATTTTATTTCCTCATTTCAAAAATAAAAACATTATTAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TTAAGCATAATATATGGCAAAAAAACATTATTTTTTGGTATAATTATAATTATAATGTTTTGAAATAAAAAATGAATTAA
TTACGTTAGGAGACAATTAA

Downstream 100 bases:

>100_bases
ATTTAAGTAAAGGTTTTAATTATGAGAGGTGTTTTTTATTTACAAAGCTATTATTCTTTGTTGGACAGTTTATTACCTTT
AGAAAAATTAGTTTTAAAAG

Product: Lysyl-tRNA synthetase (class II)

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MNKKMTEQEGIRHQKIEELKKLKTEPFGKNFTCSHTIENIILNYQNIDQEWFEENKVNVKIAGRIILKRGQGKAGFLHLQ
DFNFKIQVYLRSDLLGDAFLLYQNCDLGDIVGIKGFLFKTKTKELTIKALDFIHLTKSLKPLPDKFHGLQNREEMRKKRY
LDLMVNEKSRQVFLTRSLIIKSIRNFFDNEGFLEVETPILQPSLGGASAKPFITSHNALNSDFYLRIATELPLKKLIVGG
MPKVYEIGRIFRNEGMDATHNPEFSTIEAYQAYSDIQGMMELTQKCLQYICQTVLKSLEINYQNKKISFQTFQKISMTDA
IKKETGIDFQKKLSQETCLQLAKQHDINVMSHFSQGHIIEVFFEKYVEHKLIQPTFIYGHPLEISPLAKKNLNNPRFTDR
FELFIAGKEFVNAFSELNDPIEQEKRFLNQLKQKELGDDEACEMDYDFLDALSYGMPPTGGLGMGIDRIVMLLTDTPNIR
DVILFPHFKNKNIIKKK

Sequences:

>Translated_497_residues
MNKKMTEQEGIRHQKIEELKKLKTEPFGKNFTCSHTIENIILNYQNIDQEWFEENKVNVKIAGRIILKRGQGKAGFLHLQ
DFNFKIQVYLRSDLLGDAFLLYQNCDLGDIVGIKGFLFKTKTKELTIKALDFIHLTKSLKPLPDKFHGLQNREEMRKKRY
LDLMVNEKSRQVFLTRSLIIKSIRNFFDNEGFLEVETPILQPSLGGASAKPFITSHNALNSDFYLRIATELPLKKLIVGG
MPKVYEIGRIFRNEGMDATHNPEFSTIEAYQAYSDIQGMMELTQKCLQYICQTVLKSLEINYQNKKISFQTFQKISMTDA
IKKETGIDFQKKLSQETCLQLAKQHDINVMSHFSQGHIIEVFFEKYVEHKLIQPTFIYGHPLEISPLAKKNLNNPRFTDR
FELFIAGKEFVNAFSELNDPIEQEKRFLNQLKQKELGDDEACEMDYDFLDALSYGMPPTGGLGMGIDRIVMLLTDTPNIR
DVILFPHFKNKNIIKKK
>Mature_497_residues
MNKKMTEQEGIRHQKIEELKKLKTEPFGKNFTCSHTIENIILNYQNIDQEWFEENKVNVKIAGRIILKRGQGKAGFLHLQ
DFNFKIQVYLRSDLLGDAFLLYQNCDLGDIVGIKGFLFKTKTKELTIKALDFIHLTKSLKPLPDKFHGLQNREEMRKKRY
LDLMVNEKSRQVFLTRSLIIKSIRNFFDNEGFLEVETPILQPSLGGASAKPFITSHNALNSDFYLRIATELPLKKLIVGG
MPKVYEIGRIFRNEGMDATHNPEFSTIEAYQAYSDIQGMMELTQKCLQYICQTVLKSLEINYQNKKISFQTFQKISMTDA
IKKETGIDFQKKLSQETCLQLAKQHDINVMSHFSQGHIIEVFFEKYVEHKLIQPTFIYGHPLEISPLAKKNLNNPRFTDR
FELFIAGKEFVNAFSELNDPIEQEKRFLNQLKQKELGDDEACEMDYDFLDALSYGMPPTGGLGMGIDRIVMLLTDTPNIR
DVILFPHFKNKNIIKKK

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=39.2857142857143, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI5031815, Length=504, Percent_Identity=39.2857142857143, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI226491502, Length=497, Percent_Identity=23.5412474849095, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI45439306, Length=461, Percent_Identity=22.9934924078091, Blast_Score=90, Evalue=4e-18,
Organism=Escherichia coli, GI1789256, Length=482, Percent_Identity=45.850622406639, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790571, Length=482, Percent_Identity=45.643153526971, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI87082379, Length=318, Percent_Identity=28.3018867924528, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1788173, Length=249, Percent_Identity=26.5060240963855, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17535925, Length=506, Percent_Identity=38.5375494071146, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17535927, Length=506, Percent_Identity=38.5375494071146, Blast_Score=348, Evalue=4e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=506, Percent_Identity=38.5375494071146, Blast_Score=347, Evalue=7e-96,
Organism=Caenorhabditis elegans, GI17551876, Length=528, Percent_Identity=21.5909090909091, Blast_Score=88, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320242, Length=504, Percent_Identity=38.8888888888889, Blast_Score=331, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6324256, Length=386, Percent_Identity=35.7512953367876, Blast_Score=210, Evalue=5e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=339, Percent_Identity=26.2536873156342, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=457, Percent_Identity=24.945295404814, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6325153, Length=137, Percent_Identity=32.1167883211679, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24640849, Length=501, Percent_Identity=38.12375249501, Blast_Score=338, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24640851, Length=501, Percent_Identity=38.12375249501, Blast_Score=338, Evalue=4e-93,
Organism=Drosophila melanogaster, GI17136276, Length=461, Percent_Identity=22.1258134490239, Blast_Score=82, Evalue=9e-16,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_PHYAS (B1VA97)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799121.1
- ProteinModelPortal:   B1VA97
- SMR:   B1VA97
- GeneID:   6798851
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG631383
- OMA:   VDMIANP
- ProtClustDB:   PRK00484
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 57608; Mature: 57608

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKMTEQEGIRHQKIEELKKLKTEPFGKNFTCSHTIENIILNYQNIDQEWFEENKVNVK
CCCCCCHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEE
IAGRIILKRGQGKAGFLHLQDFNFKIQVYLRSDLLGDAFLLYQNCDLGDIVGIKGFLFKT
EEEEEEEEECCCCEEEEEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEC
KTKELTIKALDFIHLTKSLKPLPDKFHGLQNREEMRKKRYLDLMVNEKSRQVFLTRSLII
CCCCEEHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHEECCCCCEEHHHHHHHH
KSIRNFFDNEGFLEVETPILQPSLGGASAKPFITSHNALNSDFYLRIATELPLKKLIVGG
HHHHHHHCCCCEEEECCCEECCCCCCCCCCCEEECCCCCCCCEEEEEECCCCHHHHHHCC
MPKVYEIGRIFRNEGMDATHNPEFSTIEAYQAYSDIQGMMELTQKCLQYICQTVLKSLEI
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NYQNKKISFQTFQKISMTDAIKKETGIDFQKKLSQETCLQLAKQHDINVMSHFSQGHIIE
CCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHCCCHHHHHCCCCCCHH
VFFEKYVEHKLIQPTFIYGHPLEISPLAKKNLNNPRFTDRFELFIAGKEFVNAFSELNDP
HHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHCCH
IEQEKRFLNQLKQKELGDDEACEMDYDFLDALSYGMPPTGGLGMGIDRIVMLLTDTPNIR
HHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCC
DVILFPHFKNKNIIKKK
EEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNKKMTEQEGIRHQKIEELKKLKTEPFGKNFTCSHTIENIILNYQNIDQEWFEENKVNVK
CCCCCCHHHCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCEEEE
IAGRIILKRGQGKAGFLHLQDFNFKIQVYLRSDLLGDAFLLYQNCDLGDIVGIKGFLFKT
EEEEEEEEECCCCEEEEEEECCCEEEEEEEECCCCCCEEEEEECCCCCCCCCCCCEEEEC
KTKELTIKALDFIHLTKSLKPLPDKFHGLQNREEMRKKRYLDLMVNEKSRQVFLTRSLII
CCCCEEHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHEECCCCCEEHHHHHHHH
KSIRNFFDNEGFLEVETPILQPSLGGASAKPFITSHNALNSDFYLRIATELPLKKLIVGG
HHHHHHHCCCCEEEECCCEECCCCCCCCCCCEEECCCCCCCCEEEEEECCCCHHHHHHCC
MPKVYEIGRIFRNEGMDATHNPEFSTIEAYQAYSDIQGMMELTQKCLQYICQTVLKSLEI
CCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NYQNKKISFQTFQKISMTDAIKKETGIDFQKKLSQETCLQLAKQHDINVMSHFSQGHIIE
CCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHCCCHHHHHCCCCCCHH
VFFEKYVEHKLIQPTFIYGHPLEISPLAKKNLNNPRFTDRFELFIAGKEFVNAFSELNDP
HHHHHHHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCCEEEEEEECHHHHHHHHHHCCH
IEQEKRFLNQLKQKELGDDEACEMDYDFLDALSYGMPPTGGLGMGIDRIVMLLTDTPNIR
HHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHCCCCCCCCCCCCHHHEEEEEECCCCCC
DVILFPHFKNKNIIKKK
EEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA