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The map label for this gene is tsf

Identifier: 197294563

GI number: 197294563

Start: 510973

End: 511806

Strand: Reverse

Name: tsf

Synonym: PAa_0518

Alternate gene names: 197294563

Gene position: 511806-510973 (Counterclockwise)

Preceding gene: 197294564

Following gene: 197294562

Centisome position: 58.16

GC content: 26.62

Gene sequence:

>834_bases
ATGAAAATTACAGCAGAAATGATTAAAGAATTAAGACAACAAACTCACGCTGGAATGATTGCATGCAAACAAGCGTTAGA
AAAAACAGAAGGAAATTTACAAAAAGCAATCGTTTTTTTAAGAGAAAAAGGAATTGTTAAAGCAAGCCAAAAGCAAGATA
GAACTACTTCAGAAGGTTTAATTAACATTGTTTTTTCTCAAAATGATGCTTTTTTATACGAATTAAATTCAGAAACTGAT
TTTGTTGCTAAAAATGAACATTTTCAGCAATTAATGAAAACGATAGGAGAAGTAATTCTTCAAAACAAACTACAAAGTGT
TGATGAAGTATTGACTTTTAACTATCAAAATAAAACTATCCAAGATTTGCTTTTAGAAAAAACATCAATTTTGGGTGAAA
AAATAACTCTTAAAAGAATATTAAAAGTTACTAAAAAAGAAGAAGAAATTTTTGGCACTTATAAACATCAAGGCGGTCGT
ATTTCTGTTTTGGTAGTGTTAGAAAATAATCATCCTTCAATTGCTGAAGATATAGCAATGCATATAGCCGCTTTTAATCC
TAAATTTTTAAACCCTGATAAAGTTAATCTTCAATTTTTAACTACTGAAAAAAATATTTTACAAAAACAAACCGAAAAAC
AGCTTTTAGAAGAAAAAAAACCTTTGCATATTTTAGATAAAATAGTTCAAAATCGTTTAAATAAACTTTTAAAAGAAATT
TGTTTATCAGAACAACCTTTCGTCAAAAACAACGAACAAAAAGTAAAAGACTATCTCCAAAACAATAATACAAATGTTGT
TTCTTATTTTCGTTGGTCAATCGCTAATCAATAA

Upstream 100 bases:

>100_bases
TTAAATTGTTTTCTTTAAAAAATACAAATAAATAACTTTTTTAAATACCTTTAAATTAATTTATTTTCAAAATGAATAAA
TGAAAGAAGCAGGCACCAAA

Downstream 100 bases:

>100_bases
TTTTCAGTTTTTTGAATAAAAATTATTCATGAAAAAAGGTCGTCAAATGTATAAAAAAATTCTTTTAAAATTAAGCGGTG
AGTCTCTTAAAGGAAATATT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MKITAEMIKELRQQTHAGMIACKQALEKTEGNLQKAIVFLREKGIVKASQKQDRTTSEGLINIVFSQNDAFLYELNSETD
FVAKNEHFQQLMKTIGEVILQNKLQSVDEVLTFNYQNKTIQDLLLEKTSILGEKITLKRILKVTKKEEEIFGTYKHQGGR
ISVLVVLENNHPSIAEDIAMHIAAFNPKFLNPDKVNLQFLTTEKNILQKQTEKQLLEEKKPLHILDKIVQNRLNKLLKEI
CLSEQPFVKNNEQKVKDYLQNNNTNVVSYFRWSIANQ

Sequences:

>Translated_277_residues
MKITAEMIKELRQQTHAGMIACKQALEKTEGNLQKAIVFLREKGIVKASQKQDRTTSEGLINIVFSQNDAFLYELNSETD
FVAKNEHFQQLMKTIGEVILQNKLQSVDEVLTFNYQNKTIQDLLLEKTSILGEKITLKRILKVTKKEEEIFGTYKHQGGR
ISVLVVLENNHPSIAEDIAMHIAAFNPKFLNPDKVNLQFLTTEKNILQKQTEKQLLEEKKPLHILDKIVQNRLNKLLKEI
CLSEQPFVKNNEQKVKDYLQNNNTNVVSYFRWSIANQ
>Mature_277_residues
MKITAEMIKELRQQTHAGMIACKQALEKTEGNLQKAIVFLREKGIVKASQKQDRTTSEGLINIVFSQNDAFLYELNSETD
FVAKNEHFQQLMKTIGEVILQNKLQSVDEVLTFNYQNKTIQDLLLEKTSILGEKITLKRILKVTKKEEEIFGTYKHQGGR
ISVLVVLENNHPSIAEDIAMHIAAFNPKFLNPDKVNLQFLTTEKNILQKQTEKQLLEEKKPLHILDKIVQNRLNKLLKEI
CLSEQPFVKNNEQKVKDYLQNNNTNVVSYFRWSIANQ

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Homo sapiens, GI171846268, Length=218, Percent_Identity=26.605504587156, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI291084500, Length=94, Percent_Identity=38.2978723404255, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI291084498, Length=94, Percent_Identity=38.2978723404255, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI291084502, Length=94, Percent_Identity=38.2978723404255, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786366, Length=276, Percent_Identity=31.8840579710145, Blast_Score=159, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17561440, Length=308, Percent_Identity=25.6493506493506, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_PHYAS (B1VA79)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799104.1
- ProteinModelPortal:   B1VA79
- SMR:   B1VA79
- GeneID:   6799282
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 32011; Mature: 32011

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITAEMIKELRQQTHAGMIACKQALEKTEGNLQKAIVFLREKGIVKASQKQDRTTSEGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCE
INIVFSQNDAFLYELNSETDFVAKNEHFQQLMKTIGEVILQNKLQSVDEVLTFNYQNKTI
EEEEEECCCEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QDLLLEKTSILGEKITLKRILKVTKKEEEIFGTYKHQGGRISVLVVLENNHPSIAEDIAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHH
HIAAFNPKFLNPDKVNLQFLTTEKNILQKQTEKQLLEEKKPLHILDKIVQNRLNKLLKEI
HHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
CLSEQPFVKNNEQKVKDYLQNNNTNVVSYFRWSIANQ
HCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEHHCCC
>Mature Secondary Structure
MKITAEMIKELRQQTHAGMIACKQALEKTEGNLQKAIVFLREKGIVKASQKQDRTTSEGL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHCCCCCCCCE
INIVFSQNDAFLYELNSETDFVAKNEHFQQLMKTIGEVILQNKLQSVDEVLTFNYQNKTI
EEEEEECCCEEEEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
QDLLLEKTSILGEKITLKRILKVTKKEEEIFGTYKHQGGRISVLVVLENNHPSIAEDIAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHH
HIAAFNPKFLNPDKVNLQFLTTEKNILQKQTEKQLLEEKKPLHILDKIVQNRLNKLLKEI
HHHHCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
CLSEQPFVKNNEQKVKDYLQNNNTNVVSYFRWSIANQ
HCCCCCCCCCCHHHHHHHHHCCCCCEEEEEEEHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA