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The map label for this gene is bcgIA [H]

Identifier: 197294500

GI number: 197294500

Start: 432859

End: 434622

Strand: Direct

Name: bcgIA [H]

Synonym: PAa_0438

Alternate gene names: 197294500

Gene position: 432859-434622 (Clockwise)

Preceding gene: 197294499

Following gene: 197294501

Centisome position: 49.19

GC content: 28.57

Gene sequence:

>1764_bases
ATGAACGAAAGAATAACAGAAGCAATCACTAAAGAATTAGTTTACAAAACTTATAATAAAGAAGAAATTGTTTTTGATGA
ACAAATTAGCAGCATTAAAGGAATCAAAAAACTACTAGGAAATCAAAAACCTGATTTTATTATTTATTTTAAAGATAACC
AAGACAACCTTAATATTATTGTTATTATCGAATGCAAGGCTGATACTAAAAAACAAGAAGAAGCCAATTCTCAAGCAAAA
AAATATGCTAAATCTTTAAAATCAAAATATAACGTTTTTTATTATGGTATATCAGGACAAAATAACCAAAGGAATCTAAT
TGAAGGCCGTTTGTGGATCAAAAACAACCAACAACCATTTCCAGATAAATATAAAAAATTATTAAAATATCAAGATATAA
AAAAAGAAATATACAAATTAACTAATTCTTCAAACCGCTTCAATTATGATAGTTTAAAAATTTTTAAGGAATTTAATAAA
AAATTATACAAAATGGGAATCCCTTCATCTGAAAGACCTGTTTTAGTTGGTTGTTTTTTAATTTCACTTTTAGGAAACGT
TAATATTTGTGATAATAATAATGAATATTTAAAAAACATCATCATTACTGCTGCTAAAAACAAGTTAAAAAAAATAGATC
ACGAAAAAAGAGAAGATATAGGAAGTCATTATAATAGATTACTTTCTAACGCCATGTGGACGTCTCAAGAAGAAGTAGAT
AGTTATGGTAATAAAATTCCAAATAACAATTTAGTCTATTTTCTAACTGAATTAAAAGAAAAAATTATGCCTTTTATTCA
CAGTGACCAATGGGATGTTATGGGAACTTTTTATCGTGAGTTTATTAAATACGTTACCGAAGATAAGCAAACAGGATTAG
TTTTAACACCACCTCATATTACAGATTTCTTCTGTGAATTAGCTGATATTCAAAGTTCCGATATTGTTTTGGACCCATGT
TGTGGCACAGGGGGATTTTTAATTGCAGCGATGAAATATATGTGCCAAAAAGCCAAAAATGAAAAACAAATAGAAGTGAT
CAAAACTAAACAATTGTTAGGAATTGAAAAAAGAAAAGATATGTGGCTACATGCAAGTGTTAATATGATGATGCACGGTG
ATGGACATACTAATATTTTTTATGGCGATTGTTTCAAATTTAAAATTGACAATTTCAAACATAACCAACCAACCGTAGTT
TTTTTAAACCCCCCTTATAATGAACCCTCTGAACAATTAAGGTTTATCCAAAAAGCTTTAGAATTGACAACCCCCAAAGG
ACAAGTAATTGCCATCGTACAGGCTTCCGCTACTGGCCAATCTACAGCTGTCAATAATGCCAAAAAAGAAATCTTAAATA
ACCATACTTTATTGGCTAGCTTTTCATGTCCTAAAGAACTTTTTCACGGCATTGCTGGTGTAATCACTAATATTTTAATT
TTTGCAGCCCATGTACCCCATGATTCTAAAAAAAATACTTTTTTAGGATGGTTTAAAGATGATGGCTTCATCAAACAAAA
AAACAAAAGAATTGAAAAAAATTGGAAATTAATTAAGGATAAATGGATTGACATATATAGGAACAAAAAAGAGGTTAAAG
AAACTAATATAGAAAGCAAAATGCAAACACTTACTCATGAGGACGAATGGTGCATAGAAGCATACTTAAAACCTGAATTA
CCTTCTCAAAAAGATTTTATGTTGATTATTAAAGAGTATATTTTACACGAATTTGAGGTTTTATTAGACAATGAAATTAA
CTGA

Upstream 100 bases:

>100_bases
GAGCTTATTACCAAAATTAATTATTCCATTTTGAATTCAAACAAAATACAATTTTTTTAGATAAGTTTTATTCAAATCAC
AAATTCGAAAGAGGGAAATA

Downstream 100 bases:

>100_bases
GATTTTTAATGTATTACAAGGGAACAAACTGCACGCTAATAAAGTTATTGAAGATGCACAAGGAATTAATTTAATTAGCA
GAAAAGGCTCTAATTATGGA

Product: Restriction enzyme alpha subunit

Products: NA

Alternate protein names: Adenine-specific methyltransferase activity [H]

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNERITEAITKELVYKTYNKEEIVFDEQISSIKGIKKLLGNQKPDFIIYFKDNQDNLNIIVIIECKADTKKQEEANSQAK
KYAKSLKSKYNVFYYGISGQNNQRNLIEGRLWIKNNQQPFPDKYKKLLKYQDIKKEIYKLTNSSNRFNYDSLKIFKEFNK
KLYKMGIPSSERPVLVGCFLISLLGNVNICDNNNEYLKNIIITAAKNKLKKIDHEKREDIGSHYNRLLSNAMWTSQEEVD
SYGNKIPNNNLVYFLTELKEKIMPFIHSDQWDVMGTFYREFIKYVTEDKQTGLVLTPPHITDFFCELADIQSSDIVLDPC
CGTGGFLIAAMKYMCQKAKNEKQIEVIKTKQLLGIEKRKDMWLHASVNMMMHGDGHTNIFYGDCFKFKIDNFKHNQPTVV
FLNPPYNEPSEQLRFIQKALELTTPKGQVIAIVQASATGQSTAVNNAKKEILNNHTLLASFSCPKELFHGIAGVITNILI
FAAHVPHDSKKNTFLGWFKDDGFIKQKNKRIEKNWKLIKDKWIDIYRNKKEVKETNIESKMQTLTHEDEWCIEAYLKPEL
PSQKDFMLIIKEYILHEFEVLLDNEIN

Sequences:

>Translated_587_residues
MNERITEAITKELVYKTYNKEEIVFDEQISSIKGIKKLLGNQKPDFIIYFKDNQDNLNIIVIIECKADTKKQEEANSQAK
KYAKSLKSKYNVFYYGISGQNNQRNLIEGRLWIKNNQQPFPDKYKKLLKYQDIKKEIYKLTNSSNRFNYDSLKIFKEFNK
KLYKMGIPSSERPVLVGCFLISLLGNVNICDNNNEYLKNIIITAAKNKLKKIDHEKREDIGSHYNRLLSNAMWTSQEEVD
SYGNKIPNNNLVYFLTELKEKIMPFIHSDQWDVMGTFYREFIKYVTEDKQTGLVLTPPHITDFFCELADIQSSDIVLDPC
CGTGGFLIAAMKYMCQKAKNEKQIEVIKTKQLLGIEKRKDMWLHASVNMMMHGDGHTNIFYGDCFKFKIDNFKHNQPTVV
FLNPPYNEPSEQLRFIQKALELTTPKGQVIAIVQASATGQSTAVNNAKKEILNNHTLLASFSCPKELFHGIAGVITNILI
FAAHVPHDSKKNTFLGWFKDDGFIKQKNKRIEKNWKLIKDKWIDIYRNKKEVKETNIESKMQTLTHEDEWCIEAYLKPEL
PSQKDFMLIIKEYILHEFEVLLDNEIN
>Mature_587_residues
MNERITEAITKELVYKTYNKEEIVFDEQISSIKGIKKLLGNQKPDFIIYFKDNQDNLNIIVIIECKADTKKQEEANSQAK
KYAKSLKSKYNVFYYGISGQNNQRNLIEGRLWIKNNQQPFPDKYKKLLKYQDIKKEIYKLTNSSNRFNYDSLKIFKEFNK
KLYKMGIPSSERPVLVGCFLISLLGNVNICDNNNEYLKNIIITAAKNKLKKIDHEKREDIGSHYNRLLSNAMWTSQEEVD
SYGNKIPNNNLVYFLTELKEKIMPFIHSDQWDVMGTFYREFIKYVTEDKQTGLVLTPPHITDFFCELADIQSSDIVLDPC
CGTGGFLIAAMKYMCQKAKNEKQIEVIKTKQLLGIEKRKDMWLHASVNMMMHGDGHTNIFYGDCFKFKIDNFKHNQPTVV
FLNPPYNEPSEQLRFIQKALELTTPKGQVIAIVQASATGQSTAVNNAKKEILNNHTLLASFSCPKELFHGIAGVITNILI
FAAHVPHDSKKNTFLGWFKDDGFIKQKNKRIEKNWKLIKDKWIDIYRNKKEVKETNIESKMQTLTHEDEWCIEAYLKPEL
PSQKDFMLIIKEYILHEFEVLLDNEIN

Specific function: Recognizes the double-stranded sequence 5'-CGAN(6)TGC-3' and cleaves bilaterally and symmetrically outside the sequence to release a 34-base pair fragment. Methylation of the recognition sequence occurs on the adenine in either one or both strands [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 68403; Mature: 68403

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERITEAITKELVYKTYNKEEIVFDEQISSIKGIKKLLGNQKPDFIIYFKDNQDNLNII
CCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
VIIECKADTKKQEEANSQAKKYAKSLKSKYNVFYYGISGQNNQRNLIEGRLWIKNNQQPF
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCC
PDKYKKLLKYQDIKKEIYKLTNSSNRFNYDSLKIFKEFNKKLYKMGIPSSERPVLVGCFL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ISLLGNVNICDNNNEYLKNIIITAAKNKLKKIDHEKREDIGSHYNRLLSNAMWTSQEEVD
HHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHH
SYGNKIPNNNLVYFLTELKEKIMPFIHSDQWDVMGTFYREFIKYVTEDKQTGLVLTPPHI
HHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCHH
TDFFCELADIQSSDIVLDPCCGTGGFLIAAMKYMCQKAKNEKQIEVIKTKQLLGIEKRKD
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCHHHCC
MWLHASVNMMMHGDGHTNIFYGDCFKFKIDNFKHNQPTVVFLNPPYNEPSEQLRFIQKAL
EEEEEEEEEEEECCCCCEEEECCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
ELTTPKGQVIAIVQASATGQSTAVNNAKKEILNNHTLLASFSCPKELFHGIAGVITNILI
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
FAAHVPHDSKKNTFLGWFKDDGFIKQKNKRIEKNWKLIKDKWIDIYRNKKEVKETNIESK
HHEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MQTLTHEDEWCIEAYLKPELPSQKDFMLIIKEYILHEFEVLLDNEIN
HHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNERITEAITKELVYKTYNKEEIVFDEQISSIKGIKKLLGNQKPDFIIYFKDNQDNLNII
CCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEE
VIIECKADTKKQEEANSQAKKYAKSLKSKYNVFYYGISGQNNQRNLIEGRLWIKNNQQPF
EEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEEEEEEECCCCCC
PDKYKKLLKYQDIKKEIYKLTNSSNRFNYDSLKIFKEFNKKLYKMGIPSSERPVLVGCFL
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
ISLLGNVNICDNNNEYLKNIIITAAKNKLKKIDHEKREDIGSHYNRLLSNAMWTSQEEVD
HHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHH
SYGNKIPNNNLVYFLTELKEKIMPFIHSDQWDVMGTFYREFIKYVTEDKQTGLVLTPPHI
HHCCCCCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCHH
TDFFCELADIQSSDIVLDPCCGTGGFLIAAMKYMCQKAKNEKQIEVIKTKQLLGIEKRKD
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCHHHCC
MWLHASVNMMMHGDGHTNIFYGDCFKFKIDNFKHNQPTVVFLNPPYNEPSEQLRFIQKAL
EEEEEEEEEEEECCCCCEEEECCEEEEEECCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
ELTTPKGQVIAIVQASATGQSTAVNNAKKEILNNHTLLASFSCPKELFHGIAGVITNILI
HCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
FAAHVPHDSKKNTFLGWFKDDGFIKQKNKRIEKNWKLIKDKWIDIYRNKKEVKETNIESK
HHEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
MQTLTHEDEWCIEAYLKPELPSQKDFMLIIKEYILHEFEVLLDNEIN
HHHHCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8451198; 8276869 [H]