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The map label for this gene is glyA

Identifier: 197294309

GI number: 197294309

Start: 209076

End: 210317

Strand: Reverse

Name: glyA

Synonym: PAa_0172

Alternate gene names: 197294309

Gene position: 210317-209076 (Counterclockwise)

Preceding gene: 197294310

Following gene: 197294308

Centisome position: 23.9

GC content: 31.4

Gene sequence:

>1242_bases
ATGAAATCACAATTAAGTTTAAAAGATCCAAAAATATTTGAACTAATCGAACAAGAAAAAAAAAGACAAAAAGAAAATAT
CATATTAATTGCTTCCGAAAACTTCGTTTCCAAAGAAGTTTTAGAAACTCAAGGTAGTATTTTAACTAATAAATACGCCG
AAGGATATCCTGGAAAAAGATATTATCATGGTTGTGGTAATGTAGATGATATTGAACAAATAGCCATCGAAAGGGCGAAA
AAACTCTTTAATGCTCGTTATGCCAACGTTCAACCGCATTCAGGTTCTCAAGCTAATATGGCAGTTTTACAAGCTTTATT
GCAACCTAACGATAAAATTTTAAGTTTGTCTTTAAACGATGGTGGGCATTTAACTCACGGACATAAATTAAGTTTTTCAG
GTAAATATTATCAATCTTATTCTTATAATGTCGATCCAACAACAGAAATGCTTGATTATGAATCAATTAGAAAACTGGCT
TTGGAAATCAAACCAAAATTAATTATCGCAGGATATTCTGCTTATTCCAGAAAAATTAATTTTCAAAAATTCCGTGAGAT
TGCTAATGAAGTCAATGCTTATTTAATGGCTGATATTGCTCATATTGCAGGCTTTGTTGCATGCAAATTACACCCTTGTC
CTTTAGAAGCTCAAGCAGATATAGTTACTTCAACGACTCACAAAACTTTAAGAGGACCTCGGGGGGGATTGATTTTAACC
AACAAAGAAAAAATAATGCAACAAATTAATAGATCTGTTTTTCCGGGAATTCAAGGAGGGCCTTTGATGCATGTTATTGC
TGCTAAAGCTGTTTCCTTCAAAGAAGCTCAAAGTTTAGAATTTAAAAATTATCAACAACAAGTAATTAAAAACGCTCAAG
CATTTGCTCAAACTTTTCAAAAAAAAGGTTATCACGTTGTAAGTCAAGGAACTGATAATCATTTATTTTTAATTAATTTA
AAAAAAACAAACCCTCTTTTTACCGGAGAAAAAATTGCAAACATTTTAGAAAAAGTTAACATTATAGTCAACAAAAACAC
TATTCCTTTTGATCAAGAAAAACCAATGTTTACTAGTGGTATTAGGTTGGGAACGCCTGCCATGACAACAAAAGGATTTC
AAGAAGCAGATTTCATTAAGTTGGCAGATTTGATTGATCAGGCCATCAAAAATAGGGACGACAACGTGTATCTGCAAAAA
ATAAAAAAAGAAGTTCTAGATTGGACAAATGATTTTAAATAA

Upstream 100 bases:

>100_bases
ATTAAAAGATTTTTTCTTAATGTTTGTTGTTTGCATACTTTTTTTAATCAAATATTTGTGCAGTTAATAAATAAAATAAG
AAAAGAGTGTTAATAATTTT

Downstream 100 bases:

>100_bases
TATCTCAATTCATCCCAAAATAGAAAAAATCTCAACCGCAAAGATATTTTTTAATTAAATAAAAGTAAATTTTGCAGTTT
TTGAGTAAGAAAAAACAGCC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MKSQLSLKDPKIFELIEQEKKRQKENIILIASENFVSKEVLETQGSILTNKYAEGYPGKRYYHGCGNVDDIEQIAIERAK
KLFNARYANVQPHSGSQANMAVLQALLQPNDKILSLSLNDGGHLTHGHKLSFSGKYYQSYSYNVDPTTEMLDYESIRKLA
LEIKPKLIIAGYSAYSRKINFQKFREIANEVNAYLMADIAHIAGFVACKLHPCPLEAQADIVTSTTHKTLRGPRGGLILT
NKEKIMQQINRSVFPGIQGGPLMHVIAAKAVSFKEAQSLEFKNYQQQVIKNAQAFAQTFQKKGYHVVSQGTDNHLFLINL
KKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSGIRLGTPAMTTKGFQEADFIKLADLIDQAIKNRDDNVYLQK
IKKEVLDWTNDFK

Sequences:

>Translated_413_residues
MKSQLSLKDPKIFELIEQEKKRQKENIILIASENFVSKEVLETQGSILTNKYAEGYPGKRYYHGCGNVDDIEQIAIERAK
KLFNARYANVQPHSGSQANMAVLQALLQPNDKILSLSLNDGGHLTHGHKLSFSGKYYQSYSYNVDPTTEMLDYESIRKLA
LEIKPKLIIAGYSAYSRKINFQKFREIANEVNAYLMADIAHIAGFVACKLHPCPLEAQADIVTSTTHKTLRGPRGGLILT
NKEKIMQQINRSVFPGIQGGPLMHVIAAKAVSFKEAQSLEFKNYQQQVIKNAQAFAQTFQKKGYHVVSQGTDNHLFLINL
KKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSGIRLGTPAMTTKGFQEADFIKLADLIDQAIKNRDDNVYLQK
IKKEVLDWTNDFK
>Mature_413_residues
MKSQLSLKDPKIFELIEQEKKRQKENIILIASENFVSKEVLETQGSILTNKYAEGYPGKRYYHGCGNVDDIEQIAIERAK
KLFNARYANVQPHSGSQANMAVLQALLQPNDKILSLSLNDGGHLTHGHKLSFSGKYYQSYSYNVDPTTEMLDYESIRKLA
LEIKPKLIIAGYSAYSRKINFQKFREIANEVNAYLMADIAHIAGFVACKLHPCPLEAQADIVTSTTHKTLRGPRGGLILT
NKEKIMQQINRSVFPGIQGGPLMHVIAAKAVSFKEAQSLEFKNYQQQVIKNAQAFAQTFQKKGYHVVSQGTDNHLFLINL
KKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSGIRLGTPAMTTKGFQEADFIKLADLIDQAIKNRDDNVYLQK
IKKEVLDWTNDFK

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=411, Percent_Identity=46.2287104622871, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI261862352, Length=411, Percent_Identity=46.2287104622871, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI261862350, Length=411, Percent_Identity=46.2287104622871, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI261862348, Length=411, Percent_Identity=46.2287104622871, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI261862346, Length=411, Percent_Identity=45.2554744525547, Blast_Score=347, Evalue=9e-96,
Organism=Homo sapiens, GI22547186, Length=405, Percent_Identity=44.1975308641975, Blast_Score=322, Evalue=5e-88,
Organism=Homo sapiens, GI22547189, Length=391, Percent_Identity=42.1994884910486, Blast_Score=290, Evalue=2e-78,
Organism=Escherichia coli, GI1788902, Length=401, Percent_Identity=54.6134663341646, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI25144732, Length=404, Percent_Identity=44.8019801980198, Blast_Score=359, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI25144729, Length=404, Percent_Identity=44.8019801980198, Blast_Score=358, Evalue=2e-99,
Organism=Saccharomyces cerevisiae, GI6319739, Length=409, Percent_Identity=47.6772616136919, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323087, Length=457, Percent_Identity=41.5754923413567, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24640005, Length=412, Percent_Identity=47.5728155339806, Blast_Score=380, Evalue=1e-106,
Organism=Drosophila melanogaster, GI221329721, Length=412, Percent_Identity=47.5728155339806, Blast_Score=380, Evalue=1e-106,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_PHYAS (B1V975)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001798850.1
- ProteinModelPortal:   B1V975
- SMR:   B1V975
- GeneID:   6799053
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG301263
- OMA:   MASDVAK
- ProtClustDB:   PRK00011
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 46630; Mature: 46630

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSQLSLKDPKIFELIEQEKKRQKENIILIASENFVSKEVLETQGSILTNKYAEGYPGKR
CCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCC
YYHGCGNVDDIEQIAIERAKKLFNARYANVQPHSGSQANMAVLQALLQPNDKILSLSLND
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECC
GGHLTHGHKLSFSGKYYQSYSYNVDPTTEMLDYESIRKLALEIKPKLIIAGYSAYSRKIN
CCCCCCCCEEECCCCEEECCCCCCCCHHHHHCHHHHHHHHHHCCCEEEEEECHHHHHCCC
FQKFREIANEVNAYLMADIAHIAGFVACKLHPCPLEAQADIVTSTTHKTLRGPRGGLILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEECCHHHHHCCCCCCEEEE
NKEKIMQQINRSVFPGIQGGPLMHVIAAKAVSFKEAQSLEFKNYQQQVIKNAQAFAQTFQ
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KKGYHVVSQGTDNHLFLINLKKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSG
HCCCEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCHHCC
IRLGTPAMTTKGFQEADFIKLADLIDQAIKNRDDNVYLQKIKKEVLDWTNDFK
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKSQLSLKDPKIFELIEQEKKRQKENIILIASENFVSKEVLETQGSILTNKYAEGYPGKR
CCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCHHHHHHCCCCCCCC
YYHGCGNVDDIEQIAIERAKKLFNARYANVQPHSGSQANMAVLQALLQPNDKILSLSLND
EECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECC
GGHLTHGHKLSFSGKYYQSYSYNVDPTTEMLDYESIRKLALEIKPKLIIAGYSAYSRKIN
CCCCCCCCEEECCCCEEECCCCCCCCHHHHHCHHHHHHHHHHCCCEEEEEECHHHHHCCC
FQKFREIANEVNAYLMADIAHIAGFVACKLHPCPLEAQADIVTSTTHKTLRGPRGGLILT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCEECCHHHHHCCCCCCEEEE
NKEKIMQQINRSVFPGIQGGPLMHVIAAKAVSFKEAQSLEFKNYQQQVIKNAQAFAQTFQ
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
KKGYHVVSQGTDNHLFLINLKKTNPLFTGEKIANILEKVNIIVNKNTIPFDQEKPMFTSG
HCCCEEEECCCCCEEEEEEEECCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCHHCC
IRLGTPAMTTKGFQEADFIKLADLIDQAIKNRDDNVYLQKIKKEVLDWTNDFK
EECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA