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The map label for this gene is pfkA [H]

Identifier: 197294301

GI number: 197294301

Start: 194757

End: 195731

Strand: Direct

Name: pfkA [H]

Synonym: PAa_0164

Alternate gene names: 197294301

Gene position: 194757-195731 (Clockwise)

Preceding gene: 197294295

Following gene: 197294302

Centisome position: 22.13

GC content: 32.31

Gene sequence:

>975_bases
ATGAAAAAAGAAAAAGAATTAAAAAAAATTGCTGTTTTAACTTCAGGAGGAGATGCTCCTGGTATGAATGCTGCCATTAG
AGCAGTTGTTTTAGAAGGAAATAAACAAGGTTTTGAAGTTTATGGAGTTAAAGATGGTTATTTAGGTTTGTATAATAACG
AAATAAGTCTTTTAGATTCAAATTATTTCCCCCGCGTTATTAATGTTTCAGGAACTTTTTTGGGAACTTCTCGTTTTCTC
CCTTTTCAAAACGACCTTGCTGTTCGCCAAACTTGTGCAAACAATTTGAAAAAATTGGGAGTTGAAAAATTAGTTGTTAT
CGGTGGTGACGGTTCTTATAAAGGCGCTATGAAATTAAAAGAATTAGGAATTAAATGTGTTGGCTTGCCTGCTACTATTG
ATAATGATATTAATAATACCGATTTTACAATTGGTTTTAGTACTGCTTTGAATAACATTGTGGATGCAATTGAAAAATTA
CGCGATACTTCTATTTCTCATCATAGATGTAGTATCATTGAAGTTATGGGACGCTACAAAGGAGATTTAGCTTTATACGG
AGGACTTGCAGCCGCTTCAGATGTTATTGTAACTCGCGAAAATCTTTTATCTAAACAAGAAATTTTAGACAAAGTTAAAG
TTTTATATCAAGCTAAAAAAAGACATGCTATTGTAGTTGTGACAGAACATATCTTTGATGTTGCAACCCTTGCTAAGGAA
GTTGAAAAATATAGCGGATTTGAAACTCGCGCCCAAGTTTTAGGTCATATTCAAAGAGGCGGAAGACCTACTGCAGAAGA
CTTAGTGCTTGCTTTTAGAATGGGAAGTTATGCGGTTTCTTTGTTACAACAAAATATTTTTGATTGTGCTGTTGGTACTT
GTGGTCTTAATTTAAATTATACACATTTTTTTGATGTTTTTAATAACCATAACCAACAAAATGATTTATTTCATGCAATT
GCTAAATTATTATAA

Upstream 100 bases:

>100_bases
TTTATTAATTAATTCAATTCATTAATCTTTTAATCACTTAAGTTAGTTATAAGTACATGTTTTGAAAATAAAATAAATTT
AAAGAGGTTTTGTATCAAAA

Downstream 100 bases:

>100_bases
ACATAATTTATATAGTTTTTTTTCTGGCTAACTAAAATAAATATTTTTAAATTAAGACAAATTCTATTTTATTTAGATTT
CAAATGTCATTTTTTTTTGG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKKEKELKKIAVLTSGGDAPGMNAAIRAVVLEGNKQGFEVYGVKDGYLGLYNNEISLLDSNYFPRVINVSGTFLGTSRFL
PFQNDLAVRQTCANNLKKLGVEKLVVIGGDGSYKGAMKLKELGIKCVGLPATIDNDINNTDFTIGFSTALNNIVDAIEKL
RDTSISHHRCSIIEVMGRYKGDLALYGGLAAASDVIVTRENLLSKQEILDKVKVLYQAKKRHAIVVVTEHIFDVATLAKE
VEKYSGFETRAQVLGHIQRGGRPTAEDLVLAFRMGSYAVSLLQQNIFDCAVGTCGLNLNYTHFFDVFNNHNQQNDLFHAI
AKLL

Sequences:

>Translated_324_residues
MKKEKELKKIAVLTSGGDAPGMNAAIRAVVLEGNKQGFEVYGVKDGYLGLYNNEISLLDSNYFPRVINVSGTFLGTSRFL
PFQNDLAVRQTCANNLKKLGVEKLVVIGGDGSYKGAMKLKELGIKCVGLPATIDNDINNTDFTIGFSTALNNIVDAIEKL
RDTSISHHRCSIIEVMGRYKGDLALYGGLAAASDVIVTRENLLSKQEILDKVKVLYQAKKRHAIVVVTEHIFDVATLAKE
VEKYSGFETRAQVLGHIQRGGRPTAEDLVLAFRMGSYAVSLLQQNIFDCAVGTCGLNLNYTHFFDVFNNHNQQNDLFHAI
AKLL
>Mature_324_residues
MKKEKELKKIAVLTSGGDAPGMNAAIRAVVLEGNKQGFEVYGVKDGYLGLYNNEISLLDSNYFPRVINVSGTFLGTSRFL
PFQNDLAVRQTCANNLKKLGVEKLVVIGGDGSYKGAMKLKELGIKCVGLPATIDNDINNTDFTIGFSTALNNIVDAIEKL
RDTSISHHRCSIIEVMGRYKGDLALYGGLAAASDVIVTRENLLSKQEILDKVKVLYQAKKRHAIVVVTEHIFDVATLAKE
VEKYSGFETRAQVLGHIQRGGRPTAEDLVLAFRMGSYAVSLLQQNIFDCAVGTCGLNLNYTHFFDVFNNHNQQNDLFHAI
AKLL

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=330, Percent_Identity=36.0606060606061, Blast_Score=168, Evalue=7e-42,
Organism=Homo sapiens, GI266453619, Length=326, Percent_Identity=35.2760736196319, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI266453768, Length=326, Percent_Identity=35.2760736196319, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI266453748, Length=326, Percent_Identity=35.2760736196319, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4505749, Length=326, Percent_Identity=35.2760736196319, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI48762920, Length=326, Percent_Identity=34.3558282208589, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI1790350, Length=319, Percent_Identity=49.8432601880878, Blast_Score=305, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17558788, Length=314, Percent_Identity=33.7579617834395, Blast_Score=157, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI25147584, Length=315, Percent_Identity=34.6031746031746, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI72003601, Length=296, Percent_Identity=28.7162162162162, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321679, Length=312, Percent_Identity=36.2179487179487, Blast_Score=156, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323861, Length=334, Percent_Identity=34.1317365269461, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24652337, Length=317, Percent_Identity=36.9085173501577, Blast_Score=167, Evalue=7e-42,
Organism=Drosophila melanogaster, GI17647809, Length=317, Percent_Identity=36.9085173501577, Blast_Score=167, Evalue=8e-42,
Organism=Drosophila melanogaster, GI28573326, Length=316, Percent_Identity=35.7594936708861, Blast_Score=167, Evalue=9e-42,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35591; Mature: 35591

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKEKELKKIAVLTSGGDAPGMNAAIRAVVLEGNKQGFEVYGVKDGYLGLYNNEISLLDS
CCCHHHHHEEEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEECCCEEEEECCEEEEEEC
NYFPRVINVSGTFLGTSRFLPFQNDLAVRQTCANNLKKLGVEKLVVIGGDGSYKGAMKLK
CCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
ELGIKCVGLPATIDNDINNTDFTIGFSTALNNIVDAIEKLRDTSISHHRCSIIEVMGRYK
HCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GDLALYGGLAAASDVIVTRENLLSKQEILDKVKVLYQAKKRHAIVVVTEHIFDVATLAKE
CCEEEECCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHH
VEKYSGFETRAQVLGHIQRGGRPTAEDLVLAFRMGSYAVSLLQQNIFDCAVGTCGLNLNY
HHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
THFFDVFNNHNQQNDLFHAIAKLL
HHHHHHHCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MKKEKELKKIAVLTSGGDAPGMNAAIRAVVLEGNKQGFEVYGVKDGYLGLYNNEISLLDS
CCCHHHHHEEEEEECCCCCCCCCCEEEEEEEECCCCCEEEEEECCCEEEEECCEEEEEEC
NYFPRVINVSGTFLGTSRFLPFQNDLAVRQTCANNLKKLGVEKLVVIGGDGSYKGAMKLK
CCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHH
ELGIKCVGLPATIDNDINNTDFTIGFSTALNNIVDAIEKLRDTSISHHRCSIIEVMGRYK
HCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GDLALYGGLAAASDVIVTRENLLSKQEILDKVKVLYQAKKRHAIVVVTEHIFDVATLAKE
CCEEEECCHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHH
VEKYSGFETRAQVLGHIQRGGRPTAEDLVLAFRMGSYAVSLLQQNIFDCAVGTCGLNLNY
HHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
THFFDVFNNHNQQNDLFHAIAKLL
HHHHHHHCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]