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The map label for this gene is mscL [H]

Identifier: 197294250

GI number: 197294250

Start: 123394

End: 123798

Strand: Direct

Name: mscL [H]

Synonym: PAa_0113

Alternate gene names: 197294250

Gene position: 123394-123798 (Clockwise)

Preceding gene: 197294249

Following gene: 197294251

Centisome position: 14.02

GC content: 25.93

Gene sequence:

>405_bases
TTGACGTTTTTTCAAGGATTTAAAAATTTTATCACCCGTGGCAATGTAATTAATTTAGCAGTTGCAGTTGTTATAGGTCA
ATTGTTTTCTAAGATAGTGAGCTCTTTGGTAGCAGATATTATGATGCCTCCTTTTTCTTTATTGTTTAACGAAACTAAAG
GTTTACAAGGTCTAAAATGGTGTATCAAAGAAGACGTTTATATGAATTACGGTATTTTTTTGCAAAATATTTTAGAATTC
GTTATTTTTTCTTTTGCTATTTATCTGATTTTAACTATTTTTTCAAGAACTAAATTGGAAAAACCTAAAAGCGAATTAAA
ACTTATCTTAGAAAGTTTACAAAAAGAAATTACTTTATTACAAGAAATTAAAAACAATTTACAACAAAACAACAACAAGA
AGTAA

Upstream 100 bases:

>100_bases
AAAATTATAAAACTTCAAGACTTTATTTTATCACTTTCTTATTTTGAATAATGTATAAGGAGAATTTATTATTAATAATT
ATAATTTAGCAACAAAATCT

Downstream 100 bases:

>100_bases
TAAAAAACCAACCCAAAAGACAAATTTACAATCCAACAAAAAAAGGCTATTAATAGTCTTTTTTATTTACTTTTTTATTT
TTTTAAACTTATTATTTTAT

Product: Putative large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MTFFQGFKNFITRGNVINLAVAVVIGQLFSKIVSSLVADIMMPPFSLLFNETKGLQGLKWCIKEDVYMNYGIFLQNILEF
VIFSFAIYLILTIFSRTKLEKPKSELKLILESLQKEITLLQEIKNNLQQNNNKK

Sequences:

>Translated_134_residues
MTFFQGFKNFITRGNVINLAVAVVIGQLFSKIVSSLVADIMMPPFSLLFNETKGLQGLKWCIKEDVYMNYGIFLQNILEF
VIFSFAIYLILTIFSRTKLEKPKSELKLILESLQKEITLLQEIKNNLQQNNNKK
>Mature_133_residues
TFFQGFKNFITRGNVINLAVAVVIGQLFSKIVSSLVADIMMPPFSLLFNETKGLQGLKWCIKEDVYMNYGIFLQNILEFV
IFSFAIYLILTIFSRTKLEKPKSELKLILESLQKEITLLQEIKNNLQQNNNKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=141, Percent_Identity=39.0070921985816, Blast_Score=96, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 15472; Mature: 15340

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFFQGFKNFITRGNVINLAVAVVIGQLFSKIVSSLVADIMMPPFSLLFNETKGLQGLKW
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHH
CIKEDVYMNYGIFLQNILEFVIFSFAIYLILTIFSRTKLEKPKSELKLILESLQKEITLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QEIKNNLQQNNNKK
HHHHHHHHHCCCCC
>Mature Secondary Structure 
TFFQGFKNFITRGNVINLAVAVVIGQLFSKIVSSLVADIMMPPFSLLFNETKGLQGLKW
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHH
CIKEDVYMNYGIFLQNILEFVIFSFAIYLILTIFSRTKLEKPKSELKLILESLQKEITLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QEIKNNLQQNNNKK
HHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA