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The map label for this gene is mraW

Identifier: 197294245

GI number: 197294245

Start: 116012

End: 116923

Strand: Direct

Name: mraW

Synonym: PAa_0107

Alternate gene names: 197294245

Gene position: 116012-116923 (Clockwise)

Preceding gene: 197294244

Following gene: 197294246

Centisome position: 13.18

GC content: 27.3

Gene sequence:

>912_bases
ATGACTTTGAGGCACATTTCAGTTTTAAAAGAAGAAGCTATTTCTTTTTTAAATATTAATCCTAATGGTGTTTATGTTGA
CGCTACTTTAGGTCAGGGAGGACATACTTTATCTATTTTAAATCATCTCCAAGATGGTTTTTTATACTCTTTTGATCAAG
ATTTAAAAGCTTGTTCCGATATTCAAAAGAAATTAAACCCTAATTGGCCTATTGAAATCATTCATAGTAATTTTTCTAAT
CTTAAATTAGAATTAGCCAAAAGAAATGTTTTTCAATTAGATGGTATTTTATTTGATTTAGGGATATCTTCTTGTCAAAT
TGATGATTCTCAAAGAGGTTTTAGTTATTTGCATAATGCTCCTTTGGATATGAGATTTGATAATAGACAAAAACTAACAG
CCCAAGAAATTGTAAATTTTTATTCTTTTGAGAAATTAAAAAACATTTTTCATATTTATGGTGAAGAAAAAAAGGCAGCT
TTGATTGCAAAACAAATTATTAAAAACCGTCCTTTAAAAACATCTTATGATTTAGTGGCTATAACTGATATGTTTTATAA
ATATCATAAAGGGCATAGTGCGAAAAAAATTTTTCAAGCTTTACGAATTGAAGTTAATCAAGAGTTAGAAGTTTTAAAAC
AAGCCTTATCCCAAAGTCTTGAATTATTAAAAAAAGACGGTTCCATTGTTGTTATTAGTTTTCATTCTTTGGAAGATCGT
ATTGTTAAACATTTTTTTAAAAAAAATAGTGTTTTCGAATTTCCTAAAAAATTACCTATTATGAGTGTTGATTATCCTCA
ACCTGCTTTGCGAATTATCACTAAAAAACCTTGTATTCCTAGTGAGGTTGAAATGAAAAATAATCCTCGTAGTATTTCTG
CCAAATTACGTGCAGCTGTTAAAAATATTTAA

Upstream 100 bases:

>100_bases
GTCTTTAAATAAAATAATTAAATAAAACTGTTAAATTACAATGAGAATTGCATAATTTAACTTTTTTTATTTTGTGTAAA
TTGAAAAGGAGCTGTTTACA

Downstream 100 bases:

>100_bases
AAAAATATAGTCTTTTGAAATTAGATTTGCTGAATAAAAAAATAGTTTTTTCCAAACTACTTATGAATGTAGTTTGGTTT
TTTTATTCTTATAAAGAAAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTLRHISVLKEEAISFLNINPNGVYVDATLGQGGHTLSILNHLQDGFLYSFDQDLKACSDIQKKLNPNWPIEIIHSNFSN
LKLELAKRNVFQLDGILFDLGISSCQIDDSQRGFSYLHNAPLDMRFDNRQKLTAQEIVNFYSFEKLKNIFHIYGEEKKAA
LIAKQIIKNRPLKTSYDLVAITDMFYKYHKGHSAKKIFQALRIEVNQELEVLKQALSQSLELLKKDGSIVVISFHSLEDR
IVKHFFKKNSVFEFPKKLPIMSVDYPQPALRIITKKPCIPSEVEMKNNPRSISAKLRAAVKNI

Sequences:

>Translated_303_residues
MTLRHISVLKEEAISFLNINPNGVYVDATLGQGGHTLSILNHLQDGFLYSFDQDLKACSDIQKKLNPNWPIEIIHSNFSN
LKLELAKRNVFQLDGILFDLGISSCQIDDSQRGFSYLHNAPLDMRFDNRQKLTAQEIVNFYSFEKLKNIFHIYGEEKKAA
LIAKQIIKNRPLKTSYDLVAITDMFYKYHKGHSAKKIFQALRIEVNQELEVLKQALSQSLELLKKDGSIVVISFHSLEDR
IVKHFFKKNSVFEFPKKLPIMSVDYPQPALRIITKKPCIPSEVEMKNNPRSISAKLRAAVKNI
>Mature_302_residues
TLRHISVLKEEAISFLNINPNGVYVDATLGQGGHTLSILNHLQDGFLYSFDQDLKACSDIQKKLNPNWPIEIIHSNFSNL
KLELAKRNVFQLDGILFDLGISSCQIDDSQRGFSYLHNAPLDMRFDNRQKLTAQEIVNFYSFEKLKNIFHIYGEEKKAAL
IAKQIIKNRPLKTSYDLVAITDMFYKYHKGHSAKKIFQALRIEVNQELEVLKQALSQSLELLKKDGSIVVISFHSLEDRI
VKHFFKKNSVFEFPKKLPIMSVDYPQPALRIITKKPCIPSEVEMKNNPRSISAKLRAAVKNI

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=338, Percent_Identity=34.6153846153846, Blast_Score=160, Evalue=9e-40,
Organism=Homo sapiens, GI165377202, Length=187, Percent_Identity=35.8288770053476, Blast_Score=105, Evalue=6e-23,
Organism=Escherichia coli, GI1786270, Length=312, Percent_Identity=39.4230769230769, Blast_Score=206, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62472493, Length=336, Percent_Identity=35.4166666666667, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI28571637, Length=336, Percent_Identity=35.4166666666667, Blast_Score=158, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_PHYAS (B1V910)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001798786.1
- ProteinModelPortal:   B1V910
- SMR:   B1V910
- GeneID:   6799540
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- ProtClustDB:   PRK00050
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34763; Mature: 34632

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: BINDING 52-52 BINDING 78-78 BINDING 99-99 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRHISVLKEEAISFLNINPNGVYVDATLGQGGHTLSILNHLQDGFLYSFDQDLKACSD
CCCHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCEECHHHHHHHHHH
IQKKLNPNWPIEIIHSNFSNLKLELAKRNVFQLDGILFDLGISSCQIDDSQRGFSYLHNA
HHHHCCCCCCEEEEECCCCCEEEEHHHCCHHHHHCCCEECCCCCEECCCCHHHHHHHHCC
PLDMRFDNRQKLTAQEIVNFYSFEKLKNIFHIYGEEKKAALIAKQIIKNRPLKTSYDLVA
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHH
ITDMFYKYHKGHSAKKIFQALRIEVNQELEVLKQALSQSLELLKKDGSIVVISFHSLEDR
HHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
IVKHFFKKNSVFEFPKKLPIMSVDYPQPALRIITKKPCIPSEVEMKNNPRSISAKLRAAV
HHHHHHHCCCHHHHHHHCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
KNI
HCC
>Mature Secondary Structure 
TLRHISVLKEEAISFLNINPNGVYVDATLGQGGHTLSILNHLQDGFLYSFDQDLKACSD
CCHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCEECHHHHHHHHHH
IQKKLNPNWPIEIIHSNFSNLKLELAKRNVFQLDGILFDLGISSCQIDDSQRGFSYLHNA
HHHHCCCCCCEEEEECCCCCEEEEHHHCCHHHHHCCCEECCCCCEECCCCHHHHHHHHCC
PLDMRFDNRQKLTAQEIVNFYSFEKLKNIFHIYGEEKKAALIAKQIIKNRPLKTSYDLVA
CCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCHHH
ITDMFYKYHKGHSAKKIFQALRIEVNQELEVLKQALSQSLELLKKDGSIVVISFHSLEDR
HHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHH
IVKHFFKKNSVFEFPKKLPIMSVDYPQPALRIITKKPCIPSEVEMKNNPRSISAKLRAAV
HHHHHHHCCCHHHHHHHCCEEECCCCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
KNI
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA