Click here to switch to the map view.

The map label for this gene is pykA [H]

Identifier: 197294211

GI number: 197294211

Start: 66465

End: 67820

Strand: Direct

Name: pykA [H]

Synonym: PAa_0061

Alternate gene names: 197294211

Gene position: 66465-67820 (Clockwise)

Preceding gene: 197294210

Following gene: 197294221

Centisome position: 7.55

GC content: 30.46

Gene sequence:

>1356_bases
ATGAATAAAACTAAAATAGTGTGCACTTTAGGACCTGCTTGTCGCGACAAAGAAACTTTAAAACAATTAGTAAAAAATGG
TCTTAATGTTGCTCGTTTTAATTTTTCTCACGCTGATTATCAAAACAGCGAAGAAACTTTAAAGATGATTCAAGAAATTA
ACCAAGAATTAAATACTTATGTAGCAACTATGCTAGACACTAAAGGACCAGAAATTAGAACTCATAATTTTGCAAAACCA
GTGGAAATCAAACAAAACTCTGAAGTAAGAATCGCTTTTACAGAAGTTTTAGGAACTGAAAAAAAATTTTCTATCAGTTA
TGAACAACTTTATGATGATGTTACAACTAGTGATTTAATTTTTATTGATGATGGTTATTTAACTTTAGAAGTAGTTGCCA
AAGATGAACAAAACAAAGAATTAATTACAAAAGCTAACAACACTCATTTAGTTAAATCCCGTAGAGGCGTTAATGTTCCC
AACATTAAATTAAAAATGCCTTATATCTCTGAAAAAGATGCTCGCGATATTGCTTTTGCTTGTGAAAAAAAATATGATTT
TTTAGCACTTTCTTTTGTTAGAAATGCTTCAGATGTTTTGGAAGTAAGAAAAATCTTAAAAGAAAAGCAAAACGATACTA
TTAAAATAATCGCTAAAATAGAAAACCAAGAAGGAATAGATAATTTAGAAGAAATTCTTCTTGTTGTTGACGGCATTATG
GTTGCTCGTGGTGATTTAGGAATTGAAGTACCGGGAGAACTTGTTCCTTTATATCAAACTCAAATGATTCAAGAATGTCT
TTGTGCCGGTAAACCTGTTATTGTGGCTACTCAAATGCTTGAATCAATGCAAAGAAACCCTCGCCCTACTAAAGCAGAAA
TTAGTGATGTTTGGAACGCAGTTTTACAAGGCACTACAGCAACTATGTTATCAGGCGAATCGGCATCAGGACTTTATCCT
GAAAAGTCAGTTTCTTATATGGCAAAAATTAATCATAAAGCCGAAGAGTATCTTGATTATGATTTAATTTCTAATTTTTA
TGATCCTCAAAATAGAGTTGAATATCTAGCTTTTAGTGTTATCCAAATGGCTTTAAAAGACCAAATTCATGCAATTGTTA
TTGAAGATGATCTTGCTTATGGTTATGCTTTTGCTAAATTTCGCTCCAAAGTGCCAGTTTTTGTTAAAGTAAAAGATTTA
AAATCAAGTACAACTTTGGCATTAAATTTTTCCAATTTCCCTTTCTTAACTGAGGCGGAATTAATGACTAAATTAAACCT
TTTGAAAAGTGAAAATCAAAATAATTTAGTTTTTGCTGTCATTAAAAAAGATTCCTTAACCTTAAAGAATTTTTAA

Upstream 100 bases:

>100_bases
AACCCCCAGAAATGACAGGAATAAGTTTAATCGAAAAAACAAATAAAACTTCAAAATAAGGATGAAAAAATGAATAAAAC
TTCAAAATAAGGATGAAAAA

Downstream 100 bases:

>100_bases
TTAATTGCTTGCTCACTTTTTATAACTTGTGCTTGCTTTTAGCAAAATTTATAAAAACTTTTTTACTTTTAAAAAAAATT
AGGACCAAGAGGTCTTACGA

Product: Pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNKTKIVCTLGPACRDKETLKQLVKNGLNVARFNFSHADYQNSEETLKMIQEINQELNTYVATMLDTKGPEIRTHNFAKP
VEIKQNSEVRIAFTEVLGTEKKFSISYEQLYDDVTTSDLIFIDDGYLTLEVVAKDEQNKELITKANNTHLVKSRRGVNVP
NIKLKMPYISEKDARDIAFACEKKYDFLALSFVRNASDVLEVRKILKEKQNDTIKIIAKIENQEGIDNLEEILLVVDGIM
VARGDLGIEVPGELVPLYQTQMIQECLCAGKPVIVATQMLESMQRNPRPTKAEISDVWNAVLQGTTATMLSGESASGLYP
EKSVSYMAKINHKAEEYLDYDLISNFYDPQNRVEYLAFSVIQMALKDQIHAIVIEDDLAYGYAFAKFRSKVPVFVKVKDL
KSSTTLALNFSNFPFLTEAELMTKLNLLKSENQNNLVFAVIKKDSLTLKNF

Sequences:

>Translated_451_residues
MNKTKIVCTLGPACRDKETLKQLVKNGLNVARFNFSHADYQNSEETLKMIQEINQELNTYVATMLDTKGPEIRTHNFAKP
VEIKQNSEVRIAFTEVLGTEKKFSISYEQLYDDVTTSDLIFIDDGYLTLEVVAKDEQNKELITKANNTHLVKSRRGVNVP
NIKLKMPYISEKDARDIAFACEKKYDFLALSFVRNASDVLEVRKILKEKQNDTIKIIAKIENQEGIDNLEEILLVVDGIM
VARGDLGIEVPGELVPLYQTQMIQECLCAGKPVIVATQMLESMQRNPRPTKAEISDVWNAVLQGTTATMLSGESASGLYP
EKSVSYMAKINHKAEEYLDYDLISNFYDPQNRVEYLAFSVIQMALKDQIHAIVIEDDLAYGYAFAKFRSKVPVFVKVKDL
KSSTTLALNFSNFPFLTEAELMTKLNLLKSENQNNLVFAVIKKDSLTLKNF
>Mature_451_residues
MNKTKIVCTLGPACRDKETLKQLVKNGLNVARFNFSHADYQNSEETLKMIQEINQELNTYVATMLDTKGPEIRTHNFAKP
VEIKQNSEVRIAFTEVLGTEKKFSISYEQLYDDVTTSDLIFIDDGYLTLEVVAKDEQNKELITKANNTHLVKSRRGVNVP
NIKLKMPYISEKDARDIAFACEKKYDFLALSFVRNASDVLEVRKILKEKQNDTIKIIAKIENQEGIDNLEEILLVVDGIM
VARGDLGIEVPGELVPLYQTQMIQECLCAGKPVIVATQMLESMQRNPRPTKAEISDVWNAVLQGTTATMLSGESASGLYP
EKSVSYMAKINHKAEEYLDYDLISNFYDPQNRVEYLAFSVIQMALKDQIHAIVIEDDLAYGYAFAKFRSKVPVFVKVKDL
KSSTTLALNFSNFPFLTEAELMTKLNLLKSENQNNLVFAVIKKDSLTLKNF

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=435, Percent_Identity=40, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI33286420, Length=435, Percent_Identity=40, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI33286418, Length=438, Percent_Identity=40.1826484018265, Blast_Score=288, Evalue=6e-78,
Organism=Homo sapiens, GI10835121, Length=434, Percent_Identity=38.0184331797235, Blast_Score=272, Evalue=4e-73,
Organism=Homo sapiens, GI32967597, Length=434, Percent_Identity=38.0184331797235, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=43.9446366782007, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=43.9446366782007, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=40.084388185654, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=40.084388185654, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=38.0733944954128, Blast_Score=136, Evalue=4e-32,
Organism=Escherichia coli, GI1787965, Length=345, Percent_Identity=44.0579710144928, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788160, Length=405, Percent_Identity=33.8271604938272, Blast_Score=208, Evalue=7e-55,
Organism=Caenorhabditis elegans, GI17544584, Length=424, Percent_Identity=38.9150943396226, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71984413, Length=345, Percent_Identity=43.1884057971014, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI17506829, Length=345, Percent_Identity=43.1884057971014, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71984406, Length=345, Percent_Identity=43.1884057971014, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17506831, Length=436, Percent_Identity=37.3853211009174, Blast_Score=265, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6319279, Length=441, Percent_Identity=37.18820861678, Blast_Score=262, Evalue=8e-71,
Organism=Saccharomyces cerevisiae, GI6324923, Length=432, Percent_Identity=37.2685185185185, Blast_Score=256, Evalue=4e-69,
Organism=Drosophila melanogaster, GI24648964, Length=353, Percent_Identity=43.9093484419263, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI28571814, Length=353, Percent_Identity=43.9093484419263, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24648966, Length=432, Percent_Identity=34.0277777777778, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24581235, Length=431, Percent_Identity=30.3944315545244, Blast_Score=204, Evalue=8e-53,
Organism=Drosophila melanogaster, GI24646914, Length=320, Percent_Identity=32.1875, Blast_Score=158, Evalue=6e-39,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51077; Mature: 51077

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKTKIVCTLGPACRDKETLKQLVKNGLNVARFNFSHADYQNSEETLKMIQEINQELNTY
CCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VATMLDTKGPEIRTHNFAKPVEIKQNSEVRIAFTEVLGTEKKFSISYEQLYDDVTTSDLI
HHHHHCCCCCCCCCCCCCCCEEECCCCCEEEEEHHHHCCCCEEEEEHHHHHCCCCCCCEE
FIDDGYLTLEVVAKDEQNKELITKANNTHLVKSRRGVNVPNIKLKMPYISEKDARDIAFA
EEECCEEEEEEEECCCCCCHHHEECCCCEEEECCCCCCCCCEEEECCCCCCCCCCHHHHH
CEKKYDFLALSFVRNASDVLEVRKILKEKQNDTIKIIAKIENQEGIDNLEEILLVVDGIM
HHCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
VARGDLGIEVPGELVPLYQTQMIQECLCAGKPVIVATQMLESMQRNPRPTKAEISDVWNA
EECCCCCEECCCCEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VLQGTTATMLSGESASGLYPEKSVSYMAKINHKAEEYLDYDLISNFYDPQNRVEYLAFSV
HHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IQMALKDQIHAIVIEDDLAYGYAFAKFRSKVPVFVKVKDLKSSTTLALNFSNFPFLTEAE
HHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCEEHHH
LMTKLNLLKSENQNNLVFAVIKKDSLTLKNF
HHHHHHHHHCCCCCCEEEEEEECCCEECCCC
>Mature Secondary Structure
MNKTKIVCTLGPACRDKETLKQLVKNGLNVARFNFSHADYQNSEETLKMIQEINQELNTY
CCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
VATMLDTKGPEIRTHNFAKPVEIKQNSEVRIAFTEVLGTEKKFSISYEQLYDDVTTSDLI
HHHHHCCCCCCCCCCCCCCCEEECCCCCEEEEEHHHHCCCCEEEEEHHHHHCCCCCCCEE
FIDDGYLTLEVVAKDEQNKELITKANNTHLVKSRRGVNVPNIKLKMPYISEKDARDIAFA
EEECCEEEEEEEECCCCCCHHHEECCCCEEEECCCCCCCCCEEEECCCCCCCCCCHHHHH
CEKKYDFLALSFVRNASDVLEVRKILKEKQNDTIKIIAKIENQEGIDNLEEILLVVDGIM
HHCCCCHHHHHHHHCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
VARGDLGIEVPGELVPLYQTQMIQECLCAGKPVIVATQMLESMQRNPRPTKAEISDVWNA
EECCCCCEECCCCEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VLQGTTATMLSGESASGLYPEKSVSYMAKINHKAEEYLDYDLISNFYDPQNRVEYLAFSV
HHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
IQMALKDQIHAIVIEDDLAYGYAFAKFRSKVPVFVKVKDLKSSTTLALNFSNFPFLTEAE
HHHHHHCCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCEEHHH
LMTKLNLLKSENQNNLVFAVIKKDSLTLKNF
HHHHHHHHHCCCCCCEEEEEEECCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA