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The map label for this gene is eno

Identifier: 197294209

GI number: 197294209

Start: 62092

End: 63384

Strand: Direct

Name: eno

Synonym: PAa_0059

Alternate gene names: 197294209

Gene position: 62092-63384 (Clockwise)

Preceding gene: 197294208

Following gene: 197294210

Centisome position: 7.06

GC content: 34.11

Gene sequence:

>1293_bases
ATGCCATACATTAAAACAATTAACTCCCTTGAAGTTCTAGATTCAAGAGGAAATCCAACGGTTGAAGTTGAAGTAATTAC
TTTATCAGGAGCTAAAGGAAAAACCTTAGTCCCTTCAGGAGCCTCAACTGGAGAACATGAAGCAGTTGAATTAAGAGATT
CTGATTCTAAAAGATATTTAGGTAAAGGAGTTTTAAAAGCGGTTGAAAATGTTGCAACAGTCATTGAACCTCGTTTACAA
AACTTATCTGTTTTAGACCAAGCCTTAATTGACCAAACTTTAATTCAACTTGACGGAACCCCCAATAAATCTAAATTAGG
AGCTAATGCAATTTTGGGAGTTTCTTTAGCTTGTGCAAGAGCTGCAGCTGATTATTTAGGTTTAGAACTTTATGAATATA
TCGCAGGGATTGCGCCTAAACAAATGCCAGTTCCTATGATGAACGTAATTAATGGTGGGGCTCATGCTTCTAATAGTGTT
GATTTTCAAGAGTTCATGATTTTGCCAACAGGAGCCCCTAGTTTCAAAGAGGCATTGCGTTACGGAGCAGAAGTTTTTCA
TCATTTAGGAAAAATCTTAAAACAAAAAGGATTGCCTACTACAGTAGGAGATGAAGGTGGATACGCTCCTGATCTAAATT
CTAACAAAGAAGCTTTGCAAATCATTTTAGAAGCAATTCAAAATGCGGGTTATGTTCCAGGAAAAGACATTTTTTTAGGG
ATGGACGTTGCCGCTTCTGAATTTTACGACCGCGAAACAAAAAAATATCTTTTAGCGTCTGAAAATAATAAAACTTTTAG
CAGCGAAGAGTTAGTTTCTTATTATGAACAACTAATTAACAAATATCCGATTCTTTCGATCGAAGACGGACTTGACCAAA
ATGATTGGGATGGTTGGAAATTATTAACTCAAAAATTAGGTCAAAAAGTTCAATTAGTTGGAGATGATTTATTTGTGACA
AATACTCAAAAAATACAAGAAGGGATTGACAAACAAATTGCCAATTCAGTTTTAATTAAATTAAATCAAATAGGAACCTT
GACAGAAACTTTAGAAGCGATTGAAATGGCTAAAAAAGCTTCTTACACTGTTGTTATTTCTCATCGTAGTGGCGAAACAG
AAGATACTACCATTGCTGATTTAGCAGTAGCAATGAACACAGGTCAAATTAAGACTGGTTCTTGTTCTCGTACGGACCGT
ATTGCTAAATACAATCAGTTATTAAGAATTGAAAAAAATATGTCTAATCCATCTTATTTAGGTCTTAAAGTTTTTTACAA
TTTAAAAAAATAA

Upstream 100 bases:

>100_bases
ACATTAAAATATCTTTTATGATAAAAAAGTATTATAATATTTATTGGTTATCAAATATTTAATTTTCAATATTTTACAAT
TAAATTAAAAAGGAGAATTT

Downstream 100 bases:

>100_bases
AATGAATTCAAAATATTAACAACATAAAAAAAATAGCATCTACATTGTGAATATGACTGAAAAATTTTTGATTTTTTAAA
TCTTTTCCTTTAGAAGTGCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MPYIKTINSLEVLDSRGNPTVEVEVITLSGAKGKTLVPSGASTGEHEAVELRDSDSKRYLGKGVLKAVENVATVIEPRLQ
NLSVLDQALIDQTLIQLDGTPNKSKLGANAILGVSLACARAAADYLGLELYEYIAGIAPKQMPVPMMNVINGGAHASNSV
DFQEFMILPTGAPSFKEALRYGAEVFHHLGKILKQKGLPTTVGDEGGYAPDLNSNKEALQIILEAIQNAGYVPGKDIFLG
MDVAASEFYDRETKKYLLASENNKTFSSEELVSYYEQLINKYPILSIEDGLDQNDWDGWKLLTQKLGQKVQLVGDDLFVT
NTQKIQEGIDKQIANSVLIKLNQIGTLTETLEAIEMAKKASYTVVISHRSGETEDTTIADLAVAMNTGQIKTGSCSRTDR
IAKYNQLLRIEKNMSNPSYLGLKVFYNLKK

Sequences:

>Translated_430_residues
MPYIKTINSLEVLDSRGNPTVEVEVITLSGAKGKTLVPSGASTGEHEAVELRDSDSKRYLGKGVLKAVENVATVIEPRLQ
NLSVLDQALIDQTLIQLDGTPNKSKLGANAILGVSLACARAAADYLGLELYEYIAGIAPKQMPVPMMNVINGGAHASNSV
DFQEFMILPTGAPSFKEALRYGAEVFHHLGKILKQKGLPTTVGDEGGYAPDLNSNKEALQIILEAIQNAGYVPGKDIFLG
MDVAASEFYDRETKKYLLASENNKTFSSEELVSYYEQLINKYPILSIEDGLDQNDWDGWKLLTQKLGQKVQLVGDDLFVT
NTQKIQEGIDKQIANSVLIKLNQIGTLTETLEAIEMAKKASYTVVISHRSGETEDTTIADLAVAMNTGQIKTGSCSRTDR
IAKYNQLLRIEKNMSNPSYLGLKVFYNLKK
>Mature_429_residues
PYIKTINSLEVLDSRGNPTVEVEVITLSGAKGKTLVPSGASTGEHEAVELRDSDSKRYLGKGVLKAVENVATVIEPRLQN
LSVLDQALIDQTLIQLDGTPNKSKLGANAILGVSLACARAAADYLGLELYEYIAGIAPKQMPVPMMNVINGGAHASNSVD
FQEFMILPTGAPSFKEALRYGAEVFHHLGKILKQKGLPTTVGDEGGYAPDLNSNKEALQIILEAIQNAGYVPGKDIFLGM
DVAASEFYDRETKKYLLASENNKTFSSEELVSYYEQLINKYPILSIEDGLDQNDWDGWKLLTQKLGQKVQLVGDDLFVTN
TQKIQEGIDKQIANSVLIKLNQIGTLTETLEAIEMAKKASYTVVISHRSGETEDTTIADLAVAMNTGQIKTGSCSRTDRI
AKYNQLLRIEKNMSNPSYLGLKVFYNLKK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=51.0344827586207, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=51.0344827586207, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI5803011, Length=436, Percent_Identity=49.5412844036697, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=49.1954022988506, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=45.7274826789838, Blast_Score=358, Evalue=5e-99,
Organism=Homo sapiens, GI169201331, Length=346, Percent_Identity=26.5895953757225, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI169201757, Length=346, Percent_Identity=26.5895953757225, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI239744207, Length=346, Percent_Identity=26.5895953757225, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1789141, Length=434, Percent_Identity=61.9815668202765, Blast_Score=502, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI71995829, Length=426, Percent_Identity=53.2863849765258, Blast_Score=416, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=426, Percent_Identity=53.2863849765258, Blast_Score=416, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI32563855, Length=196, Percent_Identity=48.9795918367347, Blast_Score=190, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324974, Length=441, Percent_Identity=48.9795918367347, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6324969, Length=441, Percent_Identity=48.9795918367347, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=441, Percent_Identity=48.7528344671202, Blast_Score=391, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6321693, Length=438, Percent_Identity=47.4885844748858, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=438, Percent_Identity=46.8036529680365, Blast_Score=357, Evalue=2e-99,
Organism=Drosophila melanogaster, GI24580918, Length=423, Percent_Identity=52.9550827423168, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=423, Percent_Identity=52.9550827423168, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=423, Percent_Identity=52.9550827423168, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=423, Percent_Identity=52.9550827423168, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=423, Percent_Identity=52.9550827423168, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=423, Percent_Identity=52.9550827423168, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_PHYAS (B1V8R6)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001798750.1
- ProteinModelPortal:   B1V8R6
- SMR:   B1V8R6
- GeneID:   6798964
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46998; Mature: 46867

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 340-340 BINDING 155-155 BINDING 164-164 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYIKTINSLEVLDSRGNPTVEVEVITLSGAKGKTLVPSGASTGEHEAVELRDSDSKRYL
CCCHHCCCHHHHHCCCCCCEEEEEEEEEECCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVATVIEPRLQNLSVLDQALIDQTLIQLDGTPNKSKLGANAILGVSLACAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAADYLGLELYEYIAGIAPKQMPVPMMNVINGGAHASNSVDFQEFMILPTGAPSFKEALR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHH
YGAEVFHHLGKILKQKGLPTTVGDEGGYAPDLNSNKEALQIILEAIQNAGYVPGKDIFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDVAASEFYDRETKKYLLASENNKTFSSEELVSYYEQLINKYPILSIEDGLDQNDWDGWK
CCHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHH
LLTQKLGQKVQLVGDDLFVTNTQKIQEGIDKQIANSVLIKLNQIGTLTETLEAIEMAKKA
HHHHHCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
SYTVVISHRSGETEDTTIADLAVAMNTGQIKTGSCSRTDRIAKYNQLLRIEKNMSNPSYL
CEEEEEEECCCCCCCCHHHHHHEEECCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCEE
GLKVFYNLKK
EEEEEEEECC
>Mature Secondary Structure 
PYIKTINSLEVLDSRGNPTVEVEVITLSGAKGKTLVPSGASTGEHEAVELRDSDSKRYL
CCHHCCCHHHHHCCCCCCEEEEEEEEEECCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVLKAVENVATVIEPRLQNLSVLDQALIDQTLIQLDGTPNKSKLGANAILGVSLACAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHH
AAADYLGLELYEYIAGIAPKQMPVPMMNVINGGAHASNSVDFQEFMILPTGAPSFKEALR
HHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEECCCCHHHHHHHH
YGAEVFHHLGKILKQKGLPTTVGDEGGYAPDLNSNKEALQIILEAIQNAGYVPGKDIFLG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEE
MDVAASEFYDRETKKYLLASENNKTFSSEELVSYYEQLINKYPILSIEDGLDQNDWDGWK
CCHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCHHHH
LLTQKLGQKVQLVGDDLFVTNTQKIQEGIDKQIANSVLIKLNQIGTLTETLEAIEMAKKA
HHHHHCCCEEEEECCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
SYTVVISHRSGETEDTTIADLAVAMNTGQIKTGSCSRTDRIAKYNQLLRIEKNMSNPSYL
CEEEEEEECCCCCCCCHHHHHHEEECCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCEE
GLKVFYNLKK
EEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA