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The map label for this gene is rnjA [H]

Identifier: 197294179

GI number: 197294179

Start: 13226

End: 14914

Strand: Direct

Name: rnjA [H]

Synonym: PAa_0011

Alternate gene names: 197294179

Gene position: 13226-14914 (Clockwise)

Preceding gene: 197294175

Following gene: 197294180

Centisome position: 1.5

GC content: 29.6

Gene sequence:

>1689_bases
ATGAATAATGTGACCTCAAATATAGACTTAAATAAGTTGGATGTTTCTTTCTTTGCCCTAGGAGGTCTGGGCGAAGTTGG
CAAAAATATGTATGTTTTTGAAATAGGAAAAGAAATTTTTATTGTTGATTCAGGTTTTTCTTTTTCAGAAAATTTTTTAT
TAGGAATTAATTATGTTATTCCTGATTATCAGTATCTAAGAGATAACGAATCAAGGATTGTAGGCCTTTTTATCACTCAC
GGTCATGAAGACCATATCGGCGGAATCCCTTATTTATTAAAAAAAGTTCATATTCCTAAAATTTTTGTTTCAGGGATTGC
TTATCATTTATTAAGATACAAATTATTAGAACATAAAGATGTTGGTGCACTGCCACCAATGGTTAATTATAACGCTGATT
CTTGTTATCATTTTCAAAATGCTTCAATTTCTTTTATTAGAGTTAATCATTCTATTCCTGATACTTTTGGTATTGTTATC
AAAACTAAATATGGCAATCTTTTTTATACTAGTGATTTTAAAATTGATTATACTCCAGTAGGACCTCCGGCTGAATATGA
GAAATTAATTAAAATCCGACAAGAAGGGGTTTTATGTTTACTTTCTGATTCTACCAATGCTGAACAAGAAGGAGTAGTTA
AATCGGAAAGTACTATCGGACATTCTATTGATGAATTGTTTGTTCAAATACCTGATAGAATTATTATAGTTACTTTTGCT
TCTAATTTTTATCGGATCAAGCAAATTGTTGAAGCCACTATTTTAACTAAAAGAAAAATTGCTGTTTTTGGCCGTAGTAT
GAAAAAATCTTTAGAAATTGGTCAAAAAAACGGTTATTTAAATATTCCTGAAGGAACTTTGGTCCCTAGTCATAATATTC
ATAAATATCGTGATATTATTTTATTATGTACAGGTTCTCAAGGTGAGCCTCTTGCGGCTTTAAGTCGTATGGCAAACAAT
AGTCACAGACAAATTAAACTTAATTATAAAGACACTATTATTTTTTCTTCTTCTCCTATTCCTGGTAACCAAGAAAACAT
TAATAAAACTCTTGACCTTCTTTTACAAAAAAATGTTAATGTTATTACTCACGGACCTTTAATTAACACTCACGTTTCGG
GTCATGGAAATCAAAATGACCTTAAATTAATGCTTACCTTAATTCAACCTAAATATTTTATTCCGGTGCATGGTGAACGT
CGTATGTTGCAGGCTCACAAAAATTTAGCGGTAGAATGCAACATTTCAGAAGAAAATATTTTTATTTTAGATAACGGCCA
AAGAATTTCTTTAATTCCTGATGAAGCCCATATTGTAAATACTATTCCTATGGAAGATGTTTTTCTTGACGCTTCGGGCA
TGAATTATCTTGGTAATTCTATTTTGCAAGAACGTCGTTTTTTAAGTCAAGAAGGTTTGTTTGCGGTTGTTATGAGTATA
GACGTTAAAAAAAAGCAAATCCTTAGTTCGCCAACAATTGTTTCTCGTGGATTTATTTATATGAAAAGCAACCAAGAGTT
AATTAAAAAAATTTCTTTGGATATTAAAAATAGCATTGAAAAAGCTATTAAAAACAATGAAGTAGACAAAGAAGTTTTAA
AGAAAATCATCATTGATTATCTTTCTACCAAAATATATCAAATGACTTTTAGAAAGCCTATTATTATTCCTATTATTTTA
ACTATATAA

Upstream 100 bases:

>100_bases
AAATTACAATTATTTGTTTATATTATACTATAATATAAGTAGTAATAATCGTTAAAATACTATTTTAATATAGTTATTTT
GAAAAAAAGGGAGATTTTTT

Downstream 100 bases:

>100_bases
TAGAACCATTTTGTTTAATTCAAAATTAAAAAATCTAATTATTTTTTTATCTAACTTAATACCATTTTTTTAACAATAAA
TAAATATTTTTGTAAAAAAG

Product: Metallo-beta-lactamase family protein

Products: NA

Alternate protein names: RNase J1 [H]

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MNNVTSNIDLNKLDVSFFALGGLGEVGKNMYVFEIGKEIFIVDSGFSFSENFLLGINYVIPDYQYLRDNESRIVGLFITH
GHEDHIGGIPYLLKKVHIPKIFVSGIAYHLLRYKLLEHKDVGALPPMVNYNADSCYHFQNASISFIRVNHSIPDTFGIVI
KTKYGNLFYTSDFKIDYTPVGPPAEYEKLIKIRQEGVLCLLSDSTNAEQEGVVKSESTIGHSIDELFVQIPDRIIIVTFA
SNFYRIKQIVEATILTKRKIAVFGRSMKKSLEIGQKNGYLNIPEGTLVPSHNIHKYRDIILLCTGSQGEPLAALSRMANN
SHRQIKLNYKDTIIFSSSPIPGNQENINKTLDLLLQKNVNVITHGPLINTHVSGHGNQNDLKLMLTLIQPKYFIPVHGER
RMLQAHKNLAVECNISEENIFILDNGQRISLIPDEAHIVNTIPMEDVFLDASGMNYLGNSILQERRFLSQEGLFAVVMSI
DVKKKQILSSPTIVSRGFIYMKSNQELIKKISLDIKNSIEKAIKNNEVDKEVLKKIIIDYLSTKIYQMTFRKPIIIPIIL
TI

Sequences:

>Translated_562_residues
MNNVTSNIDLNKLDVSFFALGGLGEVGKNMYVFEIGKEIFIVDSGFSFSENFLLGINYVIPDYQYLRDNESRIVGLFITH
GHEDHIGGIPYLLKKVHIPKIFVSGIAYHLLRYKLLEHKDVGALPPMVNYNADSCYHFQNASISFIRVNHSIPDTFGIVI
KTKYGNLFYTSDFKIDYTPVGPPAEYEKLIKIRQEGVLCLLSDSTNAEQEGVVKSESTIGHSIDELFVQIPDRIIIVTFA
SNFYRIKQIVEATILTKRKIAVFGRSMKKSLEIGQKNGYLNIPEGTLVPSHNIHKYRDIILLCTGSQGEPLAALSRMANN
SHRQIKLNYKDTIIFSSSPIPGNQENINKTLDLLLQKNVNVITHGPLINTHVSGHGNQNDLKLMLTLIQPKYFIPVHGER
RMLQAHKNLAVECNISEENIFILDNGQRISLIPDEAHIVNTIPMEDVFLDASGMNYLGNSILQERRFLSQEGLFAVVMSI
DVKKKQILSSPTIVSRGFIYMKSNQELIKKISLDIKNSIEKAIKNNEVDKEVLKKIIIDYLSTKIYQMTFRKPIIIPIIL
TI
>Mature_562_residues
MNNVTSNIDLNKLDVSFFALGGLGEVGKNMYVFEIGKEIFIVDSGFSFSENFLLGINYVIPDYQYLRDNESRIVGLFITH
GHEDHIGGIPYLLKKVHIPKIFVSGIAYHLLRYKLLEHKDVGALPPMVNYNADSCYHFQNASISFIRVNHSIPDTFGIVI
KTKYGNLFYTSDFKIDYTPVGPPAEYEKLIKIRQEGVLCLLSDSTNAEQEGVVKSESTIGHSIDELFVQIPDRIIIVTFA
SNFYRIKQIVEATILTKRKIAVFGRSMKKSLEIGQKNGYLNIPEGTLVPSHNIHKYRDIILLCTGSQGEPLAALSRMANN
SHRQIKLNYKDTIIFSSSPIPGNQENINKTLDLLLQKNVNVITHGPLINTHVSGHGNQNDLKLMLTLIQPKYFIPVHGER
RMLQAHKNLAVECNISEENIFILDNGQRISLIPDEAHIVNTIPMEDVFLDASGMNYLGNSILQERRFLSQEGLFAVVMSI
DVKKKQILSSPTIVSRGFIYMKSNQELIKKISLDIKNSIEKAIKNNEVDKEVLKKIIIDYLSTKIYQMTFRKPIIIPIIL
TI

Specific function: Cleaves the 5'-leader sequence of certain mRNAs. May play a role in maturation and stability of specific mRNAs. Is also required for the maturation of the 16S rRNA. Acts preferentially on 16S rRNA precursors after association of the 30S and 50S ribosomal

COG id: COG0595

COG function: function code R; Predicted hydrolase of the metallo-beta-lactamase superfamily

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. RNase J subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR011108
- InterPro:   IPR004613
- InterPro:   IPR001587 [H]

Pfam domain/function: PF00753 Lactamase_B; PF07521 RMMBL [H]

EC number: NA

Molecular weight: Translated: 63568; Mature: 63568

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01292 UPF0036

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVTSNIDLNKLDVSFFALGGLGEVGKNMYVFEIGKEIFIVDSGFSFSENFLLGINYVI
CCCCCCCCCCEEEEEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCEEEEEEEEC
PDYQYLRDNESRIVGLFITHGHEDHIGGIPYLLKKVHIPKIFVSGIAYHLLRYKLLEHKD
CCHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
VGALPPMVNYNADSCYHFQNASISFIRVNHSIPDTFGIVIKTKYGNLFYTSDFKIDYTPV
CCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCEEEEEEEECCCEEEEECCEEEEECC
GPPAEYEKLIKIRQEGVLCLLSDSTNAEQEGVVKSESTIGHSIDELFVQIPDRIIIVTFA
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHCCCEEEEEEEC
SNFYRIKQIVEATILTKRKIAVFGRSMKKSLEIGQKNGYLNIPEGTLVPSHNIHKYRDII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEE
LLCTGSQGEPLAALSRMANNSHRQIKLNYKDTIIFSSSPIPGNQENINKTLDLLLQKNVN
EEEECCCCCHHHHHHHHHCCCCCEEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
VITHGPLINTHVSGHGNQNDLKLMLTLIQPKYFIPVHGERRMLQAHKNLAVECNISEENI
EEECCCEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHCCCEEEEEEECCCEE
FILDNGQRISLIPDEAHIVNTIPMEDVFLDASGMNYLGNSILQERRFLSQEGLFAVVMSI
EEEECCCEEEEECCCCEEEEECCHHHEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEE
DVKKKQILSSPTIVSRGFIYMKSNQELIKKISLDIKNSIEKAIKNNEVDKEVLKKIIIDY
CCHHHHHHCCCCEEECCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LSTKIYQMTFRKPIIIPIILTI
HHHHHEEHHCCCCEEEEEEEEC
>Mature Secondary Structure
MNNVTSNIDLNKLDVSFFALGGLGEVGKNMYVFEIGKEIFIVDSGFSFSENFLLGINYVI
CCCCCCCCCCEEEEEEEEEECCCHHHCCCEEEEEECCEEEEEECCCCCCCCEEEEEEEEC
PDYQYLRDNESRIVGLFITHGHEDHIGGIPYLLKKVHIPKIFVSGIAYHLLRYKLLEHKD
CCHHHHCCCCCEEEEEEEECCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCC
VGALPPMVNYNADSCYHFQNASISFIRVNHSIPDTFGIVIKTKYGNLFYTSDFKIDYTPV
CCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCEEEEEEEECCCEEEEECCEEEEECC
GPPAEYEKLIKIRQEGVLCLLSDSTNAEQEGVVKSESTIGHSIDELFVQIPDRIIIVTFA
CCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCHHCCCHHHHHHHCCCEEEEEEEC
SNFYRIKQIVEATILTKRKIAVFGRSMKKSLEIGQKNGYLNIPEGTLVPSHNIHKYRDII
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCEEEEE
LLCTGSQGEPLAALSRMANNSHRQIKLNYKDTIIFSSSPIPGNQENINKTLDLLLQKNVN
EEEECCCCCHHHHHHHHHCCCCCEEEEEECEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
VITHGPLINTHVSGHGNQNDLKLMLTLIQPKYFIPVHGERRMLQAHKNLAVECNISEENI
EEECCCEEEEEECCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHCCCEEEEEEECCCEE
FILDNGQRISLIPDEAHIVNTIPMEDVFLDASGMNYLGNSILQERRFLSQEGLFAVVMSI
EEEECCCEEEEECCCCEEEEECCHHHEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEEEE
DVKKKQILSSPTIVSRGFIYMKSNQELIKKISLDIKNSIEKAIKNNEVDKEVLKKIIIDY
CCHHHHHHCCCCEEECCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
LSTKIYQMTFRKPIIIPIILTI
HHHHHEEHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969500; 9384377 [H]