| Definition | Clostridium botulinum B1 str. Okra, complete genome. |
|---|---|
| Accession | NC_010516 |
| Length | 3,958,233 |
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The map label for this gene is 170757810
Identifier: 170757810
GI number: 170757810
Start: 887795
End: 889009
Strand: Direct
Name: 170757810
Synonym: CLD_3794
Alternate gene names: NA
Gene position: 887795-889009 (Clockwise)
Preceding gene: 170756107
Following gene: 170754922
Centisome position: 22.43
GC content: 28.4
Gene sequence:
>1215_bases ATGAAATTGAAAAATTTATTTAAAAGAAGCTTATCTCTTTTATTTTCATTTATAATGATTTTCACATTGGTTTCAGGCTT AAATGTAAAGGCATTTTTAGGGGATGAACCCAAACAAACTCTTAATGTGGCTTTATATGAATATGTTCCTGATCCTATAC GTTTTAAAAAAGCAGTTGAAACTGAGTGGAATAAGAAAGAACCTAATATAAGACTTAATTTTGTAGATTGGGATTGTTAT AGTGAGGATCCACCAAAAGATTTAGATGTATTTGTTTTTGATGCAGTTTATTTATCTCATTTCGTAAAGGAAGGATATTT ATCAGAAATTCCAGAAAAGGATATAAAAAATAAAGAAGATATTCTACCTTTCGCAATGGAAGGATGTACTATAAAAGGTT CAGCTTATGCAATTCCTCAAATAATATGTACTAATCTCTTATTTAGTAGAAAAGGAGATTATGACATACAAAAAGTAAAT TCTGTGTATGATTTATATGACAAGTTAGGAAAGTTCACATCAGAAGATATTATTCTTCCTAACAATAAAGGACTGTTAAT AGATATGTCTGGAGGAACTAGTAAAGCTTGTATGTATCTAGATTCACTAATTGATACAACTCAAGAGTACACCAAATTTT GTTCATTACCCAATTTAAATGAATTAAATAAAGATGCAATTGAAAGTTTAGTATTGTTACAATCAATGGCTGGAAAAAGT CAGGCTAATTACTGGCCAGAAAATAATGATTCATATATTCGAGCTAAATGGTTTATTAACGGAAAAGGGAGAGCTTATAT AGGTTATACTGAAGCAATGTCCCAAATGAAAGAATTTGCAAATGATATTGATTTTAAGACAATTTCTTTATCAAAAAATT CTAATATTCCTATTTTTTATGGAGATGTGGTTGGAATAAACTCTTCTATAACGAATTCATATAAGAAAGAAAAAGCAATT GAGCTTGCTAATATTATTACAGACAAAAACACTATGGTGAAAGCAGTTTCTCCAGATGAAAATAATAAATACCCTCAATA TTTATTACCTGCTAGAAGAAGTGTATATCATGATTTAGAAAATAAGTATCCTATTTATGGTAGATTATATAAAATAGCTG ATGATTCTAATAATAAGCTGTTTAGGACTGGCCCTGAAATACGTGAATGGTTAAAACAAGCAAAGAAAATTATTACTGAA TATTTACAACAATGA
Upstream 100 bases:
>100_bases TTGGGGGAGCGAAAAGAACAAACCACCCTTTATTATATTTGATGTAGAAAAATAACTGAATTTAGAGATACATTACATGC ATAATGAAAGGAGTAAACCC
Downstream 100 bases:
>100_bases TATAATAAATAATAATCATTGAAATTAAAGGTACTTTTATTATTAAAAGTGCCTTTTTGTAGAAGGGAGAAGAGTTTATG AAGAAAGTACTTAGTATTGC
Product: putative thiaminase I
Products: NA
Alternate protein names: Thiaminase I; Thiamine pyridinylase [H]
Number of amino acids: Translated: 404; Mature: 404
Protein sequence:
>404_residues MKLKNLFKRSLSLLFSFIMIFTLVSGLNVKAFLGDEPKQTLNVALYEYVPDPIRFKKAVETEWNKKEPNIRLNFVDWDCY SEDPPKDLDVFVFDAVYLSHFVKEGYLSEIPEKDIKNKEDILPFAMEGCTIKGSAYAIPQIICTNLLFSRKGDYDIQKVN SVYDLYDKLGKFTSEDIILPNNKGLLIDMSGGTSKACMYLDSLIDTTQEYTKFCSLPNLNELNKDAIESLVLLQSMAGKS QANYWPENNDSYIRAKWFINGKGRAYIGYTEAMSQMKEFANDIDFKTISLSKNSNIPIFYGDVVGINSSITNSYKKEKAI ELANIITDKNTMVKAVSPDENNKYPQYLLPARRSVYHDLENKYPIYGRLYKIADDSNNKLFRTGPEIREWLKQAKKIITE YLQQ
Sequences:
>Translated_404_residues MKLKNLFKRSLSLLFSFIMIFTLVSGLNVKAFLGDEPKQTLNVALYEYVPDPIRFKKAVETEWNKKEPNIRLNFVDWDCY SEDPPKDLDVFVFDAVYLSHFVKEGYLSEIPEKDIKNKEDILPFAMEGCTIKGSAYAIPQIICTNLLFSRKGDYDIQKVN SVYDLYDKLGKFTSEDIILPNNKGLLIDMSGGTSKACMYLDSLIDTTQEYTKFCSLPNLNELNKDAIESLVLLQSMAGKS QANYWPENNDSYIRAKWFINGKGRAYIGYTEAMSQMKEFANDIDFKTISLSKNSNIPIFYGDVVGINSSITNSYKKEKAI ELANIITDKNTMVKAVSPDENNKYPQYLLPARRSVYHDLENKYPIYGRLYKIADDSNNKLFRTGPEIREWLKQAKKIITE YLQQ >Mature_404_residues MKLKNLFKRSLSLLFSFIMIFTLVSGLNVKAFLGDEPKQTLNVALYEYVPDPIRFKKAVETEWNKKEPNIRLNFVDWDCY SEDPPKDLDVFVFDAVYLSHFVKEGYLSEIPEKDIKNKEDILPFAMEGCTIKGSAYAIPQIICTNLLFSRKGDYDIQKVN SVYDLYDKLGKFTSEDIILPNNKGLLIDMSGGTSKACMYLDSLIDTTQEYTKFCSLPNLNELNKDAIESLVLLQSMAGKS QANYWPENNDSYIRAKWFINGKGRAYIGYTEAMSQMKEFANDIDFKTISLSKNSNIPIFYGDVVGINSSITNSYKKEKAI ELANIITDKNTMVKAVSPDENNKYPQYLLPARRSVYHDLENKYPIYGRLYKIADDSNNKLFRTGPEIREWLKQAKKIITE YLQQ
Specific function: Degrades thiamine by replacing its thiazole moiety with a wide range of nucleophiles [H]
COG id: NA
COG function: NA
Gene ontology:
Cell location: Secreted [H]
Metaboloic importance: NA
Operon status: Not Known
Operon components: None
Similarity: NA
Homologues:
None
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR006059 [H]
Pfam domain/function: PF01547 SBP_bac_1 [H]
EC number: =2.5.1.2 [H]
Molecular weight: Translated: 46446; Mature: 46446
Theoretical pI: Translated: 6.57; Mature: 6.57
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.2 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.5 %Cys+Met (Translated Protein) 1.2 %Cys (Mature Protein) 2.2 %Met (Mature Protein) 3.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKLKNLFKRSLSLLFSFIMIFTLVSGLNVKAFLGDEPKQTLNVALYEYVPDPIRFKKAVE CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHH TEWNKKEPNIRLNFVDWDCYSEDPPKDLDVFVFDAVYLSHFVKEGYLSEIPEKDIKNKED CCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC ILPFAMEGCTIKGSAYAIPQIICTNLLFSRKGDYDIQKVNSVYDLYDKLGKFTSEDIILP CCCCHHCCCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE NNKGLLIDMSGGTSKACMYLDSLIDTTQEYTKFCSLPNLNELNKDAIESLVLLQSMAGKS CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC QANYWPENNDSYIRAKWFINGKGRAYIGYTEAMSQMKEFANDIDFKTISLSKNSNIPIFY CCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEE GDVVGINSSITNSYKKEKAIELANIITDKNTMVKAVSPDENNKYPQYLLPARRSVYHDLE CCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHC NKYPIYGRLYKIADDSNNKLFRTGPEIREWLKQAKKIITEYLQQ CCCCHHEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure MKLKNLFKRSLSLLFSFIMIFTLVSGLNVKAFLGDEPKQTLNVALYEYVPDPIRFKKAVE CCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHH TEWNKKEPNIRLNFVDWDCYSEDPPKDLDVFVFDAVYLSHFVKEGYLSEIPEKDIKNKED CCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC ILPFAMEGCTIKGSAYAIPQIICTNLLFSRKGDYDIQKVNSVYDLYDKLGKFTSEDIILP CCCCHHCCCEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE NNKGLLIDMSGGTSKACMYLDSLIDTTQEYTKFCSLPNLNELNKDAIESLVLLQSMAGKS CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCC QANYWPENNDSYIRAKWFINGKGRAYIGYTEAMSQMKEFANDIDFKTISLSKNSNIPIFY CCCCCCCCCCCEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEE GDVVGINSSITNSYKKEKAIELANIITDKNTMVKAVSPDENNKYPQYLLPARRSVYHDLE CCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHC NKYPIYGRLYKIADDSNNKLFRTGPEIREWLKQAKKIITEYLQQ CCCCHHEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8631946; 9843405 [H]