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The map label for this gene is mtbP [H]

Identifier: 170757755

GI number: 170757755

Start: 2334618

End: 2336303

Strand: Reverse

Name: mtbP [H]

Synonym: CLD_2435

Alternate gene names: 170757755

Gene position: 2336303-2334618 (Counterclockwise)

Preceding gene: 170754282

Following gene: 170756489

Centisome position: 59.02

GC content: 30.25

Gene sequence:

>1686_bases
ATGTTAAAAGTAAGAACATTATTTAGTGGAATAGGTTCACCTGAAAGAGCTTTAAAAGATTTACAAATACCATATGAGTT
AGTTGATTTTTGTGAAGTAGACAAATATGCAGTGAAAAGTTACTGTTCAGTTCATGGAGTTTCAGAGGAAAAGAATCTAG
GTGATATTACAAAAGTTTGGGGAAGAAACCTTCCTTATGCAGATTTATTAGTTTGGGGGTTCCCATGCCCAGACATTAGT
GTAGCAGGTAAACAGAAAGGAATAAAAGAAGGAGAAACTAGGAGTGGATTATATTACGAAGGATTTAGGATATTAAAAGA
AACTAGACCTAAATATTCAATTATAGAAAATGTTAAAAACTTAGTAGGAAAGAGGTTTAAAGCTGATTTTGAAAGTATGC
TTGAGGATATAGAAAGTTTAGGATATAACAACTATTGGAAAGTACTCAATGCTAAAGATTATGGGATACCTCAAAACAGA
GAACGAGTGTTTATAGTGAGTATTAGAAAAGATATTGATACTGGGATCTTCAGTTTCCCTAAAGGATTTGACAATGGATT
AAGACTTAAAGACTTTTTAGAGAATGAAGTTGATGAAAAGTATTATATAAGTGAAAAAGCCACTCAAGGGCTATTAAGGG
CATTAAAAAGACCACATACACCTAAAATGCTTAATAAAGATTCGGAGTATTCACCAACATTAGATACAAGAGTTGGAGCG
TTAACACATAGAAGTCCTTATATGTGCATTCCATGCATAACACCAGATAGGGCGGAAAAGCGTCAGAATGGCAGAAGATT
TAAAGAAAATGGAGATCCTATGTTTACTCTAACAAGTCAAGATAGACATGGAATCTTACAAGTTGGAAACATAGTTGACA
CAGGTAATTGGGGTAATCCGCAAAGAGGTAGAATTTATTCTCCGGATGGAATAAGTCCAGCACTAAATACCGTTGGTGGC
GGAGGATTAGAACCTAAGATATTGAAATACAAAAGAACTGAGTACAGAATTCGCAAATTAACTCCAAAAGAATGTTGGAG
ATTGATGGGTTTTACAGATGAAGATTTTCAAAAGGCTAAAGATGAAATTAGACCAATACTCAATGGCATTTGTATAAATA
AAAATGAGTTTATAGCCTTAGATGGATATAGATTAGCACTAAGAAAAGGAAATTTTGAAACTAAAGAACCGGTTATTGTA
GCAGCAGATTTAATAAAAGTAATTAACAAAGTTAAATATGAATGTAATGTTAAAATTTACTACAATGATAATTATGTTAA
ATTTAAGTTTGACGATTTGGAAATAGTTGGTTATAGACGACAAGGCAATTATATAAATTATAATTCTATAATTCCAGAGA
ATTATAACACTAAAGTTACATTAAAAATTAAACCTATACTTGATATTTTAAAAGATTATAAAAAGAATAAGTTTAAAGTT
GTAGATTTAGATTTTCAAAATGACAAGTTAATTATTAAAGCTAATAACGATATTGCAACAGTAGAAGAAAGTATAACTAT
AGAGTTACAAGGTGAACCTTTAGAAATTTCATTTAATGCAGATTATTTAATAGATGCATTTAAGAATTATGACAATGCAA
CTTTAGAATTAACATATTGTGTAAATCCAATGGTAATAAGAGAAAATAATAAATTAGATTTAGTGCTACCAGTTAGAAAG
AAATAG

Upstream 100 bases:

>100_bases
TAATTTGAAATTATTGTGTACTAACTGAGATTAAAAAATAAATAGGAGTAATTCTAGATAAAAAGTGTGGCTACACCTCC
TGACGAGGAGGAAGTATTAA

Downstream 100 bases:

>100_bases
GGAGCTTAAAAGGCAGGTGAGAAACAGTGTTTAAAGGATACATTCCCACAGGTGGTAAAGATGGTAAAAGACCAACAGAA
GAATATAAAGATAGAACAGA

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.Phi3TI; Cytosine-specific methyltransferase Phi3TI [H]

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVWGRNLPYADLLVWGFPCPDIS
VAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKNLVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNR
ERVFIVSIRKDIDTGIFSFPKGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA
LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNPQRGRIYSPDGISPALNTVGG
GGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAKDEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIV
AADLIKVINKVKYECNVKIYYNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV
VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYCVNPMVIRENNKLDLVLPVRK
K

Sequences:

>Translated_561_residues
MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVWGRNLPYADLLVWGFPCPDIS
VAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKNLVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNR
ERVFIVSIRKDIDTGIFSFPKGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA
LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNPQRGRIYSPDGISPALNTVGG
GGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAKDEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIV
AADLIKVINKVKYECNVKIYYNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV
VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYCVNPMVIRENNKLDLVLPVRK
K
>Mature_561_residues
MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVWGRNLPYADLLVWGFPCPDIS
VAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKNLVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNR
ERVFIVSIRKDIDTGIFSFPKGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA
LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNPQRGRIYSPDGISPALNTVGG
GGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAKDEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIV
AADLIKVINKVKYECNVKIYYNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV
VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYCVNPMVIRENNKLDLVLPVRK
K

Specific function: This enzyme methylates the first cytosine within the sequences GGCC and GCNGC [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=208, Percent_Identity=28.8461538461538, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 64656; Mature: 64656

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVW
CEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRNLPYADLLVWGFPCPDISVAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKN
CCCCCHHHEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
LVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNRERVFIVSIRKDIDTGIFSFP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHCCC
KGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA
CCCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHH
LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNP
EECCCCCEEEECCCCCHHHHHHCCCCHHCCCCEEEEEECCCCCCEEEECCEEECCCCCCC
QRGRIYSPDGISPALNTVGGGGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAK
CCCEEECCCCCCHHHHCCCCCCCCCHHEEEECCHHHEECCCHHHHHHHCCCCCHHHHHHH
DEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIVAADLIKVINKVKYECNVKIY
HHHHHHHCCEEECCCCEEEECCEEEEEECCCCCCCCCEEEHHHHHHHHHHCCEEEEEEEE
YNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV
ECCCEEEEEECCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEE
VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYC
EEEECCCCEEEEEECCCEEEEEEEEEEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEEE
VNPMVIRENNKLDLVLPVRKK
CCEEEEECCCCEEEEEECCCC
>Mature Secondary Structure
MLKVRTLFSGIGSPERALKDLQIPYELVDFCEVDKYAVKSYCSVHGVSEEKNLGDITKVW
CEEEEEHHHCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCHHHHHH
GRNLPYADLLVWGFPCPDISVAGKQKGIKEGETRSGLYYEGFRILKETRPKYSIIENVKN
CCCCCHHHEEEECCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
LVGKRFKADFESMLEDIESLGYNNYWKVLNAKDYGIPQNRERVFIVSIRKDIDTGIFSFP
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCCCHHCCC
KGFDNGLRLKDFLENEVDEKYYISEKATQGLLRALKRPHTPKMLNKDSEYSPTLDTRVGA
CCCCCCCCHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCHHHHHHH
LTHRSPYMCIPCITPDRAEKRQNGRRFKENGDPMFTLTSQDRHGILQVGNIVDTGNWGNP
EECCCCCEEEECCCCCHHHHHHCCCCHHCCCCEEEEEECCCCCCEEEECCEEECCCCCCC
QRGRIYSPDGISPALNTVGGGGLEPKILKYKRTEYRIRKLTPKECWRLMGFTDEDFQKAK
CCCEEECCCCCCHHHHCCCCCCCCCHHEEEECCHHHEECCCHHHHHHHCCCCCHHHHHHH
DEIRPILNGICINKNEFIALDGYRLALRKGNFETKEPVIVAADLIKVINKVKYECNVKIY
HHHHHHHCCEEECCCCEEEECCEEEEEECCCCCCCCCEEEHHHHHHHHHHCCEEEEEEEE
YNDNYVKFKFDDLEIVGYRRQGNYINYNSIIPENYNTKVTLKIKPILDILKDYKKNKFKV
ECCCEEEEEECCEEEEEEEECCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHCCCCEEE
VDLDFQNDKLIIKANNDIATVEESITIELQGEPLEISFNADYLIDAFKNYDNATLELTYC
EEEECCCCEEEEEECCCEEEEEEEEEEEECCCEEEEEECCHHHHHHHHCCCCCEEEEEEE
VNPMVIRENNKLDLVLPVRKK
CCEEEEECCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]