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The map label for this gene is dapG

Identifier: 170757733

GI number: 170757733

Start: 2527753

End: 2528958

Strand: Reverse

Name: dapG

Synonym: CLD_2231

Alternate gene names: 170757733

Gene position: 2528958-2527753 (Counterclockwise)

Preceding gene: 170756878

Following gene: 170754867

Centisome position: 63.89

GC content: 31.84

Gene sequence:

>1206_bases
ATGAAAATCTTAATTCAAAAGTTTGGAGGAACATCTGTATCTACAGCTGAAAGAAGATCTTTAGTAGTAGATAAAATAGT
TAAGGCAAAAAAAGCAGGTTATTATCCAGTGGTGGTAGTATCTGCTATGGGTAGAAAAGGACAGCCTTATGCTACAGACA
CATTGAGATCTTTGGTTGGAGAAGACTTTTTAGATAAAAATACCTTAGCTGCTGATCTTTTAATGGGTTGCGGTGAATTA
ATAAGCACAGTAGTTATGAGCTCAGAACTTTTTAATAAGGGAATAGATGCAGTACCTTTAATGGGAGGACAGGCAGGTAT
TATAACAGATAATAATTTTAACAATGCTTCTGTACTTAGAGTAGAAAAAGATAGAATAGTAGATTTACTGAAAAAAGATA
AAATTCCTGTAGTGGCAGGTTTTCAAGGGAAAAGTGAAGATGGTTATATAACTACCCTAGGAAGAGGTGGCAGTGATGTT
ACTGCCGCACTTTTAGGAGCTGCATTAGAGGCTGAAAGTGTAGAAATATATACGGATGTAGATGGTATAATGACTGCAGA
TCCTAGAATAGTAGAGAATGCCTCCTTAATAAAAGAAATAAGTTATAATGAGGTGTTTCAATTTGCAGATCAAGGGGCTA
AGGTAATACATCCAAGAGCAGTAGAAATTGCCATGACGGCAAATACAAAACTTGTAATAAAAAACACTATGACAGATTGC
AAAGGTACCACTATAAATAATATAGGAATTAAAAATTCAAATAATGTTATAACAGGTATTACCCATATGAGTAATAGAAC
GCAAATTATAGTAGATGCAGAAGAAAATAAAGGAAATAAAAACTATACTAACCTATTAAATTCATTGGCAGAAAATTCTA
TTAGTATTGACCTTATAAATGTTTTTCCTAAAGAAAAAATATTCACTATAGATGAAAAAGATTTTAATGAGTTTAGTTCT
ATAATGGAAGGTTTAAAAATAAAATTTTTCTACTTAAAAGATTGTAGTAAAATAGCCATAATAGGTAGTAGAATGAGAGG
GATACCTGGGGTTATGGCTAAAATATTAAAGGCACTTGTAGAAAGAAATATAGAAGTGCTTCAAACAGCAGACTCGCATA
CAACTATATGGTGTCTTGTTTCTAAGGAAGATACAGAAAAAGCTATAAAATCATTGCACTGTGAATTTAAGTTAGATTGT
ATATAA

Upstream 100 bases:

>100_bases
AGTGTCTTGGGACTCTGTAAAAAAAATAGGATCAAGAACTATAATAATTGATGCTGAAAAGAGAGAATTAAAAAAAATCC
GCTAGGGAGATGGTAGACAA

Downstream 100 bases:

>100_bases
ACAAAATTCTTAGCTTCAGGTGGAATTTTAACTCAAATTTGAGCTAAGAAGTGCTTAGATAGGTAATATCAGACATTATC
TCTAACTTTGTAAGTGGGAG

Product: aspartate kinase I

Products: NA

Alternate protein names: Aspartate kinase 1; Aspartokinase I [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MKILIQKFGGTSVSTAERRSLVVDKIVKAKKAGYYPVVVVSAMGRKGQPYATDTLRSLVGEDFLDKNTLAADLLMGCGEL
ISTVVMSSELFNKGIDAVPLMGGQAGIITDNNFNNASVLRVEKDRIVDLLKKDKIPVVAGFQGKSEDGYITTLGRGGSDV
TAALLGAALEAESVEIYTDVDGIMTADPRIVENASLIKEISYNEVFQFADQGAKVIHPRAVEIAMTANTKLVIKNTMTDC
KGTTINNIGIKNSNNVITGITHMSNRTQIIVDAEENKGNKNYTNLLNSLAENSISIDLINVFPKEKIFTIDEKDFNEFSS
IMEGLKIKFFYLKDCSKIAIIGSRMRGIPGVMAKILKALVERNIEVLQTADSHTTIWCLVSKEDTEKAIKSLHCEFKLDC
I

Sequences:

>Translated_401_residues
MKILIQKFGGTSVSTAERRSLVVDKIVKAKKAGYYPVVVVSAMGRKGQPYATDTLRSLVGEDFLDKNTLAADLLMGCGEL
ISTVVMSSELFNKGIDAVPLMGGQAGIITDNNFNNASVLRVEKDRIVDLLKKDKIPVVAGFQGKSEDGYITTLGRGGSDV
TAALLGAALEAESVEIYTDVDGIMTADPRIVENASLIKEISYNEVFQFADQGAKVIHPRAVEIAMTANTKLVIKNTMTDC
KGTTINNIGIKNSNNVITGITHMSNRTQIIVDAEENKGNKNYTNLLNSLAENSISIDLINVFPKEKIFTIDEKDFNEFSS
IMEGLKIKFFYLKDCSKIAIIGSRMRGIPGVMAKILKALVERNIEVLQTADSHTTIWCLVSKEDTEKAIKSLHCEFKLDC
I
>Mature_401_residues
MKILIQKFGGTSVSTAERRSLVVDKIVKAKKAGYYPVVVVSAMGRKGQPYATDTLRSLVGEDFLDKNTLAADLLMGCGEL
ISTVVMSSELFNKGIDAVPLMGGQAGIITDNNFNNASVLRVEKDRIVDLLKKDKIPVVAGFQGKSEDGYITTLGRGGSDV
TAALLGAALEAESVEIYTDVDGIMTADPRIVENASLIKEISYNEVFQFADQGAKVIHPRAVEIAMTANTKLVIKNTMTDC
KGTTINNIGIKNSNNVITGITHMSNRTQIIVDAEENKGNKNYTNLLNSLAENSISIDLINVFPKEKIFTIDEKDFNEFSS
IMEGLKIKFFYLKDCSKIAIIGSRMRGIPGVMAKILKALVERNIEVLQTADSHTTIWCLVSKEDTEKAIKSLHCEFKLDC
I

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartokinase family [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=462, Percent_Identity=27.0562770562771, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1790455, Length=266, Percent_Identity=29.3233082706767, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790376, Length=177, Percent_Identity=28.8135593220339, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=364, Percent_Identity=23.9010989010989, Blast_Score=102, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 43802; Mature: 43802

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIQKFGGTSVSTAERRSLVVDKIVKAKKAGYYPVVVVSAMGRKGQPYATDTLRSLVG
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHC
EDFLDKNTLAADLLMGCGELISTVVMSSELFNKGIDAVPLMGGQAGIITDNNFNNASVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCEEEEE
VEKDRIVDLLKKDKIPVVAGFQGKSEDGYITTLGRGGSDVTAALLGAALEAESVEIYTDV
ECHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECC
DGIMTADPRIVENASLIKEISYNEVFQFADQGAKVIHPRAVEIAMTANTKLVIKNTMTDC
CCEEECCCCHHHCHHHHHHCCHHHHHHHHHCCCEEECCCEEEEEEECCCEEEEEECCCCC
KGTTINNIGIKNSNNVITGITHMSNRTQIIVDAEENKGNKNYTNLLNSLAENSISIDLIN
CCCEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEE
VFPKEKIFTIDEKDFNEFSSIMEGLKIKFFYLKDCSKIAIIGSRMRGIPGVMAKILKALV
ECCCCEEEEECCCCHHHHHHHHCCCEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHH
ERNIEVLQTADSHTTIWCLVSKEDTEKAIKSLHCEFKLDCI
HCCEEEEEECCCCEEEEEEEECCHHHHHHHHHCCCEEEECC
>Mature Secondary Structure
MKILIQKFGGTSVSTAERRSLVVDKIVKAKKAGYYPVVVVSAMGRKGQPYATDTLRSLVG
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHC
EDFLDKNTLAADLLMGCGELISTVVMSSELFNKGIDAVPLMGGQAGIITDNNFNNASVLR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEECCCCCCEEEEE
VEKDRIVDLLKKDKIPVVAGFQGKSEDGYITTLGRGGSDVTAALLGAALEAESVEIYTDV
ECHHHHHHHHHHCCCCEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECC
DGIMTADPRIVENASLIKEISYNEVFQFADQGAKVIHPRAVEIAMTANTKLVIKNTMTDC
CCEEECCCCHHHCHHHHHHCCHHHHHHHHHCCCEEECCCEEEEEEECCCEEEEEECCCCC
KGTTINNIGIKNSNNVITGITHMSNRTQIIVDAEENKGNKNYTNLLNSLAENSISIDLIN
CCCEEECCCCCCCCCEEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEE
VFPKEKIFTIDEKDFNEFSSIMEGLKIKFFYLKDCSKIAIIGSRMRGIPGVMAKILKALV
ECCCCEEEEECCCCHHHHHHHHCCCEEEEEEECCCCEEEEEHHHHCCCHHHHHHHHHHHH
ERNIEVLQTADSHTTIWCLVSKEDTEKAIKSLHCEFKLDCI
HCCEEEEEECCCCEEEEEEEECCHHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8098035; 9384377 [H]